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Citations for Roche :
2101 - 2150 of 6956 citations for 6 IODO 2 PIPERAZIN 1 YL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Medium calcium content was measured by photometrical absorption measurement of calcium EDTA complex using a Calcium Gen.2 kit (Roche, Germany). After 7 days ...
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bioRxiv - Neuroscience 2021Quote: ... the L4/5 DRG were rapidly harvested from the isoflurane anesthetized naïve rats and were incubated in 0.01% blendzyme 2 (Roche Diagnostics, Madison, WI) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: TUNEL staining including antigen retrieval (microwave 5min x 2) and staining process was performed following manufacturer’s instruction (11864795910, Roche Applied Science). FITC-labeled TUNEL-positive cells were imaged under fluorescent microscopy ...
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bioRxiv - Microbiology 2022Quote: ... Immunoreactivity in the lung tissues was assessed using a rabbit anti-SARS-CoV-2 Nucleocapsid pAb as the primary antibody (Sinobiological, China) and further processed using a Ventana Discovery Ultra (Roche, USA) system.
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bioRxiv - Molecular Biology 2019Quote: ... Dehydrated slides were then placed on coverslips containing 70 µL hybridization mix (10 mM Tris-HCl, 2% blocking reagent (Roche, #11096176001), 70% formamide and 0.1 µM Cy3 labeled (CCCTAA)3 PNA probe (PNA Bio ...
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bioRxiv - Biochemistry 2019Quote: ... and the resulting duplex was captured on 3 mg streptavidin-coated magnetic beads (Roche, rotation for 2 h at 37°C). After extraction with phenol-chloroform and precipitation with ethanol ...
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bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
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bioRxiv - Immunology 2021Quote: The viral load was inferred on nasopharyngeal swabs through cycle threshold (Ct) determination with Cobas® SARS-CoV-2 Test (Roche), a real-time PCR dual assay targeting ORF-1a/b and E-gene regions on SARS-CoV- 2 genome ...
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bioRxiv - Genetics 2021Quote: ... The cell pellet was resuspended in buffer 2 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% glycerol, Roche Complete Protease Inhibitor), incubated for 10 min at 4°C followed by centrifugation to collect nuclei ...
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bioRxiv - Genetics 2021Quote: ... The cell pellet was resuspended in buffer 2 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% glycerol, Roche Complete Protease Inhibitor), incubated for 10 min at 4°C followed by centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... were amplified using primers Sb.1 - Sb.2 and Sb.3 to Sb.8 (Table 2) and labeled with digoxigenin (DIG) using the PCR DIG probe synthesis kit (Roche Diagnostics). Membranes were hybridized with these probes at 65°C and then washed at 68°C with decreasing concentrations (from 2X to 0.2X ...
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bioRxiv - Cancer Biology 2020Quote: Cell proliferation was measured by determining the extent of 5-Bromo-2’-deoxy-uridine (BrdU) incorporation into DNA of U87-MG cells using the BrdU cell proliferation assay ELISA kit (Roche, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... cells were removed from activation and mixed at a 1:1 volume ratio with complete T cell media containing recombinant IL-2 (obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH from Dr. Maurice Gately, Hoffmann - La Roche Inc), to give a final IL-2 concentration of 20 units/mL ...
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bioRxiv - Microbiology 2021Quote: ... primers and probe specific for SARS-CoV-2 RdRP (TibMolBiol; #53-0777-96) and a LightCycler Multiplex RNA Virus master kit (Roche; #07083173001) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... Roche Elecsys® Anti-SARS-CoV-2 Immunoassay for the qualitative detection of antibodies (including IgG) against SARS-CoV-2 was used with a COBAS e411 analyzer (disk system; Roche, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... ifn-β and bcl-2 genes were amplified with specific primers and quantified by qPCR using a LightCycler 96 system (Roche). The mRNA levels of il-6 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 volume of nuclei suspension was mixed with 1 volume of DNase I cocktail (NWB supplemented with CaCl2 2 mM, BSA 100 μg/ml, and twice the indicated concentration of DNase I (Roche 10104159001) and incubated for 8 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... IFNγ-R2-eGFP plasmid was transfected in HeLa or Caco-2 cells with X-tremeGENE HP DNA transfection reagent (Roche, 6366236001) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... brucei cells were washed twice with PBS and lysed in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EGTA, 0.2% NP-40, Roche Complete protease inhibitor) for 20 min at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 2 mM CaCl2) containing 0.3 M of sucrose and MNase (30 units of MNase for every 2 million sperm, Roche Nuclease S7) was added to each tube ...
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bioRxiv - Immunology 2022Quote: ... IL-17RA KO or IL-17A/F DKO mice injected with B16F10-SIY.GFP and EL4-SIY.GFP and disaggregated first mechanically and then enzymatically with 2mL of RPMI containing 2 mg/mL collagenase IV (Roche, cat: 11088882001) and 50 U/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2022Quote: Mouse hippocampal proteins from PBS control and Aβ oligomer (JD1 positive) treated brain slices were extracted with 2% SDS (in PBS) containing protease and phosphatase inhibitors (Roche, Switzerland) followed by centrifugation at 14000 x g for 15min to precipitate any insoluble material ...
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bioRxiv - Systems Biology 2023Quote: ... HepG2s were washed once with 1X PBS then lysed in SDS lysis buffer (50 mM Tris HCl/2% SDS/5% glycerol/5 mM EDTA/1mM NaF/dH2O) supplemented with cOmplete Protease Inhibitor Cocktail Tablets (Roche #11836170001), Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich® #P5726) ...
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bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (solely used for CDKN1A ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (hereafter called qPCR) was performed by using the KAPA SYBR FAST qPCR Kit Master Mix (2×) Universal (KAPA Biosystems) with StepOne plus (Applied Bio systems) ...
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bioRxiv - Cell Biology 2023Quote: ... Three trachea from either wild-type or Trp73-/- mice were pooled together in 15 mL falcon tubes containing 2 mL 0.15% filter sterilised Pronase (Roche, Basel, Switzerland) and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche, 04379012001), and a 370-bp fragment (nucleotides 1304- 1673 of NM_010610.3 ...
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bioRxiv - Microbiology 2023Quote: ... The elution fractions were collected in tubes that were previously supplemented with 2 µl Protector RNase inhibitor (Roche Diagnostics, Karlsruhe, Germany). The elution fractions were split ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies were visualized chromogenically by incubating the membrane in an alkaline phosphatase buffer (130mM Tris, pH 9, 150 mM NaCl, 100 μM MgCl2) and 2% BCIP/NBT (Roche: 11681451001).
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Immunology 2023Quote: ... coated with 2.5ug/mL aCD3 and re-stimulated for an additional 3 days in complete IMDM-10 supplemented with 50U/mL IL-2 (Roche 11011456001). For Th17 polarizations ...
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bioRxiv - Microbiology 2024Quote: ... BHK cells were transfected with 3 μg of pBAC-SARS-COV-2 construct and1 μg of SARS-CoV-2 N (Delta) plasmid with the X-treme Gene 9 (X9) DNA transfection reagent (Roche: 6365809001) was added 1:3 as DNA:X9 ...
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mm EDTA in ddH2O) supplemented with 1:25 cOmplete Protease Inhibitor Cocktail (Roche Diagnostic, catalog #11697498001) and 1:200 PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... 1% NP-40 for 10 min in the presence of complete protease inhibitor cocktail (Roche, 1:1000) on ice ...
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bioRxiv - Microbiology 2019Quote: ... 1 h) and labeled with primary antibodies at the following dilutions: rat anti-HA (1:1000; Roche), mouse anti-Myc (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... 1% sodium deoxycholate and 1% Triton X-100) supplemented with 1xEDTA-free complete protease inhibitor mixture (Roche) and phosphatase inhibitor cocktails-I ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5 mM MgCl2) with 1 mM PMSF and 1× EDTA-free protease inhibitor cocktail (PIC, Roche, 11873580001) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... in blocking buffer for 1 hour at room temperature followed by 1 hour of DAPI (Roche, # 33495822) staining ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was then resuspended in HEGX Buffer (20 mM HEPES-KOH, pH 7.2, 0.8M NaCl, 1 mM EDTA, 10% glycerol, 0.2% Triton X-100 and 1 Roche Complete Protease Inhibitor Tablet ...
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bioRxiv - Genomics 2022Quote: ... 1 μL of 1:10 diluted cDNA was used in a 10 μL SYBR Green (Roche, 04887352001) qPCR reaction on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1:500 mouse anti-actin26 and 1:1000 rat anti-HA (3F10, Roche) followed by 1:1000 anti-mouse Alexa 488 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 1 % Nonidet-P 40) supplied with 1 tablet/50 mL c0mplete EDTA-free Protease Inhibitor Cocktail (Roche), per mg cell pellet and rotating on a wheel at 4°C for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Purified parasites treated at 12°C for 1 h with freshly prepared 1 mg/mL pronase (Roche)/0.01% saponin/PBS before washing ...
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miRNA activity contributes to accurate RNA splicing in C. elegans intestine and body muscle tissues.bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100 and 1 tablet of protease inhibitor for every 10ml of lysis buffer (Roche cOmplete ULTRA Tablets ...
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bioRxiv - Neuroscience 2019Quote: ... 1% Triton X-100 and 0.1% SDS) with protease inhibitor (Roche/1 tablet in 10 mL RIPA). Sample homogenates were centrifuged for 5 min at 4°C at 13,000 G ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% NaDOC, 1% Triton X-100, 0.1% SDS, 0.25% sarkosyl, 1 mM DTT, protease inhibitors from Roche). The cells were sonicated on ice to shear DNA to 300-500 bp ...