Labshake search
Citations for Roche :
1651 - 1700 of 5869 citations for Mouse Serine threonine Protein Kinase 17B STK17B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Protein lysate of 10-20 μg was run on 10–15% SDS-PAGE gel and transferred toa PVDF membrane (Roche, USA). The membrane was incubated for 60 min at room temperature in 5% BSA solution ...
-
bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
-
bioRxiv - Bioengineering 2020Quote: Western blotting was performed as previously reported.[26] Protein extracts were prepared using RIPA buffer containing EDTA protease (Roche Applied Science) and phosphatase inhibitors (Phostop Roche) ...
-
bioRxiv - Neuroscience 2021Quote: ... mice and lysed on ice for 30 min in protein lysis buffer (1 mM Na3VO4, 1 mM NaF, complete protease inhibitor cocktail (Roche, USA), 1% Triton X-100 in PBS) ...
-
bioRxiv - Plant Biology 2020Quote: ... Resulting yeast extracts were used for western blot analysis using anti-Myc HRP-DirecT (MBL, Woburn, MA, United States) for bait proteins and anti-HA (3F10)-HRP (Roche, Switzerland) for prey proteins.
-
bioRxiv - Immunology 2021Quote: Secreted twin strep-tagged gp140-soc proteins in the harvested and filtered supernatant (1L) were supplemented with protease inhibitor tablets (Roche Diagnostics) to prevent protein degradation and 5 ml of BioLock biotin blocking solution (iba Life Sciences GmbH ...
-
bioRxiv - Systems Biology 2019Quote: ... The cross-linked proteins-RNA were cut directly from the gel and incubated with 160 μg of Proteinase K (Roche, 3115801001) in 600 μl wash buffer 2 supplemented with 1% SDS and 5 mM EDTA at 55°C for 2-3 hours with mixing ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were extracted from RAW264 using radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Complete Mini, Roche Diagnostics GmbH, Mannheim, Germany) and phosphatase inhibitors (PhosSTOP EASYPack ...
-
bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
-
bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
-
bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
-
bioRxiv - Genetics 2022Quote: ... 10 μg total proteins were separated on a 12% SDS-PAGE gel and transferred to the PVDF membranes (Roche, CA, USA) using an electro-transfer apparatus (Bio-Rad) ...
-
bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... Hand dissected ovaries from 100-150 flies (depending on the expressed protein) of appropriate genotypes were lysed in RIPA buffer supplemented with Complete Protease Inhibitor cocktail (Roche, 11836170001) and 20 mM NEM ...
-
bioRxiv - Neuroscience 2023Quote: Proteins were detected by Western blot analysis after separating 30–50 μg of total protein lysate prepared in RIPA buffer with protease inhibitor cocktail (cOmplete™, Roche) on a 4– 15% Mini-PROTEAN® TGX™ Precast Gel (BioRad ...
-
bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the protein lysates were prepared in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor mixture (Roche Applied Science, IN, USA) and 2mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
-
bioRxiv - Genetics 2023Quote: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251) ...
-
bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
-
bioRxiv - Molecular Biology 2023Quote: ... containing either single (for the IgG screen) or serial dilutions (for full IgG multi-point kinetics) of HEL protein (Roche #10837059001). For measurement of Fab binding kinetics ...
-
bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
-
bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
-
bioRxiv - Neuroscience 2023Quote: ... pH 7.4 with 0.1% Triton X-100) and then incubated with 350 µg protein in 1:1 8M urea with Complete protease inhibitor (Roche 11245200) sample lysis:Buffer A/0.5% Triton X-100 (150 mM NaCl ...
-
bioRxiv - Neuroscience 2024Quote: Weighed brain tissue samples were processed for protein assays by addition of 10X volume NP-40 buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 mg nuclear lysate was adjusted to 500 uL in Protein Incubation Buffer (PIB+++; 150mM NaCl, 50 mM Tris pH 8.0, cOmplete protease inhibitor (Roche, cat 04693159001), 0.25% NP-40 and 1 mM DTT) ...
-
bioRxiv - Microbiology 2023Quote: ... The pooled library was cleaned using MO BIO UltraClean® PCR Clean-Up Kit (MO BIO, Carlsbad, CA) and quantified using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing was carried out as detailed in the EMP protocol ...
-
bioRxiv - Microbiology 2021Quote: ... pooled and quantified (KAPA library quantification kit from KAPA Biosystems), and sequenced with the MiSeq system (Illumina).
-
bioRxiv - Developmental Biology 2021Quote: ... and by qPCR using a KAPA Library Quantification Kit (Roche) for Illumina platforms ...
-
bioRxiv - Cell Biology 2020Quote: ... Revelation was performed using the Ultraview DAB revelation kit (Roche). Nuclei were counterstained with hematoxylin solution (Dako) ...
-
bioRxiv - Developmental Biology 2021Quote: ... quantified with a Kapa Library Quantification Kit (Kapa Biosystems KK4824), and sequenced on an HiSeq 2500 platform (Illumina ...
-
bioRxiv - Genetics 2021Quote: ... and libraries were prepared using KAPA HyperPrep Kit (Roche, 07962347001) using KAPA DUI adapters (Roche ...
-
bioRxiv - Microbiology 2021Quote: ... and the MagNA Pure Compact DNA Isolation Kit I (Roche) following the protocol “Total_NA_Plasma_external_lysis purification protocol” ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... using Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
-
bioRxiv - Developmental Biology 2020Quote: ... TUNEL labeling (In Situ cell death detection kit, Fluorescein, Roche) was performed according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: Libraries were prepared using the KAPA mRNA HyperPrep kit (Roche) and sequenced as 76bp single-end ...
-
bioRxiv - Molecular Biology 2021Quote: ... Libraries were quantified by KAPA library quantification kit (KAPA biosystems) and then multiplexed and sequenced on Illumina Hiseq 4000 platform to obtain 150 nt paired-end reads.
-
bioRxiv - Bioengineering 2022Quote: ... RNA was extracted using High-Pure RNA Isolation Kits (Roche) and assessed for concentration and purity using the NanoDrop 2000c – UV- Vis spectrophotometer ...
-
bioRxiv - Developmental Biology 2022Quote: ... using the In Situ Cell death detection Kit (Roche, 12156792910). All samples were counterstained in 4’,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Genomics 2020Quote: ... using Kapa HyperPlus KK8514 library kit (Kapa Biosystems, Wilmington, MA). DNA was enzymatically sheared to approximately 600bp fragments ...
-
bioRxiv - Genomics 2020Quote: ... PCRs were performed using KAPA2G Robust PCR kits (KAPA Biosystems). A typical 25 μl reaction used 20–100 ng of genomic DNA ...
-
bioRxiv - Developmental Biology 2019Quote: ... and detected with the DIG Nucleic Acid Detection Kit (Roche). Wing imaginal discs were mounted in glycerol and imaged with a Nikon E200 bright-field microscope.
-
bioRxiv - Microbiology 2020Quote: ... the KAPA Stranded mRNA-Seq library preparation kit (Kapa Biosystems) was used to prepare library samples and sequenced on the NextSeq platform (Illumina ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... or the KAPA Library Quantification Kit for Illumina (KAPA Biosystems). Sequencing was performed on Illumina instruments using the HiSeq SBS Kit v4 250 cycles kit (Illumina ...
-
bioRxiv - Genomics 2019Quote: ... Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems) was used to quantify RNA libraries by qPCR ...
-
bioRxiv - Microbiology 2019Quote: ... with the KAPA HiFi HotStart ReadyMix PCR Kit (KAPA biosystems), in a volume of 25 µl ...
-
bioRxiv - Molecular Biology 2020Quote: ... prepared for sequencing using Kappa HyperPrep Kits (Roche, Indianapolis, IN) and sequenced on a single MiSeq (Illumina ...
-
bioRxiv - Plant Biology 2019Quote: ... using the Library Quantification Kit (Roche Molecular Systems, Pleasanton, CA). Bisulfite and RNA sequencing reactions were performed on a NextSeq500 using a HighOutput chemistry ...
-
bioRxiv - Developmental Biology 2020Quote: In-situ cell Death Detection kit Fluorescein (Roche/ Sigma-Aldrich) was used to detect apoptotic cells ...
-
bioRxiv - Genomics 2019Quote: ... All libraries were quantified by Library Quantification Kit (Kapa Biosystems). The pools were sequenced using TruSeq SBS Kit v3-HS (Illumina Inc.) ...