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Citations for Roche :
1601 - 1650 of 5869 citations for Mouse Serine threonine Protein Kinase 17B STK17B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were detected using horseradish peroxidase-conjugated and detection with the Lumi- Light Plus Western Blotting Substrate (Roche). Luminescence was detected with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein purification started by quickly thawing the cells in ∼15 mL room-temperature 10mM imidazole with protease inhibitor cocktail (Roche) and freshly added 200µM PMSF ...
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bioRxiv - Cell Biology 2020Quote: Cell proteins were extracted by Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Scientifi, 88804) or Complete™ Lysis-M EDTA-free kit (Roche, 04719964001) according to the manuals ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of beta-globin was performed by a commercially available human genomic DNA kit (The LightCycler Control Kit DNA, Roche Diagnostics, Basel, Switzerland)69.
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bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: RNA-seq libraries and libraries containing both ATAC-seq and TaPE-seq were quantified by qPCR using the KAPA Library Quantification Kit (Complete kit, Universal) (F. Hoffmann-La Roche AG, Basel, Switzerland) on the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories Inc ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products were purified by using a High Pure PCR product purification kit (B A High Pure PCR Product Purification Kit (Roche Diagnostics, Mannheim, Germany), and purified products were sequenced directly using an ABI BigDye Terminator Cycle Sequencing Kit ver ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA libraries were created from 150 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboErase kit (Roche Diagnostics, Vilvoorde, Belgium), according to the manufacturer’s directions ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA was used for NGS library preparation using either the KAPA Library Preparation kit or the KAPAHyperPlus kit (KAPA Biosystems, MA, USA). Genomic libraries were captured with the HyperPlus Capture Exome Kit or the xGen Exome Panel V2 (Integrated DNA Technology ...
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... three to four-week old mice were euthanized and the mouse ventral forelimb skin was dissected for dissociation in Dispase II (Roche) to isolate epidermal whole mount preparations ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... and cell suspensions were prepared by digesting the mouse skin tissue in a cocktail consisting of Liberase TL (Roche, 5401020001) and DNase I (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-BALL (Gift from A. Herzig, IF 1:20, ChIP: 2μl) mouse anti-GFP (Roche, 11814460001, IF:1:50), mouse anti-Tubulin (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed to determine relative gene expression in pigmented versus non-pigmented mouse melanocytes using a LightCycler 480 instrument (Roche) with PrimeTime Gene Expression Master Mix (IDT DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... eGFP-tagged readthrough reporter was immunoprecipitated by addition of 10 μg of mouse monoclonal anti-GFP IgG (Roche, cat#11814460001) or normal mouse IgG as a negative control (Proteintech ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were then probed for 16 h at 4 °C with the following primary antibodies in 3% (w/v) skim milk in PBS: mouse anti-GFP (1:1000, Roche), rabbit anti-SBP1 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... the cortical and hippocampal tissues were dissected from mouse brains and homogenized in ice-cold RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche), followed by centrifugation at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 60 μl of anti-mouse magnetic beads were washed PBS/BSA (5 mg/ml BSA) and incubated with 3 μl of anti-Myc (Roche) or Anti-Rpb3 antibodies (Neoclone ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of Capture-C library per sample was mixed with mouse COT1 DNA and universal as well as index-specific blocking oligos from SeqCap EZ HE-oligo (Roche). 4.5 µl pool of biotinylated probes (xGen Lockdown Probe Pools from IDT) ...
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bioRxiv - Plant Biology 2022Quote: GFP was detected on the membranes using anti-GFP mouse-IgG monoclonal (clones 7.1 and 13.1) antibody (Roche, Basel, Switzerland) in a 1/500 dilution in 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Specific primers for the different genes of interest were designed using either the Universal ProbeLibrary (ProbeFinder version 2.5 for mouse, Roche Diagnostics) or PrimerBlast (NCBI ...
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bioRxiv - Genetics 2022Quote: DNA sequences corresponding to the different donor/daughter mouse L1 elements were amplified from genomic DNA using Expand Long Range dNTPack (Roche). Reaction mixes contained 5 μl 5x Expand Long Range Buffer with 12.5 mM MgCl2 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were in contact for 2 hours at room temperature with a mouse monoclonal primary antibody anti-BrdU (1/1000 dilution) (Roche), then rinsed and incubated for 30 min with a fluorescent secondary antibody Alexa-633nm goat anti-mouse (Invitrogen ...
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bioRxiv - Physiology 2019Quote: ... Cartilages from two hips of one mouse were pooled together and washed 3 times in PBS with protease (Roche 11836170001) and phosphatase inhibitors (Sigma P0044 and P5726 ...
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bioRxiv - Genomics 2021Quote: ... 1μg of Capture-C library per sample was mixed with mouse COT1 DNA and universal as well as index-specific blocking oligos from SeqCap EZ HE-oligo (Roche). 4.5μl pool of biotinylated probes (xGen Lockdown Probe Pools from IDT) ...
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bioRxiv - Molecular Biology 2021Quote: ... were sampled at the apex of liver in mouse lemurs under general anesthesia (induction with Diazepam 0.05 mg/kg, Valium®, Roche; Ketamine 0.2 mg/kg ...
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bioRxiv - Immunology 2020Quote: ... mouse thymus was collected and homogenized in 1 ml of cold PBS containing cOmplete protease inhibitor cocktail (Roche, Indianapolis, IN) using a tissue homogenizer (Omni International ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were hybridised with monoclonal antibodies against GFP followed by anti-mouse alkaline-phosphatase conjugated antibody and signals were developed using NBT-BCIP (Roche).
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bioRxiv - Neuroscience 2022Quote: ... brains were incubated with primary antibodies (1:200, chicken anti-GFP, Abeam, Cat# ab13970; 1:200, mouse anti-GFP, Roche, Cat# AB_390913 ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization signals were detected with anti-mouse secondary antibody labeled with alkaline phosphatase using NBT/BCIP substrates (Roche Biochemical, Mannheim).
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bioRxiv - Developmental Biology 2022Quote: Dorso-lateral skins of E14.5 C57BL/6JOlaHsd mouse embryos were dissected and enzymatically separated using 2.5 U/ml Dispase II (Roche, Tokyo, Japan) in 4°C ...
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bioRxiv - Immunology 2022Quote: Mouse’s left lung was cut into pieces and digested for 1h with 10mL of DNAse I (10mg/mL, Roche #10104159001) and type I collagenase (100mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Sections of mouse brain were homogenized in 10 volumes (w/v) of TBS buffer with a cOmplete protease inhibitor cocktail (Roche), and centrifuged at 1000,000 × g for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
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bioRxiv - Evolutionary Biology 2023Quote: ... lower and upper mouse and hamster first molars were dissected as above and treated for 15 minutes at 37°C with Dispase (Roche) 10mg/ml in Hepes/KOH 50mM ph7.7 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 4°C overnight with the primary antibody mouse IgG1 Anti-Tbx6 (home-made, clone A83, dilution 1:500) in 2% Blocking Reagent (Roche) and 5% sheep serum (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: Footpads and tips of mouse digits were dissected under a stereoscope microscope and digested for 45 min in 1g liberase TL (Roche)/1mL HBSS with calcium at 37C ...
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bioRxiv - Neuroscience 2023Quote: Target sequences detecting Ncan and Tnc were amplified from mouse brain cDNA and cloned into the pSPT 19 vector (Roche). All primers used for probe amplification are listed in the Key Resources table ...
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bioRxiv - Physiology 2024Quote: ... FDB muscles were extracted from mouse footpads and digested in 1.3 mg/ml collagenase A (Roche Diagnostics, Indianapolis, IN, USA) in Ringers solution containing 146 mM NaCl ...
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bioRxiv - Physiology 2023Quote: ... co-staining for Cluster of Differentiation 15 (CD15) antigen was carried out using the CONFIRM Anti-CD15 Mouse Monoclonal Primary Antibody (Ventana/Roche; Roche Diagnostics ...
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bioRxiv - Physiology 2023Quote: ... co-staining for Cluster of Differentiation 15 (CD15) antigen was carried out using the CONFIRM Anti-CD15 Mouse Monoclonal Primary Antibody (Ventana/Roche; Roche Diagnostics ...
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bioRxiv - Bioengineering 2024Quote: ... Primary fibroblast cultures were obtained by subjecting small pieces of mouse ear tissue to a 30-minute digestion with Collagenase/Dispase (Roche) at 37°C under agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples (containing 20 μg of total protein) were mixed with loading buffer and separated on 12 % SDS-PAGE gels and transferred to PVDF membranes (Roche, Switzerland). After blocking with Tris-buffered saline with 0.1% Tween 20 and 5 % nonfat dry milk ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting yeast extracts were used for immunoblot analysis using anti-Myc (MBL, Woburn, MA, United States) for bait proteins and anti-HA (Roche, Switzerland) for prey proteins ...