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Citations for Roche :
1901 - 1950 of 5869 citations for Mouse Serine threonine Protein Kinase 17B STK17B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The gene encoding this protein was amplified from genomic DNA of the SECphi4 phage using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601) with primers AS330+AS331 (Table S6) ...
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bioRxiv - Cancer Biology 2022Quote: Total protein cell extracts were obtained by lysing the cells with RIPA buffer supplemented with 1x protease inhibitors (PI) (Roche, Milan, Italy). Proteins were quantified using the BCA method (Pierce ...
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bioRxiv - Cancer Biology 2022Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were prepared using T-PER (Pierce Chemical Co., IL) in the presence of protease inhibitors (Roche Applied Science, Barcelona, Spain), and subjected to Western blot analyses with anti-rat anti-ABCA1 antiserum (kindly donated by Dr ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates of 20 μg protein were separated in a SDS-12% polyacrylamide gel (PAGE) followed by transferring proteins to a polyvinylidene difluoride (PVDF) membrane (Roche, catalog 3110040). After blocking in 5% milk (in 1xphosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2022Quote: Mouse liver tissues and cells were homogenized and lysed in Protein Extraction Reagent (P0013, Beyotime Biotechnology) supplemented with protease inhibitor cocktail (04693159001, Roche, Basel, Switzerland). Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 HUVEC were washed with CUT&RUN wash buffer (20 mM HEPES pH 7.9, 150 mM NaCl, 500 nM spermidine, 1X Roche Protein Inhibitor Cocktail) at RT ...
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bioRxiv - Microbiology 2024Quote: ... respectively) was split in two samples with similar protein concentration of which one was mixed with Complete Protease Inhibitor (Roche, Mannheim, Germany) and the other one with ddH2O as a control ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Microbiology 2024Quote: In-situ detection of DNA fragments following treatment of cells with MLT 5 μM and 10 μM for 24 h was measured by TUNEL using a Cell Death Detection kit (In-Situ Cell Death Detection Kit, POD; Roche; Cat. No. 11 684 817 910). 1000 μL of cells were centrifuged (3000 rpm for 5 min) ...
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bioRxiv - Microbiology 2020Quote: ... and further purified using the High Pure RNA Isolation kit (Roche). RNA quantity was assessed using the Quant-iT RiboGreen RNA Assay kit (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were constructed using the KAPA HyperPrep Kit (Kapa Biosystems, Switzerland) followed by sequencing on an Illumina NovaSeq6000 with 2×150 bp paired-ends.
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bioRxiv - Molecular Biology 2020Quote: ... Fibroblast proliferation was determined using colorimetric BrdU incorporation assay kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Assays were performed using the Luciferase Reporter Gene Assay Kit (Roche) to obtain data as relative light units (RLU) ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequent ligation (Rapid DNA Ligation Kit, Roche, Merck, Zug, CH). Then ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions with KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) were setup with 80ng genomic DNA ...
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bioRxiv - Genomics 2020Quote: ... Exome libraries were prepared using a Hyper Prep Kit (KAPA Biosystems), pre-capture PCR and SureSelectXT Human All Exon V5+UTR baits (Agilent) ...
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bioRxiv - Developmental Biology 2021Quote: ... The In Situ Cell Death Kit (TMR red; Roche Applied Science) was applied to fixed retinal sections as per the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Library concentration was determined using a KAPA Library Quantification Kit (Roche). Deep sequencing was performed on an Illumina NovaSeq 6000.
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bioRxiv - Genomics 2022Quote: ... two libraries were constructed using the Kapa Hyper Prep kit (Roche) according to Arima’s recommendation (Library Preparation using KAPA Hyper Prep Kit DOC A160108 v01) ...
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bioRxiv - Cell Biology 2019Quote: ... immunofluorescence and TUNEL assay (in situ cell death detection kit, Roche). Images were captured by Aperio (Leica Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP library was constructed by KAPA Hyper Prep Kit (KAPA BIOSYSTEMS) and SeqCap Adapter Kit A (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared using the KAPA Library Preparation Kit (KAPA Biosystems), and were amplified for a total of 12 PCR cycles (15 s at 98°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and quantification performed with a KAPA Library Quantification Kit (Roche, KK4844). Libraries were sequenced by the Babraham Institute Next Generation Sequencing Facility on an Illumina HiSeq2500 in Rapid Run 50bp Single End mode.
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bioRxiv - Genomics 2020Quote: ... with KAPA Library Quantification Kit (Kapa Biosystems Inc., Wilmington, MA, USA) for concentration ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were constructed using the KAPA Hyper Prep Kit (Roche) or NEBNext UltraII DNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Immunology 2019Quote: ... with the Light Cycler 480 SYBR Green detection kit (Roche Diagnostics) using the primers listed in Supplementary Table 2 ...
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bioRxiv - Physiology 2019Quote: ... and the DISCOVERY ChromoMab DAB kit (RUO) (Ref. 760-159, Roche). Sections were incubated with the primary antibody anti-Pecam1 (Ref ...
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bioRxiv - Genomics 2019Quote: ... and quantity measured with KAPA Library Quantification Kits (KK4835, KAPA Biosystems).
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bioRxiv - Genomics 2019Quote: ... and quantity measured with KAPA Library Quantification Kits (KK4835, KAPA Biosystems)
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bioRxiv - Microbiology 2019Quote: ... TUNEL-Fluorescein (In Situ Cell Death Detection Kit, Roche Life Science) was used to measure apoptosis at the single cell level following manufacturer’s recommendations ...
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bioRxiv - Physiology 2019Quote: RNA was extracted using the High Pure RNA Tissue Kit (Roche) following the manufacturer’s guidelines and the concentration determined using the Nanodrop 1000 spectrophotometer (ThermoScientific) ...
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bioRxiv - Physiology 2019Quote: ... RNA was extracted using the High Pure RNA Tissue Kit (Roche) following the manufacturer’s guidelines and the concentration determined using the Nanodrop 1000 spectrophotometer (ThermoScientific) ...
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bioRxiv - Genomics 2019Quote: ... and quantified with the KAPA Library Quantification Kit (Kapa Biosystems #KK4844), and then sequenced in a paired-ended manner using the NextSeq 500 (Illumina ...
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bioRxiv - Immunology 2019Quote: ... library preparation was performed using the Kappa Hyperprep kit (Roche Diagnostics) and sequencing was undertaken by the Pathogens Genomic Unit (UCL ...
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bioRxiv - Immunology 2019Quote: ... and quantified with Kapa Library Quantification Kit (Kapa Biosystems, Wilmington, MA). Libraries were sequenced by paired-end sequencing (2 × 150bp ...
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bioRxiv - Synthetic Biology 2019Quote: ... using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems). The libraries were then pooled at equimolar concentrations and sequenced on the Illumina MiSeq platform at a read-length of 300bp paired-end ...
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bioRxiv - Microbiology 2019Quote: ... DNA was purified using High Pure PCR Product Purification Kit (Roche), according to the recommendations of the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: ... and quantified using KAPA Library Quantification Kit for Illumina (KAPA Biosystems). Paired end 75 bp reads were obtained for pooled libraries using Illumina HiSeq 2500 at the JHU GRCF High Throughput Sequencing Center.
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bioRxiv - Microbiology 2019Quote: ... the TUNEL assay was performed with TUNEL Apoptosis Assay Kit (Roche). Briefly ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and libraries prepared using the KAPA mRNA HyperPrep Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Sequencing libraries were quantified using qPCR Library Quantification kits (Roche, #KK4824).
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bioRxiv - Cell Biology 2021Quote: ... and reverse transcribed into RNA using the DIG labelling kit (Roche) (SP6 enzyme for antisense probe and T7 for sense probe) ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were generated with the Kappa HyperPrep kit (Roche #07962363001) and submitted for single-end Illumina sequencing.
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bioRxiv - Physiology 2021Quote: ... and cDNA prepared using Transcriptor High Fidelity cDNA Synthesis Kit (Roche). Relative abundance of cDNAs was determined a Roche LightCycler 480 ...
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bioRxiv - Microbiology 2021Quote: ... and quantified using the Kapa Quantification Kit for Illumina Sequencing (Roche). Amplified libraries were pooled at equal molar amounts and sequenced on a NextSeq (Illumina ...