Labshake search
Citations for Roche :
1401 - 1450 of 6198 citations for Mouse Serine threonine Protein Kinase 17B STK17B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... 1 mM ATP (+ 2 mM DTT for Strep-KaiC36803 proteins)] including protease inhibitors (protease inhibitor cocktail, Roche or Nacalai). Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell lysates - non-transfected or overexpressing EGFP-tagged proteins -were prepared in lysis buffer (20mM HEPES, 1mM EDTA, 0.2% Triton X-100 and protease inhibitor cocktail (Roche)) briefly sonicated (three times 5 second pulses with 30 seconds rest ...
-
bioRxiv - Epidemiology 2019Quote: ... and C-reactive protein (CRP) was measured by automated particle-enhanced immunoturbidimetric assay (Roche UK, Welwyn Garden City, UK). CRP levels were measured at nine ...
-
bioRxiv - Cell Biology 2019Quote: ... Samples were boiled 5 min and 50 μg of proteins were separated by electrophoresis on 12.5% SDS-PAGE and subjected to immunoanalysis with either anti-GFP antibody (Roche), anti-Pgk1 antibody (Invitrogen) ...
-
bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
-
bioRxiv - Microbiology 2021Quote: ... 200 μg each lysate was pre-cleared with 20 μl of Protein A agarose beads (3 mg/ml, Roche) in 200 μl for 1 hour at 4°C on a Labquake Rotator (Barnstead Thermolyne) ...
-
bioRxiv - Microbiology 2021Quote: ... C-reactive protein and D-dimer levels were measured using Cobas Integra 400 plus analyzer (Roche Diagnostics Nederland B.V.).
-
bioRxiv - Cell Biology 2021Quote: The protein contents were extracted utilizing an RIPA lysis buffer (Beyotime, Shanghai, China) and protease inhibitor (Roche, Basel, Switzerland). Next ...
-
bioRxiv - Cell Biology 2021Quote: Protein from 2D hPSC-cardiac cell cultures was extracted using RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche). Protein lysate concentration was estimated using a BCA assay (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein lysate was electrophoresed into 6-10% SDS-PAGE gels and transferred to a PVDF membrane (Roche, 03010040001). After blocking with 5% BSA ...
-
bioRxiv - Genomics 2020Quote: ... Protein lysates were prepared by manually homogenizing frozen tissue in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Proteins were separated on a 4-20% TGX SDS-PAGE gel (Bio-Rad ...
-
bioRxiv - Pathology 2021Quote: ... 10 mM Na4P2O7) in the presence of protease inhibitors (completeTM, Mini, EDTA-free Protein inhibitor cocktail tablets, Roche, Sigma). Gut tissue and GDE were homogenized in the lysis buffer and sonicated three time for 30 seconds each at 10 μm amplitude ...
-
bioRxiv - Neuroscience 2020Quote: Proteins were extracted using Tris-buffered Saline (TBS) with 0.5% NP40 protein extraction buffer containing protease and phosphatase inhibitors (Roche), on ice ...
-
bioRxiv - Biochemistry 2019Quote: ... CaCl2 was added to a final concentration of 1.25 mM and incubated with 200 μl Anti-Protein C Affinity Matrix (Roche) for 4 hrs while rotating at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... Whole cell extracts were prepared after transfection and incubated with indicated antibodies together with Protein A/G beads (Roche) overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were harvested and ruptured with a Dounce homogenizer in the buffer A containing cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
-
bioRxiv - Biochemistry 2022Quote: ... The cell lysate was diluted to 0.8 mg/ml total protein concentration and 0.47% NP40 using dilution buffer (PBS plus protease inhibitor cocktail (Roche 04693159001) and precleared using Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1 magnetic beads for 2-4 hours at 4°C during gentle rotation ...
-
bioRxiv - Biochemistry 2022Quote: ... EDTA-free TCA buffer was used where phosphorylation of proteins was to be investigated by alkaline phosphatase-treatment (Roche) or Phos-tagTM (NARD Institute ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
-
bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
-
bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
-
bioRxiv - Cell Biology 2024Quote: ... Quantitative Western blot analysis was employed to determine concentration of recombinant protein using a recombinant GFP standard (Roche, 11814524001) with a defined concentration of 1 mg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... Whole cellular protein was extracted with RIPA buffer (Serva electrophoresis) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche). Western blots were performed following routine protocols55 ...
-
bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
-
bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
-
bioRxiv - Cancer Biology 2023Quote: Protein were isolated by using RIPA buffer with protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche, Mannheim, Germany, 04693116001), and concentrations were measured using the Qubit Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
-
bioRxiv - Pathology 2023Quote: ... Approximately 30-50 μg of proteins were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (Roche). The membrane was blocked with 5% non-fat milk in PBS for 1 h at room temperature and then incubated with the specific primary antibodies at 4°C overnight ...
-
bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
-
bioRxiv - Plant Biology 2024Quote: ... The eluted proteins were separated on 8% SDS-PAGE gel (37.5:1 acrylamide:bis-acrylamide) and probed with anti-HA (Roche, 3F10), anti-GFP (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... The ATP concentration of each sample was measured using a bioluminescence assay kit (ATP Bioluminescence Assay kit HTS II; Roche, Indianapolis, IN). Light emitted as a result of the reaction between D-luciferin and luciferase was detected using a 96 well micro-injector plate reader (POLARstar OPTIMA ...
-
bioRxiv - Genomics 2019Quote: ... using the HS NGS Fragment kit (DNF-474-33) and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). Each library was sequenced on half a lane of a rapid v2 flow cell (Illumina ...
-
bioRxiv - Genomics 2019Quote: ... using the HS NGS Fragment kit (DNF-474-33) and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). The library was sequenced on one lane of a rapid v2 flow cell (Illumina ...
-
bioRxiv - Developmental Biology 2019Quote: Non-radioactive RNA in situ hybridization was performed using a digoxigenin (DIG) RNA labeling kit and PCR DIG probe synthesis kit (Roche; http://www.rochediagnostics.us) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: A total of 100 ng of multiplex PCR amplicons were subjected to library preparation and dual-indexing using a KAPA HyperPrep Kit and a Unique Dual-Indexed Adapter Kit (Roche Applied Science) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The purified indexed PCR library products were quantified using the KAPA Library Quantification Kit from Roche (cat. no. 07960255001, kit code KK4844), normalized to a concentration of 7 nM ...
-
bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
-
bioRxiv - Cell Biology 2021Quote: ... apoptotic cells in pancreas frozen sections were detected by the TUNEL (TdT mediated dUTP nick end labeling) assay using a fluorescein-based detection kit (in situ death detection kit, Roche, Indianapolis, IN) according to manufacturer’s instructions ...
-
Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... Final library concentrations were quantified with both Qubit fluorometric quantification (DNA dsDNA HS kit, Thermo, #Q32851) and the KAPA Library Quantification kit (Kapa Biosystems, #KK4873). The samples were run on an Agilent 2100 Bioanalyzer (Agilent #G2939BA ...
-
bioRxiv - Immunology 2020Quote: ... and a quantitative Real-Time PCR was performed according to the manufacturer’s instructions (MMLV Kit, Life Technologies and Smart SYBRGreen kit, Roche Applied Science). Circadian gene expression was investigated using specific primers targeting Bmal1 and Clock genes (8) ...
-
bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
-
bioRxiv - Genomics 2022Quote: Whole genome library preparation (without duplex) Next generation sequencing libraries were generated using a double-stranded preparation kit (Kapa Hyper Prep Kit, Roche, cat#KK8502). Full-length adapters (IDT TruSeQ UDI plate ...
-
bioRxiv - Physiology 2023Quote: ... libraries were verified on a Fragment Analyzer using the High Sensitivity NGS kit (#DNF-474-0500) and quantified using the KAPA Library quantification kit (Roche, ref. KK4824).
-
bioRxiv - Immunology 2023Quote: ... Library yields were assessed by qPCR using the KAPA Library Quantification Kit (Complete kit, Universal) (F. Hoffmann-La Roche AG, Basel, Switzerland) on the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories Inc ...
-
bioRxiv - Molecular Biology 2020Quote: ... Signals were detected using anti-mouse secondary antibodies conjugated with alkaline phosphatase using BCIP/NBT substrates (Roche Biochemical, Mannheim). Similarly ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...