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Citations for Roche :
1551 - 1600 of 6508 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: HEK293T cells transfected with N-terminally sfGFP-tagged indicated variants of TAOK1 were lysed with HKT buffer (25mM HEPES pH7.2, 150mM KCl, 1% Triton X-100, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, cOmplete). Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed in Western Blot (WB) lysis buffer (1% SDS, 10 mM Tris, 1 mM EDTA, phosphatase and protease inhibitors (Roche, 04906837001 and 04693159001 ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 850 μL RIPA buffer (1×PBS, 1% IGEPAL, 0.5% Sodium Deoxy-cholate, 0.1% SDS, Roche Protease Inhibitor Cocktail) and added to the beads ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor, 1X Roche phosphatase inhibitor) followed by a 30’incubation on ice ...
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bioRxiv - Neuroscience 2023Quote: Cell pellets were resuspended in a lysis solution composed by 1 X Phosphate Saline Solution (PBS) supplemented with 1 X Complete Protease Inhibitor Cocktail (Roche) and then sonicated with a BioRuptor UCD-200 (Diagenode ...
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bioRxiv - Plant Biology 2023Quote: ... 1% [v/v] Triton X-100, 150 mM NaCl, 1 mM EDTA, 10% [v/v] glycerol, and 1× protease inhibitor cocktail [Roche]) by rotating at 4 °C for 1 h ...
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... Cell fraction was washed twice and resuspended into 30 μL with 1 × phosphate-buffered saline (PBS) and 1 × cOmplete proteinase inhibitor (Roche). Supernatant fraction was supplemented with 1 × cOmplete and reduced to 30 μL with vacuum centrifuge ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 1x Roche cOmplete Protease inhibitors), 1x 1 ml LiCL buffer (250 mM LiCl ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 1x Roche cOmplete Protease inhibitors) in a total volume of 900 μl ...
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bioRxiv - Physiology 2022Quote: ... the sections were blocked in TS7.5 containing 1% Blocking Reagent for 1 hr and then incubated with alkaline phosphatase-conjugated anti-digoxigenin sheep antibody (1:1000; 11093274910, Roche) and anti-EP3R rabbit antibody in this blocking solution overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 20% glycerol, 1% Triton X-100, and 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.5, 150 mM NaCl, 5% glycerol, 1% Triton X-100, 1□mM EDTA, 1X Complete Mini protease inhibitor cocktail [Roche]). Tissues were disrupted using a mechanical homogenizer (IKA T10 basic ...
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bioRxiv - Plant Biology 2022Quote: ... 100mM KCl, 10mM MgCl2, 1 mM PMSF, 5 mM Na3VO4, 5 mM NaF, 1 mM TCEP, cOmplete Protease Inhibitor Cocktail, Roche) in 2 steps ...
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bioRxiv - Microbiology 2022Quote: ... The total qPCR reaction volume was 1.5 □μL with 0.5□ μl of DNA (40□ng□μl-1) and 1□μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5□μM of PCR primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody incubation was performed for 16 h at 4°C in AB buffer supplemented with 1% v/v Fetal Calf Serum and anti-DIG antibody coupled to alkaline phosphatase (1∶5000 dilution; Roche). Sections were then washed thoroughly in AB and equilibrated in alkaline phosphatase buffer (AP - 0.1 M Tris–HCl pH ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail; Roche PhosStop tablet) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM CDTA pH 8.0, 0.5% Triton-X-100 supplemented with 1 mM ATP, 1 mM PMSF, and Roche protease inhibitors) for 75 mins on a nutating rocker ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were washed once then resuspended in a 1:1 ratio with CHAPS lysis buffer + protease inhibitors (Roche Complete) and frozen dropwise in liquid nitrogen before freezer-mill lysis ...
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bioRxiv - Plant Biology 2024Quote: ... 100 mM NaCl, 80 mM KCl, 1% glycerol, 0.1 % Triton, 10 mM DTT, plus 1 mini protease inhibitor cocktail tablet, Roche, Switzerland) per 20 ml of extraction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10% glycerol v/v) supplemented with 1 mM DTT and 1 x cOmplete EDTA-free protease inhibitor cocktail (Roche). Lysates were then clarified by centrifugation at 14000g for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: ... were lysed in deionized water containing 0.1 µg ml-1 RNase A (Roth, Karlsruhe, Germany) and 0.1 µg ml-1 DNase I (Roche, Mannheim, Germany), respectively ...
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bioRxiv - Microbiology 2024Quote: ... The heat-denatured RNAs were separated on a 1% agarose gel (95% 1× MOPS buffer and 5% formaldehyde) and transferred to a positively-charged nylon membrane (Roche) by capillary flow ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were washed twice with 1 mL ice-cold RIPA buffer supplemented with 1 mM DTT) and 1X PIC (Roche); inverted 10X and collected at 2000 x g for 2 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked for 2 hours at room temperature in 1% blocking solution (1% Western Blocking Reagent (WESTBL-RO, Roche) in TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was performed in duplicate using 1:20 or 1:5 dilutions of the cDNA and SYBR Green I master mix (Roche) with a LightCycler 480 II (Roche ...
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bioRxiv - Genomics 2024Quote: ... nuclei were isolated by resuspending lysed cells in complete LB2 (10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 × Roche cOmplete Mini) and incubating on a rotating wheel for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Plant Biology 2024Quote: ... 1.2 mM EDTA pH 8.0, 1.1% triton X-100, 1 mM DTT, 0.1mM PMSF, 1% Roche protease inhibitor cocktail tablet). This diluted supernatant was then incubated overnight with a specific antibody at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by blocking with TBST with 3% BSA and overnight incubation at 4°C on a platform rocker with an HRP-conjugated anti-HA antibody in TBST with 1 % BSA (1:5000, Roche). Membranes were then washed 3 times with TBST and detected using the SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo ...
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bioRxiv - Biophysics 2024Quote: MEF proteins were extracted with TNE lysis buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% NP40, protease inhibitor (Complete Mini; Roche), 20 mM NaF ...
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Ion channel function of polycystin-2/polycystin-1 heteromer revealed by structure-guided mutagenesisbioRxiv - Biophysics 2024Quote: ... Oocytes were subsequently transferred into 1 mL lysis buffer (Tris 25mM, NaCl 150 mM, EDTA 1mM, NP40 1%, Glycerol 5%, supplemented with Roche cOmplete™ protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2024Quote: Cells were resuspended in cold lysis buffer (50 mM Hepes pH 8, 50 mM NaCl, 5 mM MgCl2 and 1 tablet 50 mL−1 EDTA-free protease inhibitor, Roche) plus 0.5 mg/mL lysozyme and 0.1 mg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 300 mM NaCl, 1 mM DTT, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Cell Biology 2024Quote: ... 500 mg of fly heads from each genotype was homogenized in 2.9 ml of lysis buffer (8 M urea, 1% SDS, 1× PBS, 50 mM N-ethylmaleimide from Sigma, and a protease inhibitor cocktail from Roche). After the centrifugation of lysates at 16,000g at 4 ºC for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed in PBS and resuspended in ice cold lysis buffer (PBS supplemented with 50 mM NaCl, 1% Triton X-100, and 1× Complete EDTA-free protease inhibitor cocktail (Roche), incubated on ice for 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 x phosphatase inhibitor cocktail (PhosSTOP, Roche). Total protein concentration was determined with Bradford protein assay kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 μg ml−1 of DNase (Roche, #10104159001), 50 μg ml−1 of RNase (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Mouse monoclonal to GFP (Roche #11814460001, 1:2000), Rabbit monoclonal to LRRK2 phospho-S1292 (Abcam #ab203181 ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2020Quote: ... rhodamine α-DIG Fab fragments (Roche 1:20) followed by Texas Red α-sheep antibody (Vector Labs ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-GFP (1:200 dilution; Roche, 11814460001) was used for staining Cse4-GFP and rat anti-HA (1:200 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were blocked with 1% blocking reagent (Roche) for 1 h at RT ...
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bioRxiv - Genetics 2020Quote: ... 1 mM EDTA and proteinase inhibitor (cOmplete, Roche) for 30 min at 4 °C on a rocking platform followed by centrifugation at 15,000 rpm for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mg/ml yeast tRNA (Cat. # 10109509001, Roche), 1×Denhardt’s [1% (w/v ...
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bioRxiv - Developmental Biology 2020Quote: ... 1× complete protease inhibitor cocktail (Roche, Lot # 3024150)] ...
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bioRxiv - Biophysics 2022Quote: ... 1 cOmplete EDTA free protease inhibitor tablet (Roche) per 50 ml Buffer L ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with collagenase (Roche, 11088793001, 1 mg/mL) and hyaluronidase (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mM EGTA and protease inhibitors (Roche Diagnostics). For homogenization ...