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Citations for Roche :
1451 - 1500 of 1954 citations for Centromere Protein O CENPO Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the pellet washed in 1x PBS then re-suspended in 1x protein loading buffer (4x NuPAGE® LDS sample buffer [Invitrogen], 1x PBS and 25 uL ß-mercaptoethanol, 2x Roche cOmplete Mini protease inhibitor cocktail tablets ...
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bioRxiv - Neuroscience 2020Quote: Cells from mouse brain or spleen tissues were lysed with T-PER Tissue Protein Extraction Reagent (Thermo) containing Complete Protease Inhibitor Cocktail (Roche Applied Science). Protein concentrations were determined with BCA protein assay reagents (Pierce) ...
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bioRxiv - Immunology 2022Quote: Anti-SARS-CoV-2 (N protein) IgM/IgG levels in the serum were measured using an Elecsys Anti-SARS-CoV-2 with cobas8000 (Roche Diagnostics KK) at the Department of Clinical Laboratory ...
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bioRxiv - Microbiology 2022Quote: ... The gene encoding this protein was amplified from genomic DNA of the SECphi4 phage using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601) with primers AS330+AS331 (Table S6) ...
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bioRxiv - Cancer Biology 2022Quote: Total protein cell extracts were obtained by lysing the cells with RIPA buffer supplemented with 1x protease inhibitors (PI) (Roche, Milan, Italy). Proteins were quantified using the BCA method (Pierce ...
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bioRxiv - Cancer Biology 2022Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were prepared using T-PER (Pierce Chemical Co., IL) in the presence of protease inhibitors (Roche Applied Science, Barcelona, Spain), and subjected to Western blot analyses with anti-rat anti-ABCA1 antiserum (kindly donated by Dr ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates of 20 μg protein were separated in a SDS-12% polyacrylamide gel (PAGE) followed by transferring proteins to a polyvinylidene difluoride (PVDF) membrane (Roche, catalog 3110040). After blocking in 5% milk (in 1xphosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2022Quote: Mouse liver tissues and cells were homogenized and lysed in Protein Extraction Reagent (P0013, Beyotime Biotechnology) supplemented with protease inhibitor cocktail (04693159001, Roche, Basel, Switzerland). Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 HUVEC were washed with CUT&RUN wash buffer (20 mM HEPES pH 7.9, 150 mM NaCl, 500 nM spermidine, 1X Roche Protein Inhibitor Cocktail) at RT ...
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bioRxiv - Microbiology 2024Quote: ... respectively) was split in two samples with similar protein concentration of which one was mixed with Complete Protease Inhibitor (Roche, Mannheim, Germany) and the other one with ddH2O as a control ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect electroporation-competent cells ...
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bioRxiv - Plant Biology 2021Quote: ... total protein was isolated from Agrobacterium infiltrated leaves using extraction buffer (50mM Tris-Cl, 1% β-Mercaptoethanol and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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bioRxiv - Biochemistry 2020Quote: ... the expression medium containing the secreted fusion protein was supplemented with 1/4 tablet of protease inhibitor (complete EDTA-free protease inhibitor cocktail tablets, Roche Diagnostic GmbH, 45148300) and transferred to a 15 ml Falcon tube containing 200 μl of washed ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg of proteins from the microsomal membrane pellet (P13) were incubated with 30 μg/ml of proteinase K (Roche Diagnostics, Mannheim, Germany) for 30 min on ice ...
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bioRxiv - Developmental Biology 2022Quote: Testes from adult mice were cut into ~20 mg pieces and transferred into ~200 μl pre-cooled denaturing buffer from the Minute Total Protein Extraction Kit (SD-001, Invent Biotech, Plymouth, MN, USA) containing protease inhibitors (CO-RO, Roche, Indianapolis, IN, USA) and phosphatase inhibitors (PHOSS-RO ...
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bioRxiv - Cell Biology 2023Quote: ... were used to remove salts from purified protein and to exchange to freezing buffer (20mM HEPES, 100mM NaCl, 0.5mM EDTA, TCEP 0.5mM and Roche cOmplete™ Protease Inhibitor Cocktail), using the gravity protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The elute was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect TG1 cells ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue extracts were subjected to TCA precipitation as previously described53.Washed protein pellets were solubilized in 900 μl of urea buffer (8M urea, 150mM NaCl, 50mM Tris pH8, 1X Roche complete protease inhibitor). Protein quantitation was performed using Qubit fluorometry (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-peroxidase antibodies (1:500, Roche) and revealed using the TSA-Plus Cyanine-3 kit (Perkin-Elmer) ...
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bioRxiv - Developmental Biology 2020Quote: ... and sequential incubation with an anti-GFP primary antibody (mouse anti-GFP, 1:200, 11814460001, Roche) and a corresponding secondary antibody (goat anti-mouse Alexa Fluor 488) ...
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bioRxiv - Neuroscience 2019Quote: ... the DIG-labeled probe was visualized by using alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics) and Fast Red (Roche Diagnostics) ...
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bioRxiv - Physiology 2019Quote: ... For Western blots and immunoprecipitation experiments the following primary antibodies were used: anti-HA (Roche, 11867423001), anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with anti-HA-peroxidase antibody (1:5,000; Roche, Cat# 12013819001, clone 3F10) for 1 h at room temperature followed by washing with PBS-T for three times ...
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bioRxiv - Plant Biology 2019Quote: ... Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001, Roche) or anti-actin 1:2,000 (A0480 ...
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bioRxiv - Cell Biology 2019Quote: ... Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche), anti-cMYC antibody 9E10 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... HA-Kir6.2 was detected with HRP-conjugated rat anti-HA antibody (Roche, 3F10, 0.005 μg/ml). GFP was detected with mouse anti-GFP antibodies (AbCam ...
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bioRxiv - Evolutionary Biology 2021Quote: ... labelled riboprobes were detected using anti-digoxigenin or anti-fluorescein antibodies conjugated to horseradish peroxidase (Roche). Fluorescent tagging was performed using Alexa Fluor 594 and 488 dyes with streptavidin Tyramide Signal Amplification (TSA ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with 1:2000 dilution of anti-DIG-alkaline phosphatase-coupled antibody (Roche, Switzerland) in 10% sheep serum overnight ...
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bioRxiv - Biophysics 2021Quote: ... The following primary antibodies were used at the indicated dilutions: mouse anti-Myc (Roche, 1:3000), goat anti-p75NTR (Neuromics ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibody (1:1000, 11093274910; Roche) at 4°C for two days ...
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bioRxiv - Neuroscience 2021Quote: ... Post hoc immunostaining was performed using rat anti-HA primary antibodies (Roche, #11 867 423 001) and anti-rat Alexa568 (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used in this study: mouse monoclonal anti-GFP (1:2’000; Roche), anti-HA (clone 16B12 ...
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bioRxiv - Neuroscience 2019Quote: ... Dioxygenin (DIG)-labeled probes were detected using peroxidase-conjugated anti-DIG antibodies (Roche Diagnostics, Mannheim, Germany). Peroxidase activity was detected using fluorescein-tyramide conjugate ...
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bioRxiv - Cell Biology 2021Quote: Commercially available primary antibodies used for western blot are rat anti-HA tag (clone 3F10; Roche), rabbit anti-Cyclin B1 (4138S ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used in this study were: rat anti-HA (1:250; Roche 3F10, RRID: AB_2314622), rabbit anti-cleaved Caspase-3 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Dilutions for primary antibodies were: mouse monoclonal (mixture of clones 7.1 and 13.1) anti-GFP (Roche), 1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... the slides were incubated for 5 h at 8°C with mouse anti-DIG antibody (Roche, Basel ...
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bioRxiv - Microbiology 2019Quote: ... GRA5 blots were subsequently incubated with an anti-mouse secondary horseradish peroxidase (HRP)-conjugated antibody (Roche). The GRA7 blot was incubated with a secondary antibody (HRP)-conjugated anti-rabbit antibody (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... Dig was detected using anti-Dig antibody conjugated with alkali phosphatase and CDP-Star substrate (Roche) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the southern blot was detected by using an anti-digoxygenin antibody coupled to alkaline phosphatase (Roche) and the labeling and detection starter kit (Roche).
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bioRxiv - Developmental Biology 2022Quote: The following primary antibodies were used in this study: anti-HA (rat, 1:200, 11867423001, Roche), anti-V5 (mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analysed by western blotting using a α-GFP antibody (Roche, 11 814 460 001), and a α-Pgk1 antibody (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated for 1h in 1% milk prepared in TBST with anti-GFP antibody (Roche). The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad) ...