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Citations for Roche :
1301 - 1350 of 1954 citations for Centromere Protein O CENPO Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The hybridization signal was detected by horseradish peroxidase (HRP)-conjugated anti-fluorescein antibody (Roche) with TSA-fluorescein ...
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bioRxiv - Pathology 2023Quote: ... The following antibodies were used: rat anti-HA (3F10, 1:400; Roche, Switzer land), mouse anti-Chaoptin (24B10 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:5000; Roche) for 90 minutes at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... rabbit anti-furin antibody (1:800, 18413-1-AP, proteintech) and DAPI (10236276001, Roche). The bright-field and immunofluorescent images were acquired using a Pannoramic MIDI system (3DHISTECH ...
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bioRxiv - Biochemistry 2023Quote: ... Monoclonal mouse anti-GFP antibodies were used (Cat#11814460001, clones 7.1 and 13.1, Roche) at 1:1,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... transferred to PVDF membrane and inmunodecorated with antibodies against: anti-HA (Roche Applied Science), anti-Cytb (García-Guerrero et al. ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in the study: rat anti-HA (Roche, Basel, Switzerland), rabbit anti-HA (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated with 1:5,000 dilution of anti-digoxigenin-AP conjugated antibody (Roche) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then stripped and incubated with rat anti-HA primary antibody (Roche) at 1/2000 dilution in PBST 5% milk and washed 3 times ...
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bioRxiv - Biochemistry 2023Quote: ... the blots were probed with HRP-conjugated rat anti-HA antibody (clone 3F10, Roche) at a 1:1000 dilution and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated with anti-DIG-AP Fab fragments antibody (Roche, 11093274910, 1:2000) for overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Flow cells were incubated with 1 μl of α-digoxigenin antibody (Roche, 150 U) diluted in 30 μl TB buffer (40 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase (Roche 11093274910). The zebrafish antisense RNA probes used in this study include myl7 ...
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bioRxiv - Biochemistry 2023Quote: ... as secondary antibodies and detection with the Lumi-Light Plus Western Blotting Substrate (Roche). Images were acquired and quantitated with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated in alkaline phosphatase-labeled anti-DIG antibody (Roche; 1:600) in TNB for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated overnight with primary antibodies: rat monoclonal anti-HA (1:1000, ROCHE), or Actin5c (1:200 ...
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bioRxiv - Genetics 2024Quote: ... The incorporated BrdU was then detected using a FITC-labeled anti-BrdU antibody (Roche). Coverslips were mounted with Prolong Gold antifade plus DAPI (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The hybridization result was detected with anti-DIG-AP antibody (1:2000, Roche, 11093274910) and NBT/BCIP (1:500 ...
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bioRxiv - Plant Biology 2024Quote: ... and then detected with anti-digoxigenin antibody conjugated to alkaline phosphatase (Roche Diagnostics GmbH) and Western Blue stabilised substrate (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies: anti-HA-HRP (REF-12013819001, Clone 3F10, Roche/Millipore Sigma, St. Louis, MO), anti-FLAG (F3165 ...
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bioRxiv - Microbiology 2024Quote: ... The respective antibodies are coupled to AP and CDP-Star chemiluminescent substrate (Roche, Switzerland) was used to generate a luminescent signal ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies used were anti-c-myc (11667149001, 9E10 mAb from Roche; 1:250), anti-α-Tubulin (T5168 Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Detection of mRNA was performed with anti-DIG-alkaline-phosphatase-conjugated antibody (Roche Diagnostics) and the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Genomics 2024Quote: ... and incubated overnight at 4°C with Anti-Dig-AP antibody (Roche, 1:5000) in 1% lamb serum ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples (containing 20 μg of total protein) were mixed with loading buffer and separated on 12 % SDS-PAGE gels and transferred to PVDF membranes (Roche, Switzerland). After blocking with Tris-buffered saline with 0.1% Tween 20 and 5 % nonfat dry milk ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting yeast extracts were used for immunoblot analysis using anti-Myc (MBL, Woburn, MA, United States) for bait proteins and anti-HA (Roche, Switzerland) for prey proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysate of 10-20 μg was run on 10–15% SDS-PAGE gel and transferred toa PVDF membrane (Roche, USA). The membrane was incubated for 60 min at room temperature in 5% BSA solution ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2020Quote: Western blotting was performed as previously reported.[26] Protein extracts were prepared using RIPA buffer containing EDTA protease (Roche Applied Science) and phosphatase inhibitors (Phostop Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... mice and lysed on ice for 30 min in protein lysis buffer (1 mM Na3VO4, 1 mM NaF, complete protease inhibitor cocktail (Roche, USA), 1% Triton X-100 in PBS) ...
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bioRxiv - Plant Biology 2020Quote: ... Resulting yeast extracts were used for western blot analysis using anti-Myc HRP-DirecT (MBL, Woburn, MA, United States) for bait proteins and anti-HA (3F10)-HRP (Roche, Switzerland) for prey proteins.
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bioRxiv - Immunology 2021Quote: Secreted twin strep-tagged gp140-soc proteins in the harvested and filtered supernatant (1L) were supplemented with protease inhibitor tablets (Roche Diagnostics) to prevent protein degradation and 5 ml of BioLock biotin blocking solution (iba Life Sciences GmbH ...
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bioRxiv - Systems Biology 2019Quote: ... The cross-linked proteins-RNA were cut directly from the gel and incubated with 160 μg of Proteinase K (Roche, 3115801001) in 600 μl wash buffer 2 supplemented with 1% SDS and 5 mM EDTA at 55°C for 2-3 hours with mixing ...
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bioRxiv - Neuroscience 2020Quote: Total proteins from mouse cerebella or human cerebellar cortex were obtained by lysis in RIPA buffer containing protease inhibitors (Complete, Roche Diagnostics), followed by sonication and centrifugation using an established protocol in the laboratory31 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were extracted from RAW264 using radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Complete Mini, Roche Diagnostics GmbH, Mannheim, Germany) and phosphatase inhibitors (PhosSTOP EASYPack ...
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bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated protein was incubated with 50ug fresh mitochondria extraction in import buffer containing ATP regeneration system (Creatine kinase (Roche 10127566001), Creatine Phosphate (Sigma-Aldrich 10621714001 ...
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bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 10 μg total proteins were separated on a 12% SDS-PAGE gel and transferred to the PVDF membranes (Roche, CA, USA) using an electro-transfer apparatus (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Hand dissected ovaries from 100-150 flies (depending on the expressed protein) of appropriate genotypes were lysed in RIPA buffer supplemented with Complete Protease Inhibitor cocktail (Roche, 11836170001) and 20 mM NEM ...
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bioRxiv - Neuroscience 2023Quote: Proteins were detected by Western blot analysis after separating 30–50 μg of total protein lysate prepared in RIPA buffer with protease inhibitor cocktail (cOmplete™, Roche) on a 4– 15% Mini-PROTEAN® TGX™ Precast Gel (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein lysates were prepared in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor mixture (Roche Applied Science, IN, USA) and 2mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251) ...
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bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
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bioRxiv - Molecular Biology 2023Quote: ... containing either single (for the IgG screen) or serial dilutions (for full IgG multi-point kinetics) of HEL protein (Roche #10837059001). For measurement of Fab binding kinetics ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...