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Citations for Roche :
1451 - 1500 of 1756 citations for 3 methy 5 isobutylhydantion since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 mL ice-cold lysis buffer (100 mM NaCl, 5% glycerol, 1 mM dithiothreitol and protease inhibitor cocktail (cOmplete, EDTA-free; Roche) in 50 mM Tris HCl ...
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bioRxiv - Neuroscience 2019Quote: ... cells were lysed in Hepes buffer (20 mM Hepes, 1% CHAPS, 100 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail III (Sigma)) ...
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bioRxiv - Developmental Biology 2019Quote: Total DNA was isolated from L3 larvae or 5-days old adults of Iduna homozygous mutants and control sequencing strain using the Roche genomic DNA extraction kit (Roche). To confirm the mutant line ...
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bioRxiv - Biochemistry 2019Quote: ... were lysed with lysis buffer (25mM Tris, 150mM NaCl, 1mM EDTA, 1% NP-40, 5% Glyceral) with proteinase inhibitor cocktail (Roche) on ice for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 24 hours in the presence of SARS-CoV-2 specific MPs [1 μg/mL] or 5 μg/mL phytohemagglutinin (PHA, Roche) in 96-wells U-bottom plates at 1×106 PBMCs per well ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (10 mg/kg for the first dose with each subsequent dose at 5 mg/kg; atezolizumab, Roche), anti-TGF-β (10 mg/kg ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were pelleted at 1350xg for 5 min at 4°C and lysed in 5 mL LB2 (10 mM Tris-Cl pH 8.0, 5 M, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 mM PMSF, Roche protease inhibitors) for 10 min at RT with rotation ...
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bioRxiv - Genomics 2020Quote: ... was synthesised commercially and then labelled with digoxigenin-11-2’-deoxyuridine-5’-triphosphate (DIG-11-dUTP) using terminal transferase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were added (0.5 g to each 100 mg pellet) along with protease inhibitor solution (complete mini EDTA-free protease inhibitor cocktail, Roche), dH2O and 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Genomics 2020Quote: ... They were then incubated with TNB (5% FBS in TNT) for 15 min before incubation with peroxidase-conjugated anti-DIG antibody (DIG-POD, Roche) 1:200 in TNB for 2 hr at RT ...
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bioRxiv - Immunology 2020Quote: The reaction mix per sample for quantitative real-rime PCR (qPCR) contained: 5 µl of the LightCycler 480 SYBR Green I Master Mix (04707516001, Roche), 2.5 µl cDNA and 2.5 µl primers in a total reaction volume of 10 ul ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA quantification by real-time PCR was performed on a 20-fold dilution of the ligation mixture with a QuantStudio 5 system using the LC480 SYBR Green Master mix (Roche) and primers hybridizing in the adapter and Ty1 LTR (Supplementary Table 3) ...
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bioRxiv - Microbiology 2020Quote: ... thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
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bioRxiv - Physiology 2020Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Microbiology 2019Quote: ... Each sample was run in triplicate using a 10 μl reaction volume consisting of: 5 μl of CyberGreen Mastermix (Roche), 0.5 μl forward primer (30 μM) ...
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bioRxiv - Immunology 2019Quote: A single-cell suspension of lung cells was obtained by mincing whole mouse lungs in RPMI and then digesting each lung in 5 mL of RPMI + 10% FCS with 1 mg/mL Collagenase A (Roche) and 2000 U/mL of DNAse I for 45 minutes in a shaking incubator at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Chromatin cross-linking was reversed at 65°C for 5 hours with 280 mM NaCl and 88 μg/mL RNase DNase free (11119915001, Roche). Proteins were then digested using 88 μg/mL of Proteinase K (03115828001 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cell Biology 2020Quote: ... anti-SPD-5 (1:1000, a generous gift from B. Bowerman, Hamill et al., 2002) and anti-GFP (1:500, Roche) overnight at 4°C and with secondary antibodies Alexa488 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5% glycerol with the addition of 1 EDTA-free cOmplete protease inhibitor cocktail tablet per 50 mL lysate (Roche). Cells were lysed by sonication on ice with stirring and the lysates were clarified by centrifugation at 20,000 g for 30 minutes at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: Pitx1 WISH were performed on 40-45 somite stage mouse embryos (E12.5) using a digoxigenin-labeled Pitx1 antisense riboprobe transcribed from a cloned Pitx1 probe (PCR DIG Probe Synthesis Kit, Roche), as previously described in (Kragesteen et al ...
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CRISPR-Csx28 forms a Cas13b-activated membrane pore required for robust CRISPR-Cas adaptive immunitybioRxiv - Biochemistry 2021Quote: ... The membrane fraction was resuspended in membrane resuspension buffer (50 mM sodium phosphate buffer, 300 mM NaCl, 5% glycerol, EDTA-free protease inhibitor (Roche), 2% DDM ...
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bioRxiv - Biochemistry 2021Quote: ... a linear DNA tether of the same length was amplified using a digoxigenin-modified primer instead of NTA (5’-DIG-NHS-TCCAAAGGTGAAGAACTGTTCACC, Integrated DNA Technologies, Inc. USA) to bind to anti-digoxigenin Fab fragment antibodies (11214667001, Roche).
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bioRxiv - Biochemistry 2021Quote: Cell extracts were prepared in 1% NP40 lysis buffer (20 mM Hepes pH 7.5, 150 mM NaCl, 1% NP40, 50 mM NaF, 1 mM Na3VO4, 10% glycerol, and protease inhibitor cocktail from Roche) at 4 °C for 15 mins ...
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bioRxiv - Immunology 2021Quote: ... was used for the preparation of sequencing libraries corresponding to surface marker and cytokine while additional part (5 µl) was used for the construction of sequencing library of sample hashtags using KAPA HiFi Master Mix (Roche). The 2nd-round P7/read 2-incorporated PCR primers used for protein library preparation were listed in Table S2 with the P5/read 1-incorporated primer the same to that used in cDNA library ...
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bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were pelleted by spinning at 500 g for 5 min at 4°C and resuspended in 1 mL cold CUT&RUN wash buffer (20 mM HEPES pH 7.5, 0.2% Tween-20, 150 mM NaCl, 0.1% BSA, 0.5 mM spermidine, 10 mM sodium butyrate, Roche Protease Inhibitor Cocktail). 10 μL concanavalin-coated bead slurry (Bangs Laboratories (Fishers ...
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bioRxiv - Microbiology 2021Quote: ... the bacterial cells were suspended in 20 ml cold buffer A (500mM NaCl, 50mM Tris pH 7.5, 5% glycerol, 10 mM imidazole) supplemented with cOmplete™ protease inhibitor cocktail (Roche), 100 ug/ml lysozyme ...
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bioRxiv - Neuroscience 2020Quote: ... digested using 0.1 % papain at 37°C for 10 min and for 5 additional minutes in presence of DNAse1 (1 mg/ml, Roche). After stopping papain activity by adding MC+ media ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...
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bioRxiv - Immunology 2021Quote: Muscularis was finely cut and digested in HBSS Mg2+Ca2+ + 5% FBS + 1x NaPyr + 25mM HEPES + 50 μg/ml DNaseI (Roche) + 400 U/ml Collagenase D (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Cells pellets were immediately snap frozen on dry ice for about 5 mins and then thawed and prepared for lysis using a Magnalyser (Roche) at 7000 rpm for 1 minute followed by cooling on ice for 4 min ...
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bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
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bioRxiv - Genomics 2021Quote: ... PCR products from the 5 reactions were mixed for library preparation using FastStart High Fidelity PCR System dNTPack (Roche, 3553400001) and Nextera XT Index Kit (illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were diluted 5 times in water before quantitiative real-based protocol time PCR on a LightCycler LC480 (Roche), using SYBR-based protocol based Mesa Blue qPCR Mastermix (Eurogentec ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM NaCl, 50 mM sodium pyrophosphate, 50 mM sodium fluoride, 1% Triton-X-100, 10% glycerol, 5 mM EDTA, Roche MiniProtease Inhibitor cocktail tablet ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and Supplementarylemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... N’-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Wells were then washed twice with PBT 0.2% and blocked with PBT 0.2% containing 5% bovine serum albumin (Roche, Cat# 10735094001). Primary antibody staining with anti-Wg (1:500 ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with SAE2-T2A-SAE1 cDNA in the pcDNA5/FRT/TO vector and the Flp-recombinase cDNA in the pOG44 vector at a 5:1 pcDNA5/FRT/TO DNA: pOGG44 DNA ratio using FuGene6 (Roche) at a ratio of 4:1 FuGene (μl) ...
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bioRxiv - Biochemistry 2022Quote: ... or octamer-mix were done at 900 MHz 1H Larmor frequency at 298 K in NMR buffer (25 mM NaPi, pH 7, 5% D2O, with 1x protease inhibitors (complete EDTA-free cocktail, Roche)) with 600 mM NaCl for the titrations with H2A-H2B and H3-H4 and 300 mM NaCl for titration with octamer-mix ...
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bioRxiv - Plant Biology 2022Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 μl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Molecular Biology 2022Quote: ... The loci of interest were first amplified with 15 cycles of PCR from 2 μL (∼100 ng) of genomic DNA eluate using a 5 μL Kapa HiFi HotStart polymerase reaction (Roche). The first PCR was then diluted with 25 μL of DNAse-free water ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... finely minced and incubated for 75 min at 37°C in RPMI supplemented with 5% penicillin-streptomycin and 0.4 mg/ml Liberase™ TM (Roche). Digest was stopped by adding an equal volume stopping solution of RPMI supplemented with 10% FCS to the digestion mixture and keeping cells on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...