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Citations for Roche :
1351 - 1400 of 1756 citations for 3 methy 5 isobutylhydantion since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol and 0.5 mM TCEP supplemented with 5 Units/mL Benzonase and an EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in an equal volume of lysis buffer (1M NaCl, 25mM Tris pH7,5, 5% Glycerol,1mM DTT, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mM Tris-HCl [pH 7.5], 150mM NaCl, 1 mM EDTA, 0.2% IGEPAL® CA-630, 0.5 mM DTT, 1X protease inhibitor cocktail [Roche]) in a 3:1 v/w ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... embryos were with PBT (x3, 15mins) and incubated in prehybridization buffer (50% deionized formamide, 5× SSC, pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were placed in 1.5mL microcentrifuge tubes containing 1mL of 5% cOmpleteTM EDTA-free protease inhibitor cocktail (PIC) (Roche Diagnostics Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... A probe binding the left flank of the acbd4/5 locus was generated using DIG probe labelling mix (Roche) as per the manufacturer’s instruction (primers acbd4/5_LF_fw and acbd4/5_LF_rv ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in cold Buffer A-5 (20mM Tris-HCl, pH 7.9, 500mM NaCl, 5mM Imidazole) with an EDTA-free protease inhibitor tablet (Roche) and kept on ice ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed using Transcriptor High Fidelity cDNA Synthesis Kit and a specific 3’-UTR DENV-1 primer (Roche Applied Science, Mannheim, Germany), d1a5B 5’-AGAACCTGTTGATTCAACRGC-3’ (62) ...
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bioRxiv - Microbiology 2022Quote: ... initial denaturation at 95°C for 3 min, followed by 40 cycles of amplification (95°C for 15 sec, 60°C for 30 sec) (Roche LightCycler 96, Roche Diagnostics). For absolute quantification ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 10 minutes at 37°C in a 3% (w/v) collagenase A solution in PBS (Collagenase A, Roche Diagnostics GmbH, Germany, 10103586001). Cells were recovered by centrifugation for 5 minutes at 300g prior cell counting on Malassez cells after Trypan blue staining ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Cancer Biology 2021Quote: ... at 95°C for 5 min (90°C for 15 sec, 60°C for 60 sec) × 50 cycles (Light/Cycler Nano, Roche). The primer sets used in this study were described in the supplemental Table 1.
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...
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bioRxiv - Biochemistry 2021Quote: ... 137.5 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM sodium fluoride, EDTA-free protease inhibitor cocktail [Roche]). 1 mM orthovanadate was added to the lysis buffer to prevent binding of substrates to the PTP1B trapping mutant ...
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bioRxiv - Cell Biology 2019Quote: ... and resuspended in Laemmli sample buffer (2% SDS, 5% β 50 mM Tris-Cl pH 6.8) supplemented with Protease Inhibitor Cocktail and PhosStop (Roche) for isolation of total cellular protein ...
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bioRxiv - Cell Biology 2019Quote: Zebrafish samples for Western blot analysis were deyolked and directly lysed in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 10 ul per embryo unless otherwise indicated ...
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bioRxiv - Cell Biology 2019Quote: ... HUVEC cells for Western blot analysis were lysed directly in 2x Laemmli Sample Buffer containing 5% b-ME and a PhosSTOP tablet (Roche), 500ul per T-25 culture flask.
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bioRxiv - Cell Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... Seminiferous tubules retained on the filter were collected and incubated at 32 °C for 25 min in GBSS containing 1.2 mg/mL of Collagenase Type I and 5 μg/mL DNase (Roche 11284932). Cell aggregates were sheared gently by 10 rounds of pipetting with a wide orifice plastic transfer pipet and filtered through the Cell strainer to remove cell clumps ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was divided into 10 μl reactions containing 5 μg of RNA and was treated for 30 mins with 10 units of DNAse I (#04716728001; Roche). Then DNAse I was inactivated by addition of 1 μl of 25 mM EDTA and incubation at 70°C for 10 min.
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bioRxiv - Developmental Biology 2021Quote: ... A T7 RNA polymerase binding site with short 5’-tail (aaaaTAATACGACTCACTATAG) was added to reverse primers for transcription using T7 RNA polymerase incorporating DIG labelled ribonucleotides (NEB, Roche). PCR amplified probe templates were confirmed by sanger sequencing.
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC ...
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bioRxiv - Neuroscience 2021Quote: ... and overlaid on top of 29% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 29% Iodixanol, 1x protease inhibitor (Roche, 11873580001), 0.4 U/ul RNAse inhibitor (Takara ...
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bioRxiv - Developmental Biology 2021Quote: The fourth and third mammary glands were dissected from 5.5 to 6.5-wk-old female mice, minced with scissors and digested in Digestion medium (DMEM/F12, 2mg/ml Collagenase A (Roche), 5μg/ml insulin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% Tween 20) for 5 min each and then incubated in the AP reaction buffer with NBT/BCIP substrate (Roche).
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bioRxiv - Developmental Biology 2020Quote: ... fixed embryos were homogenized in 2-5 ml freshly prepared lysis buffer (50 mM Tris pH7.5; 150 mM NaCl; 5 mM EDTA; 0.5 % NP-40; 1.15 % Triton X-100; 1x Roche Complete protease inhibitors) with a Dounce Homogenizer on ice ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... The PCR reaction contained 5 μl of 2X Kappa Hifi Hotstart ReadyMix (Kappa Biosystems, Roche Sequencing and Life Science, USA), 2 μl of 0.2 mg/ml BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... then harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES-KOH pH 7.0, 500 mM NaCl, 5 mM β-mercaptoethanol, 1x Protease Inhibitor Cocktail (Roche)) and lysed by sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation and the proteins in the supernatant were purified by gravity Ni-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Cell Biology 2019Quote: 5 dpf zebrafish larvae were killed on ice and eyes dissected in a solution of 5 mM NaCl with protease inhibitor cocktail tablets (Roche). Eyes were either snap frozen in liquid nitrogen and stored at -80°C or immediately lysed ...
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bioRxiv - Microbiology 2019Quote: ... RNA transcripts of the cloned 5’UTRB7 fragments were produced in vitro from the resulting purified PCR products by using the T7 Transcription kit (Roche) and labeled by using the RNA 3’ end biotinylation kit (Pierce) ...
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bioRxiv - Genomics 2019Quote: ... The supernatants from the digestion and initial decalcification step were purified using a 5 M guanidine-hydrochloride binding buffer with a High Pure Viral Nucleic Acid Large Volume kit (Roche). The extract was eluted in 100 μl of a 10mM tris-hydrochloride ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 2 mM DTT, 30 mM imidazole and complete EDTA-free protease inhibitors cocktail [Roche]) supplemented with 1 mM TCEP ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48-72 h and resuspended in lysis buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 30 mM imidazole, 1 mM TCEP and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed by sonication ...
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bioRxiv - Genomics 2019Quote: ... 5 ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
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bioRxiv - Immunology 2021Quote: Stimulated cells were lysed at 4 °C for 10 min in 1% NP-40 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche #11836170001), 1 mM Na3VO4 ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed at 4 °C for 10 min in 1% NP-40 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche# 11836170001), 1 mM Na3VO4 ...
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bioRxiv - Neuroscience 2020Quote: ... A single qPCR reaction is made of 5 μL 2xSYBR green mix (LightCycler® 480 SYBR Green I master, Roche), 2 μL H2O ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed using 2-5 times the volume of RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Roche), PMSF (1 mM ...