Labshake search
Citations for Roche :
1251 - 1300 of 1756 citations for 3 methy 5 isobutylhydantion since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The membranes were then blocked for an hour in 5% milk powder/PBS solution and probed with 1:2,500 rat anti-HA mAb 3F10 antibody (Roche) in 5% milk powder/PBS solution overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... PSH in DDM micelles or in POPC vesicles was diluted with MES-buffer (50 mM MES pH 6.0, 500 mM NaCl, 5 mM MgCl2, 1x PI mix complete (Roche)) to 1 - 2 µM PSH ...
-
bioRxiv - Biochemistry 2022Quote: ... pelleted cells were resuspended in lysis buffer (20 mM Tris-HCl pH 7.5, 500 mM NaCl, 5 mM MgCl2 with EDTA-free protease inhibitor (Roche)) ...
-
bioRxiv - Developmental Biology 2021Quote: ... resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) and incubated on ice for 10’ to isolate the cell nuclei ...
-
bioRxiv - Microbiology 2020Quote: ... 300 mM NaCl, 30 mM imidazole, 1 μM DNases, 0.75% CHAPS, 5[mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]), and lysed with high pressure cell homogenizer (Union-Biotech ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% milk and probed overnight at 4°C with 1:2,500 rat anti-HA mAb 3F10 (Roche). They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare) ...
-
bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
-
bioRxiv - Microbiology 2020Quote: ... Mycelia pellets were thawed on ice and resuspended in 0.4 mL ice-cold lysis buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Roche]) and sonicated (5x 15 sec on/15 sec off at 5-micron amplitude) ...
-
bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 1 mM EGTA, 10% sucrose, 0.8 M NaCl containing sarkosyl (final 1%, w/v) and protease inhibitor (Roche). After ultracentrifugation at 135,000 × g for 20 min at 25 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... diluted 10 times and 1 μl was used in 5-μl PCR reaction on a LightCycler 480 (Roche Diagnostics) with Luna Master Mix (New England Biolabs ...
-
bioRxiv - Developmental Biology 2020Quote: ... The resulting organoids were further digested with warm 0.25% trypsin for 1 min with constant pipetting followed by treatment with 5 mg/mL Dispase (Roche) for 5 min in at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... The E.coli cells were resuspended in lysis buffer (20mM HEPES, pH 7.5, 500mM NaCl, 5% glycerol supplemented with 1X PIC (Roche), 100ug/ml lysozyme and lysed by sonication and centrifuged ...
-
bioRxiv - Microbiology 2019Quote: ... were incubated 15 min in lysis buffer (20 mM HEPES buffer, pH 7.0; 100 mM NaCl; 5 mM MgCl2; 1% Triton X100; protease inhibitors (Roche)) and centrifuged for 15 min at 14 000 rpm in a refrigerated microfuge ...
-
bioRxiv - Microbiology 2019Quote: ... Transfected cells were washed two times in cold PBS and lysed in lysis buffer (20 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 0,2 % Triton X100, 10 % Glycerol, protease inhibitors (Roche)) ...
-
bioRxiv - Molecular Biology 2019Quote: ... up to 5 ng/sample of ChIP was prepared for sequencing using the Kapa Hyper-prep Kit (Kapa Biosystems) using NEXTflex adapters (Bio Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: ... frozen HEK293E cells were thawed and resuspended in 2x excess of lysis buffer (25 mM HEPES-KOH pH 7.6, 5 mM MgCl2, 50 mM KCl, cOmplete protease inhibitor cocktail (Roche), 1 mM PMSF ...
-
bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 150 mM NaCl, 5 mM EDTA, 2 mM ATP, 1 mM dithiothreitol and protease inhibitor cocktail tablets; Roche) using Bead Beater ...
-
bioRxiv - Biochemistry 2020Quote: ... bacterial pellets were resuspended using a high salt buffer (25 mM Tris pH7.5, 1 M NaCl, 10 mM imidazole, 5% glycerol) supplemented with protease inhibitor tablets (Roche), 0.5 mM PMSF ...
-
bioRxiv - Genetics 2020Quote: ... 721-bp tandem and 1600-bp tandem repeats were selected and PCR labelled with tetramethyl-rhodamin-5-dUTP (Roche) (Ishii et al ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were lysed in Buffer A (2 M NaCl, 20 mM HEPES pH 7.5, 20 mM Imidazole, 5 mM βME, protease inhibitor (Roche)) by sonication ...
-
bioRxiv - Microbiology 2019Quote: ... cells were lysed in ice-cold lysis buffer (10 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, supplemented with a protease cocktail inhibitor from Roche, Switzerland) for 30 minutes on ice ...
-
bioRxiv - Neuroscience 2019Quote: ... cortices from 6-week old mice were homogenized in cold sucrose buffer (20 mM HEPES pH 7.4, 320 mM sucrose, 5 mM EDTA) with protease inhibitor cocktail (Roche). Homogenates were centrifuged at 3,000 g for 20 min at 4°C speed to pellet nuclei ...
-
bioRxiv - Physiology 2021Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
-
bioRxiv - Cell Biology 2021Quote: ... except that 5 mg of synchronized HeLa cell lysate in 8 M Urea with protease inhibitor cocktail (Roche, 4693116001) was diluted by PBS buffer to a final urea concentration of 1 M before incubating with the conjugated beads ...
-
bioRxiv - Molecular Biology 2021Quote: ... Dechorionated embryos were then washed once with cold DEI buffer (10 mM HEPES pH 7.4, 5 mM DTT and 1x complete protease inhibitor from Roche) followed by homogenization in 1-2 embryo volumes of DEI buffer on ice ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES pH7.5, 2M NaCl, 5 mM MgCl2, 1 tablet of Complete Inhibitor cocktail EDTA Free (Roche) per 50◻ml ...
-
bioRxiv - Biochemistry 2021Quote: ... 20 mM imidazole and 5 mM β-mercaptoethanol (BME)) and supplemented with DNase I and protease inhibitor cocktail (Roche). Cells were disrupted by Emulsiflex C3 (Avestin ...
-
bioRxiv - Genomics 2019Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR analysis was performed by mixing 5 μL of 1 μmol·L-1 of both primers and 2.5 μL of 0.25 ng·μL-1 template DNA with 12.5 μL 2× KAPA HiFi HotStart ReadyMix (Roche, Basel, Switzerland). The thermal program was 98°C for 3 min ...
-
bioRxiv - Genomics 2021Quote: ... containing 25 Abs260 ribosome units (1 A260 = 12 μg/μL) [5] were digested with 60 U of MNase (Roche) and 60 U of SUPERase·In (Ambion ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 ml NP-40 in a total volume of 500 ml ddH2O) containing phosphatase and protease inhibitor cocktail (Roche) then centrifuged for 30 min at 12000 rpm at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.1% NP-40, and a protease inhibitor cocktail tablet [Roche]). After clarifying lysates by centrifugation at 2,000 rpm for 1 min at 4 °C twice ...
-
bioRxiv - Neuroscience 2022Quote: ... All cortices were homogenized in cold sucrose buffer (20 mM HEPES pH 7.4, 320 mM sucrose, 5 mM EDTA) supplemented with protease inhibitor cocktail (Roche) and a BCA was perform to assess the protein concentration of each sample ...
-
bioRxiv - Neuroscience 2022Quote: ... the neocortex from 8- 10 six-week-old mice was homogenized in cold sucrose buffer (20 mM HEPES pH 7.4, 320 mM sucrose, 5 mM EDTA) supplemented with protease inhibitor cocktail (Roche). Homogenates were centrifuged at low speed to pellet nuclei and cell debris (3,000 g for 20 min at 4_C ...
-
bioRxiv - Developmental Biology 2022Quote: ... DIG-labelled cDNA probe was incubated at 95 °C for 10 min and immediately placed in the ice for 5 min before adding to the hybridization buffer (5x SSC, 50 % formamide, 0.02 % SDS, 2 % blocking agent (Roche), DEPC-treated water) ...
-
bioRxiv - Genomics 2022Quote: ... 25 mM potassium chloride, 5 mM magnesium chloride, 10 mM Tris-HCl pH 8.0, 1 μM DTT, 1x Roche Protease Inhibitor Cocktail ...
-
bioRxiv - Biochemistry 2022Quote: ... The pellet was suspended in resuspension buffer (5% glycerol, 20 mM HEPES pH 7.4, 200 NaCl, cOmplete protease inhibitor mixture (Roche)) at a concentration of 4 mg/mL using a tissue grinder ...
-
bioRxiv - Biophysics 2022Quote: ... constructs were resuspended in Buffer C (20 mM at Tris pH 7.6, 300 mM NaCl, 5 mM DTT, and Roche Complete Mini protease inhibitor cocktail (Roche Diagnostics 11836153001)) ...
-
bioRxiv - Plant Biology 2022Quote: ... ground in a fine powder with liquid nitrogen and resuspended with 1 ml of Guanidine hydrochloride extraction buffer (6M GuaHCl, HEPES 1 M, EDTA: 5 Mm, adjusted to pH 7.5) supplemented with protease inhibitor cocktail (Roche). Homogenates were filtered through a 100 µm cotton mesh ...
-
bioRxiv - Plant Biology 2023Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
-
bioRxiv - Neuroscience 2022Quote: ... Each region was homogenized using Ribolyser for 5 min in 500uL lysis buffer (140mM NaCl, 20mM TrisHCl, pH 7.5, protease inhibitors [complete Mini, Roche] ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
-
bioRxiv - Neuroscience 2024Quote: ... .5 µL of a 10 µM primer mix and 7.5 µL of Fast Start SYBR Green Master Mix (Roche). Each reaction was performed in triplicate ...
-
bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... The template plasmid was diluted to 5×107 copies/µL with nuclease-free water plus yeast RNA (Roche, 10109223001), and this solution was further diluted to 2.5 ...
-
bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
-
bioRxiv - Molecular Biology 2024Quote: ... 150 mM NaCl, 0.5% NP40, 0.5% Triron-X100, 5% glycerol, 1x Roche Complete EDTA free inhibitor cocktail, 1x Roche PhosSTOP) for 1h on ice by agitation ...
-
bioRxiv - Microbiology 2024Quote: ... with their respective dilution in 5% skimmed milk in PBS-tween 0.1% were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...