Labshake search
Citations for Roche :
1001 - 1050 of 1062 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Pieces of tissue were incubated in 500 ml of NBT/BCIP solution (4.5 μl/ml NBT, 3.5 μl/ml BCIP in Alkaline Phosphatase buffer) (Roche 11383213001 and 11383221001) in the dark from some minutes to several hours ...
-
bioRxiv - Immunology 2024Quote: ... and 2 μL of the final cDNA solution is used in the subsequent quantitative polymerase chain reaction analysis (qPCR) with Sybergreen according to manufacturer’s instructions (Roche, Indianapolis, IN). Primer sequences are as follows.
-
bioRxiv - Developmental Biology 2024Quote: ... Samples subjected to colorimetric signal detection were incubated in anti-Digoxygenin-AP antibody solution (1:4000 dilution; Roche Diagnostics, Mannheim, Germany) overnight while rocking at 4°C ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Pellets were resuspended in 250 μL buffer L with sucrose (Buffer L, 250 mM sucrose, 0.5 mM DTT, 0.5 mM pefabloc, and 1x Roche protease inhibitor solution), spun at 1000 x g for 5 minutes at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: Tissue extracts were obtained by lysing E16.0 mouse cortical samples in radioimmunoprecipitation assay (RIPA) buffer supplemented with a protease inhibitor solution (cOmplete, EDTA-free Protease Inhibitor Cocktail; Roche, #11873580001) and 100mM PMSF at 4°C for 2 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... The tissues were then cut into small pieces using sharp dissection scissors and incubated in 10 mL dissociation solution (0.2 mg/mL Collagenase D (Roche, Cat#11088866001); 0.05 mg/mL DNase I (Roche ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 150 mM NaCl, 2% w/v PVPP, 10 mM DTT, 1× tablet of cOmplete™, EDTA-free Protease Inhibitor Cocktail [Roche], 0.1% Tween 20 [Sigma] per 50 ml), centrifuged at 4200 × g at 4°C for 20–30 min ...
-
bioRxiv - Neuroscience 2020Quote: ... the signal was visualized by incubation with nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP) solution (Roche Diagnostics, 11681451001) in 0.1 M Tris-HCl (pH9.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... mouse hind limb muscles (tibialis anterior, quadriceps and gastrocnemius) were minced and incubated in a 1 mg/ml collagenase/dispase solution (Roche, Basel, Switzerland) with agitation at 37°C for 1 hour ...
-
bioRxiv - Cancer Biology 2021Quote: We established single-cell suspensions of surgically resected tumors by taking a small piece of PDAC tumor tissue collected in a cell-dissociation media [5 ml of minimal essential media (MEM) containing 100μl of Liberase-TM (2.5 mg/ml stock solution Roche/Sigma Aldrich Cat# 5401046001), 50μl of Kolliphor®P 188 (15 mM stock solution Sigma Cat# K4894) ...
-
bioRxiv - Molecular Biology 2022Quote: Individual murine left atrial cardiac myocytes were isolated by perfusion with a Tyrode’s enzyme solution containing 20 μg/mL Liberase™ (Roche, Indianapolis, IN) or a collagenase+protease mix ...
-
bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and transferred in an enzyme solution (2 mg/mL Collagenase/Dispase (Roche, Bale, Switzerland), 0,147 µg/mL TLCK (Lonza ...
-
bioRxiv - Biochemistry 2021Quote: The pupae infected by the baculovirus were homogenized with a buffer solution (20 mM Tris-HCl, 150 mM NaCl, 5 mM DTT, 1mg/ml phenylthiourea, one inhibitor cocktail tablet (EASYpack, Roche, Basel, Switterland) per 50 ml solution ...
-
bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37 °C in the dark for 1 h with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) solution from the In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). The embryos were washed three times with PBS-PVP ...
-
bioRxiv - Microbiology 2023Quote: ... The membranes were developed in a NBT/BCIP solution (nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indoxyl phosphate; Roche; Basel, CH) for up to 20 min at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated with a solution containing horseradish peroxidase (HRP)- conjugated anti-Dig (at a dilution of 1:500; #11207733910, Roche Applied Science) and goat anti-mCherry (at a dilution of 1:250 ...
-
bioRxiv - Genomics 2024Quote: ... Hybridization was carried out with SeqCap RNA Choice Probes using the KAPA HyperCapture Reagent and Bead kit (v2, Roche Sequencing Solutions, Inc.) according to SeqCap RNA Enrichment System User’s Guide (v.1.0 ...
-
bioRxiv - Neuroscience 2023Quote: ... in Tris-EDTA buffer pH 7.8 at 95°C for 64 min in standard cell condition solution one (CC1) using a Ventana Discovery ULTRA (Roche Indianapolis IN). Blocking was then performed in an inhibitor solution for 12 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
-
bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
-
bioRxiv - Microbiology 2022Quote: ... Adapter ligation was performed for 1 hour at 20°C using the Kapa Unique-Dual Indexed Adapters diluted to 1.5 μM concentration (Roche Sequencing Solutions Inc.). After ligation ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membranes were developed in a NBT/BCIP solution (nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indoxyl phosphate; Roche; Basel, CH) for up to 20 min at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were incubated overnight at 60°C in 100 μl of hybe-solution buffer containing 20 ng digoxigenin (Dig)-labeled probes (see below) for colorimetric detection (Roche, cat. #11277073910) or 20 μg of fluorescein-labeled probes (see below ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the immunohistochemical reaction was developed using 3,30-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (Chromomap DAB, Ventana, Roche or DAB solution, Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
-
bioRxiv - Genomics 2024Quote: ... Both DNA samples were also converted into Illumina sequencing libraries with the KAPA HyperPrep library construction kit (Roche Sequencing Solutions, Indianapolis, IN) and paired-end sequenced for 150 cycles on a NovaSeq6000 (Illumina ...
-
bioRxiv - Cell Biology 2024Quote: ∼ 200 embryos of 1dpf were placed in 1mL modified Ringer’s solution (116 mM NaCl, 3 mM KCl, 4 mM CaCl2, 5 mM HEPES; pH 7.5) containing Protease Inhibitor (Roche, Cat. No. 04693132001) and Phosstop (Roche ...
-
bioRxiv - Molecular Biology 2024Quote: ... Alkaline phosphatase staining was conducted on primary OP6 cell cultures on Day-5 post-transfection using NBT/BCIP stock solution (Roche/Sigma 11681451001) or BCIP/NBT-Blue Liquid Substrate Systems for Membranes (Sigma B3804 ...
-
bioRxiv - Physiology 2024Quote: ... Solution was switched to a digestion solution: the modified Tyrode above containing 100 µM CaCl2 and 0.04 mg/ml Liberase™ (Roche, Penzberg, Germany) and no EGTA ...
-
bioRxiv - Physiology 2024Quote: ... Solution was switched to a digestion solution: the modified Tyrode above containing 100 µM CaCl2 and 0.02 mg/ml Liberase™ TH (Roche, Penzberg, Germany) and no EGTA ...
-
bioRxiv - Neuroscience 2021Quote: Sections from adra1A KO mice were rinsed with PBS (3 × 5 min) and incubated in a β-gal staining solution (Roche, Ref # 11828673001) overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... SeqCap library preparation was performed using custom Nimblegen SeqCap probes (described above in §2.1) according to the NimbleGen SeqCap EZ HyperCap Workflow User’s Guide Ver 2 (Roche Sequencing Solutions, Inc., CA USA). Following capture ...
-
bioRxiv - Cell Biology 2020Quote: ... The solution was refreshed one time before the addition of Liberase TM research grade (final concentration 0.038 mg/mL (Roche Diagnostics, GmbH, Mannheim, Germany)) and Elastase from porcine pancreas (final concentration 0.01 mg/mL (Bio-Connect B.V. ...
-
bioRxiv - Cancer Biology 2020Quote: ... 80 µl of dephosphorylation solution (8 μl 10x dephosphorylation buffer, 2 μl RNasin Plus, 67 μl ddH2O, and 3 μl alkaline phosphatase (Roche, catalog no. 10713023001)) was added to the beads ...
-
bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
-
bioRxiv - Synthetic Biology 2020Quote: Voltage signal events were converted to raw reads using a commercial probabilistic base-calling algorithm (version 2.9.2, Roche Sequencing Solutions, Santa Clara, CA). Raw reads ...
-
bioRxiv - Cell Biology 2020Quote: ... and then digested for 1 h at 37°C in a vigorously shaking solution consisting of 16 mg of Collagenase D (Roche, Pleasanton, CA, USA), 3.0 U of Dispase II (Roche) ...
-
bioRxiv - Immunology 2021Quote: ... Lungs were mechanically dissociated by using a scalpel and transferred to collagenase solution consisting of DMEM supplemented with collagenase D (Roche, 1 mg/ml) and DNase1 (ThermoFisher ...
-
bioRxiv - Immunology 2024Quote: ... Brains were homogenized using a glass Potter and digested for 45 min at room temperature (RT) in Hanks’ balanced salt solution (HBSS) medium with collagenase D (1 mg/ml, Roche Diagnostics cat # 11088882001) and deoxyribonuclease (DNase ...
-
bioRxiv - Plant Biology 2024Quote: ... Samples were mounted in Citifluor AF1(Agar Scientific, Cat No: AGR1320)/PBS solution (3:1) with (optional a DAPI (Roche, Cat No: 10236276001) to a final concentration of 10µg.ml−1 ...
-
bioRxiv - Bioengineering 2022Quote: ... and the cell pellet was re-suspended in 2 ml of triturating solution (containing 10 mg/ml bovine serum albumin [A7906, Sigma], 0.5 mg/ml trypsin inhibitor [10109886001, Roche], 0.02 mg/ml deoxyribonuclease).
-
bioRxiv - Immunology 2023Quote: ... The gut was then added to a 1.5 mL Eppendorf tubed filled with 1 mL pre-warmed 37°C collagenase/dispase solution (Roche 10 269 638 001) and incubated for 90 seconds before adding to a 40-micron filter as above ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
-
bioRxiv - Cancer Biology 2024Quote: ... A total of 19 hindguts were minced with a blade and incubated for 30 min at 37°C in a solution of Collagenase D (Roche, 1 mg/mL). After filtering in a 40 μM strainer and washing with BGJb ...
-
bioRxiv - Bioengineering 2024Quote: ... the D-PBS was removed from all the middle channel inlets and 50 µL of 28.5 µg/ml fibronectin solution (10 838 039 001; Roche Diagnostics Corp., Indianapolis, IN) in non-supplemented MCDB131 medium was added to all the top channel outlets ...
-
bioRxiv - Genetics 2022Quote: ... Embryos were then washed 3x 5 minutes in TBS-T (1% Tween-20 in Tris-buffered saline) and blocked for 1 hour in block solution (10% heat-inactivated sheep serum and 0.1% Roche blocking reagent in TBS-T). Roche blocking reagent was dissolved in maleic acid buffer according to manufacturer’s recommendations ...
-
bioRxiv - Physiology 2022Quote: ... This was followed by a perfusion at 4 mL/min for 40 min with the same solution containing 1 mg/mL of collagenase A (Roche Diagnostics GmbH, Mannheim, Germany) plus 300 µM ethylene glycol tetraacetic acid (EGTA ...
-
bioRxiv - Zoology 2020Quote: ... Next the 1% BSA in PBST is replaced with the same solution to which both sheep anti-digoxygenin Fab-fragments conjugated to alkaline phosphatase (Roche Diagnostics GmBH, Mannheim, Germany) in a 1:1,000 dilution and SIFamide antiserum in a 1:2,000 dilution have been added ...
-
bioRxiv - Cancer Biology 2021Quote: Tissue sections were dissected using the reference mask image from serial section 6 to collect regions of interest using medium or large AVENIO Millisect milling tips (Roche Sequencing Solutions, Pleasanton, CA), collected with Molecular Grade Mineral Oil (Sigma-Aldrich ...
-
bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science, Mannheim, Germany) at 37°C for 1 hour ...