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Citations for Roche :
901 - 950 of 1062 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression signals were visualized using nitroblue tetrazolium/5-bromo-4-chloro-3-indolylphosphate solutions using a standard method (Roche). Whole-mount and sectioned specimens of Ciona were observed using an SZX12 stereo microscope (Olympus ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue and primary hippocampal neurons cells were homogenized in an ice-cold lysis buffer supplemented with phenylmethanesulfonyl fluoride solution and a protease inhibitor cocktail with EDTA (Roche). Whole-cell lysates were further homogenized by passage through a 26-gauge syringe 5–6 times ...
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bioRxiv - Microbiology 2020Quote: ... The infected cells were harvested in RIPA buffer solution supplemented with cOmplete™ and EDTA-free Protease Inhibitor Cocktail (Roche). Protein quantification was performed using the Bradford Reagent (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low and high stringency washes ...
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bioRxiv - Bioengineering 2021Quote: Cell-seeded scaffolds were collected at weeks 0 and 4 and digested in proteinase K solution (0.5 mg/ml in phosphate buffer, pH 6.5, Roche, Basel, Switzerland) for 48 hours at 56 °C ...
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bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The blocking solution was removed and replaced with antibody solution containing 1% heat inactivated goat serum and a 1:5000 dilution of anti-DIG-AP antibody (Roche). Slides were then incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Microbiology 2021Quote: ... 5 ul of this solution was used for amplification of the SLST region linked to illumina adaptors using KAPA HiFi HotStart Readymix (Roche) (Initial denaturation for 5 min at 95 °C followed by 35 cycles of 98 °C for 20 s ...
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bioRxiv - Microbiology 2020Quote: Further protein extraction steps for each of 10 samples were carried out in solutions supplemented with cOmplete protease inhibitors (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... and nuclear extracts prepared by suspending nuclei in NER solution in the presence of protease inhibitors (Complete EDTA-free Protease Inhibitor Cocktail, Roche) and digestion or not with Benzonase (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... for 1h at RT and incubated with a primary antibody diluted in blocking solution (sheep anti-digoxigenin, 1/200, Roche) overnight at +4°C in a dark humidified chamber ...
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bioRxiv - Biochemistry 2022Quote: ... samples were incubated with 50 μl mix of 5 μl enzyme and 45 μl labeling solution (TUNEL In situ Cell Death Detection Kit, TMR Red’ from Roche) for 1.5 h at 37°C in a dark humid chamber ...
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bioRxiv - Neuroscience 2022Quote: ... Combined brain and spinal cord tissues were homogenized with an 18-gauge needle in a protease inhibitor solution created using protease inhibitor cocktail tablets (Roche) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and prepared for flow cytometry immunostaining as follows: Matrigel-embedded organoids were digested using a HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml of collagenase type IV (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... 150 mg of frozen powder were added to a 1.5 mL microcentrifuge tube and resuspended in 650 μL lysis buffer (20 mM Tris pH 8.5, 150 mM KCI, 0.1% Tween 20 and 1x cOmplete Mini EDTA-free protease inhibitor solution; Roche Life Science). The suspension was spun at 14,000 g for 10 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche] ...
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bioRxiv - Developmental Biology 2022Quote: ... Signal was revealed with 4 μL of NitroBlue Tetrazolium/5-Bromo-4-Chloro-3-Indolyl Phosphate (NBT/BCIP) Stock Solution (Roche)/ml of AP reaction buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were then incubated overnight at 4 °C with primary antibody of interest diluted in blocking solution (Western Blotting Reagent, Roche). The appropriate species of HRP-conjugated secondary antibody (from Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 30 min at 37 °C in a solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were treated for 1 hr at 55⁰C with proteinase K solution (0.4 mg/mL proteinase K (Roche; 3115828001), 50 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC lysis buffer (1.5 ml buffer per gram cell pellet) and the complete stock solution (1 tablet Complete EDTA-free (protease inhibitor cocktail, Roche) and 50 μl benzonase nuclease cell resuspension (PSF ...
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bioRxiv - Genomics 2022Quote: Human embryonic hindlimb muscles were collected in sterile PBS and mechanically dissociated using fine scissors in 10 mL (per gram of tissue) of enzyme solution containing 2.5 U/mL Dispase II (Roche, #4942078001) and 1 mg/mL Collagenase B (Roche ...
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bioRxiv - Genomics 2022Quote: ... Tissue was then mechanically dissociated with fine scissors in 10 mL (per gram of tissue) enzyme solution mixed with 2.5 U/mL Dispase II (Roche, #4942078001) and 1 mg/mL Collagenase B (Roche ...
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bioRxiv - Microbiology 2021Quote: ... were added (0.5 g to each 100 mg pellet) along with protease inhibitor solution (complete mini EDTA-free protease inhibitor cocktail, Roche), dH2O and 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Pathology 2021Quote: ... The sections were incubated for 1 to 24 hr in the dark at room temperature with development solution (NBT/BCIP substrate, Roche)
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bioRxiv - Zoology 2020Quote: ... the digoxygenin probe is visualized by replacing the APB is replaced with the same containing 20 µl/ml of a commercial NBT/BCIP stock solution (Roche Diagnostics GmBH ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% Tween 20 in water) and incubated in NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate) solution (Roche).
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bioRxiv - Immunology 2021Quote: ... the dura mater was carefully removed from the brain and then chopped for enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 15 mg/mL collagenase D (Roche) and 1 mg/mL DNase (Roche ...
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bioRxiv - Microbiology 2021Quote: ... GTP binding Irgb6 crystals diffracting to 1.5 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GTP (Roche) and a reservoir solution consisting of 0.1 M Sodium Citrate buffer pH 5.4 (Wako) ...
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bioRxiv - Neuroscience 2021Quote: ... MGEs pooled from a single litter were incubated with 200 µL papain solution supplemented with 10 U/mL recombinant DNase I (Roche) for 10 minutes at 37 °C on a rocking platform ...
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bioRxiv - Developmental Biology 2021Quote: ... The testicular tissue was finely minced and incubated in a dissociating solution containing 40 mg/mL Collagenase H (Roche, Switzerland), 33.3 mg/mL Dispase II (Roche) ...
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bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated overnight at 4°C with an anti-DIG-AP antibody (Roche, diluted 1:2000 in blocking solution). The next day ...
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bioRxiv - Molecular Biology 2021Quote: ... An equal volume of lysis buffer and 110 U/ml of DNase (DNase I recombinant, RNase-free solution, Cat no. 4716728001, Roche) was added to the ground cell lysate followed by incubation for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with blocking solution for 1 hour and finally incubated overnight at 4°C with anti-Fluorescein-POD antibody (Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Molecular Biology 2022Quote: ... The inner bottom surface of the flow cell is coated with an hydrophobic solution (nitrocellulose or Sigmacote) to allow attachement of anti-Digoxigenin (11093274910, Roche) Lionnet et al ...
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bioRxiv - Molecular Biology 2022Quote: Mouse pancreata from adult mice (9-14 week old) were perfused with a solution containing 1 mg/mL collagenase-P (Roche) in Hank’s buffered salt solution (HBSS ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.05% Tween 20) before exchange of the pre-incubation solution for hybridization buffer containing 3 ng/µl of digoxigenin-labeled (Roche) riboprobe ...
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bioRxiv - Developmental Biology 2022Quote: ... Matrigel drops (at least 4 samples per time point) were first digested with HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml collagenase type IV (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The solution was incubated for 20 min at 37°C and 15 μl of proteinase K (20 g L-1l; Roche) was added ...
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bioRxiv - Microbiology 2022Quote: ... GTP-binding Irgb6-T95D crystals diffracting to 1.68 Å resolution were obtained from sitting drops with a 0.5 μl of protein solution containing 2 mM GTP (Roche) and a 0.5 μl of reservoir solution consisting of 0.2 M Sodium sulfate (Molecular Dimensions ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue fragments were dissected with sterile scissors in a FBS-free DMEM solution containing 1x Liberase TM Research Grade (Roche) and Antibiotic-Antimycotic (Gibco) ...
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bioRxiv - Plant Biology 2022Quote: ... The fixed pre-parasitic J2s were mechanically cut into fragments and enzymatically permeabilized with proteinase K (0.5 mg/mL) solution (Roche, Germany). Hybridization with sense (negative control ...
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bioRxiv - Plant Biology 2022Quote: Total protein was extracted from 6 dpi infiltrated leaf patches of WT plants using protein extraction buffer (1M Tris-HCl, PH 8.8, 10% Glycerol, 10% SDS, and H2O) together with cOmplete™ Protease inhibitor solution (Roche). SDS-PAGE was performed for Coomassie staining and Western blotting (Wb) ...
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bioRxiv - Neuroscience 2022Quote: One adult rat brain (2 g) was homogenized in nine volumes of 320 mM sucrose solution supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and Phosphatase Inhibitor Cocktail 1 and 2 (Sigma ...
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bioRxiv - Physiology 2023Quote: ... and then incubated for 45 min at 37°C either in Hanks’ Balanced Salt solution (HBSS) or plain DMEM media supplemented with Liberase HD (200 μg/ml, Roche) and DNase I (40 U/ml ...
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bioRxiv - Physiology 2024Quote: ... pancreas was distended by cannulizing the bile duct to infuse 2.5ml of collagenase P (0.66mg/ml in Hank’s Balanced Salt Solution) (Roche, catalog number C7657), surgically removed and incubated at 37°C for 15 minutes under constant agitation ...
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bioRxiv - Pathology 2024Quote: Protein lysate samples were prepared from snap frozen aortas and cells in RIPA solution (Cat# P0013B; Beyotime) supplemented with protease inhibitor (Cat# 05892791001; Roche). Denatured protein lysates were resolved by 8% −10% (wt/vol ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate; Roche) until the staining appeared (overnight or up to 48 h) ...