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Citations for Roche :
851 - 900 of 1062 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: HEK293T cells transiently transfected with WT EphA2-eYFP were selected with 1.6 mg/ml G-418 solution (Roche; 4727878001) for 12 days to generate a stable cell line ...
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bioRxiv - Pathology 2021Quote: ... adjust pH to 9.5 adding HCl) for 2 minutes and then tissue was covered with NBT/BCIP stock solution (Roche) diluted in 0.1M TRIS buffer (pH 9.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos are rinsed in TBS/0.1% Tween-20 (TBST) then blocked for 2 h in 2% blocking solution (Roche) and incubated O/N in the same solution containing 1:2,500 anti-digoxigenin antibody (Roche) ...
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bioRxiv - Immunology 2021Quote: ... brain and dura mater were chopped and subject to enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 2.5 mg/mL collagenase D (Roche) and 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... brains were dissected in a cold Ringer’s solution and dissociated by incubation with collagenase/dispase mix at 29°C (Roche, 15 minutes for larval and pupal brains and 30 minutes for adult brains) ...
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bioRxiv - Cell Biology 2021Quote: ... for 90 minutes at 37°C and digested overnight at 37°C in 1.5 mg/ml collagenase B solution (Roche) under continuous agitation ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2hr at room temperature and then incubated in blocking solution containing anti-Digoxigenin-AP antibody (1:2000; Roche) overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
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bioRxiv - Immunology 2020Quote: ... tissue was cut into pieces and digested for 15 minutes in a shaking water bath held at 37°C in a 0.5mg/ml solution of Dispase II (Roche) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... a 1% agarose gel block embedded with enterococci was treated with lysozyme solution (10 mg/mL; Roche, Basel, Switzerland) at 37 °C for 6 h ...
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were washed with 2 X SSC and 0.2 X SSC for 20 min followed by incubation in a blocking solution containing 2% blocking reagent (Roche) and 20% sheep serum for 1 hr ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were incubated in a blocking solution containing maleic acid buffer (pH 7.5) and 1 % blocking reagent (Roche). Subsequently ...
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bioRxiv - Physiology 2023Quote: ... enzymatic digestion was carried out for 20-25 min at 36 °C in the low-Ca2+ solution containing Liberase TH Research Grade (0.15 mg/mL; Roche) and elastase (0.5 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was transferred to a blocking buffer (1 M maleic acid solution pH7.4, 1% nucleotide blocking reagent (Roche)) for 30 minutes and then incubated in antibody-solution (anti-dioxigenin-AP fragments in blocking buffer) ...
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bioRxiv - Neuroscience 2023Quote: ... then transferred for 48 hrs in a solution of mouse anti-synaptophysin (1:20, Roche Applied Science, Indianapolis, IN). Sections were then washed in PBS-t 3x10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of next generation sequencing libraries was done using the KAPA HyperPrep ChIP Library kit (Roche Sequencing solutions) at the molecular biology and functional genomics platform of the Institut de Recherche Clinique de Montréal (IRCM) ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with 1 nM DIG (Digoxigenin)-labeled Telomere probes in Hybridization solution (DIG Easy Hyb, Roche) at 42°C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA amplification was performed in 50 µL PCR solution [1X Hifi Hot Start Ready mix (Kapa Biosystems, cat# KK2601), 1 µM SMRTpcr primer (Supplementary Table S6)] ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Plant Biology 2023Quote: ... The reaction solution was then combined with 50 μl IP buffer (PBS supplemented with 0.005% NP40 and proteinase inhibitors (Roche)) and mixed with 20 μl anti-FLAG magnetic beads (Merck Millipore M8823) ...
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bioRxiv - Cell Biology 2023Quote: The Haemolymph-Like Salines (HL3 solutions) were prepared as described previously (Roche et al., 2002; Ataman et al., 2008). The list of all the solutions used is given below (Table 2).
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bioRxiv - Systems Biology 2023Quote: Glucose concentrations from the chemostat samples were determined enzymatically with a solution of hexokinase/glucose-6-phosphate dehydrogenase (Roche) in Pipes buffer at pH 7 ...
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bioRxiv - Cell Biology 2023Quote: ... Hybridization of the first PNA probe (TelC-Cy3) was then carried out in hybridization solution (70% deionized formamide, 0.25% blocking reagent (Roche), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Genomics 2023Quote: Fresh pinch biopsies were washed twice in DPBS before being coarsely chopped with a scalpel and transferred into 0.5 mL DPBS solution containing 0.1 WU ml−1 Liberase TH (Roche, 5401151001) and 0.5 U ml−1 RQ1 DNase I (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... The slides were washed and detection was performed at pH 9.5 by incubating in nitro blue tetrazolium and 5-bromo-4-cholro-2-indoyl phosphate solution (Roche) as per manufacturer instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The left lobes of the lung were then inflated with digestion solution containing 1.5 mg/mL of Collagenase A (Roche) and 0.4 mg/mL DNaseI (Roche ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... and reconstituted at 0.5mg/ml in water and then further diluted to a 0.1 mg/ml stock solution in PBS (gibco) 0.1% BSA (Roche, #10735086001). Agarose was purchased from Invitrogen (#16500-500).
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bioRxiv - Evolutionary Biology 2023Quote: ... 500 ng of DNA from each library was pooled and hybridized in solution to the SeqCap EZ probes following the manufacturer’s protocols (Roche Sequencing Solutions ...
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bioRxiv - Genetics 2023Quote: ... the mixture was incubated at 42 °C overnight and then added to 10 ml DIG-Easy hybridization solution (Roche).
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bioRxiv - Immunology 2023Quote: Skin samples collected from the ventral neck area of 4-5 mice were minced in Hank’s balanced solution (HBSS) and incubated at 4°C overnight with 1.5mg/ml dispase II (Roche; 25766800). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE slides were steamed for 48 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Both forebrains and connective tissues were dissociated in the solution containing 2mg/ml Collagenase/Dispase (Roche, Basel, Switzerland; 10269638001) in Hanks’ Balanced Salt solution ...
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bioRxiv - Immunology 2024Quote: Lung tissue was prepared by coarsely chopping the tissue followed by incubation in a 0.5 mg/mL solution of collagenase D (Roche) and 500 Units of DNase (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in blocking solution containing 5% horse serum and 1% Western Blocking Reagent (Roche Diagnostics, Mannheim, Germany) in TNTx for at least 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... protease inhibitor (1 tablet per 10 ml solution; cOmplete ULTRA Tablets-Mini, F. Hoffmann-La Roche AG, Basel, Switzerland) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells are then washed twice in cold 1% BSA solution containing 0.5X EDTA-free Protease inhibitor cocktail (PIC, Roche), and once in cold PBS ...
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bioRxiv - Neuroscience 2024Quote: ... followed by the addition of 350ul of calcium- and magnesium-free Dulbecco’s balanced salt solution with protease inhibitor cocktail (Roche Applied Sciences ...
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bioRxiv - Developmental Biology 2024Quote: ... animals were incubated in MABTw blocking solution (5% heat-inactivated horse serum, 5% Roche Western Blocking Buffer in MABTw) for 2 hours at room temperature and then incubated in anti-DIG-AP (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was suspended using Buffer A (25 mM Mops-KOH, pH 7.5, 200 mM NaCl, 10% Glycerol, and cocktail protease inhibitor solution (Roche) with 50-fold dilution) ...
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bioRxiv - Cancer Biology 2024Quote: ... the single cells of each litter were stained for the epithelial marker Epcam in 500 μL staining solution (PBS (gibco) + 0.2% BSA (Roche) + 1 μL Epcam-PE-Cy5 (eBioscience)) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS and resuspended in preheated (80°C for 5 min) hybridization buffer (20 mM Tris pH 7.4, 60% formamide, 0.5% blocking solution (Roche #11096176001) and 1:300 dilution of PNA TelC-AF488 probe (PNA Bio #F1004)) ...
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bioRxiv - Cell Biology 2024Quote: ... glass slides and incubated with a preheated (80°C for 5 min) hybridization buffer (20 mM Tris pH 7.4, 60% formamide, 0.5% blocking solution (Roche #11096176001) and 1:200 dilution of PNA TelC-AF488 probe (PNA Bio #F1004) ...
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Liver Sinusoidal Endothelial Cells and Laminin dictate cholangiocytes’ fate in chronic liver diseasebioRxiv - Cell Biology 2024Quote: ... liver were dissociated using 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Pronase protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.2 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were allowed to air dry fully before applying Hybridizing Solution (70% formamide, 1 mg/ml blocking reagent (Roche), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Neuroscience 2021Quote: ... and overlaid on top of 29% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 29% Iodixanol, 1x protease inhibitor (Roche, 11873580001), 0.4 U/ul RNAse inhibitor (Takara ...
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bioRxiv - Developmental Biology 2021Quote: ... after fixation in 4% formaldehyde and bleaching (Pearson et al., 2009) using blocking solution containing 10% horse serum and western blot blocking reagent (Roche) (King and Newmark ...