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Citations for Roche :
951 - 1000 of 3681 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems) in 20 μl reactions ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification of targeted sequence were performed with KAPA HiFi HotStart ReadyMix PCR kit (Roche, Germany) and the following program ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative PCR was performed using KAPA SYBR Green fast Master PCR Kit (Roche SFUKB-KK (04707516001). Generation of specific PCR products was confirmed by melting-curve analysis ...
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bioRxiv - Biochemistry 2021Quote: ... All PCR were performed using the Expand High Fidelity PCR System (Roche Diagnostics GmbH, Mannheim, Germany) and primers were designed using the online tool at www.leishgedit.net ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR-green based quantitative real-time PCR (qRT–PCR) was performed on a LightCycler 480 (Roche). Ct values were normalized to the housekeeping gene TBP for human samples and to Actb or Ccny for the mouse samples ...
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bioRxiv - Genomics 2021Quote: ... The resulting libraries were amplified with 4 PCR cycles using KAPA HiFi Uracil+ PCR enzyme (Roche). Libraries were quality controlled on a TapeStation 2200 HSD1000.
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bioRxiv - Cell Biology 2019Quote: ... qRT-PCR was performed using the light Cycler 480 II real-time PCR machine from Roche and SYBR green detection.
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bioRxiv - Genomics 2021Quote: ... and whole transcriptome amplification (WTA) by PCR was performed using KAPA Hifi PCR Mastermix (Kapa Biosystems). WTA product was purified using Agencourt Ampure beads (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The PCR was conducted using a Kapa 2G Robust PCR kit (Kapa Biosystems, Woburn, MA, USA) in 25 µ L reactions containing ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using a LightCycler® 96 System (Roche Diagnostics) with the Thunderbird SYBR Green PCR Mix (Toyobo) ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was performed with a LightCycler 480 Real-Time PCR system (Roche Diagnostics, Mannheim, Germany) using the specific primers and LightCycler-DNA Master SYBR Green I (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with FastStart Essential DNA Green Master (06402712001, Roche) with 25 ng of cDNA per reaction (also ...
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bioRxiv - Genomics 2023Quote: ... PCR amplifications were performed with Expand Long Template Enzyme mix (Expand Long Template PCR system, Roche). Oligos are available in Table S4.
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bioRxiv - Biochemistry 2023Quote: ... All PCRs were performed using the Expand High Fidelity PCR System (Roche Diagnostics GmbH, Mannheim, Germany) and primers were designed using the online tool at www.leishgedit.net ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using the Expand High Fidelity PCR System dNTPack (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Neuroscience 2023Quote: Real-time PCR analysis was performed with use of LightCycler 96 real-time PCR system (Roche). The PCR parameters were following ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR analysis was performed using a LightCycler® 96 Real-Time PCR System (Roche). Primers were designed for each gene with Primer3-0.4.0 software (http://bioinfo.ut.ee/primer3-0.4.0/) ...
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bioRxiv - Plant Biology 2024Quote: ... q-PCR reactions were performed using FastStart Universal SYBR® Green Master (ROX) PCR mix (Roche) in a StepOne machine (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR analysis was performed using a LightCycler® 96 Real-Time PCR System (Roche). RT-qPCR reaction was prepared in triplicates by mixing 2.5 μl of a 25-fold diluted cDNA ...
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bioRxiv - Plant Biology 2024Quote: ... Polymerase chain reactions (PCRs) were done employing the KAPA HiFi HotStart ReadyMix PCR Kit (#KK2601; Roche Sequencing ...
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bioRxiv - Genetics 2024Quote: ... and PCR amplified using the KAPA HiFi Uracil PCR Kit (Catalogue Number: ROC-07959052001, Kapa Biosystems). The final libraries were assessed with the Agilent 2200 Tapestation System using D1000 Kit (Catalogue Number:5067-5582) ...
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bioRxiv - Synthetic Biology 2021Quote: ... KAPA2G Robust PCR kits (Roche KK5005) were used to perform colony PCR and multiplex PCR for a screening of Yeast Artificial Chromosomes (YACs) ...
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bioRxiv - Developmental Biology 2019Quote: ... dissolved in PCR grade water (Roche) at 3µg/µl and stored in aliquots at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... as determined by quantitative PCR (Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Test version 2.0 ...
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bioRxiv - Microbiology 2019Quote: ... [22] using RT-PCR reagents from Roche PCR Kit (Roche Diagnostics, Meylan, France) and primers and probes as previously described [21] in a CFX 96™ real-time PCR thermocycler and detection system (BIO-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... qRT-PCR was performed by Roche Light Cycle 480 fluorescent quantitative PCR instrument ...
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bioRxiv - Physiology 2020Quote: ... Real-time PCR (LightCycler 480; Roche) was performed with diluted cDNAs in a 20-μl reaction volume in triplicate.
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bioRxiv - Genetics 2019Quote: ... 1× FastStart PCR Reaction buffer (Roche) (50mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and a LightCycler PCR instrument (Roche). The real-time PCR primers are listed in Supplemental Table 4 ...
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bioRxiv - Immunology 2022Quote: ... as assessed by qRT-PCR (Roche LightCycler480 ...
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bioRxiv - Synthetic Biology 2023Quote: ... KAPA HiFi HotStart PCR Kit (Roche), Micellula DNA Emulsion & Purification Kit (EURx) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Real-Time PCR (KAPA Biosystems). Sequencing libraries were clustered onto a lane of a flow cell ...
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bioRxiv - Cell Biology 2019Quote: ... 3) 2hr RT incubation in blocking solution (5% Sheep Serum, 1% Roche Blocking Buffer in PBST); 4 ...
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bioRxiv - Immunology 2022Quote: Full-length HIV clones were quantified by reverse transcriptase (RT) enzyme-linked immunosorbent assay (ELISA) (Roche) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR was performed by using the Bestar SybrGreen qPCR Mastermix (DBI) and LightCycler 480II (Roche). For calculation the relative expression levels of tested genes ...
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bioRxiv - Molecular Biology 2019Quote: ... Meg3 and Dlk1 expression were quantified by RT-qPCR using SYBR Green I master mix (Roche) on a Lightcycler 480 instrument ...
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bioRxiv - Microbiology 2019Quote: ... The SIV3vpx particles were quantified after thawing using a reverse transcriptase (RT) assay colorimetric kit (Roche) following the manufacturer’s instructions to provide a RT ng/mL titre.
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bioRxiv - Evolutionary Biology 2020Quote: ... RT-qPCR was performed using 2X SYBR Green I master mix (Kapa Biosystems, Cat. No. KK3605) and the reaction was carried out in Roche LightCycler 480 (USA ...
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bioRxiv - Plant Biology 2021Quote: ... We performed targeted RT-qPCR on specific plant transcripts in a LightCycler (LightCycler 480; Roche Diagnostics) as previously described (Girin et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Additional RT-qPCR was performed using SYBR Green without the ROX reference dye (Roche Applied Science) and the QuantStudio 6 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000, Roche 11093274910) in Blocking Buffer ...
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bioRxiv - Pathology 2021Quote: ... was reverse-transcribed utilizing the RT Easy II First Strand cDNA Synthesis Kit (04379012001, Roche, Switzerland). We amplified cDNA (18 ng ...
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bioRxiv - Neuroscience 2019Quote: ... Probes were detected using a 1h incubation at RT with anti-fluorescein-POD (1:2500, Roche) and anti-DIG-POD antibodies (1:2500 ...
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bioRxiv - Physiology 2021Quote: ... The expression was determined by RT-qPCR using a LightCycler 480 or Nano (Roche, Mannheim, Germany) with SYBR Green I Master Mix (Roche ...
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bioRxiv - Immunology 2020Quote: ... quantitative polymerase chain reaction (RT-qPCR) was performed with SYBR FAST qPCR Master Mix (Kapa Biosystems) with primers shown in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... and then blocked in TNT/block [0.5% blocking reagent (PerkinElmer FP1012)/TNT] for 1 hour at RT before ON incubation with anti-DIG (1:100, Roche 11207733910) or anti-Fluo (1:250 ...
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bioRxiv - Molecular Biology 2022Quote: ... MAD2L1 was detected by RT-qPCR analysis using the SYBR Green detection system (Roche Applied Science) and normalized to the level of the GAPDH mRNA expression and using the 2-ΔΔCT cycle.
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bioRxiv - Neuroscience 2022Quote: ... then incubated for four hours at RT in AP-conjugated anti-DIG Fab fragments (Roche, 11093274910) diluted 1:2000 in 2% DIG blocking reagent ...
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bioRxiv - Pathology 2023Quote: ... JCV levels per tissue were quantified by RT-qPCR using a LightCycler® 480 instrument (Roche), the copies of JCV per reaction were multiplied by ten to obtain the copies of JCV per tissue ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The remaining RT products were collected and amplified by KAPA HiFi Hotstart Ready Mix (Roche, KK2602) with 0.8 µM cDNA-PCR primer ...