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Citations for Roche :
901 - 950 of 3681 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was carried out using a LightCycler 480 SYBR Green I Master Mix (Roche). Rpl32 (LOC5577996 ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were blocked in 1% blocking solution (10 min at RT; Roche Applied Sciences) and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000 ...
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bioRxiv - Cell Biology 2019Quote: ... RT qPCR was performed using the LightCycler® 480 Probes Master mix (Roche Applied Science) on a LightCycler® 480 (Roche Applied Science) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was then amplified in RT-qPCRs using LightCycler 480 SYBR Green I Master (Roche) and transcript-specific primers on a Roche LightCycler 480 thermocycler following standard protocol (45 cycles ...
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bioRxiv - Plant Biology 2019Quote: ... then analyzed by RT-qPCR using LightCycle FastStart DNA MasterPLUS SYBR Green I kit (Roche) with a LightCycler 480 (Roche ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR assays were performed with SYBR FAST qPCR Master Mix (Kapa Biosystems, Cat# KK4602), using primers indicated in Supplementary Table 2 ...
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bioRxiv - Bioengineering 2022Quote: ... The RT-qPCR reactions were performed using a Roche Diagnostics Lightcycler 2.0 (Roche, Indianapolis, IN), using a protocol previously described in detail42,63 ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the LightCycler®480 SYBR Green I Master-Ki (Roche): Primers were used for GAPDH (forward ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR mix was prepared using 1X Light Cycler 480 SYBR Green Master (Roche, Germany) and primer pairs at 5uM (Table 2) ...
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bioRxiv - Plant Biology 2023Quote: ... was used to perform the RT-qPCR on a Roche LightCycler 96 thermocycler (Roche, USA). The relative gene expression levels were calculated using the 2-ΔΔCT method as described (Livak and Schmittgen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT-qPCR was performed using a Roche LightCycler® 480 System (Roche Diagnostics, Rotkreuz, Switzerland) thermocycler using qPCR MasterMix No-ROX PyroTaq EvaGreen 5x for HRM (CMB-Bioline ...
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bioRxiv - Genetics 2022Quote: ... 10% of cleared lysate was set aside to serve as input samples and the remainder was incubated at 4°C with anti-GFP antibodies (Roche #11814460001) for 3 h under gentle rotation ...
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bioRxiv - Immunology 2019Quote: ... Individuals were considered to have AITD if they had a positive TPOAb titer (3 times more than the threshold set by the manufacturer [18 IU/mL for Abbott assay and 100 IU/mL for Roche assay]) or had a TSH level more than 10 mIU/L ...
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bioRxiv - Immunology 2021Quote: Single cells were sorted directly into PCR tubes containing 25 μl PCR lysis buffer (1x Buffer 1 from the Expand Long Template PCR System [Roche], 0.5 mg/ml Proteinase K) and lysed for 1h at 55°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche) and UCSC Genome browser (Table 2) ...
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bioRxiv - Plant Biology 2019Quote: ... 0.5 μM of both forward and reverse primers and 1 × FastStart SYBR Green Master mix (Roche). Steady state levels of transcripts were quantified using primers listed in Supplemental Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... we used gene-specific primers and Light Cycler® 480 SYBR Green I Master (Roche Diagnostics). Polymerase chain reaction conditions were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was amplified by primers in a 10µl reaction using KAPA SYBR FAST (Kapa Biosystems). We calculated mRNA using a ΔΔCT relative to the average of the housekeeping genes GAPDH expression ...
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bioRxiv - Neuroscience 2022Quote: ... Primers were designed using the Universal Probe Library (UPL) Probefinder software for human (Roche, version 2.53) or using previously published sequences and adjusted to be intron-spanning ...
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bioRxiv - Cell Biology 2022Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche). All samples were amplified in triplicates in a 384-well plate using the following cycling conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... gDNA was amplified using SV40_NLS_seq_f and SV40_NLS_seq_r (Supplementary Table 8) primers using Kapa Hifi (Kapa Biosystems KK2602) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the forward and the reverse primers and 2x KAPA HiFi Hot Start Ready Mix (Roche, CA). PCR was performed with the following protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time amplification was carried out using gene-specific primers and SYBR Green Master mix (Roche) on a Lightcycler LC480 apparatus (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5μL Reverse primer (5μM) and 5μL LightCycler® 480 SYBR Green I Master reagent (Roche Diagnostics) was performed and analysed on a Roche LightCycler® 480 System (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2021Quote: ... and reverse transcribed to cDNA using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). DYNLL1 was tested for splicing defects ...
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bioRxiv - Physiology 2020Quote: ... Eluted DNA was amplified with specific primers (Table S2) using SYBR Green Master (Roche, Basel, Switzerland), and the resulting signals were normalized to input activity.
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bioRxiv - Physiology 2022Quote: ... Primers were designed using Universal Probe Library Assay Design Center (Roche Applied Science, Burgess Hill, UK) or ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana using the primers as indicated in Supplemental Table S9 with high-fidelity Taq enzyme (Roche) and cloned into pGGA000 or pGGC000 GreenGate entry vector (Lampropoulos et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche) and UCSC Genome Bioinformatics (table 3) ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (1.5 μg) was reverse-transcribed using polyT primers and an Expand Reverse Transcriptase kit (Roche) according to the manufacturer‟s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The concentration of individual samples was confirmed using the KAPA Library Quantification Standards with Primer (Roche Sequencing Solutions Inc ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR was performed with specific primers (Table S2) and LightCycler 480 SYBR Green I Mastermix (Roche) using the LightCycler 480 II real-time PCR device (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Duplicates of cDNA samples and specific primers were mixed with Lightcycler SYBR Green I Master (Roche). Reactions were carried out on a Lightcycler 480 Instrument II (Roche ...
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bioRxiv - Immunology 2023Quote: ... All primer extensions and amplifications were performed using the KAPA Long Range HotStart Ready Mix (Roche). The resulting libraries underwent purification using KAPA HyperPure beads (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Duplicates of cDNA samples and specific primers were mixed with Lightcycler SYBR Green I Master (Roche). Reactions were carried out on a Lightcycler 480 Instrument II (Roche).
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bioRxiv - Microbiology 2024Quote: ... 2.5 μL of 5 μM reverse primer and 10 μL of KAPA SYBR FAST (KAPA Biosystems). The qRT-PCR was done in CFX96 Real-Time System C1000 Touch Thermal Cycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 4hrs and amplified with index primers using KAPA HiFi HotStart Uracil+ Ready Mix (KAPA Biosystems). The samples after PCR amplification were then purified using Ampure XP Beads (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were PCR-amplified during 5 cycles with Kapa HIFI PCR kit (Roche, Basel, Switzerland) before standard library quality control with standard sensitivity NGS Fragment kit (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with Power SYBR Green PCR Master Mix (Thermofischer) on a Lightcycler 96 (Roche) with the following primers ...
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bioRxiv - Systems Biology 2020Quote: ... One microliter of this suspension was used for colony PCR (KAPA2G Robust HotStart PCR Kit, Roche). Samples with a small amplicon size were considered positive hits ...
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bioRxiv - Systems Biology 2020Quote: ... One microliter of this suspension was used for colony PCR (KAPA2G Robust HotStart PCR Kit, Roche), whereby a ∼500-bp amplicon was amplified by a primer pair in which the 3’ end of one primer was complementary to the mutant allele ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed by adding 15µL PCR mix containing 0.5U KAPA HiFi HotStart (Kapa Biosystems), 1x KAPA Buffer (Kapa Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using the Expand High Fidelity PCR System dNTPack (Roche Diagnostics GmbH, Mannheim, Germany), with 4.5 mM MgCl2 and 3.5 U of enzyme mix used for each reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... One-step quantitative real-time PCR was performed on a Lightcycler 480 II PCR system (Roche) by using SYBR Green qPCR SuperMix (Bio-rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time quantitative PCR (qRT-PCR) reactions were performed using a Roche LightCycler (Roche, Grenzacherstrasse, Switzerland) and Roche SybrGreen Master Mix reagent (SybrGreen ...
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bioRxiv - Genomics 2020Quote: ... Both PCR steps were run on a real-time PCR instrument (LightCycler 480 II from Roche). DNA samples from Alu carriers (and negative controls ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Standard PCR-free libraries were prepared using KAPA Hyper PCR-free library reagents (KK8505, KAPA Biosystems). Total genomic DNA was sheared into fragments of approximately 200-600 base pairs (bp ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems) in 20 μl reactions ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems), 20 ng template and appropriate primers in 20 μl reactions ...