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Citations for Roche :
901 - 950 of 2089 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with blocking buffer (4 % skim milk in 1 × PBS) followed by incubation with the primary antibody: (monoclonal anti-GFP mice 1:5000 (Roche), rabbit anti-GFP 1:10000 ...
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bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-BALL (Gift from A. Herzig, IF 1:20, ChIP: 2μl) mouse anti-GFP (Roche, 11814460001, IF:1:50), mouse anti-Tubulin (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... eGFP-tagged readthrough reporter was immunoprecipitated by addition of 10 μg of mouse monoclonal anti-GFP IgG (Roche, cat#11814460001) or normal mouse IgG as a negative control (Proteintech ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were then probed for 16 h at 4 °C with the following primary antibodies in 3% (w/v) skim milk in PBS: mouse anti-GFP (1:1000, Roche), rabbit anti-SBP1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected DRG neurons were fixed 15 min in 4 % paraformaldehyde (PFA) and hybridized with DIG-labeled GFP cRNA probes (Roche) as described previously (Merianda et al. ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of the post-nuclear supernatant was saved as the input lysate and 450 μL was incubated with 5μg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with SP-RFP and either shRNA SP-GFP or empty vector (pCDNA3) using a lipofection method (X-tremeGENETM HP DNA Transfection Reagent, Roche) at a ratio 1:3 ...
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bioRxiv - Microbiology 2019Quote: ... Sendai Virus (SeV) and Modified vaccinia Ankara (MVA)-gfp viral stocks were extracted using the High Pure Viral Nucleic Acid Kit (Roche). When indicated ...
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bioRxiv - Cancer Biology 2019Quote: 851FH-GFP and 851FH-TCF21 CAFs were lysed in RIPA lysis buffer supplemented with EDTA-free protease inhibitor cocktail tablets (Roche) and normalized for total protein amount ...
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bioRxiv - Cell Biology 2020Quote: ... and SD-N (0 and 24 h) samples were TCA precipitated [21] and analyzed by Western blot with anti-GFP bodies (11814460001; Roche). All the experiments were done at least in duplicate.
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bioRxiv - Cell Biology 2022Quote: ... HEK 293 cells stably-expressing GFP-DFCP1 and RFP-ATG9 were generated by transfection of HEK 293 GFP-DFCP1 cell with RFP-ATG9 using X-tremeGENE 9 (Roche) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were hybridised with monoclonal antibodies against GFP followed by anti-mouse alkaline-phosphatase conjugated antibody and signals were developed using NBT-BCIP (Roche).
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bioRxiv - Molecular Biology 2022Quote: ... Sonicated samples were centrifuged and the supernatant (soluble fraction) was incubated for 1 h at 4°C with beads crosslinked to anti-GFP antibody (11814460001; Roche) or to M2 anti-FLAG antibody (F1804 ...
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bioRxiv - Plant Biology 2024Quote: ... and the immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with Abcam (Cambridge, UK) anti-GFP and anti-HA antibodies (Anti-HA High Affinity Roche 3F/10 ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng of DNA was analyzed by qPCR using primers specific for vector DNA (GFP) and genomic DNA (ADCK3) on a LightCycler 480 II (Roche). Viral genomes per cell were calculated by dividing total viral genomes (detected by GFP ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Microbiology 2023Quote: Cells transiently expressing M2-1-GFP or GFP as control were lysed with cell lysis buffer (CST) supplemented with complete mini-EDTA-free protease inhibitors (Roche) on ice and cell debris removed by centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... α-Ape1 (1:5000) (Hamasaki et al., 2003), α-Prb1 (laboratory stock, 1:5000) and α-GFP (Roche, 11814460001, 1:1000), were used as primary antibodies ...
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bioRxiv - Genetics 2023Quote: ... for 30 min at room temperature and incubated for 2 h at room temperature or overnight at 4°C with primary antibodies against GFP (Roche), androgen receptor (Invitrogen) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... A total of ∼1×107 HEK293T cells expressing GFP-STARD3 were lysed with 1 mL lysis buffer supplemented with a protease inhibitor cocktail (cOmplete, Roche). Lysates were clarified by centrifugation at 12000 g for 60 min and were mixed with the beads coated with 2 mg GST/GST-VAPA-MSP for 60 min at 4°C ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... a total of ∼1×107 HEK293T cells stably expressing the GFP-STARD3 or Flag-tagged YWHAH were harvested and lysed using the lysis buffer supplemented with protease inhibitor (Roche). Clarified lysate was mixed with pre-washed 10 𝜇l of GFP-Trap A beads (Chromotec gta-20 ...
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bioRxiv - Cancer Biology 2019Quote: ... Pellets were resuspended in 5 mL of membrane isolation buffer (5 mM TrisHCl pH 7.6, 5 mM MgCl2, 1 mM EGTA, protease inhibitor cocktail cOmplete [Roche Applied Science, Penzberg, Germany]) with a glass dounce tissue grinder (Wheaton ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgOAc, 5 %w/v glycerol, 0.25 %w/v NP-40, 3 mM DTT, 1 mM PMSF, Roche protease inhibitor cocktail (PIC), pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... pellets were resuspended in lysis buffer (50 mM HEPES pH 8, 400 mM NaCl, 75 mM Imidazole pH 8, 5% v/v glycerol, 5 mM 2-Mercaptoethanol, supplemented with Roche Protease Inhibitor Tablets) and lysed using an Emulsiflex-05 homogenizer ...
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bioRxiv - Molecular Biology 2019Quote: ... washed once with ice cold Phy Buffer (150 mM Potassium Acetate, 5 mM Magnesium Acetate, 20 mM HEPES-KOH pH 7.4, 5 mM DTT, protease inhibitor cocktail [Roche], 10 µg/mL cycloheximide). Cell pellets were then re-suspended in 1 mL Phy Buffer ...
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Tryptophan Metabolites And Their Predicted Microbial Sources In Fecal Samples Of Healthy IndividualsbioRxiv - Microbiology 2024Quote: ... Libraries were amplified for 13 PCR cycles in 50 μl reactions containing 150 pmol of P1.1 (5’-AATGATACGGCGACCACCGAGA) and P3 (5’-CAAGCAGAAGACGGCATACGAGA) primer and Kapa HiFi HotStart Library Amplification kit (Cat# kk2612, Roche Sequencing and Life Science). Library quantification and size estimation were performed using a Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of pefabloc (Roche, 5 mM final concentration) and then dialyzed into 10 mM Tris pH 7.5 with 2 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of pefabloc (Roche, 5 mM final concentration), then dialyzed into 10 mM tris(hydroxymethyl)aminomethane (Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol) containing 1×EDTA-free protease inhibitor cocktail (Roche), sonicated and centrifuged at 10,000 × g for 5 min twice ...
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bioRxiv - Immunology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5 mg/mL eComplete Mini protease inhibitor tablet (Roche). Lysates were sonicated for 10 seconds 8 times in an ice-cold water bath and centrifuged to separate the protein extract ...
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bioRxiv - Cell Biology 2019Quote: ... 5% glycerol) plus cOmplete protease inhibitor cocktail without EDTA (Roche). The suspension was clarified by a series of consecutive centrifugations (500 × g for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μl of KAPA2G Fast HotStart ReadyMix (KK5603, KAPA Biosystems) and 3.5 μl of sterile water were mixed in a 10 µl reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl RNase A (2.5 g, DNase-free, Roche #11119915001) or dH2O ...
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bioRxiv - Immunology 2019Quote: ... for 3 h followed by 5 mM ATP (10519987001, Roche) or 20 μM nigericin (N-7143 ...
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bioRxiv - Genetics 2020Quote: ... 5 µL of 2x Kapa library amplification mix (Roche, KK2702), 0.2 µL forward primer (10 µM ...
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bioRxiv - Neuroscience 2020Quote: ... and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, Roche) in NTMT pH9.5 solution (100mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, #11383221001, Roche) and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT ...
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bioRxiv - Biochemistry 2020Quote: ... 5 U/mL rabbit pyruvate kinase (Roche Diagnostics, Cat# 10128155001), 8 U/mL lactate dehydrogenase (Sigma-Aldrich)) ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM BME) including cOmplete EDTA-free protease inhibitor (Roche) and subjected to lysis by sonication ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM BME) including cOmplete EDTA-free protease inhibitor (Roche) and subjected to lysis by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg DNase I (from Bovine Pancreas grade II, Roche) was added to 50 mL of lysate ...
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bioRxiv - Microbiology 2020Quote: ... 5% Na deoxycholate) supplemented with protease/phosphatase inhibitor cocktail (Roche) and clarified from the nuclei by centrifugation at 13,000×g for 10 min at 4°C for quantitative western blot analysis (see below) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol and mini-complete EDTA-free protease inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM β-mercaptoethanol supplemented with protease inhibitor cocktail (Roche). Cell lysis was accomplished by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol and protease inhibitors (Complete Protease Inhibitor Cocktail, Roche). The lysate was centrifuged at 160,000 x g for 20 min ...