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Citations for Roche :
1001 - 1050 of 2089 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Thee injections of either an anti-GluA2 antibody (clone 15F1, gift from E. Gouaux) or a monoclonal anti-GFP IgG1-K (Roche, 11814460001) were targeted to the L2/3 of S1 (−0.1 to 0.3 mm dorsoventral) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral supernatants were prepared by transfecting 293T cells with GFP- or ABCG-targeting shRNAs encoded in pLKO.1 vectors using X-tremeGENE 360 transfection reagent (Roche, Germany) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... 10% of cleared lysate was set aside to serve as input samples and the remainder was incubated at 4°C with anti-GFP antibodies (Roche #11814460001) for 3 h under gentle rotation ...
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bioRxiv - Biochemistry 2019Quote: ... The transfection of GFP-RNase H1 R-loop-binding domain (GFP-HB) for R-loop detection into HEK293 cells was performed with the FuGENE Transfection reagent (Roche, E269A).
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bioRxiv - Plant Biology 2020Quote: ... by standard SDS-PAGE and detection by chemiluminescence with a monoclonal Anti-GFP antibody (Mouse IgG1K, clones 7.1 and 13.1, Roche Cat. No. 11814460001) and Anti-Mouse secondary antibody HRP-conjugated (Amersham ECL GE Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Total NUC/GEN polypeptide abundance was detected by probing duplicate blots with a mouse anti-GFP antibody (#11814460001; Sigma Aldrich (Roche), Darmstadt ...
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bioRxiv - Plant Biology 2023Quote: ... All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche; anti-D2 antibody from Agrisera). Coomassie brilliant blue (CBB ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% bovine serum albumin (BSA)/TBS-T ...
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bioRxiv - Cell Biology 2024Quote: HEK293T cells were seeded at 30 000/45 000 cells/cm2 and transfected 24 h after with plasmids (pcDNA3.1-GFP, MPV17-HA or MPV17) preincubated with XTremeGENE HP Transfection Reagent (Roche, Basel, Switzerland) for 30 min at room temperature in Opti-MEM I serum-free medium (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Immunostaining was performed after FISH development by re-blocking planarians in Blocking Solution (5% heat inactivated horse serum, 5% Roche Western Blocking Buffer in TNTx) for 2 hours at room temperature ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Genetics 2021Quote: ... extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D) coated with anti-GFP antibodies (Roche ref:11814460001). Proteins extracts were resolved on 4-15% polyacrylamide gels ...
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bioRxiv - Cell Biology 2021Quote: ... To determine the dimerization of sFeoFL::GFP and sFeoN:GFP a Western Blot was generated from a 4-12% gradient SDS and a Native polyacrylamide gel side-by-side using a mouse anti-GFP antibody (Roche Nr. 11814460001) at 1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: ... ChIPs were performed by combining sonicated chromatin (500 ng DNA content) with either 1 μg mouse mAb α-GFP (Roche Diagnostics #11814460001) or 1 μg rabbit α-PfHP1 12 in incubation buffer [(5% Triton-X-100 ...
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bioRxiv - Molecular Biology 2020Quote: ... The Dynabeads™ were washed once with 2 ml of PBS and coated with 15 μg of mouse monoclonal anti-GFP antibody (Roche Applied Science). The beads were finally washed three times with 2 ml of lysis buffer and added to the whole cell extract for a 2 h incubation with gentle rotation ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Molecular Biology 2023Quote: ... Co-purification of FlgP or PflD-sfGFP was investigated by western blot (WB) analysis using FlgP specific antisera (21) or an anti-GFP antibody (Roche #11814460001, RRID:AB_390913), respectively ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA) supplemented with phosphatase inhibitors (5 mM ß-glycerophosphate, 5 mM NaF, 1 mM Na3VO4) and protease inhibitors (Roche cOmplete ULTRA Tablets, EDTA-free). The lysates were sonicated on ice (4x 10s bursts ...
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bioRxiv - Biophysics 2021Quote: ... 5 μL FastStart SYBR Green Master (Roche Diagnostics, Indianapolis, IN USAl), 0.4 μL qRT-PCR primers (Table S2) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.3 μl of 5 U/μl FastStart Taq DNA polymerase (Roche), 0.4 μl of 100 μM “up” or “down” barcode primer pair ...
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bioRxiv - Microbiology 2019Quote: ... or 5 μl RNA molecular weight marker I DIG-labelled (Roche) were treated in the same way as the RNA samples to allow the detected transcript length to be estimated ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol: pH 7.4) containing protease and phosphatase inhibitor cocktails (Roche) for 30 min at 4°C with intermittent mixing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 % DMSO and 12.5 μL of KAPA HiFi HotStart ReadyMix (Roche). The parameters for PCR were 95 °C for 5 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 % DMSO and 12.5 μL of KAPA HiFi HotStart ReadyMix (Roche). The parameters for PCR were 95 °C for 5 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 % DMSO and 12.5 μL of KAPA HiFi HotStart ReadyMix (Roche). The primers used to amplify the library were P1 (5’-GGGGAAGCTTGCCGTAAG-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... and .5 mM spermidine along with phosphatase and protease inhibitors (Roche). All steps were performed on ice ...
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bioRxiv - Neuroscience 2021Quote: ... and 5’-GCTTGAGGTAGC CCTGTTGTCACC-3’ using KAPA HiFi Hotstart polymerase (Roche:KK2602). This PCR reaction produced a 185 bp wild type band and a 750 bp knockout band in heterozygous animals ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μl 2500 U/ml DNase I (04716728001 Roche, Sigma Aldrich) was added to the single-cell suspension 10 min before the end of the digestion ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM CaCl2 and 15 units of S7 micrococcal nuclease (Roche). Lysates were sonicated for 10 seconds at low power followed by incubation on ice for 30 minutes and clarification by centrifugation at 13,000 x g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol) containing 1× complete EDTA-free protease inhibitor cocktail (Roche). Insoluble debris was removed by centrifugation at 15000 rpm for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... ATP 5 mM) supplemented with proteases inhibitors (cOmplete EDTA-free, Roche). Cells were homogenized in a ball homogenizer with 10 μm clearance ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% (w/v) glycerol) with protease inhibitor tablet (EDTA-free, Roche), and lysed by sonication ...
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bioRxiv - Developmental Biology 2022Quote: ... and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche). Images were acquired by the Leica MZ10F microscope with the DS-Ri1 camera.
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bioRxiv - Cell Biology 2021Quote: ... and digested with collagenase D (5 mg/mL, #11088882001, Roche, Germany) and dispase (2 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... pastoris were removed by incubation with 5 mU of EndoglycosidaseH (Roche) in 50 mM sodium citrate buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... 5–10 mM NaF) containing a protease inhibitor cocktail (Roche, 04693132001) and the protein lysis buffers were incubated on ice for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... PCR conditions were as follows: 5 μl Kapa Enhancer (Kapa Biosystems), 5 μl Kapa Buffer A ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 2) 5 10^6 copies of bacteriophage MS2 RNA (Roche) were spiked in per isolation ...
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bioRxiv - Neuroscience 2019Quote: ... 5 mM DTT and protease inhibitors (#04 693 159 001 Roche). After a squeezing step ...
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bioRxiv - Genomics 2019Quote: ... 0.08U KAPA2G Robust HotStart DNA Polymerase (5 U/μL, Roche KK5517). PCR reactions were performed using a thermocycler with the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The PVDF membranes were blocked in 5% Western Blocking Reagent (Roche) then probed with primary and secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μl FastStart Universal SYBR Green Master mix (Roche, Basel, Switzerland) and 3 μl MilliQ ...
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bioRxiv - Neuroscience 2020Quote: ... 5 mM magnesium acetate) supplemented with protease/phosphatase inhibitor cocktail (Roche) and RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... 5% (w/v) glycerol] supplemented with complete protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail (Sigma ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated in 5 mM 1,4-dithiothreitol (Roche, Basel, Switzerland) for 20 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM Benzamidine and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) was added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μl of 10X 5-Bromo-2’-deoxyuridine (BrdU) (Roche, Germany) per well was added ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT) supplemented with EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication for ten cycles of 30 pulses of sonication and 30 s breaks using a Hielscher UP200st set to pulse=70% ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µM MG132) and a protease inhibitor cocktail (Roche, Basel, Switzerland), as previously described72 ...