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Citations for Roche :
851 - 900 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
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bioRxiv - Molecular Biology 2019Quote: ... precleared with protein G-agarose beads (Roche), and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein G agarose beads (Roche 11243233001) were prepared ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were cleaved by adding Trypsin (Roche) in a 1:100 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 x protein inhibitors cocktail tablet (Roche), 2000 units of RNasin Plus ribonuclease inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... was coupled to protein A beads (Roche). Immobilized antibody was incubated with peptide mixtures ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A Agarose (Roche) were washed and equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to PVDF membranes (Roche) in tris-glycine buffer using semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μl of Protein-A beads (Roche) was added to lysates and incubated with rotation for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell survival relative to vehicle-treated cells was assessed using the WST-1 kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: The cell proliferation assay was carried out using WST-1 Cell Proliferation Assay Kit (Roche). Transfected cells were seeded into 96-well plates at a density of 1 × 103 cells/well ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin RNA probes were synthesized using the T3/T7 Transcription Kit (Roche; Supp. Table 1). Larvae were collected ...
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bioRxiv - Genomics 2021Quote: ... rRNA was depleted from 1 μg of total RNA with the RiboErase kit (KAPA Biosystems). Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... RNA (1 µg) libraries were constructed using 1llumina TruSeq Stranded mRNA Library Prep Kit (ROCHE) followed by bead size selection (~280 bp ...
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bioRxiv - Immunology 2022Quote: ... and the OptiView DAB Detection Kit for the PD-1 IHC (Ref: 06396500001, Roche Ventana) were used as detection system ...
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bioRxiv - Bioengineering 2023Quote: ... High Pure RNA Isolation Kit and Cell Proliferation Reagent WST-1 were purchased from Roche. QIAshredder and RNeasy Fibrous Tissue Mini Kit were purchased from Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was incubated for o/n with 40μl of anti-HA agarose beads (rat anti-HA, 3F10 Roche) at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was incubated with horseradish peroxidase (HRP)-conjugated rat anti-HA (clone 3F10) monoclonal antibodies (Roche, Indianapolis, IN) at a dilution of 1:5,000 for 2 hours ...
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bioRxiv - Neuroscience 2019Quote: ... some TK rats (n=11) were orally administered 4 mg of valganciclovir (val) to reduce neurogenesis (Hoffman La-Roche; delivered in 0.5 g peanut butter + chow pellets ...
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bioRxiv - Neuroscience 2022Quote: ... All co-cultures were fixed and post-fixed (before and after incubation with primary rat anti-HA antibody (Roche), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... UK). Rat anti-HA mAb (3F10) and rabbit anti-GFP pAb (code No. 598) were from Roche (Basel, Switzerland) and Medical and Biological Laboratories (Aichi ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with rat anti-HA antibody (clone 3F10, Roche), or rabbit anti-TgSkp1 affinity purified polyclonal antibody UOK75 [76],
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed with gene specific p rimers (see Key Resource Table) using Sybr green detection on a Light Cycler 480 (Roche) or Quantstudio6 (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate qPCR reactions of the Telomeric (T) product and the Single copy gene (S) (HBB) were amplified using a Roche LightCycler 480 II (Roche) using the following programs ...
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bioRxiv - Cell Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The relative mRNA expression level of each target gene was quantified by qPCR using KAPA SYBR fast qPCR master mix (Roche). Data were normalized to GAPDH and expressed relative to control unstimulated macrophages ...
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bioRxiv - Developmental Biology 2021Quote: ... for genes of interest and Gapdh (a normalization control for amplification efficiency) on a LightCycler 480 Real-Time PCR machine (Roche). The primer sequence used for qPCR analysis is listed in Supplementary Table 12 ...
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression signals were visualized using nitroblue tetrazolium/5-bromo-4-chloro-3-indolylphosphate solutions using a standard method (Roche). Whole-mount and sectioned specimens of Ciona were observed using an SZX12 stereo microscope (Olympus ...
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bioRxiv - Genomics 2020Quote: ... approximately 3,000 individuals were collected from the wild and cleaned as described above and homogenized using BioMasher II (Nippi) in PBS (Nippon Gene) on ice with protease inhibitors (Roche). The lysate was heated at 92°C for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Real time PCR for tuft cell markers and antimicrobial peptide genes was performed using Fast Start Universal SYBR Green Master (Roche) and ViiA™7 Real-Time PCR System (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed to determine relative gene expression in pigmented versus non-pigmented mouse melanocytes using a LightCycler 480 instrument (Roche) with PrimeTime Gene Expression Master Mix (IDT DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... forward and reverse primers for the genes of interest (see Supplemental table S2) were mixed with FastStart Universal SYBR Green Master (Rox; Roche). 5 µl of this mixture was pipetted in duplicate per condition in a 384 well plate ...
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bioRxiv - Microbiology 2020Quote: ... was used for each time point (mid-log and stationary) along with gene-specific primer and SYBR Green master mix (Roche) in a 10 μL reaction according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Target enrichment for next-generation sequencing was performed with the UCSF500 Cancer Gene Panel (developed by the UCSF Clinical Cancer Genomics Laboratory; Roche) or the SeqCap EZ Exome + UTR library probes (Roche #06740294001) ...
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bioRxiv - Microbiology 2019Quote: ... The two primers and probe (u-16S) used to detect the 16S rRNA gene were designed with the software LC probe design2 (Roche). 16S rRNA sequences of known pathogenic or commensal species of the respiratory tract were aligned and a final selection of primers and probe was accomplished according to their universality (Figure S1) ...
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bioRxiv - Developmental Biology 2019Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences are shown in Table S3) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
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bioRxiv - Genomics 2019Quote: ... Primers and probes were designed using the predicted genes sequences generated in the RNA-seq data analysis and the Assay Design Center from Roche, selecting “Other organism” (https://lifescience.roche.com/en_gb/brands/universal-probe-library.html) ...
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bioRxiv - Immunology 2021Quote: ... Relative gene expression of RORγt and FOXP3 was performed using Real Time PCR (Lightcycler 480, Roche Molecular System, Pleasanton, CA), SYBR Green and primers specific to RORγt ...
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bioRxiv - Neuroscience 2020Quote: ... Real time quantitative PCR was performed on the E gene as described recently (Corman et al., 2020) on a Lightcycler LC480 (Roche).
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bioRxiv - Cancer Biology 2020Quote: ... were co-transfected with 2ug of the Cas9/sgRNA vector PX458 and 4 ul of ssODN HDR template (20 uM) using Xtreme gene (Roche). Two days following transfection ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed with sigA/Rv2703 gene as control and fold change was measured by ΔΔCt method using Fast Start universal master mix (Roche). The following primers were used ...
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bioRxiv - Microbiology 2019Quote: ... and the ketoreductase-B domain of the mycolactone polyketide synthase genes (KR-B) [22] using RT-PCR reagents from Roche PCR Kit (Roche Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... Université libre de Bruxelles, Belgium) expressing a Wnt reporter gene (super top flash, STF) with X-tremeGENE HP DNA Transfection Reagent (Roche). The luciferase activity was detected 48 hours after transfection using the luciferase assay system (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... along with packaging plasmids including psPAX2 and pMD2.G (from Dr. Guang Hu in NIH) using the X-treme GENE 9 transfection reagent (Roche). Viral supernatant was collected and cellular debris was removed by syringe filtering (0.45 μm pore size ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was done in triplicate with PerfeCTa qPCR SuperMix Low ROX (Quanta Biosciences) with gene specific probes (Universal Probe Library, UPL, Roche) and primers ...
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bioRxiv - Neuroscience 2021Quote: ... Specific primers for the different genes of interest were designed using either the Universal ProbeLibrary (ProbeFinder version 2.5 for mouse, Roche Diagnostics) or PrimerBlast (NCBI ...
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bioRxiv - Immunology 2020Quote: ... cells were cotransfected with both the reporter gene and one of the different putative transcription factors using X-tremeGENE 9 (Roche) following the manufacturer’s recommendations ...
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bioRxiv - Pathology 2019Quote: qRT-PCR was performed to measure the mRNA expression level of the endogenous PDS genes using the SYBR Green I Master (Roche) in either N ...
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bioRxiv - Plant Biology 2019Quote: ... Blots were hybridized with a digoxygenin (DIG)-labelled specific DNA probe for the HYG gene and signals were detected using CDP-Star according to the manufacturer’s instructions (Roche Diagnostics)