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Citations for Roche :
701 - 750 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Quantitative gene expression analysis was performed using the LightCycler®480 SYBR Green I Master (Roche) and measured using the LightCycler®480 Instrument II (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression levels of the respective genes of interest were measured using LightCycler 480 II system (Roche). The amounts of the respective mRNAs were normalized relative to those of 18S ribosomal RNA ...
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bioRxiv - Genetics 2023Quote: ... or TaqMan gene expression assays (see details below) on the LightCycler 480 (Roche Diagnostics, Mannheim, Germany). Human tissue was obtained from the NIH NeuroBioBank’s Brain and Tissue repository at the University of Maryland ...
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bioRxiv - Neuroscience 2023Quote: ... and two sets of primers for WPRE or hGHpA genes using LightCycler 480 (Roche, Basel, Switzerland). The final titration of the AAV was estimated as relative quantitation according to a calibration curve calculated from the known numbers of copies of AAV plasmids.
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bioRxiv - Immunology 2023Quote: ... prior to gene expression analysis by real-time quantitative PCR (LightCycler, Roche; or QuantStudio 3, ThermoFisher). Quantitect SYBR green PCR mastermix (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR for each candidate gene was performed using LightCycler 480 SYBR Green I Master (Roche) with the following cycle conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche). PCR amplification was performed as follows ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability assay was performed using a WST-1 assay kit (Roche). Briefly ...
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bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Developmental Biology 2019Quote: ... and protein G agarose (Roche). Immunoprecipitates were analyzed on SDS-PAGE gel bands ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein A Agarose (Roche 11134515001), blocked with 10μg BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein-A agarose beads (Roche) washed three times with lysis buffer were mixed with IPed lysates and incubated at 4 °C for two hours or overnight with constant rotation ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and protein-G-agarose (Roche). Oligonucleotide complexes were labelled with alpha-32P dCTP using terminal deoxynucleotidyl transferase (Fermentas ...
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bioRxiv - Immunology 2019Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein-G agarose beads (Roche) were then added ...
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bioRxiv - Immunology 2020Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... protein G agarose beads (Roche) were added to the cell lysate and incubated for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein A-Agarose (Roche, 11134515001) and Protein G-Agarose (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose beads (Roche) were pre-incubated with 1 µg of indicated primary antibody for 2 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitated proteins were immunoblotted for GFP- or HA-epitope tagged ubiquitin with antibodies for GFP (1:3,000, Roche #11814460001) or HA (1:4,000 ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM ATP (+ 2 mM DTT for Strep-KaiC36803 proteins)] including protease inhibitors (protease inhibitor cocktail, Roche or Nacalai). Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
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bioRxiv - Plant Biology 2022Quote: ... and the interaction between AtRsmD and the selected proteins was determined by immunoblot analysis with a mouse anti-GFP monoclonal antibody (1:5,000; Roche) and a mouse anti-Myc monoclonal antibody (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2023Quote: ... target proteins were detected by using primary antibodies of mouse monoclonal anti-GFP antibody (1: 2000; Roche, Cat. 11814460001), mouse anti-mCherry (1:5000 ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Cell Biology 2020Quote: ... HA-Kir6.2 was detected with HRP-conjugated rat anti-HA antibody (Roche, 3F10, 0.005 μg/ml). GFP was detected with mouse anti-GFP antibodies (AbCam ...
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bioRxiv - Neuroscience 2021Quote: ... Post hoc immunostaining was performed using rat anti-HA primary antibodies (Roche, #11 867 423 001) and anti-rat Alexa568 (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: Commercially available primary antibodies used for western blot are rat anti-HA tag (clone 3F10; Roche), rabbit anti-Cyclin B1 (4138S ...
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bioRxiv - Biophysics 2021Quote: ... Monoclonal cell lines were identified by immunocytochemistry using a rat anti-HA antibody (Roche Applied Science). HEK293 cells stably overexpressing rat sACt were as described (58).
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bioRxiv - Immunology 2022Quote: Stock solutions of collagen were prepared by dissolving 10 mg collagen I (rat tail-derived; Roche) in 3.3 mL of 0.2% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were probed with the following primary antibodies: rat α-HA (clone 3F10; Roche Applied Sciences), rabbit α-Myc (Cell Signaling Technology) ...
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bioRxiv - Plant Biology 2023Quote: ... the membranes were incubated with anti-HA-peroxidase high-affinity monoclonal rat antibody (clone 3F10; Roche) 1:1000 diluted in 2.5 % (w/v ...
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bioRxiv - Microbiology 2024Quote: ... were stained with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche). Alternatively ...
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bioRxiv - Cell Biology 2019Quote: ... and levels of p24 were determined by ELISA as previously described (65) The SEAP levels of the supernatants were determined by a chemiluminescent method (Roche) and were used to normalize the differences in transfection efficiencies.
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bioRxiv - Immunology 2020Quote: ... To quantify NETs we measured HNE-DNA complexes using ELISA specific for HNE and anti-DNA Ab of Cell Death Detection ELISAPLUS (Roche). Briefly ...
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bioRxiv - Biochemistry 2020Quote: RT activity was measured using an ELISA-based colorimetric reverse transcriptase activity assay (Catalog No. 11468120910, Roche Diagnostics, Indianapolis, IN). The HEPES integration buffer was used for the reaction ...
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Repeated phenotypic selection for cuticular blackness of armyworm larvae decreased stress resistancebioRxiv - Physiology 2019Quote: ... qPCR analysis of each gene was carried out using a Light-Cycler 1.3 instrument (Roche Applied Science) under the following conditions ...
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bioRxiv - Systems Biology 2020Quote: ... Metagenomes were obtained by pyrosequencing fragments of the 16S rRNA gene on the GS FLX system (Roche). Then the data were processed by replicating the same bioinformatics workflow followed by Amir and colleagues 21 that was described in the previous data description (Amir3) ...
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bioRxiv - Molecular Biology 2019Quote: ... The levels were normalized to two housekeeping genes (Actb, Gapdh) amplified with SYBR Green Master Mix (Roche), as reported before (22).
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 15 minutes with 4 μL of X-treme Gene HP DNA transfection reagent (Roche). Transfected cells were selected for by hygromycin resistance ...
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bioRxiv - Plant Biology 2020Quote: ... Melting data were analyzed using the Melt Curve Genotyping and Gene Scanning Software (Roche Life Science, Germany). Analysis of HRM variants was based on differences in the shape of the melting curves and in Tm values.
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bioRxiv - Genetics 2022Quote: ... Analysis of gene expression was performed using a The LightCycler® 480 real-time PCR System (Roche). The gene ACT1 was used as endogenous control ...
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bioRxiv - Plant Biology 2020Quote: ... Gene primers and probes were designed using Universal Probe Library (UPL) assay design centre (Roche Diagnostics Ltd.). RT-qPCR was performed using FastStart Universal Probe Master Mix (Roche ...
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bioRxiv - Genetics 2021Quote: ... which was performed with LightCycler 480 SYBR Green І Master Gene Expression Assays (Roche Molecular BiocheMHcals, Germany), is quantitated for the expression of β-actin/Akt/mTOR/p70S6K/eIF4E by employing the 2−ΔΔCT value models [31] ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments targeting the clpV gene were amplified from genomic DNA using KAPA HiFi DNA polymerase (Roche). Primers were designed from conserved regions identified by comparison of the clpV gene from the available K ...
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bioRxiv - Plant Biology 2022Quote: Gene expression was determined by quantitative real-time PCR (qRT-PCR; LightCycler 480, Roche Diagnostics, Rotkreuz, Switzerland) using SYBR Premix Ex Taq™ (TaKaRa ...
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bioRxiv - Neuroscience 2023Quote: ... with a two-step cycling protocol was used for the gene expression analysis using LightCycler 480 (Roche). Using the comparative ΔCt method (ΔΔCT method) ...
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bioRxiv - Cell Biology 2022Quote: ... and qRT-PCR was measured using gene-specific primers with FastStart SYBR Green PCR Master Mix (Roche) on a StepOne Real-Time PCR System (ThermoFisher) ...