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Citations for Roche :
1001 - 1050 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Digoxigenin-labelled sense and antisense RNA probes were prepared by in vitro transcriptions using recombinant plasmids of target genes made as mentioned above (Roche Life Science) and used for in situ hybridization ...
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bioRxiv - Microbiology 2021Quote: ... The Göttingen Genomics Laboratory determined the sequences of the partial 16S rRNA gene and transcript amplicons by using a 454 GS-FLX sequencer and titanium chemistry as recommended by the manufacturer (Roche, Mannheim, Germany).
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bioRxiv - Microbiology 2020Quote: ... These mutant viruses were made using HEK-293/17 cells that were transfected with 6 μg of viral DNA using Xtreme-GENE HP (Roche, Basel, Switzerland) Transfection reagent ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into HeLa cells growing on 100 mm dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols and processed for TEM at 18 hours post transfection ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR was performed using UBC and EEF as reference genes on a Roche Lightcycler 480 device (Roche Molecular Systems Inc.) with SYBR Green I Master kit (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... genes was performed using reagents and equipment supplied by Advanced Cell Diagnostics (ACDBio, Hayward, CA) and Ventana Medical Systems (Roche, Tuscon, AZ). The in situ hybridization RNAscope® probes where designed based on the simian HAV Macaca/Germany/2021 ...
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bioRxiv - Microbiology 2023Quote: ... we amplified the V3-V4 hypervariable region of the 16S rRNA gene via PCR with Hifi Hotstart Readymix (Kapa Biosystems; Boston, MA), forward primer 5’-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3’ ...
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bioRxiv - Plant Biology 2023Quote: ... Starting amounts for transcript levels were calculated based on the titration curve for each target gene using the LightCycler 480 Software (Roche; version 1.5.1.62). Two technical replicates were used and averaged in analyses for each biological replicate ...
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bioRxiv - Cell Biology 2024Quote: ... The expression levels of individual genes of interest were determined by quantitative real time PCR (qPCR, Roche Diagnostics, Germany or Stratagen, USA), primer pairs used for amplification are listed in Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... For gene expression analysis, appropriate primers (metabion international AG, Germany) and LightCycler® 480 SYBR® Green I Master (Roche, Mannheim, Germany) were used ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed using gene-specific primers (Supplemental Table S3) using Promega Go Taq® qPCR Master Mix on a Light Cycler 480 II® (Roche) thermocycler ...
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bioRxiv - Neuroscience 2024Quote: ... Antisense riboprobes against target genes were synthesized from 5 μg linearized plasmids using digoxigenin-(DIG) or fluorescein-labeled uridylyltransferase (UTP) (#11685619910, #11277073910, Roche, Mannheim, Germany) and the MAXIscript in vitro Transcription Kit (#AM1312 ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA levels were determined using gene-specific primers by SYBR green nucleic acid labeling (Selleckchem PCR Mastermix, #B21202) in a Lightcycler 480 (Roche, Indianapolis, IN). Fold change was calculated using the delta delta C(t ...
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bioRxiv - Immunology 2024Quote: 293T cells were co-transfected with plasmids encoding cognate heavy and light chains using the Xtreme Gene 9 DNA transfection reagent (Roche, Basel, Switzerland), then grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μl of slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μL of a slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were detected using ECL lumi-light plus (Roche) and images acquired using camera system (Chemitouch Biorad).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to nitrocellulose or PVDF membranes (Roche) in tris-glycine buffer at 110 V for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and protein extracted with ELB buffer containing PhosSTOP (Roche) and 50 μM sodium fluoride ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Immunology 2019Quote: Proteins were digested on beads using trypsin (Roche, Germany) in 6 M Urea ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 80V for 1.5 hours ...
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bioRxiv - Biochemistry 2019Quote: ... and then incubated with protein A–agarose beads (Roche) for 4 h ...
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bioRxiv - Genomics 2019Quote: ... protease inhibitors) and precleared with protein A beads (Roche) at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitations were performed using Protein A agarose beads (Roche). DivJ was immunoprecipitated using an anti-DivJ antiserum (15) ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were semi-dry blotted onto PVDF membranes (Roche), which were subsequently blocked in 5 % milk in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with Protein G beads (Roche, 1124323301) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 30V for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Neuroscience 2021Quote: ... Then homogeneous protein G-agarose suspension was added (Roche) to the suspension ...
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bioRxiv - Pathology 2019Quote: ... Proteins were detected using polyclonal antibodies against GFP (Roche), Histone H3 (GeneTex ...
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bioRxiv - Plant Biology 2021Quote: ... coupled to 50µl bed volume Protein A agarose (Roche) per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT and 1x protein inhibitor cocktail (Roche), and incubated at 25ºC for 1 h on a rotating wheel ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with protein G-agarose beads (Roche) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred onto PVDF membranes (Roche, Basel, Switzerland), and Western blot detection was carried out as previously described [32,33] ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Cancer Biology 2023Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Roche), nonspecific binding was blocked by incubation with 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... 400 µg of protein were reduced using DTT (Roche, Cat#10708984001 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with protein A agarose beads (Roche, #11134515001) at 4 °C overnight with shaking ...
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bioRxiv - Genomics 2020Quote: ... 2 g of DUX4 expression constructs were transfected into RD cells with 6 L of the X-treme GENE 9 DNA transfection reagent (cat. XTG9-RO, Roche Diagnostics, Indianapolis, USA) diluted in 100 L of Opti-MEM I (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... that amplify the coding sequence of the phytoene desaturase gene (tetur01g11270) (Table S1. PCRs were performed using the Expand Long Range dNTP Pack (Roche/Sigma-Aldrich, Belgium). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... HCMV glycoprotein B gene (gB) in the DNA sample was amplified in Lightcycler 480 real time PCR platform (Roche Molecular System, Pleasanton, CA) using TaqMan probe (LightCycler TaqMan Master ...
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bioRxiv - Microbiology 2021Quote: 16S rRNA gene concentrations in purified genomic DNA extracts were quantified using SYBR Green I Master on a LightCycler 480 II (Roche Molecular Systems, Inc.). The primer pairs for Bacteria and Archaea were Bac908F_mod (5’ s-AAC TCA AAK GAA TTG ACG GG-3′ ...
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bioRxiv - Neuroscience 2021Quote: ... For each gene the specific forward and reverse primers and probe were combined with Fast Start Universal Probe Master (Rox) Mastermix (Roche Diagnostics GmbH, Germany) and RNase free H2O (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: The threshold cycle of each target and reference gene amplification in control and mutant embryos was determined automatically by LightCycler® 96 Software (Roche Diagnostics, USA). Fold change in mutants versus controls was calculated with delta-delta-C(t ...
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bioRxiv - Microbiology 2023Quote: The absolute abundance of the 16S rRNA gene was measured on LightCycler® Roche 480 Real-time PCR instrument (Roche Inc., Basel, Switzerland). A total of 20 μL reaction system consisted of 10 μL LightCycler® 480 SYBR Green I Master Mix (2 × ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified DNA was quantified using GeneAce SYBR qPCR Mix α (NIPPON GENE, Tokyo, Japan) combined with a LightCycler 96 Real-Time PCR system (Roche, Basel, Switzerland). The primers used are listed in supplemental table (Table ...
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bioRxiv - Plant Biology 2023Quote: ... Southern blotting was performed with the digoxigenin (DIG)-labelled PCR-amplified gene fragment of HPT probe as described in the DIG System Manual (Roche, Inc., Basel, Switzerland). Genomic DNA (20 μg ...