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Citations for Roche :
851 - 900 of 7383 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Pellets were harvested after 16-18h and resuspended in lysis buffer (50 mM Tris-HCl, 500 mM NaCl, 5% glycerol, 1 tablets per 50 ml protease inhibitor (EDTA-free, Roche), 0.2 mM PMSF ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 24 hours in the presence of SARS-CoV-2 specific MPs [1 μg/mL] or 5 μg/mL phytohemagglutinin (PHA, Roche) in 96-wells U-bottom plates at 1×106 PBMCs per well ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5% glycerol with the addition of 1 EDTA-free cOmplete protease inhibitor cocktail tablet per 50 mL lysate (Roche). Cells were lysed by sonication on ice with stirring and the lysates were clarified by centrifugation at 20,000 g for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... digested using 0.1 % papain at 37°C for 10 min and for 5 additional minutes in presence of DNAse1 (1 mg/ml, Roche). After stopping papain activity by adding MC+ media ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM NaCl, 50 mM sodium pyrophosphate, 50 mM sodium fluoride, 1% Triton-X-100, 10% glycerol, 5 mM EDTA, Roche MiniProtease Inhibitor cocktail tablet ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with SAE2-T2A-SAE1 cDNA in the pcDNA5/FRT/TO vector and the Flp-recombinase cDNA in the pOG44 vector at a 5:1 pcDNA5/FRT/TO DNA: pOGG44 DNA ratio using FuGene6 (Roche) at a ratio of 4:1 FuGene (μl) ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... tumor tissue was dissected into approximately 1– 5 mm3 fragments and digested with collagenase Type D (2 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 5 mM EGTA, 1 mM DTT, 2 mM MgCl, and one EDTA-free protease inhibitor cocktail tablet; Roche) and sonicated in an ice bath for 6 minutes ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed 2x with 1x PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1x PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Cancer Biology 2023Quote: Tissues from animals were homogenized and lysed in HED buffer (25 mM HEPES, 5 mM EDTA, 10% glycerol, 1 mM DTT, and 1x cOmplete protease inhibitor [Roche]). Lysates were sonicated for 20 minutes in a water bath sonicator and cleared by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.0, incubated in a hybridization buffer [(50% formamide, 5× SSPE, 0.1% SDS, and 1 mg/ml Yeast tRNA (Roche Diagnostics)] containing the Avidin solution (Vector Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed 2 × 5 min at 4 °C with cold 1 × PBS containing protease inhibitor cocktail (Roche, #4693132001), snap frozen and stored at −80°C until awaiting further processing ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Lobes were separated into clusters of 1-5 oocytes and the follicular layer was removed i) enzymatically with Liberase (Roche) and ii ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 5 mL of either test buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and one protease inhibitor tablet (Roche) was then to resuspend the fine powder ...
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...
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bioRxiv - Immunology 2024Quote: The ear skin was minced finely with scissors and digested in RPMI1640 medium containing 333-666 µg/ml Liberase TL (1 vial with 5–10 mg pack size; Roche), 2 kU/ml DNase I (Merk Sigma-Aldrich) ...
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bioRxiv - Genetics 2020Quote: ... 0.25% deoxycholic acid) plus protease inhibitor and phosphatase inhibitor Data Setts (Roche Applied Science). 11 μg protein was separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and free fatty acids in plasma was performed using commercial kits (Biovision, Roche, Cayman). Mouse plasma lipoprotein profiles were established by TNO Biosciences (Leiden ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with phenylmethanesulfonyl fluoride solution and a protease inhibitor cocktail with ethylenediaminetetraacetic acid (Roche). Brain tissue was homogenized in ice-cold RIPA buffer (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated (nucleic acid purification on the MagNA Pure 96; Roche Life Science), reverse transcribed and Taqman PCR (on the 7500 RealTime PCR system ...
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bioRxiv - Microbiology 2021Quote: RNA from virus was extracted using High Pure Viral Nucleic acid extraction kit (Roche). Using gene specific reverse primers of target genes ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from samples using Magnapure LC total nucleic acid isolation kit (Roche). RNA amplification and quantification were carried out using a 7500 Real-Time PCR System (Applied biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... NEFA were analysed using the Free Fatty Acid Kit (half-micro test) (11383175001, Roche) and TG was measured using an enzymatic assay (DF69A ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasma concentrations of free fatty acid and glycerol were analyzed with reagents from Roche and Sigma ...
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bioRxiv - Microbiology 2020Quote: ... or using a manual protocol with the High Pure Viral Nucleic Acid kit (Roche Molecular Systems Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... The membrane was air dried and then blocked with nucleic acid blocking reagent (Roche) in PBS for 1hr and then incubated with 1:1000 Streptavidin AP (Roche ...
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bioRxiv - Microbiology 2021Quote: ... and ADNaCl (0.5% fatty acid free BSA from Roche, 0.2% dextrose and 0.85% NaCl) or in Middlebrook 7H10 supplemented with 0.5% glycerol and 10% oleic acid-albumin-dextrose-catalase (OADC) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was isolated (nucleic acid purification on the MagNA Pure 96; Roche Life Science), reverse transcribed and Taqman PCR (on the 7500 RealTime PCR system ...
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bioRxiv - Microbiology 2024Quote: ... total nucleic acids (NA) were extracted with the automated MagNA Pure LC instrument (Roche) using the MagNA Pure LC DNA Isolation Kit – High performance (Roche ...
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bioRxiv - Biophysics 2023Quote: ... The lysate supernatant was mixed with 200 µL of Ni-nitrilotriacetic acid resin (Roche Complete His-Tag purification resin ...
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bioRxiv - Systems Biology 2023Quote: ... DNA was extracted and purified using the MagNA Pure nucleic acid purification platform (Roche Diagnostics Corp. ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.0 with 0.75 M 6-aminocaproic acid) with freshly added protease inhibitors (Roche). After resuspension ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μg of trypsin (Roche, Switzerland) was added and the mixture was further incubated at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 5 μl proteinase K (Roche) in 20 mg/mL stock was added and incubation was continued at 55°C for 1 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μg/ml inorganic pyrophosphatase (Roche), and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase ...
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bioRxiv - Immunology 2021Quote: ... DNAse I (5 µg/ml, Roche) and 10% FBS (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 μg/mL DNaseI (Roche). For cell lysis ...
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bioRxiv - Genomics 2024Quote: ... 5 U Klenow DNA polymerase (Roche), and incubated for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 mg/ml collagen (Roche, 10103578001), and 15 U/ml Dispase II (Stemcell Technologies ...