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Citations for Roche :
801 - 850 of 7383 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Bioengineering 2023Quote: Liver tissue was lysed using RIPA buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, cOmplete® Mini Protease Inhibitor Cocktail [Roche Diagnosis ...
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bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 500 mM NaCl, 0.4% [wt/vol] SDS, 5 mM EDTA, 1 mM DTT and 1x cOmplete Protease inhibitor cocktail, Roche) for 20 min on a rocker at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mM Tris-HCl [pH 7.5], 150mM NaCl, 1 mM EDTA, 0.2% IGEPAL® CA-630, 0.5 mM DTT, 1X protease inhibitor cocktail [Roche]) in a 3:1 v/w ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in blocking solution containing 5% horse serum and 1% Western Blocking Reagent (Roche Diagnostics, Mannheim, Germany) in TNTx for at least 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2) supplemented with a cocktail of protease inhibitors (CLAPA and 1 µg/mL cOmplete, EDTA-free, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM pyridoxal 5’-phosphate hydrate (PLP) and proteases inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail Roche).
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bioRxiv - Immunology 2024Quote: ... Brain stems were cut into pieces and digested for 40 min at 37 °C with 5% CO2 in PBS supplemented with 1.6 IU ml-1 Collagenase/Dispase (Roche) and 0.5 mg ml-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer (80 mL per L of initial culture: 20 mM HEPES pH 8, 500 mM NaCl, 5% v/v glycerol, 0.5 mM TCEP + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
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bioRxiv - Cell Biology 2024Quote: ... and thawing and by resuspension in 5 mL of ice-cold Lysis/Wash buffer (25 mM HEPES pH 7.5, 1 M NaCl, 5% glycerol) supplemented with cOmplete protease inhibitor cocktail (Roche), followed by vigorous sonication on ice delivered by a Jencons Ultrasonic Processor (15s bursts at 70% amplitude) ...
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bioRxiv - Genetics 2024Quote: ... 1 mM DTT, 10 mM NaPPi, 5 mM EGTA, 5 mM EDTA, 0.1 mM Na3VO4, 5 mM NaF, and Roche cOmplete™ Protease Inhibitor Mix). Crude extracts were prepared by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) supplemented with one tablet ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche). The cell solution on ice was sonicated 3 × 1 min at 90% amplitude (0.5 s cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... and supplemented with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail tablet (Roche). Cells were lysed via high-pressure homogenization ...
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bioRxiv - Neuroscience 2021Quote: ... viral DNA was purified from SN (High Pure Viral Nucleic Acid Kit, Roche) and PCR was set up with Brilliant II qPCR Low ROX Master Mix (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from a 200µL sample using the MagnaPure24 (Roche) External Lysis Pathogen 200 protocol with elution into 50 µL ...
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bioRxiv - Microbiology 2022Quote: Viral DNA was extracted by the High Pure Viral Nucleic Acid Kit (Roche) and eluted in 40 μM (μL ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant removed and ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Roche, Lewes, UK) added to cell-free media ...
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bioRxiv - Pathology 2023Quote: ... 0.1% SDS and 0.5% deoxycholic acid) with cocktail protease and phosphatase inhibitors (Roche) at 4℃ condition ...
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bioRxiv - Cell Biology 2023Quote: ... Basal respiration was measured in DMEM containing 2% fatty-acid-free BSA (Roche). Cells were treated with 100 μM isoproterenol to stimulate respiration ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2% bovine serum albumin (BSA; fraction V, fatty-acid-free; Roche), 50 µM β-mercaptoethanol (Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol) supplemented with 5% v/v beta mercaptoethanol and 1x protease inhibitor cocktail (Roche) was added to each sample followed by centrifugation at 18,000×g at 4 °C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% Tween 20) for 5 min each and then incubated in the AP reaction buffer with NBT/BCIP substrate (Roche).
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation and the proteins in the supernatant were purified by gravity Ni-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Genomics 2021Quote: ... Cells from two biological replicates were lysed with ChIP lysis buffer (5 mM PIPES pH8, 85 mM KCl, 1% Igepal) with a protease inhibitor (PI) cocktail (Roche). Nuclei were collected by centrifugation at 2,000 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.
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bioRxiv - Microbiology 2020Quote: ... 30 mM imidazole, 1mM DTT, 0.75% CHAPS, 1 μM DNases, 5◻mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]). Purification was similar as the above instead of the elution buffer used (50 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...