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Citations for Roche :
8101 - 8150 of 8748 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Microbiology 2019Quote: ... 10 mM imidazole and 2 mM β-mercaptoethanol) supplemented with DNaseI (AppliChem) and Complete Protease inhibitor (Roche). After one passage through a French press cell ...
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bioRxiv - Developmental Biology 2019Quote: ... slides were washed in 0.2x SSC then transferred to MBST before blocking with 2% blocking solution (Roche) for at least 1 hr at RT ...
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bioRxiv - Cell Biology 2019Quote: ... samples were further incubated for 2 hrs in 40 µl pre-cleared protein-G agarose beads (Roche). Beads with immunocomplexes were centrifuged at 3,000 × g and washed four times in lysis buffer with intermittent incubations ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.05M Tris HCl pH 7.9, 5μM MgCl2, 5μM CaCl2, 2% SDS supplemented with 1x protease inhibitor cocktail, ROCHE) by sonication on ice ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM imidazole and 2 mM β-mercaptoethanol) supplemented with DNaseI (AppliChem) and Complete Protease inhibitor (Roche). After one passage through a French press cell ...
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bioRxiv - Microbiology 2020Quote: ... Cobas SARS-CoV-2 PCR Test for the Cobas 6800 System (Roche Molecular Systems, Branchburg, NJ, USA) was used to detect the presence of SARS-CoV-2 [11].
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bioRxiv - Genetics 2022Quote: ... The PCR procedure was performed by adding 25μL 2 × KAPA HiFi HotStart Ready Mix (KAPA biosystems, KM2602) with 2.5ul primers of NEB Oligos kit and 2.5ul Illumina universal primers ...
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bioRxiv - Genetics 2021Quote: ... Worms were then resuspended in 0.4 ml Buffer B70 supplemented with 2 × cOmplete Proteinase inhibitor cocktail (Roche) and dripped into liquid N2 with 1 ml pipette tips to form small pearls ...
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bioRxiv - Immunology 2020Quote: ... Fresh tissue was initially cut into small pieces and digested with collagenase VI (2 mg/ml; Roche) for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: Pellets of 1x107 cells were lysed with 1mL Co-IP Lysis Buffer (300mM NaCl, 50mM Tris HCL pH7.4, 0.5% NP40, 0.1% Sodium deoxycholate, 2% SDS) with PhosSTOP (Roche) and cOmplete (Roche ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Immunology 2020Quote: An aliquot was thawed and tested at NorthShore using the Elecsys Anti-SARS-CoV-2 (Roche Diagnostics), Access SARS-CoV-2 IgG (Beckman Coulter) ...
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bioRxiv - Immunology 2022Quote: ... Creatinine levels were measured with the COBAS INTEGRA Creatinine Jaffé Gen.2 Kit (Roche Diagnostics, Indianapolis, IN). To assess severity of proteinuria ...
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bioRxiv - Immunology 2022Quote: ... C57BL/6NCrl mice were given either 2% (w/v) Glucose water or 10% (v/v) Ethanol (Roche) and 2% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatants was aspirated and cells were lysed in 2 PCVs Buffer A containing 1x CPI (Roche, #11836145001), 0.5 mM DTT and 0.15 % NP40 ...
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bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
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bioRxiv - Physiology 2022Quote: Islets were isolated by injecting 2 mL collagenaseP (0.8 mg/mL in HBSS, Roche Diagnostics, Catalog # 11249002001) into the bile duct with the ampulla of Vater clamped ...
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bioRxiv - Microbiology 2022Quote: ... amplified using primers LN112 and LN113 (Table 2) and the PCR DIG Synthesis Kit (Roche, Basel, Switzerland) as a probe ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 7.5) and placed in RBR buffer (20% Goat serum, 2% Roche Blocking Reagent in 1x MAB) on the orbital rocker for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mM EDTA (pH 8)) supplemented with 2 mM Na3VO4 and complete protease inhibitor mixture (Roche). Lysates were first centrifuged at 4 °C for 15 min at 20.000 g and subsequently subjected to ultracentrifugation at 4° C for 30 min at 100.000 g ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl of target RNA were incubated for 15 min with 0.6 µg baker’s yeast tRNAPhe (Roche), 1 mM MgCl2 and increasing amounts of protein in a volume of 20 µl ...
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bioRxiv - Developmental Biology 2024Quote: Embryos were deyolked in cold-DPBS supplemented with cOmplete proteinase inhibitor cocktail (Roche, used at 2× concentration), extracted with RIPA buffer (50 mM Tris ...
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bioRxiv - Genomics 2024Quote: ... the embryos were transferred to pre-hybridization buffer (50% deionized formamide, 5x SSC pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Bioengineering 2024Quote: ... Pre-hybridization was carried out at 48°C for 2 hours in DIG Easy Hyb buffer (Roche). The trnI/A probe was denaturalized for 20 minutes at 68°C and used for hybridization overnight at 48°C ...
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bioRxiv - Cell Biology 2022Quote: ... Then samples were treated at 37 °C for 2 h with 500 µL of RNAse A (Roche) 2 mg/mL and after 30 min with 200 µL of pepsin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1, Roche) and protein G sepharose beads or ALFA selector ST beads (NanoTag Biotechnologies) ...
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bioRxiv - Cancer Biology 2023Quote: The beads were supplemented with a PCR mix comprising 20 μl of 2× HotStart Readymix (Kapa Biosystems), 4 μl of 5′ end biotin-modified P7 primer (10 μM ...
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bioRxiv - Immunology 2023Quote: ... after cutting SVF into small pieces and incubation in a 2 mg/ml collagenase D solution (Roche) at 37°C in a water bath for 45 min ...
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bioRxiv - Immunology 2024Quote: ... plus 2% fetal calf serum (Dutscher)) supplemented with 50μg/mL DNAse and 10μg/mL collagenase D (Roche).
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bioRxiv - Microbiology 2022Quote: ... At the end of the cell disruption dry homogenised powder of cells were resuspended in 1 ml urea buffer (8 M urea, 50 mM Tris/HCl pH 8.0 containing one tablet of ‘cOmplete’ protease inhibitor per 25 ml, Roche, Germany; 1 mM DTT; 0.1 M PMSF) and centrifuged at 16,000 × g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche, Sigma-Aldrich, St. Louis, MO, USA). Samples were multiplexed using Takara long amplicon Taq polymerase with GC buffers following the manufacturer’s instructions for PCR barcoding for nanopore sequencing (EXP-PBC096 Oxford Nanopore Technologies ...
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bioRxiv - Pathology 2021Quote: ... Tissue sections were then blocked with blocking solution buffer II (containing 0.1% Triton X-100 and 2% normal sheep serum) at room temperature for 30 min before covering with anti-DIG alkaline phosphatase conjugate anti-digoxigenin antibody (Roche, diluted 1:500 in buffer II) for 1 h at 45°C ...
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bioRxiv - Genetics 2019Quote: ... Skin fibroblasts from three controls and the affected individual were recovered in ice cold RIPA buffer with protease inhibitors (‘Complete EDTA-free’; Roche Diagnostics, 1 tablet in 10ml buffer) and 25mM N-ethylmaleimide (NEM ...
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bioRxiv - Immunology 2022Quote: ... All buffers were kept on ice and the final Cell Lysate Buffer was supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (#11836170001, Roche, 1 tablet per 25 ml) and PhosSTOP™ (#4906845001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Each cell pellet was resuspended in 35 mL of lysis buffer (50 mM Na-phosphate buffer pH 8.0, 500 mM NaCl, 14 mM β-mercaptoethanol, protease inhibitors [1 Roche Mini EDTA-free tablet/10 mL]). Cells were disrupted by sonication (4 times for 1 min with intermittent cooling on ice) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EGTA, 20 mM MgCl2, 500 mM NaCl, 0.5% NP-40, protease inhibitor cocktail [Roche], and PhosStop [Roche] containing 20 μg of GST-Rhotekin-RBD). The lysates were centrifuged at 20,000 ×g for 3 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were resuspended in an equal volume of 2x lysis buffer (2x TBS, 0.2% TX-100, and 10% glycerol with protease inhibitors (Roche, 1 tablet per 25 ml lysis buffer)) ...
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bioRxiv - Plant Biology 2024Quote: ... HA detection was performed with a directly coupled α-HA antibody (α-HA-HRP, high-affinity monoclonal rat antibody, 3F10, Roche, catalog no. 12013819001, 1:1000). Immunodetection was performed after three washes with TBST for 10 min each ...
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bioRxiv - Microbiology 2020Quote: ... Bound proteins were eluted with a 30 minute incubation at 4°C with Elution Buffer (50 mM Tris pH 8.0, 40 mM reduced glutathione, and Roche Complete protease inhibitors). At the end of the incubation ...
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bioRxiv - Cell Biology 2019Quote: ... cells were harvested through centrifugation at 5000 rpm for 10 min at 4°C and afterwards washed with 2mL PBS supplemented with 1x protease inhibitors cocktail (Roche, Basel, Switzerland). Cell pellet was resuspended in lysis buffer (150 mM KCl ...
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bioRxiv - Cancer Biology 2020Quote: ... 40 mM Tris-HCl pH 7.5, 20 mM MgCl2, and 4% Triton X-100 supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail), and 24 ml nuclease free water on ice for 20 min with frequent mixing ...
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bioRxiv - Microbiology 2021Quote: ... 500 mM NaCl and 4 mM β-mercaptoethanol (β-ME)) in the presence of an anti-protease cocktail (Complete EDTA free, Roche) and 1 ul benzonase (MERK Millipore 70746) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted at 10,000 RPM for 30 minutes at 4°C and resuspended in 40mL lysis buffer (20mM HEPES, pH 7.4, 1M NaCl, 1mM EDTA, 1mM DTT, Roche protease inhibitor cocktail). Lysozyme was added at a final concentration of 1mg/ml and cells were incubated on ice for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... To determine the dimerization of sFeoFL::GFP and sFeoN:GFP a Western Blot was generated from a 4-12% gradient SDS and a Native polyacrylamide gel side-by-side using a mouse anti-GFP antibody (Roche Nr. 11814460001) at 1:500 dilution ...
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bioRxiv - Genomics 2022Quote: ... then incubated with primary antibody at 4°C overnight in Wash Buffer (10 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) with 2mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... was first pooled before being divided into 700-µL aliquots in cryovials containing 28 µL of a 25x concentrated protease inhibitor cocktail solution (4% v/v) (cOmplete Protease inhibitor cocktail tablets, Roche, Mannheim, Germany) according to the manufacturers’ instructions and stored at -80 °C (Figure 10 – step III).
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bioRxiv - Neuroscience 2022Quote: ... The cell viability following 4-AP treatment was assayed by MTT assay according to manufacturer’s protocols (Roche, cell proliferation kit I (MTT), # 11465007001) ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked for 1 h (at room temperature) before incubating overnight at 4 °C in anti-dig sheep Fab fragments conjugated to alkaline phosphatase (AP; Roche No. 11093274910) diluted 1:2500 in blocking solution ...
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bioRxiv - Pathology 2020Quote: ... The specific pairs of primers used for the real-time PCR are listed in Supplementary Table 4 (designed by Universal ProbeLibrary Assay Design Center tool from Roche, Indianapolis, IN). The real-time PCR was performed with FastStart Universal Probe Master mix ...