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Citations for Roche :
8251 - 8300 of 8748 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and qPCR was carried out in 5 µL multiplexed reactions and 384-well format using the LightCycler 480 Instrument II (Roche). Final concentration of primers are 0.5 µM and probes are 0.25 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 µl of diluted cDNA was used for each qPCR reaction carried out using KAPA SYBR FAST master mix (Roche). Relative expression levels for each gene were determined based on the 2−ΔΔCT method normalizing against 7SK RNA.
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorophores with the least autofluorescence on FFPE tissue were selected to minimize false positives: Cyanine 5 (Cy5) (DISCOVERY CY5 Kit, Cat#760238, Roche Diagnostics Corporation ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Cell Biology 2024Quote: ... followed by an 8-minute perfusion with myocyte digestion buffer containing 5 mg of liberase dispase high DH enzyme (Roche) per digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was discarded, and 5 mL of Hank’s Balanced Salt Solution (Ca-, Mg-) (gibco) buffer containing liberase (Roche, #05401119001) (50 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Four hippocampi and 4 cortices from 8-month-old 5xFAD mice were homogenized in ice cold sucrose buffer (320 mM sucrose, 5 mM Hepes pH 7.4) supplemented with protease (Roche #05056489001) and phosphatase inhibitors (Invitrogen #A32957 ...
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bioRxiv - Immunology 2022Quote: ... frozen tissue fragments were thawed and incubated for 5 minutes at 37°C in a dissociation buffer containing RPMI-1640 supplemented with 10 U/mL DNase (Roche), 200 U/mL collagenase IV (Worthington Biochemical) ...
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bioRxiv - Cell Biology 2023Quote: ... Two brains were transferred to a pre-chilled 30-mL glass tube Wheaton homogenizer containing 5 mL of cold ‘solubilisation-plus’ buffer (solubilisation buffer with 1X COMPLETE protease inhibitor and 1X PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: For nuclear cell lysis 5×10^6 cells per condition were resuspended in 1mL of Lysis Buffer + protease inhibitor (cOmplete, Roche) (10mM Tris-HCl pH 7.4 ...
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bioRxiv - Genomics 2023Quote: ... Using a P7-specific primer (Extended Data Table S4) UMI’s were added to cDNA (in 5 reactions) with Kapa 2x HiFi HotStart ReadyMix (Kapa Biosystems). Reactions were pooled and purified ...
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bioRxiv - Biochemistry 2023Quote: ... and the pellet was resuspended in 5 volumes of the lysis buffer (buffer A including one EDTA-free protease inhibitor tablet (Roche); buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of co-IVT protein was added along with 5 μL (bed volume) of washed HA-beads (11815016001, Roche) in 300 μL binding buffer (25 mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of sample were used in a reaction mix containing 1 μL 10 μM of each primer (0.5 μM final) and 5 μL 2x Light Cycler SYBR Green LightCycler® 480 SYBR Green I Master (Roche). For telomere amplification ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5 % NP-40, 0.1 % Triton X-100, complete protease inhibitor cocktail [Roche] added fresh) for 10 min on ice ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were resuspended in binding buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 5 mM TCEP) supplemented with protease inhibitors (Roche cOmplete), sonicated ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Immunology 2023Quote: Whole lung single cell suspensions were prepared by harvesting lung lobes into 5 ml HBSS with 40µl Liberase Tm (0.1 U/ml, Roche, Cat# 5401127001) and 20µl DNAse 1 (10mg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed using the first-strand cDNAs diluted 5-fold in water and KAPA SYBR FAST qPCR Master Mix (2x) kit (Roche) and gene-specific primers in a LightCycler 96 (Roche).
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were serially applied using the U DISCOVERY 5-Plex IF procedure and the following reagents and kits (all from Ventana Medical Systems, Roche): DISCOVERY inhibitor (760-4840) ...
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bioRxiv - Genetics 2024Quote: ... Blots were then exposed to 2.5 pmol of 40-nt HPLC purified DNA oligonucleotides conjugated to digoxigenin (DIG) using the DIG Oligonucleotide Tailing Kit (Roche) hybridized to the nitrocellulose membrane at 60°C overnight (42°C for 2 h for 5S rRNA ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was washed in buffer A three times and then homogenized in 400 ul of buffer B (250 mM sucrose, 10 mM Tris HCl [pH 8.0], 10 mM MgCl2, 1 mM EGTA, 1X protease-inhibitor cocktail [11697498001, Roche], 0.1% Triton X-100, and 0.25% NP-40) for 10 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of DNA were used per reaction with the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of 2× SYBR Green Real-time PCR Master Mix (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Neuroscience 2019Quote: ... for 12 concentrations in sample buffer (0.1% BSA/0.05% Tween/PBS, 2 tablets of cOmplete Protease Inhibitor Cocktail (Roche Diagnostics Corporation ...
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bioRxiv - Genomics 2019Quote: ... Cell nuclei pellet was re-suspended in 2 mL FACS buffer (0.15 mM spermine, 0.5 mM spermidine, Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... rinsed in TBS/0.1% Tween-20 and incubated overnight in blocking buffer (TBS with 2% goat serum, 0.1% blocking reagent (Roche), 0.1% Tween-20 ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified carcinoma organoids were dissociated into single cell suspension in 0.05% trypsin with 0.1% EDTA supplemented with 2 U/ml of DNase I (Roche) for 2-3 min ...
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bioRxiv - Neuroscience 2020Quote: ... 40 mM HEPES, pH 7.4, 2 mM EDTA, 10 mM pyrophosphate, 10 mM glycerophosphate, 0.3% CHAPS, protease inhibitors from Roche) as indicated.
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bioRxiv - Molecular Biology 2020Quote: ... Patient cfDNA was isolated from 2 mL of plasma using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 genomic RNA was isolated from cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems, K4602) using specific primers for β-actin ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike protein monoplex IHC was conducted using a Chromomap DAB IHC kit (Roche, Basel, Switzerland) with CC1 antigen retrieval at 95°C for 64 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Afterwards samples were incubated for 2 h in the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) enzyme labeling solution at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... 3.4 mM KCl,10 mM CaCl2, 2.4 mM NaHcO3, 2 mM HEPES, pH7.0 and a protease inhibitor tablet [Roche]). The cell suspension was incubated at 4 °C for 15 mins with rigorous end-over-end rotation ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells were maintained in Myelocult medium supplemented with penicillin/streptomycin and 100 U/ml IL-2 (Roche). CRISPR-edited cell lines were generated by nu-cleofecting NK92 cells or CD34+ precursors with pCMV-Cas9-GFP all-in-one plasmid (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested for 30 minutes at 37 °C in DMEM + 2 mg/mL Collagenase A (Roche, Basel, Switzerland) + DNase (Roche ...
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bioRxiv - Immunology 2020Quote: ... patients with at least one nasopharyngeal swab positive for SARS-CoV-2 as determined by qRT-PCR (Roche LightCycler480 ...
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bioRxiv - Plant Biology 2019Quote: ... water was replaced with 100 μl of a 2 μg/ml horseradish peroxidase (type II; Roche, Rotkreuz, Switzerland) and 5 μM L-012 (WAKO Chemicals ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were resuspended in Lysis buffer (50 mM Tris pH 8.0, 300 mM NaCl, 2 mM DTT, 0.3 % TritonX-100, Roche Complete protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2021Quote: Lysis buffer 2x (200 mM Tris, 300 mM NaCl, 2% triton, pH 7.4) and Complete proteinase inhibitor (Roche) were added to homogenized organs for 30 min on ice ...