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Citations for Roche :
8001 - 8050 of 8748 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... 10% of cleared lysate was set aside to serve as input samples and the remainder was incubated at 4°C with anti-GFP antibodies (Roche #11814460001) for 3 h under gentle rotation ...
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bioRxiv - Neuroscience 2019Quote: ... mPFC tissue was incubated overnight at 4°C with an anti-DIG antibody coupled to horseradish peroxidase (Roche, Mississauga, ON, Canada) and mouse monoclonal anti-SMI-32 antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2020Quote: WB was performed on cell lysates obtained by harvesting cells with lysis buffer (Tris 125 mM pH 6.8, 4% sodium dodecyl sulfate, 20% glycerol) with Complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and sonicating ...
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bioRxiv - Genomics 2019Quote: We added UMIs to a total of 4 µg of DNA from each replicate split across eight reactions with KAPA2G Robust HotStart ReadyMix (Kapa Biosystems) for three cycles with a 65 °C annealing and 40 s extension ...
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bioRxiv - Microbiology 2019Quote: ... 4 μl of the cDNA sample together with 16 μl mastermix were combined and analyzed using a LightCycler Nano (Roche, Germany). The light cycler program was as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Cerebellar specimen gained from 4-weeks old Prpf8Y2334N mice were processed using KAPA RNA Hyperprep Kit with RiboErase (Kapa Biosystems/Roche) due to discontinuation of the Lexogen library construction kit ...
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bioRxiv - Neuroscience 2022Quote: ... Cerebellar specimen gained from 4-weeks old Prpf8Y2334N mice were processed using KAPA RNA Hyperprep Kit with RiboErase (Kapa Biosystems/Roche) due to discontinuation of the Lexogen library construction kit ...
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bioRxiv - Microbiology 2023Quote: ... All steps were performed at 4°C and buffers were all supplemented with protease inhibitor cocktail from Roche (cOmplete tablets, EASYpack). The pellets were washed once in buffer A (50 mM Tris/HCl pH8.0 ...
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bioRxiv - Immunology 2022Quote: Purified DNA from mouse fecal pellets or sorted cells was subjected to 16S variable region 4 PCR amplification using barcoded 515F and 806R primers (47) and the KAPA2G Robust HotStart ReadyMix (KAPA Biosystems), with the following cycling conditions ...
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bioRxiv - Cell Biology 2024Quote: ... assay was performed following our previous publication(4, 55) and the instruction of In Situ Cell Death Detection Kit TMR red (Roche, 12156792910). Cells were cultured on coverslips for 36 h ...
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bioRxiv - Neuroscience 2023Quote: ... Each brain with known weight was homogenized at 4°C using automated homogenizer and with IX RIPA buffer containing protease inhibitor tablet (Roche, Germany). After that the brain lysates were centrifuged at 12,000 rpm for 10 mins ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing (RNA-seq) libraries were prepared from 4 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche, KK8421) according to manufacturer’s specifications ...
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bioRxiv - Systems Biology 2024Quote: ... 1 ng was taken as a template for a PCR reaction (Supplementary Table 4, primers F: 42-65, R: 66-77) using KAPA HiFi HotStart ReadyMix (Roche, KK2602) for adding IIlumina indices and machine adapters ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were harvested using ice cold EBC buffer (140 mM NaCl, 50 mM Tris-HCl, 0.5% NP-40, 5 mM EDTA and protease inhibitor cocktail (Roche)) and briefly sonicated prior to 20 min centrifugation at 20,000 g ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM EDTA) with the addition of a Complete EDTA free protease inhibitor cocktail tablet (Roche Diagnostics, Oslo, Norway) and a PhosSTOP tablet (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... cells were collected and lysed with a lysing buffer (100mM KCl, 20mM HEPES, 5% Glycerol, 0.1X Triton-X, 1mM DTT, and Complete protease inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2019Quote: ... Resin was washed with TBS buffer (150 mM NaCl, 20 mM Tris-Cl, pH 7.4, 5 mM MgCl2, EDTA free protease inhibitors (Complete, Roche) and incubated with purified 6xHis-tag LC3 for 120 min @4°C in TBS buffer either alone or in the presence of purified 6xHis-Snapin ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The fixed embryos were resuspended in 2.5 ml of ice-cold lysis buffer (10 mM Tris-pH-8, 10 mM NaCl, 0.2% NP40 with Roche protease inhibitor cocktail freshly added ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM KCl, 5 mM MgCl2, 0.01% NP-40, freshly prepared 20 mM Na2MoO4, and cOmplete protease inhibitors; Roche). 10-20 million cells were lysed in 0.5 mL Felts buffer by rotation at 4°C for 30 minutes and vortexing for 10 seconds ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... which were blocked and permeabilized with 5% normal goat serum in 0.25% Triton X-100 in PBS (Roche Diagnostics) for 30 min at 25°C ...
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bioRxiv - Plant Biology 2021Quote: ... Each 10 μl reaction was comprised of 5 μl LightCycler 480 SYBR Green I Master mix (Roche Life Science), 2 μl cDNA template ...
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bioRxiv - Biochemistry 2021Quote: ... Pelleted cells were resuspended in 1x PBS buffer (pH 7.4) with 5 mM BME and proteinase inhibitor cocktail (Roche). For purification of PABPC1-His ...
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bioRxiv - Biochemistry 2020Quote: ... re-suspended with buffer A (50mM Phosphate buffer pH 8, 400mM NaCl, 5% glycerol, 1mM DTT, 0.5mM EDTA, protease inhibitor cocktail from Roche) and lysed using a microfluidizer followed by two cycles of centrifugation (12000 × g 20 min) ...
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bioRxiv - Microbiology 2020Quote: ... cells were centrifuged and pellets were resuspended in RB1 (20mM Tris pH7.5, 300mM NaCl, 1mM DTT, 5% glycerol and protease inhibitor (complete EDTA-Free, Roche)) ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
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bioRxiv - Plant Biology 2021Quote: ... 500 nM of each primers and 5 μl of 2X LightCycler® 480 SYBR Green I Master mix (Roche) in 10 μl final volume ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 5 times and subjected to real-time PCR using LightCycler 480 SYBR Green I Master (Roche) with specific primers for the genes of interest ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells from mouse blood infected with the DEH-GFP-expressing parasite were pelleted and then lysed in hypotonic buffer (10 mM Tris-HCl pH 8.4, 5 mM EDTA) containing protease inhibitors (Roche), freeze/thawed twice ...
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bioRxiv - Biophysics 2021Quote: ... The troponin T detection kit (Elecsys® Troponin T Gen 5 STAT) and troponin T were purchased from Roche. 1X phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... were harvested in ice-cold lysis buffer (0,25% NP40, 5% glycerol, 50 mM Tris HCl, 150 mM NaCl) containing protease inhibitor (Roche), DNAse (5 µl/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed once with ice-cold 1X PBS and then swelling buffer (5 mM HEPES pH 8, 85 mM KCl, 0.5% IGEPAL-CA630, protease inhibitor cocktail (Roche)) was added to the cells ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES, pH 7.5, 300 mM NaCl, 5% glycerol, 30 mM imidazole, Complete EDTA-free protease inhibitor [Roche]) and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... in lysis buffer (50 mM Tris–HCl pH 7.5, 300 mM NaCl, 10 mM imidazole, 5 mM β-mercaptoethanol, 0.5% Triton X-100, Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... re-suspended with buffer A (50mM Phosphate buffer pH 8, 400mM NaCl, 5% glycerol, 1mM DTT, 0.5mM EDTA, protease inhibitor cocktail from Roche) and lysed using a microfluidizer followed by two cycles of centrifugation (12000 x g 20 min) ...
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bioRxiv - Biochemistry 2022Quote: ... PSH in DDM micelles or in POPC vesicles was diluted with MES-buffer (50 mM MES pH 6.0, 500 mM NaCl, 5 mM MgCl2, 1x PI mix complete (Roche)) to 1 - 2 µM PSH ...
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bioRxiv - Biochemistry 2022Quote: ... pelleted cells were resuspended in lysis buffer (20 mM Tris-HCl pH 7.5, 500 mM NaCl, 5 mM MgCl2 with EDTA-free protease inhibitor (Roche)) ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) and incubated on ice for 10’ to isolate the cell nuclei ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Microbiology 2020Quote: ... Mycelia pellets were thawed on ice and resuspended in 0.4 mL ice-cold lysis buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Roche]) and sonicated (5x 15 sec on/15 sec off at 5-micron amplitude) ...
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bioRxiv - Molecular Biology 2019Quote: ... The E.coli cells were resuspended in lysis buffer (20mM HEPES, pH 7.5, 500mM NaCl, 5% glycerol supplemented with 1X PIC (Roche), 100ug/ml lysozyme and lysed by sonication and centrifuged ...
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bioRxiv - Microbiology 2019Quote: ... Transfected cells were washed two times in cold PBS and lysed in lysis buffer (20 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 0,2 % Triton X100, 10 % Glycerol, protease inhibitors (Roche)) ...
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bioRxiv - Molecular Biology 2019Quote: ... up to 5 ng/sample of ChIP was prepared for sequencing using the Kapa Hyper-prep Kit (Kapa Biosystems) using NEXTflex adapters (Bio Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... frozen HEK293E cells were thawed and resuspended in 2x excess of lysis buffer (25 mM HEPES-KOH pH 7.6, 5 mM MgCl2, 50 mM KCl, cOmplete protease inhibitor cocktail (Roche), 1 mM PMSF ...
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bioRxiv - Genetics 2020Quote: ... 721-bp tandem and 1600-bp tandem repeats were selected and PCR labelled with tetramethyl-rhodamin-5-dUTP (Roche) (Ishii et al ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in ice-cold lysis buffer (10 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, supplemented with a protease cocktail inhibitor from Roche, Switzerland) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2019Quote: ... cortices from 6-week old mice were homogenized in cold sucrose buffer (20 mM HEPES pH 7.4, 320 mM sucrose, 5 mM EDTA) with protease inhibitor cocktail (Roche). Homogenates were centrifuged at 3,000 g for 20 min at 4°C speed to pellet nuclei ...
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bioRxiv - Physiology 2021Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...