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Citations for Roche :
751 - 800 of 1261 citations for IL 3 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The DNA probes were 3′ end-labelled using digoxigenin (DIG)-11-ddUTP and terminal transferase (Roche, Basel, Switzerland). Binding of TFRs to DNA was analyzed using the DIG Gel Shift Kit ...
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bioRxiv - Microbiology 2022Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 x g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Colorimetric signals were generated using a development solution containing 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were incubated at 37°C for 3-4 h before adding DNase I (0.1 U/µl) (Roche) and incubating for another 30 min at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... planulae were washed twice with 1/3 strength artificial seawater and incubated with 50 μg/mL liberaseTM (Roche) at 37 °C for 10–20 min with occasional pipetting ...
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bioRxiv - Immunology 2023Quote: ... and single-cell suspensions of lung mononuclear cells were prepared by Liberase Blendzyme 3 (70 ug/ml, Roche) digestion containing DNaseI (30 µg/ml ...
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bioRxiv - Biochemistry 2024Quote: Cell metabolic viability was measured by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Roche). After treatments ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was diluted 1:3 and qRT-PCR was conducted using a SYBR Green master mix (Roche, FSUSGMMRO) on a Light Cycler 480 (Roche) ...
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bioRxiv - Physiology 2021Quote: ... containing 25 ng/mL mouse anti-HA antibody conjugated with peroxidase (Roche, 12013819001) and 4% non-fat skim milk ...
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bioRxiv - Genetics 2021Quote: ... Two corneas (per mouse) were incubated in 15 mg/ml Dispase (4942078001, Roche) in DMEM (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used in this study are as follows: mouse anti-GFP (Roche) and mouse anti-Actin (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) was used in the immunostaining assay.
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bioRxiv - Neuroscience 2021Quote: ... or anti-GFP mouse monoclonal antibody (1:200, 11814460001, clones 7.1, 13.1, Roche), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... 4A the following antibodies were used: mouse anti-HA 12CA5 1:1000 (Roche), rat anti-HMR 2C10 1:25 (Helmholtz Zentrum Munich) ...
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bioRxiv - Synthetic Biology 2021Quote: The primary antibodies used were mouse anti-GFP (clones 7.1 and 13.1) (Roche) at a dilution 1:5000 for SGR57 and SGR58 ...
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bioRxiv - Plant Biology 2020Quote: ... Primary antibodies were α-GFP (mouse) and α -HA (rat; both from Roche), α –EDS1 (Rabbit ...
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bioRxiv - Plant Biology 2021Quote: ... that had been pre-conjugated with a monoclonal mouse anti-GFP antibody (Roche) at room temperature for 2 h ...
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bioRxiv - Genomics 2022Quote: ... incubated with anti-DIG1 (mouse anti-digoxigenin, 1:100, Roche Diagnostics, Basel, Switzerland) and streptavidin Cy3 conjugate (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblotting followed established protocols using mouse anti-HA monoclonal antibody (1:1,000) (Roche). Detection was done using the Odyssey Clx LICOR system using goat anti-mouse IRDye800WC (1:10,000) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected with primary antibodies mouse mAb α-GFP (Roche Diagnostics #11814460001), 1:1000 and rabbit α-PfHP1 12 ...
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bioRxiv - Cell Biology 2020Quote: ... The oocytes were immunostained with anti-GFP mouse antibody (1:200 dilution; Roche) followed by anti-mouse IgG-Alexa Fluor 488 antibody (1:200 dilution ...
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bioRxiv - Plant Biology 2019Quote: ... Western blotting was carried out using mouse anti-GFP 11814460001 (Roche, Basel, Switzerland) and rabbit anti-Histone H3 AS10710 (Agrisera ...
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bioRxiv - Cell Biology 2019Quote: GFP-Pav and Tum proteins were incubated with mouse anti-GFP antibody (Roche) overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: ... Primary antisera and dilutions were: mouse anti-HA monoclonal (clone 12CA5, Roche; 11583816001), 1/10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-HA was from Covance (Suffolk, UK; mouse monoclonal MMS101P) or from Roche (Wellwyn Garden City ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used in Western blot analysis: monoclonal α-GFP (1:1000; mouse; Roche), α-GAPDH (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... For3-GFP fusions were detected with a mouse monoclonal anti-GFP antibody (Roche), with anti-cdc2 (PSTAIR ...
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bioRxiv - Microbiology 2022Quote: ... and mouse anti-GFP in a dilution of 1:1000 (α-GFP, ROCHE). For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... HA (12CA5, Mouse mAb, cat# 11583816001, Roche, at 1 μg/mL final concentration); MTA2 (Rabbit polyclonal antibody ...
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bioRxiv - Cell Biology 2023Quote: Antibodies were applied in the following dilutions: mouse a-GFP (1:1000) (Roche), rat a-HA (1:2000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and then exposed to mouse anti-digoxigenin antibody (Roche, #11333062910; 1/100 dilution) or rabbit anti-fluorescein antibody (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Molecular Biology 2023Quote: Mouse aortic arches were incubated in digestion buffer containing liberase (Cat. # 273582, Roche), hyaluronidase (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used in this study were mouse anti-BrdU antibody (11170376001, Roche), goat anti-GFP (ab6658 ...
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bioRxiv - Developmental Biology 2023Quote: ... All genotyping reactions were performed with KAPA HotStart Mouse Genotyping Kit (Roche, 07961316001) with 1 μL of gDNA and a final primer concentration of 5 μM each ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of the culture was then treated with either 5 µl of MeOH or LMB dissolved in 7:3 MeOH:H2O solution (Roche) at a final concentration of 50 ng/mL for 45 min before imaging.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice with 0.1% Triton-X in PBS and blocked with 3% BSA (constituted from powder BSA, Roche Fraction V ...
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bioRxiv - Molecular Biology 2019Quote: ... 5–10 × 106 HEK-293 or MEF cells were washed with cold sucrose-containing solution I (0.5 M sucrose, 3 mM MgCl2 with Cocktail protease inhibitor, Roche), harvested by scraping into 1 ml solution I and sonicated in a BioRuptor for 10 cycles (15 sec ON/15 sec OFF) ...
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bioRxiv - Cell Biology 2019Quote: ... ∼100 µl glass beads and 100 µl of lysis buffer (50 mM Tris-HCl pH 8.0, 1 mM EDTA, 15 mM Tris pH 9.5, 3 mM DTT, 1X cOmplete EDTA-free inhibitor cocktail [Roche]) were added to each dried pellet ...
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bioRxiv - Neuroscience 2019Quote: ... The plates were then washed 3 times with ~300 μl of wash buffer (0.05%Tween in PBS) and blocked with 150 μl of 3% BSA (Fraction V, protease-free, Roche Diagnostics Corporation ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Immunology 2021Quote: ... The pancreas was inflated via the common bile duct by adding ∼3 ml of 0.8mg/ml Collagenase P (Roche) and 10µg/ml Dnase I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed 3 times for 5 min each in PBST and blocked with 1X Blocking Reagent (Roche) in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... 12-20 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) with 2.3 U of Liberase Blendzyme 3 recombinant collagenase (Roche) were cannulated into the vena cava to isolate hepatocytes ...
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bioRxiv - Microbiology 2021Quote: ... 200 μg each lysate was pre-cleared with 20 μl of Protein A agarose beads (3 mg/ml, Roche) in 200 μl for 1 hour at 4°C on a Labquake Rotator (Barnstead Thermolyne) ...
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bioRxiv - Cell Biology 2020Quote: RACE-cDNA was synthesized according to the manufacturer’s protocol for 5’/3’ RACE Kit 2nd generation (Roche; Cat.No. 03353621001). The 2648-bp cDNA was cloned into the NheI-XhoI site in pcDNA 3.1 plasmid to obtain pcDNA3.1-DR5-AS and was verified by sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Neuroscience 2022Quote: ... and then reacted with 0.375 mg/mL nitroblue tetrazolium and 0.188 mg/mL 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP; Roche Diagnostics) for 27—42 h.
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bioRxiv - Physiology 2022Quote: ... for 3 min and incubated twice for 3 min with CSK buffer containing 0.5% Triton X-100 and complete protease inhibitors cocktail (catalog no. 11873580001, Roche). Cells were after washed with PBS-S ...
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bioRxiv - Developmental Biology 2020Quote: ... Sorted cells were pelleted for 5 min with 2000 rpm at 4°C and resuspended in 500 ul hypotonic buffer (HB; 20mM Tris-HCl, pH7.5, 10mM NaCl, 3 mM MgCl2) with added protease inhibitor (Roche). After 30min incubation on ice ...