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Citations for Roche :
7651 - 7700 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... was diluted 10-fold with ChIP incubation buffer (70 mM NaCl, 10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 2 mM EDTA, 0.1% Triton, 1 x Roche Complete EDTA-free Protease inhibitor cocktail) ...
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bioRxiv - Immunology 2024Quote: ... followed by a subsequent incubation at 95 °C in pre-formulated Cell Conditioning 1 solution (Roche Diagnostics, Canada). Following this ...
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bioRxiv - Plant Biology 2024Quote: ... supplemented with 1 tablet of protease inhibitor cocktail per 10 ml buffer (cOmplete™ Proteasehemmer-Cocktail, ©Roche) with an ultrasonic homogenizer (Hielscher Ultrasonics UP200St ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were stopped by adding 0.5 μl ethylenediaminetetraacetic (0.5 M EDTA) and 1 μl Proteinase K (19 mg/ml, Roche), and incubated at 50°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and 72°C for 1 min) All PCR was performed using KAPA HiFi HotStart ReadyMix (Kapa Biosystems; KK2602). The PCR products were isolated by QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 mM NaCl, 4 mM MgCl2, 2 mM DTT, 1% SDS, 0.5% sodium deoxycholate, 0.5% Igepal, supplemented with Roche Complete Protease Inhibitor cocktail (1 tablet/50 mL)) ...
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bioRxiv - Microbiology 2024Quote: ... and its activity was measured by addition of 1 mM chlorophenol red-β-D-galactopyranoside (Roche Diagnostics, GmbH) in 100 mM HEPES pH 8 (Dutscher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Pull-down was performed on the cell lysates by adding 1 µg of α-HA (Sigma Roche 12CA5) or mouse α-IgG (Santa Cruz Biotechnology sc-2025 ...
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bioRxiv - Cell Biology 2024Quote: ... Bound protein was eluted by heating immunoglobulin G beads with 35 μl of denaturing urea SDS-loading dye (1% SDS, 8 M urea, 10 mM Tris, pH 6.8, 10 mM EDTA, 0.01% bromophenol blue, 1x Roche Complete inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... 300 mM NaCl, 3.0 mM MgCl2, 2 mM DTT, 0.2 % Triton X-100, 1 tablet cOmplete Mini, Roche) and 150 μL MQ ...
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bioRxiv - Molecular Biology 2024Quote: ... NaCl 140 mM, EDTA 1 mM, Glycérol 10 %, NP40 0.5 %, AEBSF 1mM, Protease inhibitor cocktail Complete 1x, Roche) and resuspended in 30 µl of Laemmli buffer (1 x) ...
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bioRxiv - Systems Biology 2024Quote: ... 1 mL of lysis buffer (25 mM Ammonia Bicarbonate (AmBic) with protease inhibitors (Roche complete protease inhibitor tablets)) was used to resuspend the thawed pellets and the sample was transferred to a 2 mL screw cap vial for bead beading in 2 mL Precellys vials prefilled with 0.5mm glass beads ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was performed on LightCycler© 480 thermocycler using the SYBR Green 1 Master kit (Roche, Switzerland). Primers were used at 200 nM ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) supplemented with complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche) and 1 mg/ml lysozyme (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... harvested and lysed 14 h later in 300 µl binding buffer (20 mM HEPES pH 7.6, 150 mM MgCl2, 10% glycerol, 0.05% NP-40, 1 mM DTT, ROCHE cOmpleteTM ULTRA-tablet Mini protease inhibitor) ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in 150 µl of lysis buffer (PBS 1X, 1% protease inhibitor cocktail EDTA-free, Roche) and lysed by sonication (amplitude 70% ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were then probed with either Horse Radish Peroxidase (HRP)-conjugated 3F10 anti-HA (1:2000, Roche), HRP-conjugated FLAG M2 (1:5000 ...
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bioRxiv - Pathology 2024Quote: ... Slides were incubated for 30 minutes with 1 unit of DNase I (Roche, Cas. No. 9003-98-9) at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.25% CHAPS, 5 mM ATP, 5 mM MgCl2, 0.25 mM EDTA, 2 mM DTT, 10% glycerol, 1× Roche cOmplete protease inhibitors ...
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bioRxiv - Microbiology 2024Quote: ... Nuclei were centrifuged at 4°C at 1,300 x g for 4 min and then resuspended in 1 mL of Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, supplemented with Roche complete EDTA-free protease inhibitor tablet) ...
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bioRxiv - Pathology 2024Quote: ... by directly assessing 1 drop of whole blood using an Accu Chek Aviva glucose meter (Roche Diagnostics, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Crosslinked tissue was resuspended in 1.5 mL lysis buffer (1X PBS, 1% NP-40, 0.5% NaDOC and 0.1% SDS with cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% (v/v) Triton X-100) to leave only matrix before treatment with 10μg/ml DNAse 1 (Roche) to cleave phosphodiester linkages in the DNA backbone ...
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bioRxiv - Immunology 2024Quote: ... were isolated by incubating specific nitrocellulose membrane fragments with PK buffer (100 mM This-Cl pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.2 % SDS and 20 U of proteinase K [Roche]). After 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The beads-bound nuclei were resuspended in 200 µl of Tween-Wash buffer (0.1% Tween-20, 20 mM HEPES-potassium hydroxide, pH 7.5, 150 mM sodium chloride, 0.5 mM spermidine, 1 Roche cOmplete EDTA-free tablet per 50 ml buffer and 0.1% BSA ...
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bioRxiv - Genetics 2024Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Genetics 2024Quote: ... resuspended in 900 μl shearing buffer (0.1% SDS, 10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with protease inhibitor (Roche), split into two~500 μl aliquots and sonicated to fragment sizes ranging 500–1000 bp with a Covaris system (E220 Focused-ultrasonicator ...
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bioRxiv - Genomics 2024Quote: ... and 1 μl of 10 mM of each primer using LightCycler® 96 System (Roche Diagnostics, Mannheim, Germany). The thermal cycler was programmed as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μl of forward and reverse primers (each 10 μM) and 10 μl of SYBR Green I (Roche) were combined to a total volume of 20 μl ...
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bioRxiv - Cell Biology 2024Quote: ... 10 mM KCl, 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM dithiothreitol [DTT], a protease inhibitor cocktail [Roche]). Triton X-100 (0.1% ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended for lysis in RIPA buffer (50 mM Tris pH 7.6, 150mM NaCl, 1% Triton X-100, 0.1% SDS) with protease (04693159001, Roche) and phosphatase inhibitors (524625 ...
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bioRxiv - Developmental Biology 2024Quote: ... were added to freshly prepared DB1 buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5 mM spermidine, 2% glycerol, 1× EDTA-free with Roche complete protease inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... Cartilage was then digested for 30 minutes at 37°C in 1 mg/ml pronase (Roche, Basel, Switzerland) and then overnight at 37°C in 1 mg/ml collagenase P (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellets were then resuspended in 400 uL Lysis buffer (1% SDS, 50 mM Tris Hcl pH 8.0 20 mM EDTA, protease inhibitors (Roche) and incubated on ice for 10 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... in liquid nitrogen in a 1.5-ml Eppendorf tube and resuspended in ice-cold lysis buffer (50 mM Tris pH 7.6, 10 mM EDTA, 150 mM NaCl, 1% Triton, supplemented with complete protease inhibitor, Roche Ref ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was lysed with 20 ml of Eps8L2 Lysis Buffer (10 mM Tris.HCLpH8, 150 mM NaCl, 1 mMEDTA, lysozyme 0.1 mg/ml, Pefabloc® (Roche) 0.5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in lysis buffer supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... pH 8.0, 100 mM NaCl, 5mM MgCl2, 1% Triton X-100, 1mM PMSF, and complete protease inhibitor cocktail; Roche) and lysed twice with high pressure (1200 psi ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Immunoprecipitation buffer (150 mM NaCl, 10 mM Hepes, 2 mM EDTA, 1% Triton, 1.5 mM MgCl2, 10% glycerol, protease inhibitor from Roche) and centrifuged at 14,000 × g ...
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bioRxiv - Cell Biology 2020Quote: ... Magnetic beads were washed 2 times with lysis buffer and 4 times with washing buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mg/ml Pefabloc SC (Roche), and EDTA-free protease inhibitor cocktail (cOmplete Tablets ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 mL ddH2O and resuspended in 100 μL lysis buffer (50 mM Tris, pH 7.4, 50 mM dithiotreitol, 1 mM EDTA, cOmplete EDTA-free protease inhibitor cocktail (Roche) and Phosstop (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... Wing discs of L3 larvae were hybridized with probes overnight at 56 °C using standard procedures and visualized using anti-Dig-AP (1:1,000; Roche). Primers used for generating PCR templates are listed in Key Resources Table.
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bioRxiv - Developmental Biology 2021Quote: ... 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide, 1 mg/ml tRNA from Roche, 5 mM EDTA from Sigma and 50 μg/ml Heparin from Sigma ...
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bioRxiv - Biophysics 2021Quote: ... The cell pellet was resuspended in 1 ml of polysome lysis buffer supplemented with EDTA-free protease inhibitors (Roche) and was grounded in liquid nitrogen using an RNase-free mortar and pestle ...
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bioRxiv - Developmental Biology 2021Quote: ... Eye discs were then removed from the mouth hooks and blocked for 1 hour in 1X PBX:Western Blocking Reagent (Roche) at room temperature with nutation ...
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bioRxiv - Developmental Biology 2021Quote: ... apob-1 and apob-2 levels were detected using the Fast Start Essential Green DNA master mix (Roche 06924204001) on a Roche LightCycler 96 instrument ...
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bioRxiv - Immunology 2021Quote: ... were lysed using for one hour with ice-cold 1% NP-40 lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA) supplemented with complete mini (Roche) and HALT (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... they were hybridized overnight at 65°C for three nights and incubated overnight with 1/2,000 alkaline-phosphatase conjugated with anti-DIG Fab fragment (Roche) at 4°C for three nights ...
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bioRxiv - Developmental Biology 2020Quote: ... Column was washed three times with wash buffer (PBS 2X, Triton X-100 0.1%, imidazole 50 mM, NaCl 500 mM, DTT 1 mM, 1x Complete protease inhibitor [Roche]) before storage in 50% glycerol (0.01 % Triton) ...
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bioRxiv - Developmental Biology 2021Quote: ... treatment for 1 hour at 37°C and cDNAs were amplified with KAPA HiFi Hotstart Ready Mix (Roche, KK2602). PCR reactions were conducted as follow ...