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Citations for Roche :
7501 - 7550 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and 0.1 mM CaCl2) with or without DNase I (final concentration 160 U mL-1, Roche, cat. # 04716728001) and were incubated at 37°C for 3 h ...
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bioRxiv - Cancer Biology 2022Quote: ... For enteric glial cell experiments were performed by hypotonic lysis in 200 µl of buffer A (20 mM HEPES, pH 8.0, 1 mM DTT, 0.1% NP-40 and a proteinase inhibitor cocktail from Roche). The cell lysates were incubated on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Fab fragments diluted 1:10,000 in the blocking buffer for 30 minutes at room temperature (Roche, Cat#11093274910) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... were incubated at 4°C with hypotonic lysis buffer (10 mM Tris–HCl pH 8.0, 1 mM KCl, 1.5 mM MgCl2, with protease inhibitors (ROCHE)) for 2 hours while rotating ...
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bioRxiv - Plant Biology 2022Quote: ... Cell pellets were thawed on ice and resuspended in lysis buffer (20 mM sodium phosphate pH 6.8, 10 mM DTT, 1 mM EDTA, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice and centrifuged for 10 min at 500 g and 4 °C to remove intact cells and cell debris ...
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bioRxiv - Epidemiology 2020Quote: ... Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science, Germany), using 200 nM of primers and probes in a final volume of 12 µl and 2 µl of control DNA were added ...
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bioRxiv - Molecular Biology 2020Quote: ... and finally for 5 min in a 1:100 dilution of the substrate CSPD or CDP Star (Roche) in buffer 3.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 1ml of cold Lysis Buffer 1 (10mM Tris pH 8.0, 10mM NaCl, 0.2% IGE-PAL, Protease inhibitor (Roche)) and incubated on ice for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Biochemistry 2020Quote: ... PFN1-KO or PFN2-KO) were split 1:3 and the next day transfected using XtremeGene 9 (Roche) with 5 μg V5-NAA80 (M23L ...
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bioRxiv - Physiology 2020Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF)) supplemented with EDTA-free protease inhibitor cocktail and phosphatase inhibitors cocktail (Roche). Tissue lysates were centrifuged at 14,000 rpm for 20 min at 4°C twice before using the supernatant for western blot studies ...
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bioRxiv - Microbiology 2021Quote: ... Plasma viral load in ART-treated PLWH was measured using the Amplicor HIV-1 monitor ultrasensitive method (Roche).
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mM MgCl2) supplemented with 0.5% Triton X-100 and 1 pill Complete® protease inhibitor cocktail (Roche). The cell lysates were subjected to two 5 sec pulses of sonication at the lowest settings (Branson Sonifier 250 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1 × protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 μl/10 mg) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells are pelleted and resuspended in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1X Roche complete inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2021Quote: Lysates for Western-blot were made in a 1% triton buffer including cOmplete protease inhibitor cocktail (Roche 11697498001) and Western-Blot and flow cytometric staining was performed as described previously (41),(42) ...
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bioRxiv - Microbiology 2020Quote: ... 0.05 μL of 20 μg/μL (1 μg BSA/reaction) of nonacetylated bovine serum albumin (BSA, 10711454001, Roche), 1 μL SuperScript™ III RT/Platinum Taq Mix and 1.85 μL of primer/probe mix (2019-nCoV CDC EUA Kit ...
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bioRxiv - Microbiology 2021Quote: ... A 1 uL aliquot was used to amplify the V1-V3 region using HiFi HotStart ReadyMix (KAPA BioSystems) and the Illumina PCR protocol ...
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was assessed by WST-1 assay as per manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies ...
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bioRxiv - Genomics 2021Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Genetics 2020Quote: ... 200 μl lysis buffer (8M Urea, 1% SDS, 50 mM Tris pH 8.5, Roche protease and phosphatase inhibitor) were added to the pellet and samples were vortexed and sonicated on ice ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM benzamidine) supplemented with 0.1 mg/mL lysozyme and one Complete EDTA-free protease inhibitor tablet (Roche) per 100 mL ...
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bioRxiv - Genomics 2021Quote: ... 250 nL of protease mix was added (1× NEB CutSmart buffer, 1.0 mg/mL Proteinase K (Roche, 000000003115836001)) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 mM imidazole) and incubated after addition of 1 or 10 mM phenylmethanesulfonyl fluoride (PMSF) (Roche, Basel, Switzerland), or 1 mM H2O2 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation in LNCaP IL1B overexpression and vector cells was assessed using Cell Proliferation Reagent WST-1 (Roche), per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Biophysics 2021Quote: ... Isolated membrane fractions were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.0, 20 mM KCl, 1 mM MgCl, 2 mM EDTA, pH 7.5 and protease inhibitor cocktail, Roche) from cells grown in EMM with appropriate glucose concentrations and with or without thiamine (5μg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was carried out using LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany) following the manufacturer’s temperature program with 62°C as the primer hybridization and elongation temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in lysis buffer (1.5M NaCl, 20 mM Phosphate buffer pH 7.5, 20 mM Imidazole, 10mg/mL lysozyme, 1 tablet of Roche EDTA-free protease inhibitor cocktail Millipore Sigma 11873580001 ...
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bioRxiv - Immunology 2021Quote: ... minced thymuses from fetuses and postnatal mice were digested with 0.5 and 1 unit/ml Liberase TM (Roche) in the presence of 0.01% DNase I (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... wild-type or transgenic animals in an equal volume of Pedro’s buffer (30mM HEPES, pH 7.5, 100mM KOac, 10mM EDTA, 10% glycerol, 1 mM DTT, 1x Roche complete mini protease inhibitors ...
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bioRxiv - Immunology 2021Quote: ... and digested for 20 min at 37 °C in medium supplemented with 1 mg/mL Collagenase D (Roche) and 2000 U/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10000; Roche Diagnostics, Germany) conjugated with alkaline phosphatase ...
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bioRxiv - Microbiology 2022Quote: ... Uninfected erythrocytes were removed using saponin buffer (0,1% saponin/PBS plus 1× cOmplete™ protease inhibitor cocktail (Roche)) ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG was detected with anti-DIG Fab fragments conjugated to alkaline phosphatase (Roche, Indianapolis IN; 1:2000 dilution). Alkaline phosphatase activity was revealed using NBT/BCIP (Roche ...
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bioRxiv - Immunology 2022Quote: ... The pellets were further digested in the presence of 1 mg/ml of collagenase/dispase (Roche Applied Science) and 1 mg/mL DNase in DMEM for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA (1 μg per replicate) was used for library preparations using the KAPA RiboErase Kit (HMR) (Roche) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated with immature and mature DC lysates prepared in RIPA buffer (10 mM HEPES, 50 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, protease inhibitor cocktail (Roche), 1 mM Na3VO4,1 mM DTT ...
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bioRxiv - Developmental Biology 2022Quote: ... The bound ribopropbe was detected using an alkaline phosphatase-conjugated goat anti-DIG Fab fragment (1:750; Cat#: 11093274910, RRID:AB_514497; Roche) and the HNPP/FastRed detection kit (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were re-fixed in 4% PFA for 5 min and incubated in 0.2 M HCl for 10 min at RT before treatment with tri-ethanolamine buffer (662.5 µl tri-ethanolamine, 112 µl 1 M HCl, 49.1 µl H2ODT, 125 µl acetic anhydride; Roche). Slides were preincubated at 65°C in hybridization buffer (50% formamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail and PhoshoStop phosphatase inhibitor tabs (Roche) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Cells or tissue powders were directly dissolved in 1 ml of the TriPure Isolation Reagent (Cat. 11667165001, Roche) and extracted using a column-based kit (Cat ...
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bioRxiv - Genomics 2022Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Genomics 2022Quote: ... Two rounds of washes with Wash Buffer were then performed and nuclei were incubated with ME-A loaded pA-Tn5 diluted 1:200 in 300 Wash Buffer (20 mM HEPES pH7.5, 300 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail ...