Labshake search
Citations for Roche :
6951 - 7000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Each TaqMan qPCR reaction consisted of 1x LightCycler® 480 Probes Master (Roche, Cat# 04707494001), 1x Primer Probe Master (predesigned assays for IDUA ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mini-tablet of EDTA-free protease inhibitor (Roche #04693159001)) ...
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bioRxiv - Molecular Biology 2023Quote: We designed a multiplex quantitative PCR (qPCR) with four parallel assays to be run on the Roche 480 light cycler (Roche, Basel, Switzerland). Compatible fluorescent dyes (FAM ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with 10 ml of cold 1x PBS supplemented with proteinase inhibitors and phosphatase inhibitors (Roche # 4693132001) and the pellets were snap frozen in liquid nitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... using FastStart Universal SYBR Green Master Mix (Rox) (Roche Life Science). Relative expression was determined by the 2-ΔΔCt method (130 ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were processed for proteolytic digestion with proteinase K (Roche) and incubated with TUNEL solution ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-HA-peroxidase (Roche, Sigma-Aldrich, 1/1,000), anti-Actin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Metagenomic DNA libraries were prepared using KAPA Hyper P Kits (Roche, Switzerland), and each sample was sequenced in 3 different lanes (3 technical replicates per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... For detection of in situ cell death (Roche), sections were processed for proteolytic digestion with proteinase K (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... rat monoclonal anti-HA-Peroxidase high affinity (Roche, Sigma-Aldrich, 12013819001), rabbit polyclonal anti-Actin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP (Roche 11 814 460 001, 1:500), TRF1 (Rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% blocking reagent (Roche). The first probe (0.8μM ...
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bioRxiv - Molecular Biology 2023Quote: ... and KAPA HiFi Uracil+ mastermix (Roche, KK2801/2) using the following protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 1x EDTA-free protease inhibitor cocktail (Roche), and 50 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100) with cOmplete EDTA-free Protease Inhibitor cocktail (Roche) and lysed by rotating for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: Cells were lysed with RIPA buffer supplemented with protease inhibitor cocktail (Complete, Roche), and proteins were separated on 10% SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequencing libraries were prepared with the HyperPrep Kit (KAPA BIOSYSTEMS), from 1ug DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin-containing RNA probes were generated by in vitro transcription with T7 polymerase using the DIG RNA Labelling kit (SP6/T7) from Roche (cat. No 11175025910). The oligonucleotide AJ529 (T7 promoter ...
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bioRxiv - Microbiology 2023Quote: ... and 2 mM b-mercaptoethanol (BME)] in the presence of an anti-protease cocktail (Complete EDTA-free, Roche) and 1 μl of benzonase (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the bacterial pellets were resuspended in TBS (20 mM Tris + 150 mM NaCl) plus cOmplete mini EDTA free protease inhibitors (Roche) and disrupted by bead beating four times for 30 seconds with zirconia beads (BioSpec Products) ...
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bioRxiv - Microbiology 2023Quote: ... The ERIC-PCR was performed with 25-μL volumes containing Taq DNA polymerase buffer 1X (Roche), 200 mM dNTP (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... and Sequencing Grade Chymotrypsin (Roche) at an enzyme-to-substrate weight ratio of 1:25 for 3 hours and then overnight at 37 °C in 100 mM phosphate buffer pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... 20% glycerol) with protease and phosphatase inhibitors (Roche). Cells were lysed using a Bead Beater and 300μl of glass beads ...
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bioRxiv - Microbiology 2023Quote: ... cells were harvested then resuspended in lysis buffer (25 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol) supplemented with cOmplete™ EDTA- free protease inhibitors (Roche). Cells were lysed using a Dounce homogeniser followed by sonication on ice (60% amplitude ...
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bioRxiv - Microbiology 2023Quote: ... with the MagNA Pure 96 Cellular RNA Large Volume Kit (Roche), using the RNA tissue FF standard cellular RNA protocol with an elution volume of 50 μL per sample ...
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bioRxiv - Microbiology 2023Quote: 20 μL heparinised blood was added to 400 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer (Roche). Head kidney pieces (3 mm × 3 mm ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted on a MagNA Pure 96 instrument (Roche) with the MagNA Pure 96 Cellular RNA Large Volume Kit (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... with cycling conditions following manufacturer’s instructions (Table S5, SI1) using the Light Cycler 480 (Roche Applied Science) in a 384-well plate format (Roche Applied Science ...
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bioRxiv - Microbiology 2023Quote: ... in a 384-well plate format (Roche Applied Science, Cat. No. 04729749001). All assays were performed in technical triplicates where pre-amplified DNA from technical triplicate was used as a template for independent monoplicate qPCR (allowed by high repeatability between technical triplicates in qPCR) ...
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bioRxiv - Microbiology 2023Quote: 10-25ml of bacterial cultures were pelleted and resuspended in extraction buffer (50mM Tris-HCl pH 7.5, 5mM EDTA, and 6% SDS) with protease inhibitor cocktail (Roche; 1mg/ml Aprotinin ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM MgCl) with with cOmplete Protease Inhibitors Cocktail (PI, Roche Diagnostics, Rotkreuz, Switzerland) and homogenized at 4 °C using a pre-cooled Potter-Elvehjem PTFE pestle at 100 RPM and glass tube with a working volume of 30 mL ...
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bioRxiv - Microbiology 2023Quote: ... 10 μL DNAse I (Roche) 10 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... 10 µg/mL of DNAse I (Roche) and 10 µg/mL of RNAse A (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... or anti-HA affinity matrix beads (Roche) was rinsed four times with 1 mL TES buffer and centrifugation 2 minutes at 3000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... and treated for 30 min at 37°C with 20 U/ml DNase I (Roche). Virus was pelleted through a 25% sucrose cushion ...
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bioRxiv - Microbiology 2023Quote: ... 100 mM NaCl) containing protease inhibitor (cOmplete tablets, EASYpack from Roche) at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen ...
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bioRxiv - Microbiology 2023Quote: ... and pre-cleared with 80 μl of Protein-A agarose (Roche, www.roche.com) and 100 μg BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... with FastStart Essential DNA Green Master kit (Roche; Rotkreuz, Switzerland) with ~3 ng of cDNA and three technical replicates ...
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bioRxiv - Molecular Biology 2023Quote: ... with 100 ng RNA per sample on a LightCycler 96 instrument (Roche). PCR primers for Rpl8 and Ifna4 were used as given above together with the universal probe library probes (UPL ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Molecular Biology 2023Quote: ... reduced with 10 mM DTT (Roche, 10197777001) for 45 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... adjusted to pH=6.5) supplemented with Complete Protease inhibitor cocktail (Roche), Complete Phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... GluC (Roche, Mannheim, Germany), LysC (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was done using the KAPA HyperPrep Kit (Roche, KK8504) with 4 μL of Illumina adapters (IDT ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was determined by the TUNEL assay using the In Situ Cell Death Detection Kit Fluorescein (Cat # 11684795910; Roche, Germany); only a signal overlapping a DAPI-stained nucleus was considered positive ...
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bioRxiv - Molecular Biology 2023Quote: ... using the KAPA SYBR® FAST Universal qPCR Master Mix (2x; Kapa Biosystems, Inc., USA). Relative mRNA levels were normalized to 18S expression levels via the ΔΔ method as previously reported (Pérez-Estrada et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were transfected into HEK293T cells using X-tremeGENE 9 (Roche # XTG9-RO) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 250µL bacterial Lysis Buffer (Roche) was added to 250µL bacterial suspension ...
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bioRxiv - Microbiology 2023Quote: ... 1x protease inhibitor cocktail (Roche)) ...