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Citations for Roche :
651 - 700 of 5641 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-PCR was performed with 20 ng cDNA in a LightCycler instrument (Roche) using FastStart Essential DNA Green Master (Roche 06402712001) ...
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bioRxiv - Molecular Biology 2022Quote: ... 300 ng of total RNA was reverse-transcribed in 20 μl using Transcriptor (Roche) primed with 150 pmol random hexamers following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... We amplified 10 ng of array oligos with KAPA HiFi 2x Readymix (Kapa Biosystems) with a 65 °C annealing temperature and 30 s extension ...
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bioRxiv - Neuroscience 2023Quote: ... QPCR was performed (800 ng RNA) using a LightCycler® 480 instrument (Roche Diagnostics) with the following primers (300 nM) ...
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bioRxiv - Neuroscience 2023Quote: ... QPCR was performed (200 ng RNA) using a LightCycler® 480 instrument (Roche Diagnostics) with the following primers (5’ to 3’ at 300 nM) ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2020Quote: ... 25 μg/ml gentamicin] containing 75 U/ml collagenase and 20 μg/ml dispase (Roche) with gentle shaking at 37°C until isolated crypts could be observed microscopically ...
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bioRxiv - Neuroscience 2021Quote: ... anti-HA rat (Roche, 11867423001; 1:100), anti-Brp mouse (nc82 ...
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bioRxiv - Microbiology 2019Quote: ... anti-GFP mouse 1:100 (11814460001; Roche), anti-HA rabbit 1:50 (H6908 ...
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bioRxiv - Plant Biology 2020Quote: ... 1:100 cOmplete protease inhibitor cocktail (Roche). A small aliquot of samples in co-IP buffer was used for input control before adding α-GFP agarose beads ...
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bioRxiv - Neuroscience 2021Quote: ... and rat anti-HA (1:100, Roche). Secondary antibody used were goat anti-Rabbit Alexa 488 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-HA (Roche, 11867423001; 1:100), mouse anti-Brp (nc82 ...
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bioRxiv - Immunology 2020Quote: ... and liberase TM (1:100, 5401127001, Roche) for 30 min at 37°C under agitation ...
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bioRxiv - Cell Biology 2021Quote: ... mouse GFP (11814460001, Roche, IF: 1/100); mouse HA (32-6700 ...
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bioRxiv - Cell Biology 2020Quote: ... and phosphostop (1:100, Roche, Basel, Switzerland). 30μg of protein was dissolved in sample-loading buffer (5% SDS ...
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bioRxiv - Biochemistry 2019Quote: ... 1% TritonX-100 and protease inhibitors (Roche)) and sheared following addition of 0.5% NP-40 ...
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bioRxiv - Molecular Biology 2019Quote: ... and 100 μL of HA agarose (Roche) were added ...
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bioRxiv - Developmental Biology 2019Quote: ... HA-epitope-tag (Roche, rat, 1:100), Discs Large (DSHB ...
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bioRxiv - Cell Biology 2021Quote: ... rat anti-HA (1:100, Roche 3F10), rabbit anti-cleaved caspase 3 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-HA (Roche 3F10, 1:100), mouse anti-CaMPARI-red (Absolute antibody 4F6 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25% Triton X-100 (Roche, Cat#11332481001), 0.2% Glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µM random hexamer primers (Roche # 11034731001) and 1 μl RNAsin Plus Inhibitor (Promega #N2615 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:100 Protease Inhibitor Complete (Roche; 1183617000)) ...
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bioRxiv - Cell Biology 2021Quote: ... 6×106-1.2×107 HL-60 cells were lysed in RIPA Lysis and Extraction buffer (#89900, Thermo ScientificTM) according to manufacturer’s instruction with supplementation of the protease inhibitors (#4693159001, Roche). Samples were denatured with 4xLaemmli buffer (#161-0747 ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were lysed with 400 μL of NAD+/NADH extraction buffer containing a cocktail of protease inhibitor (Roche Diagnostic ...
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bioRxiv - Plant Biology 2020Quote: ... Cell pellets from 200 mL of Chlamydomonas culture were resuspended in an extraction buffer (20 mM HEPES pH= 7.5, 20 mM KCl, 10% glycerol, 2× EDTA-free protease inhibitor mix (ROCHE)) ...
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bioRxiv - Plant Biology 2021Quote: ... and 250 µl extraction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 % Nonidet P40, proteinase inhibitor cocktail (Roche)) per 200 mg tissue powder was added ...
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bioRxiv - Cancer Biology 2020Quote: Organoids were incubated in Cell Recovery Solution and nuclear extracts were prepared by resuspending organoids in nuclear extraction buffer A (10 mM HEPES/KOH pH 7.9, 0.1 mM EDTA, 10 mM KCl, 1x Complete® [Roche, 1697498] ...
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bioRxiv - Cell Biology 2021Quote: ... After addition of the extraction solution containing 1% acetic acid and Complete Mini protease inhibitor cocktail (Roche, Basel, Switzerland) in 1:2 w/v proportion ...
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bioRxiv - Plant Biology 2019Quote: ... Seedlings were ground in liquid nitrogen and homogenized in ice-cold extraction buffer (25 mM HEPES-KOH pH 7.5, 10 mM MgCl2, protease inhibitor cocktail [complete Mini, Roche] ...
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bioRxiv - Molecular Biology 2019Quote: ... washed twice in ice-cold acetone then resuspended in 200 μl ice-cold extraction buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 1x protease inhibitors (Complete EDTA-free, Roche), 1% SDS) ...
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bioRxiv - Neuroscience 2020Quote: Proteins were extracted using Tris-buffered Saline (TBS) with 0.5% NP40 protein extraction buffer containing protease and phosphatase inhibitors (Roche), on ice ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: Cells were lysed using RIPA lysis and extraction buffer with protease inhibitors and phosphatase inhibitors from Roche (Indianapolis, IN). Protein concentration were determined with the Bio-Rad protein assay (Hercules ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction was carried out using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche, Diagnostics) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and vortexed vigorously in extraction buffer 1 (50 mM Tris–HCl pH 7.5, 150 mM NaCl, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Microbiology 2023Quote: 10-25ml of bacterial cultures were pelleted and resuspended in extraction buffer (50mM Tris-HCl pH 7.5, 5mM EDTA, and 6% SDS) with protease inhibitor cocktail (Roche; 1mg/ml Aprotinin ...
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bioRxiv - Genomics 2023Quote: DNA extraction for sequencing with Illumina was done using High Pure PCR Template Preparation Kit from Roche (Mannheim, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: Embryonic lungs at E13.5 were collected in RIPA Lysis and Extraction Buffer (Thermo) supplemented with cOmplete Protease Inhibitor Cocktail tablets (Roche). Samples were homogenized in a QIAGEN TissueLyser II and proteins were purified from the supernatant after high-speed centrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were lysed by sonication in extraction buffer (50 mM Hepes pH 7.5, 50 mM NaCl, 1x protease inhibitor cocktail (EDTA-free cOmplete, Roche) and the His-tagged proteins were purified with HisBind NiNTA-agarose (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... transient transfection was performed with a total of 1 µg of plasmids (EGFRmCitrine, PTBmCherry and cCblBF P at ratio 4:3:4 by mass) using FUGENE6 (Roche Diagnostics) transfection reagent and Opti-MEM (Gibco - Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and 4-nitroblue tetrazolium chloride (NBT) (Roche Diagnostics). After washing ...
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bioRxiv - Microbiology 2022Quote: ... 0.85 mg/ml; Collagenase D, Roche, 11088882001, 1.25 mg/ml; dispase, Gibco, 17105-041, 1 mg/ml; DNase, Roche, 101104159001, 30µg/ml). Tissues were then incubated at 37 °C with shaking for at least 15 min and manually shaken every 5 minutes until complete digestion ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... pH 8.0, 100 mM NaCl, 5mM MgCl2, 1% Triton X-100, 1mM PMSF, and complete protease inhibitor cocktail; Roche) and lysed twice with high pressure (1200 psi ...
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bioRxiv - Genomics 2020Quote: ... and mixed with 100 μL Binding Buffer (1% Triton X-100, 0.1% Sodium Deoxycholate, 1x complete protease inhibitor (Roche)) plus 100 μL 0.2 μg/μl chromatin followed by overnight incubation on a rotating platform at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed in ice-cold PBS and lysed in lysis buffer (100 mM TrisHCl pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitors (Roche)) for 30 minutes on ice ...