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Citations for Roche :
801 - 850 of 5641 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 0.5% Triton X-100, 100 mM NaF, 1 mM ortho-vanadate, 2 mM EDTA, and a protease inhibitor cocktail [Roche 11836153001]). Lysates were vortexed and cleared by centrifugation (15,000 x g for 15 min at 4°C) ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were lysed with 5 mL nuclear lysis buffer (60 mM HEPES pH 7.5, 10 mM MgCl2, 100 mM KCl, 0.1% Triton X-100, 10% glycerol, Roche cOmplete protease inhibitor) and sonicated for 8 cycles with 10 s on and 50 s off16 ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.2 mg/mL collagenase with 60 U/mL DNase I (Roche). At the end of each round of digestion ...
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bioRxiv - Immunology 2020Quote: ... and digested in 0.34 U/ml Liberase TM (0.06mg/ml, Roche) as previously described for 60 minutes at 37°C with manual agitation every 15 minutes (Zychlinsky Scharff et al. ...
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bioRxiv - Immunology 2020Quote: ... 10μg/ml DNase I and 1.5mg/ml dispase II (all Roche). The LP lymphocyte-enriched population was harvested from a 40%-80% Percoll (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mL human insulin (final concentration 20 μg mL-1, Roche), 1ml BSA (final concentration 50 μg mL-1 ...
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bioRxiv - Immunology 2022Quote: ... 2 ml of DMEM with 0.26 U ml−1 LiberaseTM (Roche) and 0.25 mg ml−1 DNase I (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µg/ml streptomycin and 0.4 mg/ml Liberase™ (Roche). The tissue digest was stopped by addition of an equal volume of RPMI supplemented with 10% heat- inactivated FCS ...
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bioRxiv - Immunology 2022Quote: ... Another 1 ml DMEM medium containing 0.625 mg/ml DNase1 (Roche) and 0.26 U Liberase DH (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... The culture supernatant for each condition was harvested for RNA extraction using the HP Viral RNA Kit (Roche, Cat no. 11858882001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... three-to-four 5μm FFPE samples sections were taken for RNA extraction using commercial FFPE nucleic acid isolation kit (Roche Molecular Diagnostics). RNA was quantified using Nanodrop and analyzed for fragment distribution using a bioanalyzer (Agilent) ...
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bioRxiv - Physiology 2021Quote: ... Collagen was then extracted from the tissue samples using a salt extraction buffer (1 M NaCl, 25 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease inhibitors (Roche, Basel, Switzerland). Samples were extracted overnight at 4°C with agitation ...
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bioRxiv - Neuroscience 2019Quote: ... Automated purification of genomic DNA was conducted using a MagNA Pure 96 machine and commercial extraction kits (Roche Diagnostics, Mannheim, Germany). Five common OXTR SNPs were chosen based on previous studies suggesting associations with social behavior (OXTR rs237887 ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated protein was incubated with 50ug fresh mitochondria extraction in import buffer containing ATP regeneration system (Creatine kinase (Roche 10127566001), Creatine Phosphate (Sigma-Aldrich 10621714001 ...
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bioRxiv - Microbiology 2022Quote: ... Following DNA extraction Illumina shotgun sequence libraries were prepared using the Kapa HyperPrep kit according to manufacturer specifications (Roche; Basen, Switzerland). Libraries were sequenced on an Illumina NovaSeq S4 flow cell (Illumina ...
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bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
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bioRxiv - Microbiology 2023Quote: RNA from specimens from Cyprus underwent automated total nucleic acid extraction using the MagNA Pure 96 DNA AND Viral NA Small Volume kit (Roche Diagnostics). RT-PCR for FCoV was performed at Laboklin Bad Kissingen ...
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bioRxiv - Developmental Biology 2024Quote: ... the tail of each embryo was collected for genomic DNA extraction using the KAPA Mouse Genotyping Kit HotStart (Kapa Biosystems, KK7352). Subsequently ...
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bioRxiv - Cancer Biology 2019Quote: ... and 20 ng of cDNA template was amplified on a LightCycler-480 (Roche, Indianapolis, IL, USA) using a FAST SYBR Green Master Mix (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... Libraries were prepared using 500 ng of fragmented gDNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Agilent SureSelect XT Target Enrichment System and Human All Exon V5 capture baits (Agilent Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng of genomic DNA was used for individual libraries using a KAPA HyperPlus Kit (Roche Sequencing Solutions ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries (500 ng total RNA) were prepared using the KAPA Hyperprep mRNA-Seq Kit (KAPA Biosystems). cDNA fragments were ligated to indexed library adapters (Illumina ...
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bioRxiv - Genetics 2022Quote: ... 1μg of BAC were incubated for nick-translation with 4.3 ng of DNAse I (Roche, 104159), 7U of DNA polymerase (Promega ...
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bioRxiv - Microbiology 2022Quote: ... The pooled library concentration was calculated by using the NGS Library Quantification Complete kit (Roche 07960204001) and then loaded on a Miseq sequencer ...
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bioRxiv - Cancer Biology 2022Quote: 150 ng of total DNA was used for library preparation using the KAPA HyperPlus kit (Roche), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were quantified by real-time PCR using KAPA Library Quantification Kit for NGS (Roche) and assessed for size distribution on a Fragment Analyzer (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR reactions were carried out on 0.625 ng of cDNA using a LightCycler 480 System (Roche), with the SYBR® Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 ng analysed by qPCR using a Light Cycler 480 real-time PCR system (Roche) to quantify the proportion of each tagged strain.
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bioRxiv - Cell Biology 2023Quote: ... and pLentiCRISPRv2-PFN1sgRNA (500 ng each) into HEK293FT cells using X-tremeGENE HP transfection reagent (Roche). Lentivirus was harvested 72 hours later and subsequently used to infect JR20 cells supplemented with 4 μg/mL of Polybrene ...
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bioRxiv - Immunology 2022Quote: ... and lung were further digested in RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche) on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with 4-Nitroblue tetrazolium chloride (NBT, Roche) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Neuroscience 2020Quote: ... and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, Roche) in NTMT pH9.5 solution (100mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, #11383221001, Roche) and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT ...
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bioRxiv - Neuroscience 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (Roche Diagnostics) in detection buffer ...
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bioRxiv - Biophysics 2020Quote: ... 4 mM digitonin and EDTA-free protease inhibitor tablets (Roche). Insoluble material was removed by ultracentrifugation at 100,000 g for 30 min ...
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bioRxiv - Physiology 2019Quote: ... 4 Pefabloc (Roth) and Complete® proteinase inhibitor cocktail (Roche). Cell debris were removed by centrifugation at 1000 g for 10 min and the supernatant was ultracentrifuged at 269,000 g for 30 min ...
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bioRxiv - Physiology 2019Quote: ... 4 Pefabloc (Roth) and Complete® proteinase inhibitor cocktail (Roche). Protein concentration of the membrane fractions was determined by BCA.
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bioRxiv - Physiology 2020Quote: ... 4 µL of 0.25 µg/µL trypsin (Roche, sequencing grade) was added to each sample and left overnight at 37 °C.
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bioRxiv - Molecular Biology 2020Quote: ... and then with DAPI (4′,6-Diamidino-2-phenylindole, Roche) for 15 min ...
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bioRxiv - Immunology 2019Quote: ... grade II and 4 mg collagenase A (Roche Applied Science) and placed in a 37°C water bath for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... #11383213001)/BCIP (5-bromo-4-chloro-3-indolyl phosphate, Roche, #11383221001) color development substrate via AP activity ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate solution (Roche). Images were captured using a BX-50 microscope (Olympus Corp. ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained using the Red Counterstain II kit (4 min, Roche), removed from the automated system and rinsed with distilled water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed 5 μl of crude lysate with 45 μl of TNG buffer (100 mM Tris, 100 mM NaCl, 10% glycerol, 10 mM DTT, 1×cOmplete™ (EDTA-free Protease Inhibitor Cocktail, Roche 11873580001), pH 7.2-7.5) ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were lysed in Tris-lysis buffer (10 mM Tris-Cl pH 7.5, 100 mM NaCl, 1% Triton X-100, 10% glycerol, and cOmplete™ protease inhibitor cocktail [Roche, Basel, Switzerland]) on ice ...