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Citations for Roche :
501 - 550 of 5641 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 102 ng/μl creatine kinase (Roche, Cat. No. 10127566001), 0.4 mM amino acid mixture ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) with 400 ng of anti-HA (Roche), together with 10 μL protA and 10 μL protG Dynabeads suspension (ThermoFisher) ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... 18.75 mg/ml nitro blue tetrazolium chloride, 9.4 mg/ml 5-bromo-4-chloro-3-indolyl phosphate toluidine salt in 67% dimethyl sulfoxide, Roche; Cat. No. 1681451) was added to the third change of NDB while stirring thoroughly until completely dissolved ...
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bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell pellets were lysed in 300 µL of lysis buffer (10 mL BugBuster® [Millipore 70584-4], ¼ tablet cOmplete™ Protease Inhibitor Cocktail EDTA-Free [Roche]) for 20 min at room temperature and centrifuged at 13,000 rpm at 4℃ for 30 min to clear all cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 500 x g for 5 min at 4°C and resuspended in 10 ml 4 °C RSB supplemented with protease inhibitor (cOmpletetm, EDTA-free protease inhibitor cocktail, Roche, Cat#: 11873580001) and incubated on ice for 15min ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining tissue was digested on a shaker at 400 rpm for 40 min at 37°C in 100 μg/ml Liberase TM (Roche, 5401127001) and 100 μg/ml DNAse I (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM EDTA, 0.5 mM DTT, and 0.5% NP40 substitute supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail) and sonicated for 5 minutes using BioRuptor Pico water bath sonicator (30 s ON/OFF ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 volume of nuclei suspension was mixed with 1 volume of DNase I cocktail (NWB supplemented with CaCl2 2 mM, BSA 100 μg/ml, and twice the indicated concentration of DNase I (Roche 10104159001) and incubated for 8 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: Cancer transplantation was performed as described in the section “spatial transcriptomics.” The obtained livers were minced and dissociated in RPMI1640 containing DNase I (100 μg/ml: Roche, Switzerland) and collagenase IV (1 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 0.6 mL of incubation buffer (citric acid 100 mM and NaCl 250 mM pH 5.5 with inhibitor cocktail (Roche, Rotkreuz, Switzerland) dilution according to the instructions of the manufacturer) ...
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bioRxiv - Genetics 2023Quote: ... cells were resuspended in 3 ml lysis buffer (PBS containing 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride and cOmplete proteinase inhibitor [Roche Diagnostics]), lysed by ultrasonic treatment and incubated with 1.2 ml NeutrAvidin Agarose for 0.5 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: KPC-4662 tumors from sedentary and exercised mice were harvested and immediately digested in HBSS with DNase I (100 µg/mL; Roche, 04716728001) and collagenase type II (10mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... as the internal reference plasmid were co-transfected with the P65 and P50 expression vector pAd5 E1-CMV-P65/P50 (each 300 ng) or the control vector pAd5 E1-CMV-MCS (600 ng) into the cells using X-tremeGENE HP Reagent (Roche, Indianapolis, IN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... and hematoxylin (4 minute incubation) and Bluing Reagent (4 minute incubation) counterstain (Roche, Ventana Medical Systems ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellet samples were collected and re-suspended in lysis buffer (100 mM Tris-HCl, pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set ...
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bioRxiv - Microbiology 2020Quote: ... and Cobas Omni LYS used for on-board lysis by Cobas extraction platforms (Roche); Viral PCR Sample Solution (VPSS ...
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bioRxiv - Microbiology 2021Quote: RNA from virus was extracted using High Pure Viral Nucleic acid extraction kit (Roche). Using gene specific reverse primers of target genes ...
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bioRxiv - Microbiology 2022Quote: ... Specific DNA fragments were isolated using an agarose gel extraction kit (Roche Applied Science). Restriction enzyme digests were carried out using enzymes purchased from New England Biolabs by following the manufacturer’s recommended procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... We then prepared libraries from these extractions with a Hyper library kit (Kapa Biosystems) and sequenced them on an Illumina NovaSeq 6000 with 150bp paired-end reads [32] ...
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bioRxiv - Cell Biology 2023Quote: ... We used identical input total RNA (50 ng to 500 ng) between control and samples for library preparation using the KAPA RNA HyperPrep Kit (KAPA Biosystems, Wilmington, MA, USA). Libraries were analyzed by Agilent 2100 bioanalyzer system for quality control ...
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bioRxiv - Biophysics 2024Quote: ... to a final molal concentration of 25 ng/μL and amplified using primers specific to each oligonucleotide subpool with KAPA SYBR FAST qPCR (KAPA Biosystems; 1-ng template). A second PCR amplification was performed with KAPA HiFi (KAPA Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... Cell-pellets were resuspended in 25 ml PBS and incubated for 30 min at 4 °C in the presence of 1 mg/ml Lysozym and protease inhibitors (Roche Diagnostics, Penzberg, Germany) The cell lysate was cleared by centrifugation at 40000 g for 10 minutes after sonification with a Bandelin Sonapuls HD 2070 (Reichmann Industrieservice ...
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bioRxiv - Immunology 2022Quote: ... using 1000 ng of RNA per sample (Roche, Basel, Switzerland). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2021Quote: ... and qPCR using an NGS Library Quantification Kit (KAPA Biosystems) prior to normalisation ...
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bioRxiv - Biochemistry 2020Quote: ... using 10 ng / µl yeast tRNA as a carrier (Roche) and created NTC samples of the same dilution ...
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bioRxiv - Plant Biology 2019Quote: ... and qPCR using an NGS Library Quantification Kit (KAPA Biosystems) and then normalised ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were digested with 200 ng trypsin (Roche, Basel, Switzerland) shaking at 600 rpm at 37°C for 17 hours ...
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bioRxiv - Microbiology 2023Quote: ... destained and digested with Asp-N (Roche, 20 ng/μL) overnight at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and in-gel digested with 10 ng/μl trypsin (Roche).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5 mM imidazole) supplemented with 100 µg/mL of lysozyme and a protease inhibitor cocktail (Complete, mini, EDTA-free tablet, Roche Applied Science) then incubated with shaking for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated by isopropanol precipitation of cells and tumor lysates (lysis buffer was Tris [pH 8] 100 mM, EDTA 5 mM, SDS 0.2%, NaCl 200 mM, and 1 mg/mL proteinase K [Roche Diagnostics, Mannheim, Germany]) and resuspended in Tris/EDTA (TE ...
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bioRxiv - Developmental Biology 2020Quote: ... 4-toluidine salt (BCIP, Roche) in NTMT buffer ...
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bioRxiv - Microbiology 2023Quote: ... 4 U DNase I (Roche) with the reaction buffer provided with the enzyme for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 mM MgCl2 (Roche Diagnostics), 0.3 µM of each primer (RB1_80F and RB1_235R ...
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bioRxiv - Biochemistry 2020Quote: ... Triton X-100 (Roche), IgG-Agarose resin (GE Healthcare) ...
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bioRxiv - Genomics 2022Quote: ... 100 units DNase1 (Roche) was added ...
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bioRxiv - Developmental Biology 2020Quote: ... GFP (Roche, 1/100) and pH3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... NimbleGen 4-plex arrays containing 4 × 72,000 arrays per slide were used (Roche, Mannheim, Germany). Construction of this chip was initially based on combining two previous genome annotations (Amselem et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μg of plasmid and 4 μl of X-tremeGENE HP DNA Transfection Reagent (Roche) were used per well ...
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bioRxiv - Developmental Biology 2024Quote: ... NBT/BCIP (4-nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolylphosphate, Roche, 11681451001) was added after thoroughly washing samples ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS containing 0.5% Tween-20 samples were incubated with fluorescent labelled secondary antibodies containing 4′,6-diamidino-2-phenylindole (DAPI; Roche Cat# 10236276001, 1.0 µg/ml) for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... RNA extraction was performed using the Roche High Pure Viral RNA Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cryomilled cellular materials were resuspended extraction buffers containing a protease inhibitor cocktail without EDTA (Roche), and clarified by centrifugation (20,000 x g ...
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bioRxiv - Plant Biology 2022Quote: ... The plant leaves were ground in liquid nitrogen and extracted in extraction/washing buffer (Roche). The extraction proteins were eluted with 1.5×Laemmli loading buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The triton extraction buffer was supplemented with a cOmplete™ Protease Inhibitor Cocktail (Roche; #11697498001) and PhosSTOP™ (Roche ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cultures were lysed in M-PER mammalian protein extraction reagent supplemented with PhosphoSTOP (Roche) and cOmplete Tablets (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested and resuspended in binding buffer (100 mM Tris- Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, complete protease inhibitor cocktail (Roche; 1 tablet/10 mL) before sonicating on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...