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Citations for Roche :
651 - 700 of 8790 citations for 1 3 Nitro 10 11 dihydro 5H dibenzo b f azepin 5 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the signal was visualized by incubation with nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP) solution (Roche Diagnostics, 11681451001) in 0.1 M Tris-HCl (pH9.5 ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were developed in a NBT/BCIP solution (nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indoxyl phosphate; Roche; Basel, CH) for up to 20 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were developed in a NBT/BCIP solution (nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indoxyl phosphate; Roche; Basel, CH) for up to 20 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ∼ 200 embryos of 1dpf were placed in 1mL modified Ringer’s solution (116 mM NaCl, 3 mM KCl, 4 mM CaCl2, 5 mM HEPES; pH 7.5) containing Protease Inhibitor (Roche, Cat. No. 04693132001) and Phosstop (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... was annealed with Coccus-R primer (5′–ACG– TCA–GAA–TCG–CTG–C–3′) and analyzed using FastStart Essential DNA Green Master kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed 3 times with 10 ml of ice-cold PBS supplemented with Complete Protease Inhibitor (Roche, Basel, Switzerland) and cells were resuspended in 1 ml of ice-cold PBS supplemented with Complete Protease Inhibitor ...
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bioRxiv - Molecular Biology 2022Quote: ... Indexes and Illumina cluster generation sequences were then added with a secondary PCR reaction using 3 μL of the diluted primary PCR product with a 10 μL Kapa Robust HotStart polymerase reaction (Roche) for 20 cycles ...
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bioRxiv - Bioengineering 2024Quote: All qPCR reactions were carried out using 384-well plates in 3 technical replicates in 10 µl final reaction volume on the LightCycler 480 System II (Roche). Reaction mix consisted of Power Track SYBR Green Master Mix 2X ...
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Impact of light pollution at night on male reproductive success in Japanese medaka (Oryzias latipes)bioRxiv - Zoology 2023Quote: ... Each cDNA sample was measured in duplicate using 3 μL of 4x diluted cDNA per 10 μL reaction in a LightCycler96 Instrument (Roche). qPCR parameters were set to 10 minutes of preincubation at 95 °C ...
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bioRxiv - Genomics 2024Quote: ... Nuclei were isolated by carefully transferring the extracted parasite mixture on top of a 0.25 M to 0.1 M sucrose gradient in cell lysis buffer (10 mM Tris pH 8, 3 mM MgCl2, 0.2% NP-40, 1x EDTA free Protease inhibitor (Roche, 04693132001); 15 mL 0.25 M Sucrose and 17.5 mL 0.1M Sucrose for 50 mL tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... uteri were harvested from pregnant mice at 4.5 days post coitus and washed with cold swelling buffer (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 1X Protease Inhibitor Cocktail (PIC, Roche, 11836170001)) immediately after collection ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x protease inhibitor cocktail (cOmplete ULTRA Tablets, Mini, EDTA-free, Roche; 1:10), pH 8.5 ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg/ml-1 DNase I and 1 protease inhibitors (cOmplete EDTA-free, Roche) for each gram of pellet ...
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bioRxiv - Biochemistry 2021Quote: ... 137.5 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM sodium fluoride, EDTA-free protease inhibitor cocktail [Roche]). 1 mM orthovanadate was added to the lysis buffer to prevent binding of substrates to the PTP1B trapping mutant ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Genetics 2020Quote: ... 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml, ½ Roche phosSTOP tablet/10 ml) using 14 × 1.5 min bursts on a BioSpec mini bead-beater at room temperature ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... The NTP labeling mix contained either DIG-11-UTPs (DIG RNA Labeling Mix, Roche; Cat. No. 1277073) for synthesis of DIG-labeled probes or Fluorescein RNA Labeling Mix (Roche ...
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bioRxiv - Genomics 2020Quote: Northern probes were first synthesized using the PCR DIG Probe Synthesis Kit (Roche # 11 636 090 910). The 512 bp frq probe was amplified from wild-type Neurospora genomic DNA with primers ...
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bioRxiv - Genomics 2020Quote: ... subsequent washes were performed using the DIG Wash and Block Buffer Set (Roche # 11 585 762 001). Anti-Digoxigenin-AP Fab fragments were used at 1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... for Kcns3 and Kcnb1 probes or in the presence of digoxigenin (DIG)-11-UTP (Roche, Mannheim, Germany) for Pvalb probe ...
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bioRxiv - Cell Biology 2021Quote: ... Assay was performed according to protocol in Cell proliferation kit II (XTT) (Roche, #11 465 015 001).
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bioRxiv - Developmental Biology 2022Quote: ... DNA probes were labelled by nick translation with either biotin-16-dUTP or digoxigenin-11-dUTP (Roche). For simultaneous IF and RNA FISH ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The labelling nucleotide mix contained either biotin-16-dUTP or digoxigenin-11-dUTP (both Roche, Mannheim, Germany). Due to its long size ...
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bioRxiv - Genomics 2021Quote: ... probes were generated by PCR incorporation of DIG-11-dUTP into target sequences following manufacturer’s instructions (Roche). Chromosome 5-chromosome 7 translocation was detected using primers AB1028 and AB1029 amplifying a 180 bp region of chromosome 5 (Chr5 nt ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoblotted with a mouse monoclonal anti-HA antibody (clone 12CA5; Roche, 11 583 816 001, RRID:AB_514505). Rabbit monoclonal anti-PSTAIR (anti-Cdc2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then incubated overnight at 4 °C with α-DIG antibody (#11 093 274 910; Roche). After washing with TBST for 30 min × 3 times ...
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bioRxiv - Plant Biology 2022Quote: ... 200 mM NaCl, 1 mM EDTA, 1%NP-40, 1 mM DTT, 10 mM MgCl2, 1 × protease inhibitor cocktail from Roche) for 2 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... 1 M sucrose, 5 mM KCl, 5 mM MgCl2, 0.6% Triton X-100, 0.4 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 5 ml lysis buffer was made up using 5 ml RIPA buffer with 5 μl 1 M PMSF and 1x EDTA-free protease inhibitor cocktail (Roche, 11836170001). Cells were washed with 1 ml cold PBS before 250 μl lysis buffer was added to each well ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lumbar vertebrae 1 – 5 were incubated in 2% Collagenase P (Roche, Switzerland) for 30 minutes at 30° C ...
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bioRxiv - Biochemistry 2020Quote: ... 5% glycerol) supplemented with 1 Complete Protease Inhibitor (EDTA-free) tablet (Roche), 70 µg RNAse A (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... pH 7.6 with KOH) containing 5 mg mL−1 (type A, ROCHE). Oocytes were kept at 19 °C in a Barth’s solution (in mM ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100 and 5% glycerol) supplemented with protease inhibitor (Roche), 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen ...
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bioRxiv - Cancer Biology 2020Quote: ... NE were diluted 1:5 in IP buffer with protease inhibitor (Roche). NE was incubated overnight at 4°C on rotating wheel with 10 ug of G9a antibody (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... 1% Triton X-100 and 5% glycerol) supplemented with protease inhibitor (Roche), 1 μL/mL Ready-LyseTM Lysozyme Solution (Lucigen ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryos underwent blocking with 1:5-diluted Western Blocking Solution (Roche) for 1 hour at room temperature under agitation ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were blocked in Blocking Buffer (5% sheep serum with 10% Roche Blocking Reagent 10x in MABT) for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL DNA (10 ng/μL) were amplified using Lightcycler® FastStart DNA Master HybProbe Mix (Roche, Germany) and the respective LightSNiP Assay (TIBMolBiol ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei isolated from approximately 2.5x107 cells were resuspended in one ml of extraction buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM DTT, 0.25%NP-40, 1x Roche Complete protease inhibitors ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries from 5-10 ng of ChIP DNA was prepared using the KAPA HTP Library Preparation Kit (Roche) by the Next-Generation Sequencing Core at UT Southwestern ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were lysed with 5mL cold lysis buffer (10 mM Tris-HCl, pH 7.5; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; 1x complete protease inhibitor; 11836145001 Roche) for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5 mM N-ethylmaleimide and Complete protease inhibitor cocktail (Roche, one tablet/10 ml of buffer). HA-tagged ubiquitin was purified using Pierce anti-HA magnetic beads (clone 2–2.2.14) ...
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bioRxiv - Immunology 2020Quote: ... 10 mM NaF) containing 1% protease inhibitor cocktail solution (Roche). Cell lysates were clarified by centrifugation at 13000 rpm for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... an extra 1 µl of 10 mM dideoxynucleoside triphosphate (Roche) was added to replace 1 µl of nuclease-free water ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Tris pH 7.2 and 1% blocking solution (Roche) at 80°C ...
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bioRxiv - Microbiology 2020Quote: ... and a protease inhibitor cocktail (Roche; 1 tablet/10 mL)] on ice overnight ...
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bioRxiv - Immunology 2022Quote: ... and PhosSTOP™ (#4906845001, Roche, 1 tablet per 10 ml) immediately before use ...