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Citations for Roche :
6501 - 6550 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... resuspended in 4 ml of a buffer (HEPES 10 mM pH 7.4, 1 mM EDTA, EDTA-free protease inhibitor cocktail from a tablet (Roche)) and lysed by 3 passages through a cell disrupter at 2.4 kbar ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2) supplemented with a cocktail of protease inhibitors (CLAPA and 1 µg/mL cOmplete, EDTA-free, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Microbiology 2024Quote: ... Proteases were inactivated by the addition of 1 mM PMSF and 10 ul 1x complete protease inhibitor cocktail (Roche).
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bioRxiv - Neuroscience 2024Quote: ... cells were lysed in IPH buffer (20mM Tris-HCl pH7.5, 150mM NaCl, 2mM EDTA, 1% NP-40) supplemented with protease inhibitor cocktail (Roche, cOmpleteTM EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2024Quote: ... 1% NP-40 (vol/vol) supplemented with protease inhibitor cocktail (cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Cat# 05892791001) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in cold extraction buffer (50 mM Tris-HCl pH 7.5 containing 1 mM EDTA, 4 mM DTT, cOmplete Protease Inhibitor Cocktail (Roche), and 1 mM PSMF ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells are then washed twice in cold 1% BSA solution containing 0.5X EDTA-free Protease inhibitor cocktail (PIC, Roche), and once in cold PBS ...
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bioRxiv - Biochemistry 2024Quote: Cells were lysed in native lysis buffer (10% glycerol, 25 mM NaCl, 20 mM HEPES pH 7.0, 1% DDM) supplemented with protease inhibitor tablet (Roche), and solubilized by rotating 30 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with primary antibody for 2.5 h (GFP, Abcam, Cambridge, UK: rabbit, 1:10000; HA (Roche), rat ...
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bioRxiv - Physiology 2024Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNase from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Pellets were then homogenized with a Biospec Tissue Tearor (Dremel) ...
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bioRxiv - Plant Biology 2024Quote: ... The powdered tissue was then mixed with 1 ml of lysis buffer per gram of tissue (150 mM NaCl, 0.1% Triton X, and 50 mM TRIS HCl-pH 8, supplemented with 13:1 of Complete Mini, Roche).
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bioRxiv - Plant Biology 2024Quote: ... Aggregate fractionation buffer (100 mM HEPES pH 7.4, 1% Triton X-100, 300 mM NaCl) supplemented with Protease Inhibitor Cocktail (Roche) and 25 mM N-ethylmaleimide was added at 3 volumes per unit mass of dried seedling weight ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were washed in HBSS and then dissociated using 1 mg/mL collagenase and dispase (Roche, Indianapolis, IN, USA) for 45 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM pyridoxal 5’-phosphate hydrate (PLP) and proteases inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail Roche).
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bioRxiv - Molecular Biology 2024Quote: ... were pelleted and resuspended in NP-40 lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris-Cl pH 8.0) containing cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of RNA was converted into cDNA using the Transcriptor high-fidelity cDNA synthesis kit (Roche, Basel, Switzerland). The PCR mix contained 10 ng cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... The cell pellet was resuspended in Lysis buffer (20mM Tris-HCl pH 7.5, 150mM NaCl, 1% SDS, 1mM EDTA) containing protease inhibitor cocktail (Roche). The lysate was clarified by centrifugation (17,000xg for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... Brain stems were cut into pieces and digested for 40 min at 37 °C with 5% CO2 in PBS supplemented with 1.6 IU ml-1 Collagenase/Dispase (Roche) and 0.5 mg ml-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and centrifuged at 4000 rpm for 1 min at 4°C and run on the LightCycler 480 II (Roche) using the steps outlined in Supplemental Table 3.
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bioRxiv - Immunology 2024Quote: ... Tissues were then incubated for 1.5 hours at 37°C in an orbital shaker (200 rpm) with 1 ml of digestion media containing 0.25 mg/ml Liberase TL (Roche) and 0.25 mg/ml DNase I (Sigma ...
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bioRxiv - Immunology 2024Quote: ... brachial and mesenteric lymph nodes from 3 mice/genotype were pooled and cut into pieces and digested with the 1 ml of enzyme mix containing 0.8 mg/ml Dispase (Roche), 0.2 mg/ml Collagenase P (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were co-lysed together in lysis buffer (40mM Tris-HCl pH 7.5, 500mM NaCl and 1 mM DTT) with 100 mM Pefabloc (Roche) and protease inhibitor cocktail (Roche cOmplete EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer (80 mL per L of initial culture: 20 mM HEPES pH 8, 500 mM NaCl, 5% v/v glycerol, 0.5 mM TCEP + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
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bioRxiv - Cancer Biology 2024Quote: ... and MAZ (1:25, Immunoway Cat# YT2669) was performed using the Ventana BenchMark ULTRA instrument (Ventana/Roche, AZ, USA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... and the remaining cell pellet was lysed at a final concentration of 1.25×106 cells/mL in lysis buffer (1% NP40-PBS containing protease and phosphatase inhibitors (Roche)) and stored at -20°C until further analysis.
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 20 mM Imidazole, 1 mM TCEP, and protease inhibitors from Roche), and lysed by several passes through a microfluidizer (LM20 Microfluidizer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed twice with cold PBS before resuspending in ChIP shearing buffer (50 mM Tris HCl pH 8.0, 1% SDS, 10 mM EDTA with protease and phosphatase inhibitors, Roche). Samples were sonicated using a Covaris ME220 for 7.5 minutes using the following sonication conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody-bound beads were washed in BSA-PBS (0.5%) before the addition of pre-cleared lysate in 1% Triton with protease inhibitor (Roche). Following rotation overnight at 4 °C the beads were washed in RIPA (50 mM HEPES pH 7.6 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and resuspended in lysis buffer (50 mM HEPES pH 8.0, 300 mM NaCl, 1 mM TCEP, supplemented with Roche EDTA-free cOmplete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... islets were preincubated for 1 hour in KRBH buffer containing 0.1% (w/v) bovine serum albumin (BSA) (10775835001, Roche) and 3 mM glucose before incubation with 11 mM glucose ± 100 nM GLP-1RAs in KRBH buffer in a shaking 37°C water bath (80 rpm ...
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bioRxiv - Cancer Biology 2024Quote: Equal amounts of total RNA (1 µg) were reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with random hexamer primers for one hour at 50°C ...
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bioRxiv - Cell Biology 2024Quote: ... and thawing and by resuspension in 5 mL of ice-cold Lysis/Wash buffer (25 mM HEPES pH 7.5, 1 M NaCl, 5% glycerol) supplemented with cOmplete protease inhibitor cocktail (Roche), followed by vigorous sonication on ice delivered by a Jencons Ultrasonic Processor (15s bursts at 70% amplitude) ...
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bioRxiv - Cell Biology 2024Quote: ... The nuclear pellet was washed once with 3mL ST buffer and then twice with 3mL FANS buffer (1% BSA, 1mM DTT, and 0.2U/μL RNAse inhibitor (03 335 402 001, Protector RNAse inhibitor, Roche) in RNAse free DPBS (AM9624 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacteria pellets were resuspended in Lysis Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM DTT, 0.1% Triton-X, and protease inhibitors, cOmplete Mini, EDTA-free, Roche, #4693159001). Next ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris/HCl pH8) to which protease inhibitors were freshly added (1 tablet of Complete protease inhibitor per 25 ml, Roche, Mannheim ...
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bioRxiv - Cancer Biology 2021Quote: ... 150 mM NaCl, 1 mM PMSF, 1x protease inhibitor cocktail without EDTA from WAKO, 1x phosphatase inhibitor cocktail from Roche) containing 20 mM N-Ethylmaleimide to prevent further cysteine oxidation during protein extraction ...
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bioRxiv - Cell Biology 2020Quote: Cells were harvested using a cell scraper and were lysed with 200 μl/well of 0.1% CHAPS lysis buffer (50 mM Tris-HCl, 150 mM NaCl and 1 mM EDTA) supplemented with a Mini Protease inhibitor cocktail (Roche). The cell lysate was incubated on ice for 15 mins with occasional mixing before centrifugation at 20,000 g at 4°C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were allowed to air dry fully before applying Hybridizing Solution (70% formamide, 1 mg/ml blocking reagent (Roche), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Developmental Biology 2021Quote: Wildtype stain and drl:mCherry-positive zebrafish embryos were grown until tailbud stage and chorions were removed by incubating in 1 mg/mL Pronase (Roche), followed by washing in E3 medium ...
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bioRxiv - Genetics 2021Quote: ... livers were perfused at low flow for approximately 10 min with Digestion Buffer (low glucose DMEM supplemented with 1 mM HEPES) containing freshly added Liberase TM (32 μg/mL; Roche). Digestion was stopped using Isolation Buffer (low glucose DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and cells were washed twice in 1 ml Wash Buffer (20 mM HEPES-KOH pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1x Roche cOmplete™), followed by addition of 10 µl pre-activated concanavalin A coated magnetic beads (Bangs Laboratories-BP531) ...
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bioRxiv - Microbiology 2020Quote: Cell pellets of 1 × 106 cells were resuspended in 100 μl RIPA lysis buffer containing a protease inhibitor cocktail (Roche). Samples were centrifuged for 5 min to remove cell debris and stored at −20°C until use for Western blot analysis.
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bioRxiv - Molecular Biology 2021Quote: ... exponentially growing cells were treated with DMSO or 1 μM PARPi for 24 h followed by 45 min incubation at 37°C with Colcemid (Roche). Metaphase preparation and aberration analysis were performed as it follows ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... sections were incubated in a 1:1000 dilution of alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics, Cat. No. 11207733910) then stained with nitro blue tetrazolium (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% Tween 20) for 5 min each and then incubated in the AP reaction buffer with NBT/BCIP substrate (Roche).