Labshake search
Citations for Roche :
601 - 650 of 8573 citations for N' 4 Dimethylamino phenyl methyl 5 methyl 1 2 oxazole 3 carbohydrazide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... Mitochondrial pellets were solubilized in lysis buffer with 1 % digitonin (25 mM HEPES pH 7.5, 150 mM NaCl, 5 % glycerol, 1 mM TCEP, supplemented with PhosStop (Roche), cOmplete protease inhibitor cocktail EDTA-free (Roche) ...
-
bioRxiv - Microbiology 2023Quote: ... and resuspended in lysis buffer (50 mM PBS pH 7.4, 1 mM EDTA, 5% glycerol, 1 mM PMSF and protease inhibitors from Roche). Cell lysis and protein extraction was achieved through mechanical lysis using glass beads and Precellys®24 Homogenizer (3 cycles of 30 seconds at 5500 rpm ...
-
bioRxiv - Cell Biology 2024Quote: Cell pellet was collected and re-suspended in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol, Roche complete protease inhibitor ...
-
bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
-
bioRxiv - Plant Biology 2023Quote: ... pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % [v/v] glycerol, 1 mM DTT, and 1 × complete Protease Inhibitor [Roche; Cat. No. 058929700001]) was added to homogenised plant material (100 μl extraction buffer per 100 mg plant material) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1% SDS, 1 mM EDTA, 1 mM DTT, 1 mM Na3VO4×2H2O, 5 µM pepstatin A, 10 µM leupeptin, 2X Roche protease inhibitor cocktail), 200µl glass beads were added ...
-
bioRxiv - Neuroscience 2024Quote: ... rat anti-Elav (1:200) (DSHB), mouse anti-β-galactosidase (1:200) (DSHB), Rabbit anti-Wallenda (1:100, (5)) mouse anti-GFP (1:200, Roche, Basel, Switzerland), Rabbit anti-Ref(2)P (1:200 ...
-
bioRxiv - Biophysics 2022Quote: ... synthesized by IDT) with 66 mM digoxigenin N-hydroxysuccinimide ester (Roche) in 50 mM HEPES-NaOH ...
-
bioRxiv - Biochemistry 2020Quote: ... N-glycans were released with 2.5 U PNGase F (Roche, Australia) for 16 h at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.1% N-Lauroylsarcosine supplemented with 1mM PMSF and protease inhibitors (Roche)) at the concentration of 4×107 cells/ml ...
-
bioRxiv - Biophysics 2024Quote: ... Hen egg white lysozyme was purchased from Roche (Cat. N° 10837059001) or from Seikagaku Corp ...
-
bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
-
bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480; Roche Diagnostics, Mannheim, Germany) with SYBR Green I dye and gene-specific primers (Supplemental Table S8) ...
-
bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated with anti-DIG-POD antibody (Roche; 1:1000 in TNB; 12 hr, 4 °C), and washed in TNT (3 × 20 min ...
-
bioRxiv - Cell Biology 2024Quote: ... Sections were incubated overnight at 4°C with Anti-Digoxigenin-AP antibody (1:2000, Roche) in blocking solution ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated overnight at 4°C with anti-digoxigenin antibody (1:5000, Roche 11093274910). Following several washes to remove excess antibodies ...
-
bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM PMSF, Roche protease and phosphate inhibitor cocktails ...
-
bioRxiv - Cell Biology 2020Quote: ... then resuspended in 4°C homogenization buffer (0.6 M sorbitol, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM PMSF, 2× Roche cOmplete protease inhibitor cocktail ...
-
bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
-
bioRxiv - Cell Biology 2022Quote: ... and 5% glycerol with protease inhibitors (Roche, 1 tablet per 10 mL lysis buffer). Worm slurry was frozen in liquid nitrogen and stored at -80 °C until lysis ...
-
bioRxiv - Biochemistry 2020Quote: ... 1% NP-40 and 5% glycerol) supplemented with protease inhibitors (Roche Complete EDTA-free). Protein concentrations were determined by bicinchoninic acid assay (BCA protein assay kit ...
-
bioRxiv - Cell Biology 2023Quote: ... for 5 minutes and hiPSC-CMs were dissociated using 1:200 Liberase TH (Roche) in PBS for 20 min ...
-
Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging responsebioRxiv - Plant Biology 2023Quote: ... Each PCR reaction mix contained 5 μL of 2x SYBR green master 1 (Roche), 1 μL cDNA ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 mM EDTA) supplemented with 1 mM PMSF and protease inhibitor cocktail (Roche #11836170001). To ensure complete lysis ...
-
bioRxiv - Microbiology 2022Quote: ... 5 mM EDTA) supplemented with PMSF (1 mM) and Protease Inhibitor Cocktail (Roche #11836170001) followed by sonication of samples (25 % Amplitude for 10 seconds) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4°C against either HA (1:5000, Roche 12013819001), FLAG (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C in 1% goat serum in MABT with 1∶5000 alkaline phosphatase-coupled anti-Digoxigenin antibody (Roche Diagnostics). On the third day ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM DTT and 2 tablets of Complete Protease Inhibitor Cocktail Tablets (Roche) per 100 ml lysis buffer) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1% Penicillin/Streptomycin and 20 IU human IL-2 (Roche Diagnostics, Mannheim, Germany) for 5d ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 mg ml−1 iodoacetamide supplemented with Complete Protease Inhibitor Cocktail tablets (Roche) for 1 h at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 mg ml−1 iodoacetamide supplemented with cOmplete Protease Inhibitor Cocktail tablets (Roche) in Dounce homogenizer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 mM DTT supplemented with 1×cOmplete EDTA-free protease inhibitor cocktail (Roche), 1×PhosSTOP phosphatase inhibitor (Roche) ...
-
bioRxiv - Biophysics 2024Quote: ... 1 mM DTT and 2 mM MgCl2) supplemented with 1x protease inhibitor (Roche) and 1 µl benzonase (Merck) ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 µl Universal Nuclease Mix (Pierce™) and 1 tablet of cOmplete™ Protease Inhibitor Cocktail (Roche) were added and the cells were lysed via sonication ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 min) and plated onto 1.5 % agar TAP plate containing 10 µg mL-1 hygromycin B (Roche) and 20 µg mL-1 of paromomycin sulfate (Fisher BioReagents).
-
bioRxiv - Physiology 2023Quote: ... sections were incubated for 3 hours with Anti-Digoxigenin-AP Fab fragments (dilution 1:3000) (Roche, 11093274910) at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... All the signals were visualized by adding 3-3′-Diaaminobenzidinetetrahydrochloride (DAB substrate) solution (Roche) to the slides and counterstained with haematoxylin ...
-
bioRxiv - Cancer Biology 2020Quote: ... Nuclei were pelleted at 1350xg for 5 min at 4°C and lysed in 5 mL LB2 (10 mM Tris-Cl pH 8.0, 5 M, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 mM PMSF, Roche protease inhibitors) for 10 min at RT with rotation ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-SPD-5 (1:1000, a generous gift from B. Bowerman, Hamill et al., 2002) and anti-GFP (1:500, Roche) overnight at 4°C and with secondary antibodies Alexa488 (1:500 ...
-
bioRxiv - Biochemistry 2021Quote: Cell extracts were prepared in 1% NP40 lysis buffer (20 mM Hepes pH 7.5, 150 mM NaCl, 1% NP40, 50 mM NaF, 1 mM Na3VO4, 10% glycerol, and protease inhibitor cocktail from Roche) at 4 °C for 15 mins ...
-
bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 8, 450 mM NaCl, 1% CHAPS, 20 mM MgCl2, 5 mM ATP, 1 mM Dithiothreitol [DTT], 1X Roche Protease Inhibitor Cocktail ...
-
bioRxiv - Microbiology 2022Quote: ... The total qPCR reaction volume was 1.5 □μL with 0.5□ μl of DNA (40□ng□μl-1) and 1□μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5□μM of PCR primers ...
-
bioRxiv - Microbiology 2024Quote: ... The heat-denatured RNAs were separated on a 1% agarose gel (95% 1× MOPS buffer and 5% formaldehyde) and transferred to a positively-charged nylon membrane (Roche) by capillary flow ...
-
bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was performed in duplicate using 1:20 or 1:5 dilutions of the cDNA and SYBR Green I master mix (Roche) with a LightCycler 480 II (Roche ...
-
Ion channel function of polycystin-2/polycystin-1 heteromer revealed by structure-guided mutagenesisbioRxiv - Biophysics 2024Quote: ... Oocytes were subsequently transferred into 1 mL lysis buffer (Tris 25mM, NaCl 150 mM, EDTA 1mM, NP40 1%, Glycerol 5%, supplemented with Roche cOmplete™ protease inhibitor cocktail ...