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Citations for Roche :
5951 - 6000 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was assessed by WST-1 assay as per manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies ...
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bioRxiv - Genetics 2020Quote: ... 200 μl lysis buffer (8M Urea, 1% SDS, 50 mM Tris pH 8.5, Roche protease and phosphatase inhibitor) were added to the pellet and samples were vortexed and sonicated on ice ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM benzamidine) supplemented with 0.1 mg/mL lysozyme and one Complete EDTA-free protease inhibitor tablet (Roche) per 100 mL ...
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bioRxiv - Genomics 2021Quote: ... 250 nL of protease mix was added (1× NEB CutSmart buffer, 1.0 mg/mL Proteinase K (Roche, 000000003115836001)) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 mM imidazole) and incubated after addition of 1 or 10 mM phenylmethanesulfonyl fluoride (PMSF) (Roche, Basel, Switzerland), or 1 mM H2O2 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation in LNCaP IL1B overexpression and vector cells was assessed using Cell Proliferation Reagent WST-1 (Roche), per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10 ml of DMEM media and 1 mg/ml collagenase D (Sigma-Aldrich, COLLD-RO Roche, #11088866001). The tubes were placed on a gentleMACS Dissociator (MACS Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was carried out using LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany) following the manufacturer’s temperature program with 62°C as the primer hybridization and elongation temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in lysis buffer (1.5M NaCl, 20 mM Phosphate buffer pH 7.5, 20 mM Imidazole, 10mg/mL lysozyme, 1 tablet of Roche EDTA-free protease inhibitor cocktail Millipore Sigma 11873580001 ...
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bioRxiv - Immunology 2021Quote: ... minced thymuses from fetuses and postnatal mice were digested with 0.5 and 1 unit/ml Liberase TM (Roche) in the presence of 0.01% DNase I (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... wild-type or transgenic animals in an equal volume of Pedro’s buffer (30mM HEPES, pH 7.5, 100mM KOac, 10mM EDTA, 10% glycerol, 1 mM DTT, 1x Roche complete mini protease inhibitors ...
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bioRxiv - Immunology 2021Quote: ... and digested for 20 min at 37 °C in medium supplemented with 1 mg/mL Collagenase D (Roche) and 2000 U/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10000; Roche Diagnostics, Germany) conjugated with alkaline phosphatase ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100) complemented with final concentration of 1X EDTA-free Complete protease inhibitor cocktail (34044100, Roche) and 1 mM PMSF (78830 ...
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bioRxiv - Immunology 2023Quote: ... and the surface was immersed in 200 µl of ice-cooled gut buffer [1× protease inhibitor cocktail (Roche), 0.2 mg/mL trypsin inhibitor from soybean (Thermo fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then resuspended in sonication buffer (50 mM Tris-HCl pH 8.1; 10 mm EDTA; 1% Triton-X; 0,1% deoxycholate sodium; 100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) and sonicated 6 times with 2 min cycles (Branson Sonifier 250 ...
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bioRxiv - Developmental Biology 2023Quote: ... Riboprobes produced as described above were detected using alkaline-phosphatase-conjugated anti-digoxigenin Fab fragments (1:2000, [Roche]). Colorimetric signals were generated using a development solution containing 5-Bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Pathology 2023Quote: ... The slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Cat. # 950-500, Roche Diagnostics) for antigen retrieval ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were incubated with horseradish peroxidase (HRP)-conjugated anti-Dig (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were cut and immunodetected with either Horse Radish Peroxidase (HRP)-conjugated 3F10 anti-HA (1:2000, Roche) or HRP-conjugated Flag M2 (1:2000 ...
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bioRxiv - Immunology 2023Quote: ... Lungs were perfused with sterile PBS and digested for 1 hour with 625µg/mL collagenase D (Roche 11088875103) and 75U/mL DNase I (Sigma D4527) ...
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bioRxiv - Neuroscience 2023Quote: Tissues were diluted in RIPA buffer (50 mM Tris pH 8.0, 150 mM NaCl, 0.5% sodium deoxycholate, 0.1% SDS, 1% Triton-X100, freshly added protease/phosphatase inhibitors [Roche]), and homogenised using stainless steel beads and a QIAGEN TissueLyser II (shaking 1 min ...
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bioRxiv - Plant Biology 2023Quote: Total proteins were extracted from 6 g of frozen ground Arabidopsis seedling tissue with IP buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, 1% Triton, EDTA-free protease inhibitor cocktail [Roche]). Lysates were cleared by centrifugation (6,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation was performed at 4 °C for 1 h with pre-conjugated anti-HA affinity matrix (Roche 11815016001) or Anti-GFP Agarose (RQ2 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with DNAse I (4 U/ul) and 1 cOmpleteTM mini EDTA-free protease inhibitor cocktail tablet (Roche). The sample was lysed by French press at 17 KPsi (Constant Systems Ltd) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100) supplemented with 1X complete protease inhibitor cocktail and 1X complete phosphatase inhibitor cocktail (Roche) using a Precellys Evolution homogenizer (Bertin Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were washed thoroughly with digitonin buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Plant Biology 2023Quote: ... cells were ruptured by bead beating in lysis buffer (50 mM Tris-pH 8.0, 1 mM DTT, 0.5 mM PMSF and 2x PhosStop EASYpack-Roche) for 7 cycles with 30 seconds of beating at 400 rpm and 45 seconds on ice for each cycle ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study and their respective dilutions were: mouse monoclonal anti-GFP at 1/1000 (clones 7.1 and 13.1, Roche), mouse monoclonal anti-TY at 1/200 (53) ...
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bioRxiv - Molecular Biology 2023Quote: ... the mixture was resuspended in buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were washed thoroughly with digitonin buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1× Roche cOmplete protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed three times (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche complete protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... 5-7 washing steps with wash buffer (25 mM Hepes pH 7.4; 150mM NaCl; 0.5mM EDTA pH 8.0; 1 mM DTT and ½ tablet of proteinase inhibitor cocktail, Roche 11836170001) were performed before the beads were either sent for mass spectrometry or prepared with the appropriate amount of SDS for SDS/PAGE and Western blot ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were washed with 0.1% PBST and incubated with anti-Fluorescein-POD antibodies (1:500; Roche, #11426346910) in 1% blocking buffer overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study and their respective dilutions were mouse monoclonal anti-GFP at 1/1000 (clones 7.1 and 13.1, Roche), mouse monoclonal anti-TY at 1/250 (53) ...
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bioRxiv - Biochemistry 2023Quote: ... 30% glycerol [w/vol] and bromophenol blue) and 1 µl of proteinase K (14–22 mg/ml, Roche) and incubating at 37 °C for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... at 95°C for 60 minutes or with a medium concentration of protease (Protease 1; Roche Tissue Diagnostics) for 4 minutes ...
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bioRxiv - Microbiology 2022Quote: Cells were washed once with 1xPBS before harvesting in NP-40 buffer with protease inhibitor (200 mM NaCl, 50 mM Tris pH 7.4, 0.5% NP-40 Alternative, 1 mM dithiothreitol, and Roche Complete Mini ...
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bioRxiv - Molecular Biology 2022Quote: ... Asokoro Abuja using the COBAS AmpliPrep/COBAS TaqMan HIV type 1 (HIV1) v2.0 test (Roche Diagnostics, Basel, Switzerland). Whole genome sequencing of 61 blood samples was performed according to the Bonsall et al protocol10 ...
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bioRxiv - Cell Biology 2022Quote: ... the chromatin pellet was suspended with buffer B (20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 10 mM EDTA, and 1× complete protease inhibitor cocktail; Roche), and then mononucleosome was extracted ...
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bioRxiv - Cancer Biology 2022Quote: ... Library preparation was performed with 1 μg of DNA and KAPA HTP Library Preparation Kit (KAPA Biosystems, #KK8234). Resulting reads (50 base single-end reads ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Triton X-100 (0.1% ...
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bioRxiv - Molecular Biology 2024Quote: ... 400 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, 5% glycerol, 1x protease/phosphatase inhibitor cocktails, Roche). Nuclear lysates were diluted with 2 volumes of dilution buffer (20 mM HEPES pH7.9 ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM PMSF) and supplemented with a protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail Tablets, EASYpack, Roche). Cells were lysed by sonication on ice (Branson Digital Sonifier 450 ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell extracts were prepared by lysing cells in RIPA buffer (50 mM Tris HCl pH 7.4/150 mM NaCl/0.5% sodium deoxycholate/0.1% sodium dodecyl sulfate/1% NP-40) containing cOmplete protease inhibitor cocktail (Roche), followed by quantification using Bradford assays ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated by lysing cells (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.67% SDS, and 125 μg/ml proteinase K from Roche), followed by incubation 4 hours at 55°C with weak agitation ...
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bioRxiv - Neuroscience 2024Quote: ... Detection of DIG-labeled probe was performed with a fluorescein-conjugated sheep anti-DIG antibody (1:250, Roche). All buffers were made with RNase-free PBS or water.
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in buffer A complete (buffer A with 0.15% NP40, 0.5 mM DTT, 1 x Complete protease inhibitors, Roche), and dounced 40 times ...
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bioRxiv - Molecular Biology 2024Quote: Site directed mutagenesis of plasmids listed in Table 1 was carried out using KAPA HiFi HotStart premix (Roche) or Tks Gflex DNA polymerase (Takara Bio ...