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Citations for Roche :
5801 - 5850 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... For single cell preparation samples were incubated for 60-min at 37°C with gentle shaking in RPMI media supplemented with 50 mg/ml of Liberase TL (Roche #5401020001), 40 U/ml of DNAse I (Sigma-Aldrich #D4527) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
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bioRxiv - Microbiology 2023Quote: ... mycelia were resuspended in lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg/mL lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany)) and incubated at 37°C for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... The S100 extract was eluted from DEAE resin with 5 ml of S100 Elution Buffer (250 mM KHPO4 (pH 6.5),1x Mini protease inhibitor cocktail tablet (Roche, Basel, Switzerland)) and aliquoted and flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in 20 ml of S100 Lysis Buffer (10 mM KHPO4 pH 7.5, 1x Mini protease inhibitor cocktail tablet (Roche, Basel, Switzerland)) for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... and weighed on a fine balance to put 0.6g of skin per 3.5ml of digestion medium (0.385mg/ml Liberase TL-Roche 246749-in HBSS/PBS). Samples were incubated at 37°C for 40-60 minutes while shaking ...
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bioRxiv - Immunology 2023Quote: ... Uteri were isolated and carefully dissected to remove the yolk sac containing the fetus followed by mechanical disruption and digestion with DNase I 200 units/mL (Roche 10104159001) and Liberase DL 0.3 W units/mL (Roche 05466202001 ...
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bioRxiv - Cell Biology 2023Quote: Between 20,000 and 40,000 cells were seeded the day before the imaging experiment on 8-well ibidi dishes coated with 25 μg/ml laminin (Roche, 11243217001). When indicated ...
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bioRxiv - Plant Biology 2024Quote: ... The transformation mixture was plated on TAP medium containing 18 μg/ml Hygromycin B (Roche 10 843 555 001; Mannheim, Germany) using 0.9% agar to screen for colony morphology ...
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bioRxiv - Developmental Biology 2024Quote: Immune cell FACS was performed from ear skin from which cells were isolated using DNAse I 40 µg/ml (Roche, 11284932001) and 20 U/ml Collagenase Type I Worthington (LS0004194 ...
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bioRxiv - Genetics 2024Quote: ... Both mouse and human brain tissue were minced with a scalpel then homogenized in 0.5 mls of nuclei lysis buffer with RNAse inhibitor (Roche, Indianapolis, ID) then transferred to a larger tube with an additional 1.0 ml of nuclei lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... AF488-GAF-FL or AF546-GAF-ΔPOZ was flown through the protein channel diluted to 80 nM in imaging buffer (50 mM NaCl, 50g/mL BSA (Roche 10711454001), 0.05% NP40 (Sigma I8896) ...
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bioRxiv - Immunology 2024Quote: ... were resuspended in mHBSS at a density of 107 cells/ml and pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM in a BSA-coated centrifugation tube ...
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bioRxiv - Biochemistry 2024Quote: Bacterial cell pellets were resuspended in 50 ml of lysis buffer (50 mM Tris-HCl pH 8, 1 mM DTT, 5% glycerol, protease inhibitor cocktail [Roche 1183615300]). Cells in pellets were lysed by sonication on ice with 10 sec pulses of maximum setting followed by 1 min cooling period ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM phenylmethyl sulphonyl fluoride (PMSF)) plus EDTA-free protease inhibitor tablets (1 tablet per 50 mL, Roche, cat. no. 05056489001), 0.5% Triton-X100 ...
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bioRxiv - Immunology 2024Quote: ... sciatic nerves and brains were crushed with the back of a 3ml syringe in a serrated 24 well plate and triturated in digestion media (Liberase TM (0.208 WU/ml) (Roche Cat # 054010200001) and DNase I (40 ug/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Beads with cells were washed twice in 1 ml Digitonin Buffer (20 mM HEPES-KOH pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1x Roche cOmplete™; 0.05% digitonin), and resuspended in 50 µl Digitonin Buffer with addition of 2.5 µl CUTANA pAG-MNase (20x stock) ...
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bioRxiv - Microbiology 2020Quote: ... the strain of interest was inoculated from an exponential phase culture into 15ml of 7H9-OADC supplemented with kanamycin (Roche, 20ug/ml) and ATc (Sigma ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplification of the ND2 gene used 25 ml reaction volumes and included 1X Kapa HiFi HotStart ReadyMix (2X) (Kapa Biosystems, Wilmington, MA), 0.2 mM forward primer ...
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bioRxiv - Developmental Biology 2020Quote: ... Fixed embryos were homogenized in 2 ml cell lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.3% NP-40, 1x Roche Complete protease inhibitors cocktail) with a Dounce Homogenizer on ice and centrifuged 5 min 2,300g at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tissues were homogenized in 1 ml N-PER (brain) or T-PER (other organs) + Complete Mini protease inhibitor cocktail tablet (Roche, Mannheim, Germany) in FastPrep Lysing Matrix D tubes on a FastPrep homogenizer ...
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bioRxiv - Molecular Biology 2021Quote: ... Putative hygromycin and neomycin-resistant candidates transformants were screened on TB3 media supplemented with 250 μg/mL hygromycin B (Roche Applied Science) and 200 μg/mL G418 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Screening of hygromycin- and neomycin-resistant transformants was performed on TB3 media supplemented with 250 μg/mL hygromycin B (Roche Applied Science) and 200 μg/mL G418 (Invitrogen) ...
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bioRxiv - Immunology 2019Quote: ... samples were boiled for 10 min in 1 mL of TE buffer and were loaded on a nylon membrane (Roche, Basel, Switzerland) using a Minislot 30 apparatus (Immunetics ...
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bioRxiv - Developmental Biology 2019Quote: ... Single cell suspension of testicular cells was obtained by treating seminiferous tubules in 0.5 mg/ml Collagenase A (Roche, Catalog No: 23324223) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Tongues were removed and injected beneath the lingual epithelium with 0.2 mL enzyme solution (3 mg Dispase II, 0.7 mg collagenase B (Roche diagnostics, Indianapolis, IN, USA) and 1 mg Trypsin Inhibitor in 1mL Tyrode’s) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were dehydrated and rehydrated in ethanol and water and incubated with 10 μM dNTP mix containing Digoxigenin-dUTP and 20 U/mL Klenow Fragment (Roche; Basel, Switzerland) at room temperature for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse hind limb muscles (tibialis anterior, quadriceps and gastrocnemius) were minced and incubated in a 1 mg/ml collagenase/dispase solution (Roche, Basel, Switzerland) with agitation at 37°C for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... from 18-day old mouse pups were disrupted using forceps and a razor blade in 1 mL of 1× PBS with protease inhibitors (05896988001, Roche Complete Ultra). The cell suspension was then gently added to a 15-mL conical tube with 5 mL of 1× PBS with protease inhibitors and allowed to settle ...
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bioRxiv - Molecular Biology 2022Quote: Individual murine left atrial cardiac myocytes were isolated by perfusion with a Tyrode’s enzyme solution containing 20 μg/mL Liberase™ (Roche, Indianapolis, IN) or a collagenase+protease mix ...
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bioRxiv - Biophysics 2022Quote: ... and mixed with 1 mL Ni Sepharose 6Fast Flow (Cytiva, Tokyo Japan) (MinD) or cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland) (MinE and msfGFP-MinC) ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse MEC organoids were prepared by manual chopping of mouse mammary gland tissues harvested from 10–12-week-old mice followed by digestion with shaking for 1 hr at 37 °C with Collagenase A from Clostridium histolyticum (10 mg/mL; Roche, Cat. #: 13560925) and Hyaluronidase from bovine testes (1 mg/mL ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Microbiology 2020Quote: ... containing Roche mini-complete protease inhibitor EDTA-free and Roche PhosSTOP (1 tablet of each per 10 mL of buffer) (Roche, Basel, Switzerland). Samples (on ice ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then harvested using 1 ml of ice-cold 1X PBS supplemented with 0.01% Phosphatase inhibitor (PhosSTOP, Roche, Cat. no. 04906845001). Pellets corresponding to 10 plates of a 10 cm plate were pooled together and centrifuged at 5,000 rpm for 5 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: Bacteria-captured beads were incubated at 37°C for 1 hour with 10 mg/mL of lysozyme (Cat # 10837059001, Roche, Basel, Switzerland) diluted in 10 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Physiology 2019Quote: ... Powdered tissue was then scraped into a Dounce tissue homogenizer and 1.2 mL of ice-cold 1X PBS supplemented with EDTA-free cOmplete protease inhibitor cocktail (Roche, Indianapolis, IN, USA) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... The Dynabeads™ were washed once with 2 ml of PBS and coated with 15 μg of mouse monoclonal anti-GFP antibody (Roche Applied Science). The beads were finally washed three times with 2 ml of lysis buffer and added to the whole cell extract for a 2 h incubation with gentle rotation ...
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bioRxiv - Microbiology 2020Quote: ... Treatment of organs was as followed: PBS supplemented with protease inhibitor (1 tablet cOmplete protease inhibitor, Roche in 35 mL of PBS) was added to the frozen organs (volume depending on the weight of the organ ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and transferred in an enzyme solution (2 mg/mL Collagenase/Dispase (Roche, Bale, Switzerland), 0,147 µg/mL TLCK (Lonza ...
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bioRxiv - Immunology 2020Quote: ... cut into small pieces and digested for another 1 h at 37°C in 1 mg/ml Collagenase D (Roche, Basel, Switzerland). To obtain single-cell suspensions ...
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bioRxiv - Developmental Biology 2019Quote: ... tissue pieces were rinsed in ice cold PBS and incubated in a combination of 200 μg/ml Liberase (Liberase™ TL Research Grade, Roche, 05401020001) and 0.1 mg/mL DNaseI in DMEM/F12 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Ganglia were mechanically disrupted with a pipette tip until obtaining a single cell suspension in culture media supplemented with DNAse I grade II at final concentration 3mg/ml (Roche, Cat# 104159), which was centrifuged at 1300 rpm (302 rcf ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were treated with the oligonucleotide mixed with DOTAP liposomal transfection agent (35 μg/ml) (#11811177001, Roche Diagnostics GmbH, Mannheim, Germany) for 3 to 6 hrs (16) ...
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bioRxiv - Biochemistry 2021Quote: The pupae infected by the baculovirus were homogenized with a buffer solution (20 mM Tris-HCl, 150 mM NaCl, 5 mM DTT, 1mg/ml phenylthiourea, one inhibitor cocktail tablet (EASYpack, Roche, Basel, Switterland) per 50 ml solution ...
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bioRxiv - Developmental Biology 2020Quote: The testes from ten to twelve 19dpc embryos form 9 different mothers were collected and dissociated using collagenase/dispase (Roche, 1mg/ml), at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor tissues were minced into 1×1 mm fragments and enzymatically dissociated in HBSS containing 1 mg/ml collagenase A (#11088793001; Roche, Basel, Switzerland) and 1 μg/ml DNase I (#07900 ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... The cells were lysed using RIPA buffer (Tris-HCl 10 mM, 1% SDS, 10 mg/ml deoxycholate, 150 mM NaCl, 1% NP40 and protease inhibitors cocktail [Roche, Mannheim, Germany]). The total protein samples were separated using by 12% SDS-PAGE and transferred onto nitrocellulose membranes (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... TES (Kieser et al. 2000) was used for transformation and all fusion experiments supplemented with 1 mg/mL DnaseI (Roche Diangnostics GmbH). Antibiotics apramycin (Duchefa Biochemie ...
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bioRxiv - Immunology 2021Quote: ... an equimolar amount of DMSO was used to stimulate the cells in triplicate wells and as positive controls phytohemagglutinin (PHA, Roche, 1μg/ml) and a cytomegalovirus MP (CMV ...