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Citations for Roche :
5701 - 5750 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Seed histological sections were washed with Phosphate Buffered Saline solution (PBS) and digested with 1 mg/ml proteinase-K (Hoffmann-La Roche). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Stable cell lines were established by selection with Schneider’s Drosophila medium supplemented with 300µg/ml hygromycin (Roche, Palo Alto, CA).
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bioRxiv - Molecular Biology 2024Quote: ... samples were washed three times with 10 ml of ice-cold PBS supplemented with Complete Protease Inhibitor (Roche, Basel, Switzerland), and the cells were resuspended in 1 ml of the same buffer ...
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bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and lysed in 50 mL McIlvaine buffer containing EDTA-free cOmplete Mini Protease Inhibitors (Roche). Cells were shaken at 4°C and 450 rpm for 10 min followed by one hour of incubation at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... life-Tein LLC) was mixed with 50µl of PBS containing 1% Tween20 and 5ng/mL anti-HA-Peroxidase 3F10 antibody (Roche #12013819001) and transferred to the wells ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was resuspended (20 mL/L culture volume) in lysis buffer (20 mM Tris pH 8, 150 mM NaCl, and protease inhibitor cocktail, Roche). These resuspended cells were disrupted using sonication (QSonica sonicator ...
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bioRxiv - Bioengineering 2024Quote: ... or 1 M NaCl (for ori designs), 20 µg/mL DNase (#A3778, ITW Reagents) and protease inhibitor cocktail (04693132001, Roche)) ...
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bioRxiv - Plant Biology 2024Quote: ... incubated three days at room temperature in the dark and then moved to selection media (CIM + Hygromycin (40 mg/ml final concentration, Roche) + Timentin (200 mg/ml final concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Cell Biology 2019Quote: ... pH 7.5) containing protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each 1 tablet per 15 mL, Roche, Basel, Switzerland), and diluted in 6×SDS-PAGE sample loading buffer (Beyotime ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions of tumors were generated by dicing tumors with a razor blade followed by enzymatic digestion for 30 minutes at 37C in medium containing 1 mg/mL Collagenase D (Roche Diagnostics) and 1μg/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The wells containing fixed cells were washed once with 1X PBS and stained with DAPI (50 μg/ml) for (Roche, 10236276001) 10 min at RT and protected from light ...
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bioRxiv - Immunology 2021Quote: ... was added to the PBMCs for 5 minutes before transferring drop-wise to a 10ml volume of warmed media (RPMI/20%FBS) containing 200U/ml of DNase I (Roche, Switzerland). After 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... For the neuraminidase treatment each well was supplied with 500 μL of a solution containing 50 mU/mL neuraminidase (Roche Diagnostics) in cold PBS+++ pH adjusted to 5.5 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 % Tween-20 and 1 mM β-mercaptoethanol supplemented with 1 mg/mL lysozyme and protease inhibitor cocktail tablet (Roche, Switzerland) at 4°C ...
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bioRxiv - Zoology 2019Quote: ... TBST washes of 15 minute each at room temperature were performed thrice and subsequently the tissue was stained using NBT (working concentration 500ug/ml; Roche #11383213001) and BCIP (working concentration 562.5ug/ml ...
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bioRxiv - Genomics 2020Quote: ... tissue was cut into 1mm x 1mm cubes and placed in 0.25mg/ml liberase TH dissociation medium (Roche Diagnostics, Indianapolis, USA). Following further dissection ...
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bioRxiv - Immunology 2021Quote: ... the lamina propria was dissected into small pieces with scissors and incubated in digestion buffer (RPMI 1640 containing 0.17 mg/mL Liberase TL (Roche, Basel, Switzerland) and 30 µg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 1 mM DTT and incubated at RT in 0.05 mg/mL sequencing grade trypsin (Roche Applied Science). Digestions were stopped with 2X Laemmli sample buffer and placed on ice until analysis ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Neuroscience 2020Quote: ... 5 ml of lysis buffer (10 mM Tris, 10 mM NaCl, 3 mM MgCl2, 0.1% Nonidet P40 substitute (Roche/ Sigma, 11754599001), 0.2 U μl−1 RNase inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... synovial tissues from three patients with RA were minced and incubated with 1 mg/mL collagenase/dispase (Roche, Indianapolis, IN, USA) in phosphate-buffered saline (pH7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 30 min in Ringer solution before plating on fibronectin-coated glass coverslips (human plasma fibronectin at 10 µg/ml, Roche 10838039001).
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was transfected into Neuro2a cells and clonal lines selected by incubation with Hygromycin (300 mg/ml, Roche (Nutley, NJ)) ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were re-suspended in 1 mL lysis buffer (5 mM EDTA, 1% NP-40 in PBS) supplemented with 2X cOmplete™ inhibitor (Roche) and were sonicated until the lysate became turbid ...
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bioRxiv - Genomics 2022Quote: ... were washed twice with PBS/BSA (5 mg/ml BSA) and incubated with 3 μl of anti-Myc (Roche, cat # 11667203001) or anti-Rpb3 (Neoclone ...
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bioRxiv - Cancer Biology 2022Quote: ... AML blast cells were incubated in 1 ml of ice-cold 0.1 M Na2CO3 containing complete protease inhibitor (Roche, Penzberg, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris-HCl (pH 8.0) and protease inhibitor (one tablet of Roche cOmplete™ mini per 10 ml lysis buffer) [22] ...
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bioRxiv - Microbiology 2021Quote: The following agents have been used in the study: cotrimoxazole (Bactrim, sulfamethoxazole and trimethoprim 150/ 15 mg/ mL; Roche, Basel, Switzerland), artesunate (10 mg/ mL ...
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bioRxiv - Microbiology 2020Quote: ... The pellet of 100 mL was resuspended in 1 mL cold fractionation buffer (40 mM HEPES, 0.1 mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Roche Sigma)) and the pellet of 1 mL lysed with Bug Buster (pellet sample ...
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bioRxiv - Neuroscience 2021Quote: ... Briefly, ganglia from adult Aplysia were treated with Dispase® II (10 units/ml) (neutral protease, grade II) (Roche, Indianapolis, IL) at 35°C for ∼3 h and then desheathed ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested either for 1 hour at 37°C in a digestion mix of 3 mg/ml collagenase type A (Roche, 11088793001) and 25 μg/ml DNAse (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... The material was collected in a 50mL centrifuge tube and supplemented with 0.01 mg/mL Liberase TL (Roche, Woerden, the Netherlands); the suspended tumor tissue was incubated at 37°C for 3-5 hours while shaking at 250 rpm ...
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bioRxiv - Cancer Biology 2021Quote: HS and healthy skin tissues were cut into small pieces and were digested with 2.5U/ml Dispase-II (Roche, Cat#04942078001) in Hanks’ Balanced Salt Solution (HBSS ...
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bioRxiv - Immunology 2021Quote: ... We resuspended the glomerular fraction in 1mL HBSS buffer with Ca2+ and Mg2+ (Hyclone, Logan, UT) containing 0.1-0.2 mg/mL Liberase™Research Grade (Roche, Mannheim, Germany), and incubated glomeruli in 24-well tissue culture plates at 37°C for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by perfusion with Hanks’ balanced salt solution with Mg2+ and Ca2+ containing 0.1 mg/mL of Liberase (Roche Applied Science). Following the removal of the liver ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were subsequently incubated in 1 ml of blocking solution (1:1 (v/v) of Western Blocking Reagent (Roche, Cat#11921681001) and PBT ...
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bioRxiv - Neuroscience 2019Quote: ... DRGs were dissected out from 8-week old adult mice and incubated with collagenase A (1 mg/ml; Roche, Basel, Switzerland) for 90 minutes and then with 1X TrypLE (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... HCXII-0155) was incubated with neutrophils that were pre-treated with vehicle or DNase I (10U/ml, Dornase alpha, Roche, Germany) for 1h ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were lysed 72 hours post transfection or post (first) transduction using the DirectPCR lysis reagent (PeqLab) supplemented with 200 μg/ml proteinase K (Roche Diagnostics). The targeted genomic locus was then PCR amplified with primers flanking the expected cutting site (Supplementary Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to a protease protection assay by resuspension in 250 μl of freshly prepared 1 mg/mL Pronase (Roche, 10165921001)/0.01% Saponin/PBS at 12°C for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 seconds each with intermittent cooling on ice ...
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bioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation and resuspended in 20 mL of lysis buffer (20 mM Tris-HCl, 500 mM NaCl [pH8], containing a protease inhibitor cocktail (Complete®, Roche)) ...
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bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Genetics 2020Quote: Cells grown in 5ml volume to desired optical density were suspended in 500μl cell lysis buffer (150mM NaCl, 0.5mM EDTA, 0.5% NP40, 1mM PMSF, 0.5mg/ml Lysozyme, cOmplete protease inhibitor[Roche], 10mM Tris pH 7.5).The suspension is kept on ice for 15-30 minutes on ice and then sonicated ...
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bioRxiv - Physiology 2021Quote: ... containing cOmplete EDTA-free protease inhibitor cocktail at a concentration of 1 tablet/7 ml (Roche Applied Science, Indianapolis, IN, USA)) by repeated passage through a 20-gauge needle to obtain plasma membrane-enriched preparations ...