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Citations for Roche :
5501 - 5550 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... Explanted subpleural peripheral lung tissue was digested to single-cell suspensions using Liberase DL (Roche) and bovine pancreas DNAse I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... After 72h cells were lysed using ice cold RIPA buffer with protease/phosphatase inhibitor (Roche) for 30 min and centrifuged for 15,000 xg for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells/collagen discs were digested in HBSS adding 1:50 10mg/ml Collagenase P (Roche) for ∼30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in 1X RIPA Buffer (CST, 9806) supplemented with protease inhibitors (Roche, 11836153001) and then centrifuged at max speed for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Cancer Biology 2021Quote: ... Library qualities and sizes were checked with the TapeStation 4200 and then quantified using the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems). Libraries were sequenced on the Illumina NextSeq500 sequencer to a depth of about 50 millions of 75-bp pair reads per library.
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bioRxiv - Cancer Biology 2021Quote: ... RT-PCR was performed using first strand synthesis with specific primers (Reagents) and SYBR FAST qPCR Kit (Kapa Biosystems, Wilmington, MA) in a StepOne cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Microbiology 2020Quote: ... The fragments were treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA Illumina Library prep kit (KAPA biosystems). Libraries for the rest of the samples were prepared in 96-well plates ...
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bioRxiv - Developmental Biology 2021Quote: ... Illumina-compatible sequencing libraries were prepared by using a modified version of the KAPA HyperPrep library kit (KAPA BioSystems, Willmington, MA). Quality check steps were performed to assess the fraction of proximally ligated DNA labeled with biotin ...
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bioRxiv - Cell Biology 2020Quote: ... Each RNA-seq library was analyzed by the fragment analyzer (Advanced Analytical Technologies) and quantified by KAPA Library Quantification Kit (KAPA Biosystems) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR reactions were performed with fast Start Universal Probe Master kit and UPL-probe system in a LightCycler 480 Instrument (Roche Diagnostics) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... with on-column DNase digestion followed by cDNA synthesis using the Transcriptor First Strand cDNA synthesis kit (Roche Molecular Diagnostics, 04379012001) using random primers ...
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bioRxiv - Immunology 2021Quote: Approximately 200-500 ng of FFPE RNA extracted from FFPE slides with a DV200 range between 3-99 or 65-100 ng of fresh frozen RNA (DV200 98-99) per sample were used for RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). The number of pre-capture PCR cycles was adjusted based on the quality and quantity of RNA extracted from the samples ...
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bioRxiv - Neuroscience 2020Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche-KK4824). Individual libraries were normalized to 1.30 nM in preparation for Illumina sequence analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The library size distribution was checked on an Agilent high-sensitivity DNA chip and the library quantified using the KAPA library quantification kit (KAPA Biosystems). For each reaction ...
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bioRxiv - Genetics 2021Quote: ... PCR products were cleaned using Ampure XP Beads (Agencourt) and quantified using the KAPA Library Quantification Kit for Illumina® Platforms (Roche). Libraries were sequenced on a NextSeq sequencer (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples with RIN >7 were used for paired-end sequence library construction using the KAPA mRNA HyperPrep Kit (Roche # 08098123702). For ChIP samples ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was enriched by poly-A capture and mRNA-seq libraries were generated using KAPA Stranded mRNA-seq Kit (KAPA Biosystems). Libraries were generated from two biological replicates ...
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products from each level were pooled in equimolar concentration and the pool was converted into a sequence-ready library with the Kapa Hyper Library Construction kit (Roche, CA) with no PCR amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... The entire ChIP DNA or 10 ng DNA in the case of ChIP input was used to make DNA library with the KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
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bioRxiv - Genomics 2020Quote: ... The libraries were visualized with the Fragment Analyzer (Advanced Analytical Technologies) and quantified using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems). [3] Based on the concentration ...
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bioRxiv - Immunology 2021Quote: ... and used as a template for the synthesis of cDNA utilizing the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). Total genomic DNA was extracted by boiling BMDCs in a 50 mM NaOH solution as previously described (47) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Immunology 2020Quote: ... Tagmented DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and then purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the Ovation RNA-seq System V2 (NuGEN Technologies) and Hyper Prep Kit (Kapa Biosystems). Libraries were checked for quality and concentration using the D5000 ScreenTape assay (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: ... 1μg of total RNAs were used to prepared ribo-minus RNA-seq libraries using the KAPA Stranded RNA-Seq Kit with RiboErase (Roche, KK8484), and the depletion of rRNAs confirmed by fluorometric quantification using the Qubit RNA HS Assay kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... target sites were amplified directly from genomic DNA in the lysate using a KAPA HiFi HotStart PCR kit (KAPA Biosystems, KK2501) as previously described18 ...
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bioRxiv - Genomics 2021Quote: ... 1-8 ng of ChIPed DNA was treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA-Illumina library creation kit (KAPA biosystems). The ligated products were enriched with 8-10 cycles of PCR (HiFi premix ...
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bioRxiv - Genomics 2021Quote: Genomic DNA libraries were constructed for high throughput sequencing using the KAPA hyper Prep Library Preparation Kit (Kapa Biosystems, Woburn, MA). Mechanical DNA shearing was performed with the Covaris S220 through microTube-50 AFA Fiber Screw-Cap (Covaris® ...
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bioRxiv - Developmental Biology 2020Quote: ... Library preparation for ChIP-sequencing (ChIP-seq) was performed as described 66 using the KAPA LTP Library Preparation Kit (KAPA Biosystems) and 2 ng of input DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... All PCR reactions were performed using SYBR-containing master mix from the KAPA Real-Time Library Amplification Kit (Kapa Biosystems #KK2702) and terminated in the mid-exponential phase to limit over-amplification ...
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bioRxiv - Immunology 2021Quote: ... Tagmented DNA was ligated to a unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (Roche, #07958927001), and then purified using AmPure Beads XP (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... the DNA was ligated to i5/i7 barcoded primers using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems). Next the DNA was purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of RNA for each sample were reverse transcribed using the Transcriptor first strand cDNA synthesis kit (Roche, Germany). For real-time quantitative PCR reactions ...
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Cell Ecosystem and Signaling Pathways of Primary and Metastatic Pediatric Posterior Fossa EpendymomabioRxiv - Cancer Biology 2020Quote: ... cDNA quality of the libraries was evaluated with a bioanalyzer and quantified using a KAPA Library Preparation Kit (Roche sequencing, KK4824). Libraries were sequenced using an Illumina NextSeq 500 on high output mode with 20 bp (read 1 ...
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bioRxiv - Immunology 2020Quote: ... For immunohistochemistry paraffin-embedded normal human skin and ES was stained for human Cytokeratin 5/6 according to the manufacturer’s protocol using a Ventana BenchMark Series automated slide stainer with ultraView Universal DAB Detection kit (Roche, 760-500).
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared by the Genome Technologies core service at The Jackson Laboratory using the KAPA RNA Hyper Prep Kit with RiboErase (HMR) (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The selected fragments were then end repaired, A-tailed and ligated to Illumina compatible adapters (IDT, Inc) using KAPA Illumina library creation kit (KAPA biosystems). Libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Bioengineering 2020Quote: ... The culture supernatant for each condition was harvested for RNA extraction using the HP Viral RNA Kit (Roche, Cat no. 11858882001) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... pGEM clones were linearized with NotI and transcribed with SP6 or T7 RNA polymerase using the DIG RNA labelling Kit (Roche 1277073) to generate antisense or sense probes ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting PCR products were purified and used as templates for in vitro transcription by employing DIG RNA Labeling Kit (SP6/T7) (Roche #11175025910). The resulting RNAs generated from SP6-mediated in vitro transcription were used as positive probes (cRNA ...
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bioRxiv - Molecular Biology 2021Quote: 200 μL of eluate was extracted on the Magna Pure 96 using a DNA and Viral NA Small Volume Kit (Roche, 06543588001) with the universal small volume protocol and eluted into 50 μL proprietary elution buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). Customized adapters with unique molecular indexes (UMI ...
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bioRxiv - Cancer Biology 2021Quote: LP-WGS libraries were prepared with 50 ng of sonicated tumor DNA (extracted from FFPE as described above) and patient-matched buffy coat DNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Illumina single index Tru-Seq adaptors (idtdna.com ...