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Citations for Roche :
5351 - 5400 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Extracted RNA samples underwent library preparation for polyA tailed mRNA with a RNA concentration of >1ng/1ul using with Kapa RNA hyperPrep kit (Roche), followed by 75 cycles of Next-generation sequencing with NextSeq® (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Illumina-compatible sequencing libraries were prepared by using a modified version of KAPA HyperPrep library kit (KAPA BioSystems, Willmington, MA). Quality check steps were performed to assess the fraction of proximally ligated DNA labeled with biotin ...
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bioRxiv - Molecular Biology 2024Quote: ... were subjected to library preparation after rRNA depletion and poly(A)+ RNA enrichment using the Kapa Hyper Stranded mRNA library kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Isolated mouse CD11b+ microglia were resuspended in DPBS and immediately mixed with lysis buffer of the High Pure RNA Isolation kit (#11828665001, Roche). RNA was extracted following the kit instructions and sent for sequencing to Novogene Europe (below).
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bioRxiv - Neuroscience 2024Quote: 1 µg of each total RNA sample was used to synthetize cDNA with Transcriptor First Strand cDNA synthesis Kit (Roche) according to the manufacturer’s instructions with the additional denaturation step at 65°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were ribo-depleted followed by library preparation with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, 8098140702). Next ...
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bioRxiv - Neuroscience 2024Quote: ... Gene expression was determined by quantitative rt-PCR using the FastStart Essential DNA Green Master kit (06402712001) in a LightCycler 480 II (Roche) and normalized to the mean of the 18s and HPRT expression ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were used to prepare NGS libraries with patient-specific adapter index sequences (HTP Library Preparation Kit, KAPA Biosystems). The enriched libraries were then sequenced on an Illumina MiSeq system ...
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bioRxiv - Genomics 2024Quote: ... 20-250 ng of genomic DNA was prepared for sequencing using the KAPA HyperPrep Kit with Library Amplification (Roche, KK8504). Target enrichment with a customized bait panel targeting 538 cancer-relevant genes (Table S4 ...
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bioRxiv - Biochemistry 2024Quote: ... The synergistic effect was determined based on the amount of acetic acid released using an acetic acid assay kit (Roche).
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). The qPCR was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the Kapa Hyper Prep library preparation reagents following the manufacturer’s protocol (Kapa Hyper Prep Kit, Roche 07962363001). ChIP libraries were sequenced by the McGill University Genome Centre using the Illumina HiSeq 4000 at 50 bp single reads or NovaSeq 6000 at 100 bp single reads.
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bioRxiv - Genomics 2024Quote: ... and subsequent buffer (Reagent W2, Buffer W3 and Enzyme W4) were replaced with KAPA HiFi HotStart Uracil+ Ready Mix Kit (Roche), which included an uracil tolerant polymerase ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of synthesized cDNA was estimated using Qubit ssDNA assay kit and STX11 TaqMan probes were used to perform the QPCR using LightCycler 96 (Roche). Beta-actin was used as housekeeping control.
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bioRxiv - Genomics 2024Quote: ... or the MagNA Pure 96 DNA and Viral NA Small Volume Kit automated on the Magna Pure 96 (Roche, Switzerland) (SSI and L&F) ...
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bioRxiv - Zoology 2024Quote: Virus nucleic acid was isolated using the Roche High Pure viral nucleic acid extraction kit (Roche Diagnostics GmbH, Manheim, Germany). The extraction process involved adding 200 μl of the working binding buffer (binding buffer mixed with poly[A] carrier RNA ...
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bioRxiv - Biophysics 2024Quote: ATP synthesis and hydrolysis activities of EhVoV1 reconstituted into liposome were measured using a luminometer (AB-2270, ATTO) and the luciferin/luciferase reagent (ATP bioluminescence assay kit CLS II, Roche). ATP-dependent light emission from luciferin catalyzed by luciferase was monitored (10 ...
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bioRxiv - Genetics 2024Quote: ... the origin of replication and the ampicillin resistance gene from pUC19 and the terminator from the pCAG mammalian expression vector were PCR amplified with KAPA HiFi HotStart ReadyMix PCR Kit (Roche). The lentiviral pLG1 library vector (Addgene #217306 ...
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bioRxiv - Cancer Biology 2024Quote: ... gene expression (5’Gex) and TCR libraries were generated then quantified via Agilent Technologies 4200 Tapestation and KAPA library quantification kit (Roche). Targeted read depth included 25,000 reads per cell for gene expression libraries and 5,000 reads per cell for TCR libraries ...
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bioRxiv - Biophysics 2024Quote: [Na+] and [K+] unintendedly contained in the Type II-S lipid and a luciferin/luciferase reagent (ATP bioluminescence assay kit CLS II, Roche) were estimated using inductively coupled plasma optical emission spectrometer (ICP-OES ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized using the qRT-PCR Kit (Toyobo, FSQ-101) and the quantitative reverse-transcriptase PCR (qRT-PCR) was conducted using FastStart SYBR Green Master (Roche) on a LightCycler qPCR system (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: The ability of CATCH-AdsA gels to dephosphorylate ATP was tested by measuring ATP concentration using the ATP Bioluminescence Assay Kit CLS II (Roche). Empty and 9uM CATCH-AdsA gels were prepared at 40uL in syringes as described previously ...
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bioRxiv - Bioengineering 2024Quote: ... Only RNA samples with an RNA Integrity Number (RIN) above 8.0 were selected for library construction using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche Diagnostics). The adapter ligation step of library preparation involved a 1:10 dilution of NEBNext® Multiplex Oligos for Illumina® Sets 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic libraries from approximately 500 ng gDNA were prepared using the standard KAPA paired-end sample preparation kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... genomic DNA from individual larvae or fin-clipped tail fins were extracted using the Kapa Express Extract kit (Kapa Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... and 7SL were amplified from RT-PCR of testicular RNA using the primers containing T7 and T3 promoter sequence (Supplemental Table S5) and used for cRNA probe synthesis with T3 RNA polymerase using DIG RNA labeling kit (Roche). Signals of 45S pre-rRNA ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and RNA-seq library preparation were constructed either using the KAPA stranded RNA-seq library preparation kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was performed by using using SYBR green kit (11201ES03, Yeasen) on a LightCycler 480 system (Roche, Penzberg, Germany). The primers used were as follows ...
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bioRxiv - Biochemistry 2024Quote: IL-2 plasmids were mutated by PCR amplification using primers containing the mutations of interest the KAPA HiFi HotStart ReadyMix PCR kit (Roche) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Final library quantification was performed using high-sensitivity Qubit assay and the KAPA Library Quantification Kit (Roche, cat. no. KK4824) with 1:10,000 and 1:20,000 dilutions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used a Covaris E220 sonicator to shear DNA and we then prepared genomic libraries using the KAPA HyperPlus kit (Roche). Individual libraries were indexed using KAPA UDI’s and pooled libraries were sent to Novogene for Illumina paired-end 150 bp sequencing on a Novaseq X.
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: Whole-cell extracts were prepared using RIPA buffer supplemented with CompleteTM protease inhibitors cocktail (Roche) and phosphatase inhibitors PhosStopTM (Roche ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were made in RIPA-buffer supplemented with Protease inhibitor cocktail (11836170001, Roche), 1mM NaF and 1mM NaVO3 ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in ice-cold RIPA buffer with cOmplete™ protease inhibitor (Roche) and/or phosphatase inhibitors (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: RCC cells were grown in serum-free medium supplemented with 0.1% bovine serum albumin (Roche) and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were collected in ice-cold PBS containing protease and phosphatase inhibitor cocktails (Roche). The cells were used to measure Rab7 activation using the Rab7 Activation Assay Kit (#NEBB40025 ...
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bioRxiv - Cell Biology 2020Quote: Cytoplasmic histone-complexed DNA fragments were determined in islets using the Cell Death ELISAplus (Roche), per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... cells were lysed in radioimmunoprecipitation assay buffer containing complete protease inhibitor cocktail (Roche, Basel, Switzerland) on ice and ultracentrifuged ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed in 2X PIC (Roche mini-tabs, 1 tab in 5 ml = 2X) and stored at -80°C until needed.
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bioRxiv - Cancer Biology 2022Quote: Total proteins of cells were extracted using RIPA lysis buffer (Beyotime) with Protease Inhibitor (Roche) and Phosphatase Inhibitor (Roche) ...
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bioRxiv - Genomics 2020Quote: ... Then WST-1 assay was performed by adding WST-1 reagent (cell proliferation reagent, Roche) directly to the culture wells ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with siRNA oligonucleotides using X-treme GENE 9 DNA Transfection reagents (Roche) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm, 1 min on, 2 min off) in the presence of 300 uL acid-washed glass beads (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was reconstituted in 10 mL PBS containing protease and phosphatase inhibitors (Roche) and centrifuged again at 1100 RPM for 4 minutes ...
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bioRxiv - Biophysics 2021Quote: ... The resuspended cells were incubated on ice with one Complete EDTA-free protease inhibitor (Roche) tablet as well as with 100 U of DNAse (ArcticZymes ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was reconstituted in 10 mL PBS containing protease and phosphatase inhibitors (Roche) and centrifuged again at 1100 RPM for 4 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the cells were resuspended in 600 μl of PBS containing protease inhibitors (Roche, Cat# 05892791001) and split into two 300 μl aliquots ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with constructs using the X-tremeGENE HP DNA transfection reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 1mM EDTA and supplemented with complete EDTA-free cocktail tablets (1 tablet/50ml cells; Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were plated on 25-mm diameter coverslips and transfected using Fugene6 transfection reagent (Roche) according to the manufacturer’s protocol ...