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Citations for Roche :
501 - 550 of 1448 citations for Recombinant Human CD19 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Digoxigenin-labeled sense and antisense RNA probes were synthesized following manufacturer’s instructions (Roche Applied Science). Mature green fruits (21 DAA ...
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bioRxiv - Genetics 2020Quote: ... Digoxigenin-labeled probes were generated from linear plasmid DNA using DIG RNA Labeling Mix (Roche) and hybridized probes were detected with anti-digoxigenin antibody (Anti-Digoxigenin-AP ...
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bioRxiv - Microbiology 2021Quote: ... cells were labeled beforehand for 30 minutes with an anti HA antibody (3F10, Roche 11867423001) diluted at 1:200 in PBA ...
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bioRxiv - Developmental Biology 2022Quote: ... digoxigenin-labeled probes were recognized using an anti-DIG antibody coupled to peroxidase (11207733910, Roche), and fluorescein-labeled probes were recognized using an anti-FLUO antibody coupled to peroxidase (11426346910 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-Myc (WB, 1:2000; 9E10, Cat. #11667149001, Roche; labeled as IR for 293FT cells). Generation and validation of rabbit polyclonal antibodies against GFP was described previously(65 ...
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bioRxiv - Neuroscience 2023Quote: ... Digoxigenin (DIG)-labeled cRNA probes (riboprobes) were prepared using the DIG RNA Labeling Mix (Roche). Twenty μm coronal cryostat sections of mouse brains frozen in OCT were hybridized to DIG-labeled cRNA probes at 56°C for 13–16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Labeled exosomes were diluted with 3.5 ml PBS containing 1x Complete protease inhibitor cocktail (Roche) and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024 ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed probes were purified with Quick Spin Columns for radio-labeled DNA Purification (Roche). ZNF410 full-length or ZNF410-ZF were overexpressed and harvested from COS-7 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... The krang digoxigenin-labeled probe was synthesized in vitro using DIG RNA labeling kit (Roche) with T3 (sense ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA containing EEEb1 was labeled with cyanine-5-dUTP by Nick Translation Mix (Roche), while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10 ...
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bioRxiv - Developmental Biology 2023Quote: ... DIG or fluorescein labeled RNA probes were synthesized by T7 and sp6 RNA polymerases (Roche) from template DNA plasmid digested by NotI or SpeI (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... or 20 μg of fluorescein-labeled probes (see below) for fluorescent detection (Roche, cat. #11685619910). After hybridization ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxygenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling NTP mix (Roche) and either SP6 or T7 RNA polymerase (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized from linearized DNA and labeled during synthesis with digoxigenin-11-UTP (Roche) nucleotides ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The fluorescein-labeled probe was detected using an Anti-Fluor-POD antibody (Roche, Catalog# 11426346910), diluted 1:1500 in Blocking Buffer ...
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bioRxiv - Genomics 2024Quote: ... Specific Hydrangea satDNA probes were labeled indirectly by PCR with biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with a DIG RNA Labeling Kit (Roche Applied Science). The paraffin sections were made as described above and subjected to in situ hybridization ...
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bioRxiv - Genetics 2024Quote: ... Digoxigenin (DIG)-labeled in-situ probes were synthesized using DIG RNA Labeling Kits (Roche, 11277073910). RNA in-situ hybridization was carried out as previously described (Thisse and Thisse ...
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bioRxiv - Neuroscience 2019Quote: ... Clear supernatant was incubated with 2 ml of pre-equilibrated Ni-beads (cOmplete™ His-Tag Purification Resin, Roche, Switzerland) for 1 h and then transferred to a sigma column to be washed consecutively with His-binding buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... and the presence of SnASFF1-HT was confirmed by immunoblot using the BMG–His-1 monoclonal antibody (Roche, Basel, Switzerland). Purified SnASFF1 proteins were stored in 100 mM sodium acetate (pH 4.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
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bioRxiv - Genomics 2024Quote: ... followed by a cell lysis with Hi-C lysis buffer (10 mM Tris-HCl [pH 8.0], 10 mM NaCl, 0.2% NP-40, 1x Roche protease inhibitors) for 30 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014 ...
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bioRxiv - Evolutionary Biology 2019Quote: 15-20 µg of total RNA was digested with DNase I (recombinant, RNase-free, Roche Life Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: Mice livers were perfused and dissociated into single cells using Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) as previously described8 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein G beads (Roche) were added for 2 h ...
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bioRxiv - Genetics 2020Quote: ... Protein A beads (Roche) were added for 2h ...
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bioRxiv - Immunology 2020Quote: ... Protein A agarose (Roche) beads were equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... protein inhibitor cocktail (Roche cOmplete Ultra ...
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bioRxiv - Developmental Biology 2023Quote: ... RT qPCR samples were prepared using the KAPPA SYBR R fast (Roche, KK4611). Primer Sequences:
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The resulting lysate was cleared by centrifugation (13 000 g, 40 min, 4°C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4°C with gentle agitation ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or FITC-labeled antisense probes were synthesized using an RNA Labeling Kit (SP6/T7; Roche). The staining was revealed with NBT/BCIP or INT/BCIP substrate (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled antisense Rp-BicC RNA probes were synthesized using the RNA-Dig Labeling kit (Roche). In situ hybridization was carried out in 4% PFA fixed ovaries stored in PBT at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... the DIG-labeled probe was visualized by using alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics) and Fast Red (Roche Diagnostics) ...
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bioRxiv - Neuroscience 2019Quote: ... Dioxygenin (DIG)-labeled probes were detected using peroxidase-conjugated anti-DIG antibodies (Roche Diagnostics, Mannheim, Germany). Peroxidase activity was detected using fluorescein-tyramide conjugate ...
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bioRxiv - Plant Biology 2022Quote: ... gene-specific cDNA was amplified and labeled using the DIG RNA Labeling Kit (Roche, Basel, Switzerland). Pretreatment of sections ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization with digoxigenin (DIG)-labeled antisense RNA probes was conducted in DIG Easy Hyb (Roche Diagnostics) at 68°C ...
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bioRxiv - Physiology 2022Quote: ... Digoxigenin (DIG) labeled probe was synthesized using the DIG RNA labeling kit (SP6/T7) (Roche, #11175025910). Denatured DIG-labeled probe was hybridized at 55° C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Visualization of the labeled probe was done using anti-DIG-AP (alkaline phosphatase) antibodies (Roche Biochemicals) followed by histochemical enzymatic staining reagents (Roche Biochemicals) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequences were amplified from cDNA and DIG-labeled RNA probes complementary to the target mRNA (Roche). The primer sequences for synthesizing the probes are available in supplementary table 4 ...
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bioRxiv - Genetics 2019Quote: ... Digoxigenin-labeled RNA probes were prepared using a DIG Northern starter kit (Cat. No. 2039672, Roche) following the manufacturer’s instructions ...