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Citations for Roche :
451 - 500 of 1377 citations for Recombinant Human CD19 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Chloroform extracted RNA was treated with RNase free recombinant DNase I (047 716 728 001, Roche) for 1 h at 37°C using 2 units/μg of RNA ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The embryos were incubated in 50 μL of recombinant Proteinase K (Roche Holding AG, Basel, Switzerland) diluted to ∼2 mg/mL in 1 x tris-EDTA (TE ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...
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bioRxiv - Cell Biology 2021Quote: ... Urine glucose levels were measured with a COBAS(r) 2000 analyzer (Roche). Blood glucose levels were measured using an “On Call GK dual” glucometer (Robe Medical ...
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bioRxiv - Cell Biology 2020Quote: ... Protein G-agarose and protein A-agarose beads were from Roche. GFP-Trap®-A beads were from Chromotek (Planneg-Martinsried ...
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bioRxiv - Biophysics 2019Quote: ... We employed fibronectin (FN, from human plasma, Roche Applied Science ...
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bioRxiv - Neuroscience 2022Quote: ... The media for human NPCs obtained from Roche was supplemented with B-27-supplement minus vitamin A (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... human NEMO (5µg) and ATP (2mM) (Roche, 1051997900) were incubated in a reaction buffer consisting of 50mM HEPES (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Human IFN-α2a (Roferon) was purchased from Roche. 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of human genomic DNA (11691112001, Roche) was tagmented in 25 μl reactions by adding 12.5 μl of 2X tagmentation buffer (20mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Human gDNA of high molecular weight (Roche Diagnostics) and ssDNA (M13mp18 single-stranded virion DNA ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... for synthesis of DIG-labeled probes or Fluorescein RNA Labeling Mix (Roche; Cat. No. 1685619) for synthesis of fluorescein-labeled probes ...
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bioRxiv - Neuroscience 2021Quote: ... and premixed RNA labeling nucleotide mixes containing fluorescein-labeled UTP (Roche Diagnostics, Indianapolis, IN, USA). Riboprobes were purified using G-50 spin columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with labeled probe and 250 µg/mL salmon sperm DNA (Roche) at 65°C overnight in Perfecthyb Plus Hybridization Buffer (Millipore) ...
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bioRxiv - Developmental Biology 2019Quote: DIG-labeled antisense riboprobes for the below genes were generated using DIG Labeling Kit (Roche). In situ hybridization was performed as described (Swift et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Labeled antisense RNA probes were transcribed from linearized DNA using digoxigenin-ll-UTP (Roche, USA) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2019Quote: ... Labeled antisense RNA probes were transcribed from linearized DNA using digoxigenin-11-UTP (Roche, USA), or labeled with DNP (Mirus ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescein labeled probes were detected using an alkaline-phosphatase conjugated anti-fluorescein Fab2 antibody (Roche), followed by BCIP/NBT (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... except Dig-labeled probes were detected with HRP-conjugated α-Dig (Roche, 11207733910, 1:500) in conjunction with the TSA system (Molecular Probes) ...
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bioRxiv - Neuroscience 2019Quote: ... Fluorescein-labeled probes were detected using peroxidase-conjugated anti-fluorescein antibodies (Roche Diagnostics, Mannheim, Germany). Peroxidase activity was detected using Cy3-tyramide conjugate ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then probed with alkaline phosphatase labeled anti-DIG antibody (Roche, Catalog# 11093274910) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then fixed again and hybridized with digoxigenin (DIG)-labeled probes (Roche, Basel, Switzerland) at 72°C overnight in hybridization solution (50% formamide ...
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bioRxiv - Neuroscience 2020Quote: In situ hybridization was performed using digoxigenin-labeled RNA probes (DIG RNA labeling kit, Roche) and alkaline phosphatase revelation (NBT/BCIP kit #SK-5400 ...
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bioRxiv - Genetics 2021Quote: ... DIG-labeled in-situ probes were synthesized using DIG RNA Labeling kits (Roche, Cat# 11277073910). RNA in-situ hybridization was carried out as previously described (Thisse and Thisse ...
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bioRxiv - Zoology 2020Quote: ... Each gel also contained 32P-end-labeled size ladders (Molecular Weigh Marker XV, by Roche Diagnostics and 1Kb DNA ladder by New England Biolabs) ...
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bioRxiv - Plant Biology 2020Quote: ... Digoxigenin-labeled sense and antisense RNA probes were synthesized following manufacturer’s instructions (Roche Applied Science). Mature green fruits (21 DAA ...
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bioRxiv - Genetics 2020Quote: ... Digoxigenin-labeled probes were generated from linear plasmid DNA using DIG RNA Labeling Mix (Roche) and hybridized probes were detected with anti-digoxigenin antibody (Anti-Digoxigenin-AP ...
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bioRxiv - Microbiology 2021Quote: ... cells were labeled beforehand for 30 minutes with an anti HA antibody (3F10, Roche 11867423001) diluted at 1:200 in PBA ...
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bioRxiv - Developmental Biology 2022Quote: ... digoxigenin-labeled probes were recognized using an anti-DIG antibody coupled to peroxidase (11207733910, Roche), and fluorescein-labeled probes were recognized using an anti-FLUO antibody coupled to peroxidase (11426346910 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-Myc (WB, 1:2000; 9E10, Cat. #11667149001, Roche; labeled as IR for 293FT cells). Generation and validation of rabbit polyclonal antibodies against GFP was described previously(65 ...
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bioRxiv - Neuroscience 2023Quote: ... Digoxigenin (DIG)-labeled cRNA probes (riboprobes) were prepared using the DIG RNA Labeling Mix (Roche). Twenty μm coronal cryostat sections of mouse brains frozen in OCT were hybridized to DIG-labeled cRNA probes at 56°C for 13–16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Labeled exosomes were diluted with 3.5 ml PBS containing 1x Complete protease inhibitor cocktail (Roche) and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024 ...
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bioRxiv - Developmental Biology 2023Quote: ... The krang digoxigenin-labeled probe was synthesized in vitro using DIG RNA labeling kit (Roche) with T3 (sense ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA containing EEEb1 was labeled with cyanine-5-dUTP by Nick Translation Mix (Roche), while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10 ...
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bioRxiv - Developmental Biology 2023Quote: ... DIG or fluorescein labeled RNA probes were synthesized by T7 and sp6 RNA polymerases (Roche) from template DNA plasmid digested by NotI or SpeI (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... or 20 μg of fluorescein-labeled probes (see below) for fluorescent detection (Roche, cat. #11685619910). After hybridization ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxygenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling NTP mix (Roche) and either SP6 or T7 RNA polymerase (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized from linearized DNA and labeled during synthesis with digoxigenin-11-UTP (Roche) nucleotides ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed probes were purified with Quick Spin Columns for radio-labeled DNA Purification (Roche). ZNF410 full-length or ZNF410-ZF were overexpressed and harvested from COS-7 cells ...
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Neuroscience 2019Quote: ... Clear supernatant was incubated with 2 ml of pre-equilibrated Ni-beads (cOmplete™ His-Tag Purification Resin, Roche, Switzerland) for 1 h and then transferred to a sigma column to be washed consecutively with His-binding buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... and the presence of SnASFF1-HT was confirmed by immunoblot using the BMG–His-1 monoclonal antibody (Roche, Basel, Switzerland). Purified SnASFF1 proteins were stored in 100 mM sodium acetate (pH 4.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...