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Citations for Roche :
5151 - 5200 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The short-insert paired-end libraries for the whole genome sequencing were prepared with KAPA HyperPrep kit (Roche Kapa Biosystems) with some modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... The short-insert paired-end libraries for the whole genome sequencing were prepared with KAPA HyperPrep kit (Roche Kapa Biosystems) with some modifications ...
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bioRxiv - Genetics 2021Quote: We extracted DNA from nail samples using the ISOHAIR (Nippon Gene) and oral mucosa samples using the High Pure PCR Template Preparation Kit (Roche).
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bioRxiv - Genomics 2020Quote: Barcoded 2×300 bp metagenomic libraries were prepared with the KAPA Hyperplus Library Preparation kit (KAPA Biosystems, Wilmington, MA, US) and sequenced on an Illumina MiSeq system at the National Oceanography Centre (Southampton ...
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Cancer Biology 2021Quote: Reverse transcription for qPCR analysis was done with the first strand synthesis kit (Fermentas) using oligo (dT)18 as a primer and qPCR was carried out with LightCycler 96 (Roche) using FastStart Essential DNA Green Master Mix (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: The in vitro synthesis of digoxigenin (DIG)-labeled antisense SatIII probe (158 bp SatIII repeat cloned in 1μg of PGEM-2-98 construct) with T7 RNA polymerase was carried out using the DIG labeling kit from Roche Products Pvt ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with aKAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In-house sequencing adaptors were ligated to 100-300 bp fragments of input dsDNA ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using the LightCycler 480 Probe Master Kit and the LightCycler 480 II system (Roche Applied Science) as described previously92 ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Illumina compatible Indexed libraries were prepared from 12ng of Diagenode Biorupter Pico sheared ChIP DNA using the KAPA Hyper Library Preparation Kit (Roche). Libraries were enriched with 2 cycles of PCR then assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Triplicate reactions that amplified were pooled together and quantified with Kapa library quantification kit (Kapa Biosystems, KK4824, Wilmington, MA, USA) before equimolar sample mixing ...
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bioRxiv - Immunology 2020Quote: ... The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Equimolar amounts of each library were sequenced on a NextSeq 500 instrument controlled by the NextSeq Control Software (NCS ...
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bioRxiv - Immunology 2020Quote: ... Amplifications were carried out on a LightCycler 480 II instrument using LightCycler 480 software (v. 1.5.0) and a LightCycler 480 SYBR green I master kit (Roche Applied Science) according to the provided instructions ...
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bioRxiv - Immunology 2021Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Immunology 2020Quote: ... finally hybridized for 3h at 42 °C with DIG-labelled telomere probes and bulk telomere length analysed as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). For TRF on APC vesicle preparations ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from homogenized tissue of adenovirus vectored vaccine inoculated mice by High Pure Viral Nucleic Acid Kit (Roche). DNA fragments of Sad23L and Ad49L vectors were amplified by nested PCR with primers specific to adenoviral hexon sequences (Table S4 ...
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bioRxiv - Immunology 2021Quote: ... Poly A mRNA was purified from total RNA using oligodT magnetic beads and strand-specific indexed libraries were prepared using the Illumina’s TruSeq Stranded mRNA Sample Prep Kit followed by ten cycles of amplification using KAPA HiFi DNA polymerase (KAPA Biosystems). Libraries were quantified and pooled based on a post-PCR Bioanalyzer and 75 base pair (bp ...
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bioRxiv - Immunology 2021Quote: ... Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table) was labeled with 32P (Amersham) using the High Prime labeling kit (Roche) according to manufacturer’s instructions and unincorporated 32P was removed with G-50 Sephadex Quick Spin columns (Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Telomere dot blot was performed by 3h hybridization at 42°C with DIG-labelled telomere probe as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). Control Input was 200 ng genomic DNA from APCs ...
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bioRxiv - Immunology 2021Quote: Sample RNA extraction was performed from freshly isolated C57BL/6J WT pMBEMCs following the manufacturers instruction of the High Pure RNA Isolation Kit (Hoffman-La Roche). cDNA was obtained from total isolated RNA of each sample with the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Quantification and quality control of the final HiChIP library was conducted using a Bioanalyzer and KAPA quantification kit (Kapa Biosystems). Libraries underwent Next Generation Sequencing on a HiSeq 2500 generating 100 bp paired-ends.
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bioRxiv - Neuroscience 2021Quote: ... Alkaline hydrolysis was then performed at 60°C for 30 min to fragment the target RNA before labeling it with Digoxigenin and Fluorescein according to kit instructions (Roche).
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bioRxiv - Microbiology 2021Quote: ... Library concentration for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Molecular Biology 2022Quote: ... The clones were washed off the plates and the total plasmid was extracted using High-pure plasmid isolation kit (Roche).
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bioRxiv - Cell Biology 2022Quote: ... The membrane was hybridized with a TAA(CCCTAA)4 probe which was conjugated with digoxigenin (DIG oligonucleotide 3⍰-end labelling kit, Roche) and signal was revealed using the anti-DIG-alkaline phosphatase antibodies (Roche ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The quantity of WSSV genome copies was measured by absolute q-PCR using the primers of WSSV-F/R and a TaqMan fluorogenic probe named TaqMan probe-WSSV via LightCycler TaqMan Master kit (Roche) as described previously (Supplementary Table S2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an additional step 72°C for 10 min performed on a Light Cycler 480 with the SYBR Green I Master kit (Roche) (qPHD UM2/GenomiX Platform ...
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bioRxiv - Microbiology 2023Quote: ... and the membrane was treated with the DIG-High Prime DNA Labeling and Detection Starter kit II (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal; Kapa Biosystems, Woburn, MA).
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: Viral RNA was extracted from 100 µL of tissue or nasal wash samples using MagNA Pure 24 Total NA Isolation Kit on the MagNA Pure 24 system (Roche) and eluted with 50 µL of water ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real time PCRs (qPCRs) were performed in a total volume of 10μl with Kapa SYBR Fast qPCR kit (KAPA Biosystems) on a QuantStudio 3 Real-Time PCR system ...
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bioRxiv - Immunology 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using a FastStart Universal Probe Master (Rox) kit (Roche Applied Science) or Luna Universal Probe qPCR Master Mix (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... labeled antisense riboprobes were generated from the cDNA template using T7 RNA polymerase and a DIG-UTP RNA labeling kit (Roche). Brain sections were fixed in 3% phosphate buffered paraformaldehyde for 5 min followed by a rinse in phosphate buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... the cDNA was amplified using KAPA SYBR fast qPCR kit (Nippon genetics) with LightCycler 480 II system (Roche, Mannheim, Germany). The temperature profile of the reaction was 95 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Afterwards 5ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Immunology 2023Quote: ... lactate dehydrogenase (LDH) was measured in supernatants collected at 48 hours post transfection using cytotoxicity detection kit PLUS Assay (CYTODET-RO, Roche) according to manufacturer’s instructions and plates were read at 490 nm.
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was done on 100ng of DNA using a LightCycler 480 Probe Master-Mix kit (Roche, Cat. No. 04707494001) and a primer and probe set specific to MHV68 gB to quantify the number of viral genome copies ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from sigmoid colon tissue using a High Pure RNA Isolation kit (Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... and in vitro transcribed using T7 (pSC-pbx3a, pSC-cdx1b) or T3 polymerase (pSC-best4, pSC-otop2) and fluorescein or digoxygenin RNA labeling kits (digoxygenin: Roche, Cat# 11277073910 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used a Covaris E220 sonicator to shear DNA and we then prepared genomic libraries using the KAPA HyperPlus kit (Roche). Individual libraries were indexed using KAPA UDI’s and pooled libraries were sent to Novogene for Illumina paired-end 150 bp sequencing on a Novaseq X.
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bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems). Libraries were sequenced on an Illumina NextSeq 550 using 150 nt paired-end sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... Afterwards 5ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing library was prepared using a TruSeq-based protocol (KAPA LTP Library Preparation kit Illumina, catalog num. 7961898001, Roche) and manufacturer protocol was strictly followed ...
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bioRxiv - Immunology 2023Quote: ... cytotoxicity was determined based on the release of lactate dehydrogenase (LDH) into the co-culture supernatant measured by Cytotoxicity Detection Kit (Roche) and using a Synergy 2 Multi-Mode Microplate Reader (Biotek ...