Labshake search
Citations for Roche :
5301 - 5350 of 5370 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... of total and polysomal transcripts was conducted as described in [96] with the difference of using KAPA SYBR® FAST qPCR Kit (Kapa Biosystems, South Africa). ACTIN2 (AT3G18780 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were checked with a Fragment Analyzer (Advanced Analytical Technologies, Inc., Ankeny, IA) and quantified by qPCR using the Kapa Library Quantification Kit (Roche Diagnostics Corp, Indianapolis, IN). Sequencing was performed on a NovaSeq S4 lane (Illumina ...
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bioRxiv - Physiology 2019Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed using Transcriptor High Fidelity cDNA Synthesis Kit and a specific 3’-UTR DENV-1 primer (Roche Applied Science, Mannheim, Germany), d1a5B 5’-AGAACCTGTTGATTCAACRGC-3’ (62) ...
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bioRxiv - Cancer Biology 2019Quote: ... The quality and quantity of the final libraries were determined using the Agilent 2100 Bioanalyzer DNA HS kit and Kapa Biosystems qPCR (Kapa Biosystems, Inc., Wilmington, MA). Multiplexed libraries were pooled and normalized to 17.5 pM ...
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bioRxiv - Genetics 2020Quote: ... The final linked-read library (8 cycles of PCR amplification) was quantified using the Kapa Library Quantification Kit (Cat. No. KK4824) from Kapa Biosystems (Woburn, MA, USA) and diluted to 4 nM ...
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bioRxiv - Plant Biology 2021Quote: ... was isolated from the cDNA of juvenile cluster root tissues from P-deficient plants using the 5’/3’ RACE Kit (2nd generation, Roche Diagnostics S.p.a., Monza, Italy) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA-Seq Library was prepared according to KAPA Stranded mRNA-Seq poly(A) selected kits with 201-300 bp insert sizes (KAPA Biosystems, Wilmington, MA, USA) using 250 ng total RNA as an input ...
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bioRxiv - Genomics 2020Quote: ... nucleic acids were extracted directly from 200 μl CSF sample using the MagNApure 96 DNA and Viral NA Small volume extraction kit on the MagNAPure 96 system (Roche Diagnostics, Almere, The Netherlands) with 100 μL output eluate ...
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bioRxiv - Microbiology 2020Quote: ... the genomic DNA of each isolate was extracted using a MagNA Pure LC DNA isolation kit on a MagNA pure LC instrument (Roche Diagnostics GmbH, Mannheim, Germany), which was used for Nextera XT library construction and genome sequencing (300 bp paired-end ...
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bioRxiv - Developmental Biology 2020Quote: ... Riboprobes were synthesized from the plasmids by in vitro transcription using either T7 or Sp6 polymerase and a digoxigenin (DIG) labeling kit (Roche Applied Science, Indianapolis, IN). The primer sequences used for riboprobe preparation are given in Supplemental Table 1.
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bioRxiv - Molecular Biology 2020Quote: Equal amount by mole of non-targeted and targeted RNA-Seq library were mixed based on qPCR-based quantification with Kapa Library Quantification Kit (KAPA BIOSYSTEMS, Wilmington, MA, USA). Sequencing of 150-bp paired-ends using HiSeq was carried out.
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bioRxiv - Genomics 2021Quote: ... Quantification of final library before loading on MiSeq sequencer was performed using the Kapa quantification kit (RT-PCR) for next generation sequencing with the Illumina platform (Kapa Biosystems, Wilmington, MA USA). Sequence reads were quality filtered and assembled using CLC Genomics Workbench 8.0.1 (CLCbio ...
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bioRxiv - Genomics 2021Quote: ... Sense and antisense digoxigenin-labelled RNA probes were generated by in vitro transcription using the DIG-RNA Labeling Kit (SP6/T7; Roche Applied Sciences, QC, Canada). Labeled RNA probes were quantified by dot blot analysis.
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bioRxiv - Genetics 2020Quote: ... Tag sequences for identifying individual trees were attached to the DNA fragments using the Access Array Barcode Library for Illumina Sequencers-384, Single Direction (Fluidigm Corporation, South San Francisco, CA, USA) and KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, Wilmington, MA, USA). PCR was performed in a 20 μL volume containing 2 μL of 20-times diluted PCR products ...
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bioRxiv - Microbiology 2022Quote: ... IHC tissues were processed using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760-159) for immunohistochemistry (IHC ...
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bioRxiv - Neuroscience 2022Quote: ... tissue samples were taken by collecting ∼2 mm of the end of the tail and extracting DNA from the tissue sample using the Kapa Express Extract Kit (Kapa Biosystems, Wilmington, MA, USA). The extracted DNA was then subjected to PCR using in-house designed primers to identify WT and KO alleles ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... n=40) and brain (n=40) for each individual using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adapters (KAPA Biosystems; Wilmington, MA, USA), with 100 ng of large RNA as input ...
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bioRxiv - Genetics 2022Quote: ... and amplifying the exon regions of targeted genes by using the primers with SP6 sequence (ATTTAGGTGACACTATAGAAGNG) according to the product description of KAPA HiFi PCR Kit (Roche Diagnostics, cat. no. 07958927001). Sense and antisense digoxigenin (DIG)-labeled probes were synthesized from the purified PCR product using DIG RNA Labeling Kit (Roche ...
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bioRxiv - Genetics 2022Quote: ... The hygromycin cassette including the B-tubulin promoter and terminator was amplified from pPHT1 plasmid with the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... first purified with two rounds of polyA mRNA selection with oligo-dT magnetic beads and then converted to sequencing-compatible cDNA using the KAPA mRNA HyperPrep kit (KAPA biosystems, Cat. No. KK8580), using the KAPA single-indexed adapter kit for multiplexing (KAPA biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cDNA was used for qRT□PCR analyses using the TransStart® Green qPCR SuperMix kit (AQ101-01, TransGen Biotech, Beijing, China) on a LightCycler® 480 System (Roche, Basel, Switzerland). Ubiquitin 5 was selected as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... Final libraries were analyzed using Agilent DNA 1000 chip to estimate the quantity and check size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit (ref. KK4835; Kapa Biosystems, Wilmington, MA, USA) prior to amplification with Illumina’s cBot ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Extracted DNA samples were sent to Admera Health (South Plainfield, NJ) and were prepared using the KAPA HyperPrep kit (Kapa Biosystems, Inc., Wilmington, MD) according to the provided instructions ...
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bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (5∼8 μg) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We verified enrichment success and measured size-adjusted DNA concentrations of each pool with qPCR using a SYBR-FASTqPCR kit (Kapa Biosystems, Wilmington, MA, U.S.A.) and a Bio-Rad CFX96 RT-PCR thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were processed for DNA strand breaks using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) from Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN, US) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA from each sample was processed using the KAPA Stranded mRNA-Seq Kit with mRNA capture beads (Kapa Biosystems, Bedford row, London). Each sample was then eluted in 20 μL of elution buffer adjusted to 10 mM ...
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bioRxiv - Neuroscience 2023Quote: Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... To correct ONT reads another library using the same HMW sample as above was constructed for shotgun sequencing with the Hyper Library construction kit from Kapa Biosystems (Roche, Basel, Switzerland) and sequencing was performed using an Illumina NovaSeq at the Roy J ...
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bioRxiv - Microbiology 2023Quote: ... were ligated to the individual cDNA products to barcode the samples and Kapa HiFi HotStart Library Amplification kit (kk2612, Roche Sequencing and Life Science) was used for library amplification.
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Plant Biology 2023Quote: ... and purified based on the manufacturer’s instructions before sample barcoding with xGen Stubby Adapter and Unique Dual Index Primers (IDT) and library preparation using KAPA HyperPrep Kit (Kapa Biosystems-Roche, Wilmington, MA). The library was then sequenced by Illumina MiSeq nano PE150 at the Georgia Genomics and Bioinformatics Core ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Physiology 2023Quote: ... mRNA was enriched by poly-A capture and mRNA-seq libraries were generated using KAPA Stranded mRNA-seq Kit (KAPA Biosystems, Wilmington, Massachusetts, USA). For ovulated mature eggs ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... We used identical input total RNA (50 ng to 500 ng) between control and samples for library preparation using the KAPA RNA HyperPrep Kit (KAPA Biosystems, Wilmington, MA, USA). Libraries were analyzed by Agilent 2100 bioanalyzer system for quality control ...
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bioRxiv - Physiology 2024Quote: RT‒qPCR was conducted by using a KAPA SYBR Fast qPCR kit (Nippon Genetics) with a LightCycler 480 II system (Roche Applied Science, Penzberg, Germany). The PCR reactionwas conducted under the following conditions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and ligated to iTru adapters followed by limited-cycle PCR of iTru primers to add indexes and complete the library molecules using Kapa Library Preparation Kit reagents (Kapa Biosystems [Roche, Basel, Switzerland]). We sequenced the pooled libraries on an Illumina sequencer (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Quality of libraries were determined via Agilent 2200 TapeStation using High Sensitivity D1000 tape and quantified using Kappa SYBR®Fast qPCR kit (KAPA Biosystems, Inc, Wilmington, MA). Approximately 60-80 million paired-end 150 np sequence reads were generated per sample using the Ilumina HiSeq 4000 sequencer.
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bioRxiv - Immunology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist.
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bioRxiv - Microbiology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159) (Roche Diagnostics Corp. ...