Labshake search
Citations for Merck :
451 - 500 of 5137 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2019Quote: ... Protein was transferred overnight at 10 V to an Immobilon-P membrane (Merck Millipore; Burlington, MA) in 25 mM Tris ...
-
bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
-
bioRxiv - Neuroscience 2021Quote: ... downward capillary action was used to transfer protein to immune-blot PVDF membrane (Merck cat# IPVH00010). Membranes were blocked with 5% milk in wash buffer (0.05% Tween in PBS ...
-
bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... Purified protein was concentrated to 8 mg/mL using 30 kDa cut-off AMICON concentrators (Merck).
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to ~65 mM using a MWCO 30,000 Amicon Ultra Centrifugal Filter (Merck), flash-frozen and stored at −80 °C until use.
-
bioRxiv - Plant Biology 2021Quote: ... The collected protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut off and frozen at −80 °C.
-
bioRxiv - Biochemistry 2021Quote: ... 500 μg protein was added to spin columns (Pierce) containing anti-FLAG M2 affinity resin (Merck) and allowed to incubate rotating for two hours at 4°C ...
-
bioRxiv - Biophysics 2023Quote: ... the protein was previously filtered with 0.22 µM Millex Syringe filters (Durapore PVDF, Merck KGaA, Germany) in order to exclude the presence of potential protein oligomers or aggregates ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
-
bioRxiv - Cancer Biology 2020Quote: ... the membrane was developed with a peroxidase-conjugated secondary antibody from Merck Millipore ...
-
bioRxiv - Molecular Biology 2019Quote: SMARCAL1 antibody was raised against N-terminus HARP domain by Merck (India) (Catalog # 106014) ...
-
bioRxiv - Cell Biology 2023Quote: ... α-phospho-H3 (Ser10) (catalog no.: 06-570) antibody was purchased from Merck; α-HA tag (16B12 ...
-
bioRxiv - Neuroscience 2024Quote: ... the primary antibody used was a chicken anti-RFP (AB3528, Merck Millipore) diluted 1:300 in blocking buffer for overnight incubation ...
-
bioRxiv - Physiology 2023Quote: ... the membrane was probed with a specific antibody against actin (Merck MAB1501) for normalisation of the radioactive signals of mitochondrial proteins.
-
bioRxiv - Biophysics 2021Quote: ... and Desthiobiotin (71610-3) were purchased from Merck Life Science UK Limited ...
-
bioRxiv - Genetics 2021Quote: ... followed by 3 washes in KSOM (Merck Millipore) medium droplets ...
-
bioRxiv - Cell Biology 2022Quote: ... and Sf9 TriEx (71023-3, Novagen, Merck, UK) were grown at 28°C in a dry incubator without CO2 ...
-
bioRxiv - Cell Biology 2022Quote: ... and Control siRNA Luciferase: 5’ CGUACGCGGAAUACUUCGA 3’ (Merck). HeLa cells were transfected on two consecutive days with 20 nM Cav1 siRNAs using Lipofectamine RNAiMAX (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.5 mM 3-isobutyl-1-methylxanthine (Merck, I5879), 1 μM dexamethasone (Merck ...
-
bioRxiv - Systems Biology 2021Quote: ... We used filter sizes of 3 kDa (Merck, Amicon Ultra-15 Centrifugal Filter Unit ...
-
bioRxiv - Cancer Biology 2020Quote: ... transferred on ice and benzonase (Merck, #71206-3) was added to degrade DNA at 37°C for 30 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Cells were fixed with 3% glutaraldehyde (Merck, 1042390250) in 0.1 M mNa-phosphate buffer (pH 7.4) ...
-
bioRxiv - Bioengineering 2022Quote: ... intralipid (2.08 v/v%; Merck, 68890-65-3) was used to mimic tissue-like scattering conditions and Nigrosin (0.62 v/v% of Nigrosin stock solution [0.5 mg/mL Nigrosin in deionised water ...
-
bioRxiv - Cell Biology 2022Quote: ... and 3 mM EGTA (cat. no. 324626, Merck) was used for 1 h pre-treatments ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 µL of Benzonase (Merck Millipore, US170664-3) was added and samples left on ice until an aqueous solution formed (30 min to 1 h) ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 U/mL benzonase (Merck KGaA, Darmstadt, Germany) per each mL of the original culture were added and the homogenate was incubated for 20 min ...
-
bioRxiv - Biophysics 2023Quote: ... using GeneJuice transfection reagent (Merck, ref: 70967-3) according to manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... 4°C (Merck 3-16KL, KGaA®, Germany). Supernatant was collected and filtered through PTFE membrane filter (0.2 μm ...
-
bioRxiv - Neuroscience 2023Quote: ... 3% bovine serum albumin (BSA, Merck/Sigma-Aldrich) in PBS was added as blocking buffer and incubated for 1 hour ...
-
bioRxiv - Developmental Biology 2024Quote: ... passed through 3 drops of Advanced KSOM (Merck) and kept for 30 minutes in a drop of KSOM (Merck ...
-
bioRxiv - Cell Biology 2024Quote: ... 4-hydroxy-3-methoxy-acetophenone (Apocynin, 73536, Merck), gp-91-ds-tat (AS-63818 ...
-
bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
-
bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
-
bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
-
bioRxiv - Microbiology 2023Quote: ... The remaining 32P-γ-ATP was removed by washing with 3 column volumes of Millipore water and centrifugation in 10 kDa (Qβ-RNA) or 3 kDa (8mer) Amicon filters (Merck Millipore) at 14,000 rpm at 4 °C for four times ...
-
bioRxiv - Neuroscience 2023Quote: ... The AAV titer was quantified using PCR (5′-TGA GTC ACC CAC ACA AAG GA-3′ and 5′-CCA AGC TGG CCT AAC TTC AG-3′) after proteinase K treatment (Merck Millipore). Under anesthesia with a mixture of medetomidine (0.3 mg/kg ...
-
bioRxiv - Cell Biology 2024Quote: ... The activation was achieved using 50 μL of the initial microbead’s solution following the two-step 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide (EDC)/sulfo NHS covalent coupling (Estapor carboxyl-modified dyed microspheres protocol, Merck Millipore) in MES buffer (30 min ...
-
bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
-
bioRxiv - Biophysics 2021Quote: ... Immuno-identification of the samples was done using α-c-Myc rabbit antibody and followed by a secondary HRP-conjugated α-rabbit antibody (Merck). α-Histone H3 rabbit antibody was used as housekeeping protein ...
-
bioRxiv - Microbiology 2019Quote: A sample (10 mL) was removed from the Gambierdiscus culture and filtered through 3-μm membrane (Merck Millipore, Darmstadt, Germany). One hundred microliters of the filtrate ...
-
bioRxiv - Molecular Biology 2021Quote: Heat-mediated antigen retrieval was performed for 3 min at 99°C in a 40mM trisodium citrate (Merck, Darmstadt, Germany) solution ...
-
bioRxiv - Neuroscience 2021Quote: Viability of SH-SY5Y cells after treatments with or without H-LIPEF was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
-
bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
-
bioRxiv - Biophysics 2021Quote: ... The eluted sample was exchanged into 20 mM Tris buffer and concentrated to 5 ml by Amicon Ultra-15 3 kDa (Merck). The sample was purified by 320 ml of HiLoad Superdex with a flow rate of 1 ml/min using the FPLC systems.
-
bioRxiv - Synthetic Biology 2021Quote: ... 100 μL aliquots were removed and the reaction was stopped using a 3 KDa nominal molecular weight limit (NMWL) centrifuge filter (Merck Amicon Ultra 0.5mL Centrifugal Filters ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell medium was replaced with 3 mL medium containing 20 μL of the purified lentivirus and 3μl polybrene transfection reagent (Merck Millipore). Medium was supplemented with 10 µg/mL puromycin for selection of successfully transduced cells two to three days after transduction.
-
bioRxiv - Cell Biology 2022Quote: ... The remaining media was then concentrated down to 500 uL using a falcon tube sized 3 kDa amicon column (UFC900308; Merck) spun at 4000 xg and 4°C for approximately 1 h ...