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Citations for Merck :
701 - 750 of 5137 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... for 3 h or with 100 ng/ml nocodazole (M1404, Merck) for 6 h ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were blocked with 3% bovine serum albumin (Merck Aldrich) in PBST at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibody (Merck guinea pig anti-NeuN 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleaved NlGr7 protein was concentrated by using a 10 kDa cut-off Centricon centrifugal device (Merck Pte. Ltd., Germany) and was purified by affinity chromatography on amylose resin (NEB ...
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bioRxiv - Biophysics 2022Quote: ... and monomeric protein was purified by size-exclusion chromatography using a HiLoad™ 26/600 Superdex™ 75 pg (Merck) equilibrated with 25 mM HEPES ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were digested by addition of porcine trypsin (enzyme to protein ratio of 1:100; for 2 x104 microglial samples, 300 ng was used; Merck) and incubated overnight at 37 °C with shaking ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Biochemistry 2021Quote: ... the pure huPrP protein (i.e., with the HisTag removed) was concentrated using an Amicon Ultra 0.5 ml centrifugal filter (Merck & Co., USA) with an NMWL cutoff of 3 kDa ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using Millex Non-Sterile Low Protein Binding Hydrophilic LCR (PTFE) Membrane (0.45 µm) filters (Merck #SLLHR04NL) into 1.5 mL MS vials with silicone/PTFE septa and stored at -20 °C for later analysis.
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 40 mM Chloroacetamide (CAA) at RT for 30 min and protein amount was quantified using the Direct Detect Spectrometer from Merck. Protein digestion was performed using the Single-Pot Solid-Phase-enhanced Sample Preparation approach SP3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mg of total protein at a 1 mg/ml concentration was incubated with 40 µl anti-FLAG-M2 affinity resin (Merck) (which had been blocked overnight with 1% BSA and 5 µl/ml ssDNA ...
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bioRxiv - Cancer Biology 2019Quote: ... The protein was visualized by exposing the membrane to an X-Omat film with enhanced chemiluminescence reagent (Merck, Darmstadt, Germany). The respective primary antibodies were mouse anti-DRP1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µg of protein solution was mixed with dye at a three-fold molar excess in the presence of NaHCO3 (Merck) buffer at pH 8.3 ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Plant Biology 2023Quote: ... Transfection with plasmids carrying the coding sequences of the indicated proteins was performed using Genejuice transfection reagent (Merck, Darmstadt, Germany). 48 hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of protein was added to each well and run in Boric acid-Tris buffer (192 mM Boric acid, Merck; 1 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The lysozyme protein solution was filtered by a 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA). The filtered sample was stored in 1.0 mL aliquots at −45 °C until crystallization experiments were performed ...
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bioRxiv - Plant Biology 2023Quote: ... alkylation and tryptic digestion (50 µg of protein per sample) was performed in centrifugal filters (Amicon Ultra-0.5, 30 kDa cut-off, Merck Millipore) according to the FASP protocol (Wiśniewski et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated to 60 µM – 100 µM using an Amicon Ultra Centrifugal Filter Unit (30 kDa MWCO, Merck) and stored at −80 °C.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of 50 mM EDTA was added to the reaction mixture and unchelated radioactive copper ion was removed by centrifugation using Amicon® Ultra centrifugal filters (regenerated cellulose, 3 kDa) (Merck Millipore, Billericia, MA). The radioactivity of 64Cu complexed NOTA-POx was measured in gamma counter prior to micelle preparation.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HPLC purification of the (S,S)-MLN-4760 was based on the comparison with the commercially available compound (MLN-4760; MW: 428.31; Merck, CAS N° 305335-31-3). The isolation of the desired (S,S)-diastereoisomers of the respective fluorinated MLN-4760 derivatives was based on the assumption that the HPLC elution sequence of the diastereoisomers would be identical to that of the MLN-4760 lead compound.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was captured on the chip by a previously immobilized anti-FLAG antibody (Merck Life Science S.r.l). S protein (Euprotein) ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibody incubation was followed by 0.1% TBST wash and developed using an ECL kit (Merck). Densitometry analysis was used for quantitation of protein expression levels using Image J ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-integrin β1 antibody (clone HUTS-4) was obtained from Chemicon (Merck Millipore, Billerica, MA). Rabbit polyclonal antibodies to fibronectin and fibrinogen were from Abcam (Cambridge ...
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bioRxiv - Molecular Biology 2021Quote: ... Modified and non-modified PCNA was detected using ANTI-FLAG® M2-Peroxidase (HRP) antibody (Merck).
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bioRxiv - Microbiology 2022Quote: ... The anti-Coxsackievirus B3 monoclonal antibody was obtained from Merck (clone 280-5F-4E-5E, MAB948). The human codon-optimized T7 polymerase plasmid was obtained from Addgene (#65974 ...
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bioRxiv - Cell Biology 2023Quote: DNA-RNA Immunoprecipitation was performed using Anti-DNA-RNA Hybrid Antibody S9.6 (Merck Cat No MABE1095) as per Sanz and Chédin ...
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bioRxiv - Neuroscience 2023Quote: ... Rabbit polyclonal antibodies against ARHGEF2 phosphorylated at Thr-103 was produced against phosphopeptide C+LLRNNpTALQSVS (Merck) as previously described57 ...
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bioRxiv - Biophysics 2024Quote: ... H2A was detected using anti-H2A primary antibodies (Merck Millipore Cat. # 07-146, 1:1000 titer) and HRP-conjugated secondary antibodies (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were mouse anti-EGFR antibody (#GR01; Merck Millipore), goat anti-EEA1 (#sc-6414 ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were concentrated with Centricon filters (Merck-Millipore) when needed and/or diluted to a final concentration of 1 mg/mL in PBS.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected by using Luminata Crescendo (Merck) and LAS600 (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto PVDF membranes (Merck) and blocked with 5% (wt/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to PVDF membrane (#IPFL00010, Merck) for 3 hours at 60 V with Tris/Boric buffer at 50 mM/50 mM ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred onto PVDF membranes (Merck Millipore) by wet electroblotting for 1 h at 100 V using the Mini-PROTEAN Tetra system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred to PVDF membranes (Merck).
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected using chemiluminescence HRP substrate (Merck). Densitometric protein quantifications were carried out by ImageJ.
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to immobilon PVDF (Merck, IPVH20200) membranes at 100 V ...
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bioRxiv - Immunology 2020Quote: ... Samples were precleared with Protein G Sepharose (Merck) for 30 min ...