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Citations for Merck :
1 - 50 of 5137 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and a 1:500 dilution of an antibody against the phosphorylated form of H2A histone family member X (γH2Ax) (clone JBW301, Merck) and 1:200 phalloidin coupled to Alexa Fluor 568 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Cell Biology 2023Quote: ... Biotin food was prepared by adding 200 mM biotin (Merck, B4501) to hot (∼60 ℃ ...
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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bioRxiv - Plant Biology 2021Quote: ... 14-3-3 binding was detected using anti-GST monoclonal antibody (Merck).
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bioRxiv - Neuroscience 2020Quote: ... The antibody aggregates consisted of goat anti-biotin (#B3640; Merck, Germany) and STAR635P-conjugated donkey anti-goat (#ST635P ...
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bioRxiv - Biophysics 2020Quote: ... was labeled with Biotin-NHS (Merck, 203112). Therefore 100 µg of 1g/l primary antibody were treated according to the labeling protocol above using 250 µg of Biotin-NHS ...
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bioRxiv - Biochemistry 2021Quote: ... and the protein was concentrated using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). The protein concentration was calculated using the measured absorbance at 280 nm under native conditions and assuming an extinction coefficient of 19940 M-1 cm-1.
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Biophysics 2023Quote: ... The final purified protein was concentrated to 1 mM using Amicon (3 kDa cutoff, Merck) and exchanged with NMR buffer D (10 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... media was replaced with fresh media containing 50µM Biotin (Merck) and the cells were incubated for a further 24 or 6 hours (for BirA*-RAB18 and BioID2(Gly40Ser)-SEC22A experiments respectively) ...
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bioRxiv - Biophysics 2023Quote: ... to Biotin (Merck Millipore) in a molar ratio of 1:3.5 at 500 µM Streptavidin and 1,750 µM Biotin in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... D-biotin (B4501; Merck) at a final concentration of 500 µM was added to induce the RUSH.
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bioRxiv - Plant Biology 2022Quote: ... and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa: Merck, Darmstadt, Germany) and stored at −20 °C in dialysis buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... The pure protein obtained was concentrated using a 3 kDa Amicon® Ultra Centrifugal Filter (Merck).
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bioRxiv - Systems Biology 2020Quote: ... biotin (13 μM, Merck, B4639) was added for protein biotinylation ...
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bioRxiv - Physiology 2023Quote: ... and biotin-conjugated IB4 (Merck) followed by Alexa Fluor 488-conjugated streptavidin (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... The bound protein complexes were washed thrice with NETN buffer and then eluted with 2 mg/mL biotin (Merck) for 90 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore ...
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bioRxiv - Immunology 2020Quote: Antibody to Ubiquitinated proteins (FK2) from Merck-Millipore was used for the immunoprecipitation assay.
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bioRxiv - Molecular Biology 2023Quote: ... the anti-S tag antibody (Merck, Cat# 71549-3) was added directly to the medium of each well resulting in a final dilution of 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Acetylated histone 3 was detected using a polyclonal rabbit antibody (Merck, 06-599, 3260200, 1:500) and visualized using a donkey anti-rabbit Alexa Fluor 488 secondary antibody (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were then blocked with protein block (3% BSA fraction V (Merck) or 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Free biotin was removed with spin filters with a 10kDa cutoff (Merck, Millipore).
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bioRxiv - Pathology 2021Quote: ... Biotinylated rhIL5 was produced using the Biotin-X-NHS system (Merck, Darmstadt, Germany). A total of 25 μL of rhIL5 (100 μg/mL ...
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bioRxiv - Genetics 2023Quote: ... Anti-S6 Ribosomal Protein Antibody (used with 1:1000 dilution) was purchased from Merck (#ZRB1172). α-tubulin antibody (used with 1:5000 dilution ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Plant Biology 2024Quote: ... the purified protein was concentrated by centrifugation in 3 kDa cut-off filters (Amicon Ultra-15 Centrifugal Filter Units, Merck) at 4,500 x g ...
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bioRxiv - Plant Biology 2024Quote: ... The purified protein was concentrated by centrifugation in 3 kDa cut-off filters (Amicon Ultra-15 Centrifugal Filter Units, Merck) at 4500 × g ...
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bioRxiv - Plant Biology 2024Quote: ... the purified protein was concentrated by centrifugation in 3 kDa cut-off filters (Amicon Ultra-15 Centrifugal Filter Units, Merck) at 4,500 x g ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Fmoc-Lys(Biotin)-OH (4 eq; Merck) in 6 ml DMSO:NMP (1:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse monoclonal anti-biotin (Merck, BN-34), Mouse monoclonal anti-PARP1 (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 mM azide-PEG3-biotin conjugate (Merck) and 2 mM copper sulfate (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to basement membrane proteins collagen-IV (Merck-Millipore) or perlecan (Abcam) ...
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bioRxiv - Bioengineering 2019Quote: ... Eluted proteins were dialyzed using a 3 kDa membrane D-Tube Dialyzer (Merck, Germany) to remove the imidazole in Storage Buffer (200 mM ammonium acetate ...
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bioRxiv - Microbiology 2023Quote: ... was added to the fox and wolf samples and antiferret IgG biotin (Merck, Darmstadt, Germany) was added to the mustelid samples ...
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bioRxiv - Cell Biology 2021Quote: Cells were incubated with 50 µM biotin (Merck) for 16 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 2mM ATP and 150µM D-Biotin (both Merck) and incubated twice for one hour at 30 °C with additional fresh biotin and GST-BirA added in-between incubations ...
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bioRxiv - Immunology 2022Quote: ... Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody clone 6C5 (Merck Millipore, MAB374) was used as control antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Molecular Biology 2019Quote: ... was washed 3 times with PBS (Merck Millipore ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated in primary antibodies solution (antibody diluted in PBS containing 3% goat serum, 0.01% Triton x-100 (Merck)) at 4°C over night ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM Cu2SO4 and 0.1mM biotin-azide (Merck, 762024) or AlexaFluor 647 azide (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... Oocytes were then incubated in 20 μM Biotin (Merck) at 18°C for 40 h ...