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Citations for Merck :
401 - 450 of 642 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 4561086) and transferred at 4°C to either Immobilon-FL or Immunoblon-P (IPVH00010) polyvinylidene fluoride membranes (Merck, IPFL00010) (1.5 hours ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated at 37°C for 1 hour with 10U/ml DNase (Benzonase, Merck-Millipore via Sigma-Aldrich) followed by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... was added for 16-18 hr at 4°C before washes and the addition of anti-phospho H2A.X mAb (Merck, Kenilworth ...
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bioRxiv - Biophysics 2023Quote: ... Poly-D-lysine coating was done by incubating the wells for 20 minutes at 37°C in a 0.4 mg/ml solution (4X) of poly-D-lysine (Merck, P7280-5MG ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) and then transferred to 1.5 mL tubes prewarmed at 65°C acid-washed glass beads (G8772-100 Merck) suspended in 25 µL of 10% filtered SDS and 300 µL of acid phenol chloroform pH 4.5 ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature (RT) and permeabilised overnight at 4 °C in 0.5% Triton X-100 (Merck) solution in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... MLg mouse lung fibroblasts (CCL-206, ATCC) were proliferation-inactivated by incubation in culture medium containing 10-μg·ml-1 mitomycin C (Merck) for 2 h ...
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bioRxiv - Microbiology 2023Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Biochemistry 2023Quote: ... at 4°C (repeated for 10 cycles) to remove salt using an Amicon Ultra-0.5 Centrifugal 10 kDa filter unit (Merck), before dilution to 5.00 µM in 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration (4°C, 3,500 x g) using Amicon Ultra Centrifugal Filter Units 10K molecular weight cut-off (MWCO) (Merck). CT produced in E ...
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bioRxiv - Microbiology 2024Quote: ... samples maintained at 4°C were eluted through a ZIC-pHILIC column (5 μm, polymeric, 150 by 4.6 mm; SeQuant, Merck) by mobile phase A (20 mM ammonium carbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was then spun at 20,000 g for 20 min at 4°C and loaded to a HisTrap HP column (Merck). The protein was eluted with the buffer with 300 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Biochemistry 2024Quote: The lysate was cleared by centrifugation at 12,000g for 30min at 10°C and the supernatant was applied onto a Talon Superflow beads batch (Merck). The immobilized proteins were washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: Unless otherwise noted the standard assay was carried out at 65 °C for 10 min in 500 μL reaction mixture using 0.25 % (w/v) laminarin from Laminaria digitata (Merck) as substrate in 50 mM sodium phosphate buffer pH 6.0 (adjusted at assay temperature) ...
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bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Cell Biology 2019Quote: ... cut axially using a size 10 surgical scalpel into 1mm3 pieces and incubated at 37°C for 45 mins in LiberaseTM (Merck) and 10ul/ml DNAse I (Thermo Scientific™) ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Molecular Biology 2021Quote: Heat-mediated antigen retrieval was performed for 3 min at 99°C in a 40mM trisodium citrate (Merck, Darmstadt, Germany) solution ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% milk in TBS-T and incubated overnight at 4°C with the primary antibodies anti-tyrosine hydroxylase (1:1,000; AB152; Merck Millipore) or anti-dopa decarboxylase (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
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bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
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bioRxiv - Microbiology 2019Quote: ... coli strain strains were grown at 37°C in Lysogeny broth (LB) with shaking at 200 rpm or on agar (Merck). All meningococcal strains were grown on GC agar plates or GC broth (Difco ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Neuroscience 2021Quote: ... After washing the sections were incubated overnight at 4°C with anti-NeuN antibody (1:1000, Merck Millipore, Cat. # ABN90P) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in PBS/ 0,05%Tween20 containing 5% skim milk and then probed with the following primary antibodies over night at 4°C: mouse anti-P53 (1:100, Merck), rabbit anti-H2AX (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% bovine serum albumin (BSA) 30 min at 37°C and incubated with primary anti-γH2A.X mouse antibodies (Merck Millipore) or rabbit anti-LC3B antibodies (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Cell Biology 2021Quote: ... cut axially using a size 10 surgical scalpel into 1mm3 pieces and incubated at 37°C for 45 mins in Liberase (Merck) and 10ul/ml DNAse I (Thermo Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... The reaction was incubated at 25°C for 90 minutes followed by addition of 1 μL proteinase K (Merck, 3115887001) and incubation at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... then dialysed against the same stock of ITC buffer overnight at 4°C using 1 kDa Pur-a-lyzer tubes (Merck). Protein and RNA concentrations after dialysis were calculated by A280 and A260 absorbance respectively ...
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bioRxiv - Physiology 2020Quote: ... blocked in 5% NGS and 0.5% Triton diluted in PBS and incubated over night at 4°C with primary antibody: rabbit anti-NG2 (1/50, AB5320, Merck), goat anti-PDGFRα (1/200 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in TBS and demi-water before being dried on a heat plate at 37°C before and cleared in xylene and mounted with Entellan (Merck).
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bioRxiv - Cell Biology 2020Quote: ... pre-cleared extracts (2 mg) were incubated for 4 hr at 4 °C with 2 μg of pan–ADP–ribose binding reagent (MABE1016, Merck) or normal rabbit IgG (2729S ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: HEK293T cells were cultured in 10 cm tissue culture treated dishes grown at 37 °C in a 5% CO2 atmosphere in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... Filters were incubated in a cold room (4°C) overnight with primary antibodies diluted in PBS with 0.1% TWEEN® 20 (Merck) solution (1:1000 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... protoplasts were overlaid with ½-strength PDA cooled-down to ~50°C and supplemented with 50 mg/ml hygromycin B (Merck). Transformants appearing on the PDA surface ...
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bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Microbiology 2021Quote: ... The entire 20 l volume was then filtered through a GF/C filter (Whatman, Maidstone, United Kingdom) and 0.45 µm PVDF filter (Durapore, Merck Millipore) to eliminate cells and cellular debris.
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bioRxiv - Microbiology 2022Quote: ... “treated” cultures were grown with the addition of 0.25 µg ml-1 mitomycin C (MMC) (Merck Life Sciences UK Ltd). Mycelial pellets from untreated and treated cultures were washed in 1x PBS before lysis and RNA purification using the RNEasy Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... Cecum content was collected from freshly sacrificed mice and stored at -70 °C in anaerobic Brain Heart Infusion (BHI) broth (Merck) containing 20% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... Nuclei were pelleted by centrifugation at 3000 rpm for 5 min at 4°C and were resuspended in hypotonic buffer containing 1U/µl of benzonase (Merck). Extracts were incubated on ice for 30 min ...