Labshake search
Citations for Merck :
551 - 600 of 642 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted at 460 g at 4 °C for 30 s and concentrated in a 1% GDN pre-equilibrated 30 kDa Amicon Ultra centrifugal filter (Merck Millipore). The concentrated eluate was spun through a Zeba Spin Desalting Column 40 kDa MWCO (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
-
bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
-
bioRxiv - Microbiology 2023Quote: ... Cultures were grown in 25% TSB medium at 28°C in static conditions in glass microcosms with one layer of ColiRoller glass beads (Millipore, Merck, USA) covering the base of the microcosm to provide additional spatial structure ...
-
bioRxiv - Genomics 2023Quote: ... 20 µL of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2x final concentration of Protease Inhibitor Cocktail Set 1 (Merck Chemicals). After centrifugation (5 min 15000g) ...
-
bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
-
bioRxiv - Plant Biology 2023Quote: ... then 200 µl of each sample was digested for 16 h at 37 °C with α-amyloglucosidase (6 U/ml, Merck), and α-amylase (4 U/ml ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
-
bioRxiv - Biochemistry 2023Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...
-
bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
-
bioRxiv - Biophysics 2021Quote: ... Immuno-identification of the samples was done using α-c-Myc rabbit antibody and followed by a secondary HRP-conjugated α-rabbit antibody (Merck). α-Histone H3 rabbit antibody was used as housekeeping protein ...
-
bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
-
bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
-
bioRxiv - Cell Biology 2020Quote: U-2-OS Tet-On cell lines expressing eGFP-TopBP1 WT were grown under standard conditions (37°C, 5% CO2) in modified McCoy’s 5a medium (Merck-Sigma-Aldrich, M9309) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Neuroscience 2021Quote: ... the slices were processed with 1% H2O2 in phosphate buffer to deactivate the intrinsic peroxidase and then incubated with an anti-c-Fos antibody (1:10,000, rabbit; Merck KGaA, Darmstadt, Germany) in 10% normal goat serum in phosphate-buffered saline with 0.3% Triton X-100 (PBS-X ...
-
bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
-
bioRxiv - Biochemistry 2021Quote: ... and subsequent thorough rinsing with ultrapure water (18.2 MΩ at 25 °C, maximum 2 ppb total organic carbon, Milli-Q Integral 5 Water Purification System, Merck KGaA, Darmstadt, Germany).
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were pre-treated with (1) 36 µl of Lysozyme [1% w/v in 1% PBS – 37°C/30min with intermittent shaking] (Merck KGaA, Germany) and then with (2 ...
-
bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
-
bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
-
bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
-
bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
-
bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
-
bioRxiv - Cell Biology 2022Quote: ... Ovaries were dissected and oocytes recovered by mouth- pipetting follicles through a narrow glass pipet.Prophase I oocytes were cultured at 38° C in drops of M2 or M16 medium (homemade) supplemented with dbcAMP (dibutyryl cyclic AMP, Sigma Aldrich Merck, D0260) and covered with mineral oil (Sigma-Aldrich Merck ...
-
bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
-
bioRxiv - Microbiology 2020Quote: ... water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). Indole-3-acetic-2,2-d2 acid (≥ 98% ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
-
bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
-
bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
-
bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The prepared sections on glass slides were deparaffinized for 30 minutes at 65°C and then for 2x 30 minutes in Neo-Clear (Merck Millipore, 109843). Samples were rehydrated for 2x 10 minutes in 100% ethanol ...
-
bioRxiv - Genomics 2021Quote: ... Each lobe was segmented so cryosections would fit on the 6,200 x 6,400 µm areas of the Codelink-activated microscope slides and frozen in −30°C 2-Methylbutane (Merck, cat.no.: M32631-1L). The frozen liver samples were embedded in cryomolds (10×10 mm ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Polar metabolites were derivatized for 90 min at 37 °C with 20 μl of 20 mg per ml methoxyamine (Merck Sigma, 226904) in pyridine (Merck Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... Water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). For laboratory culture samples ...
-
bioRxiv - Cell Biology 2022Quote: ... BDNFΔC-ter or Cytochrome-C (negative control which has a molecular weight and a pHi similar to those of BDNF; C2037; Sigma-Aldrich/Merck group) were injected on immobilized KEAP1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated 48 hours at 37°C with BrdU (15 μM, stock solution 30 mM in H2O) (Merck, Darmstadt, Germany). After washing two times with warm PBS the cells were pre-extracted with ice cold NuEx buffer and fixed with 2% (w/v ...
-
bioRxiv - Physiology 2022Quote: ... and separation was carried out at 40°C using a ZIC-HILIC column (2.1 mm × 100 mm, 3 μm; Merck Sequant, Umea, Sweden). Isocratic elution was performed ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were subjected to sonication on ice (10 cycles consisting of 10-s impulses at an output 10% followed by 20-s intervals) followed by incubation for 20 min at 37 °C in the presence of micrococcal nuclease (2 units/sample; Merck, cat. # N3755) and CaCl2 (at 10 mM) ...
-
bioRxiv - Cancer Biology 2022Quote: ... (MW 360 kDa) and (Hydroxypropyl)-methylcellulose (HPMC) (viscosity 40-60 cP, 2 % in H2O (20 C) were purchased from Merck (Darmstadt, Germany). Methanol ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
-
bioRxiv - Biochemistry 2023Quote: ... The culture was grown for 2 hr at 37°C and stocks were made from the original plate by adding glycerol (Merck & Co., USA) to the final concentration of 15% and stored at -70°C ...
-
bioRxiv - Microbiology 2023Quote: ... proteins were ultracentrifuged at 13000 rpm for 10 min at 4°C and concentrated to 1.5 ml using centrifugal filters (Merck, Amicon Ultra-4). They were then exchanged into buffer #5 overnight at 4°C and aliquoted and stored at -80°C ...
-
bioRxiv - Genetics 2023Quote: ... after adding 2 g activated (i.e., baked at 300°C for 2h) molecular sieves (5Å, 45-60 mesh size, Merck, KGaA, Darmstadt, Germany). The molecular sieves were filtered out by loading the extract into a glass funnel (50 mm inner diameter ...
-
bioRxiv - Neuroscience 2022Quote: ... freezed 15 min at −80°C and thawed for 15 min at room temperature following overnight incubation in 2% TritonX-100 (Merck Millipore, 1086031000) PBS for permeabilization ...
-
bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
-
bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...